rBC2LCN-FITC (AiLecS1-FITC) 人iPS未分化标记染料rBC2LCN-FITC (AiLecS1-FITC) 品牌:FUJIFILM Wako


rBC2LCN-FITC (AiLecS1-FITC)

人iPS未分化标记染料rBC2LCN-FITC (AiLecS1-FITC)

品牌:FUJIFILM Wako
CAS No.:
储存条件:-20℃
纯度:
产品编号

(生产商编号)

等级 规格 运输包装 零售价(RMB) 库存情况 参考值

186-02993

for Cell Staining 100uL×5 10,740.00


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人iPS细胞酶试剂盒Takara Clontech

上海金畔生物科技有限公司代理Takara Clontech酶试剂盒全线产品,欢迎访问官网了解更多产品信息和订购。

人iPS细胞
品牌 Code No. 产品名称 包装量 价格(元) 说明书 数量
Cellartis Y00185 Cellartis® human iPS cell line P11025 Kit 1 Kit ¥23,632 人iPS细胞 人iPS细胞 人iPS细胞
Cellartis Y00275 Cellartis® human iPS cell line 7 (ChiPSC7) Kit 1 Kit ¥23,108 人iPS细胞 人iPS细胞 人iPS细胞
Cellartis Y00285 Cellartis® human iPS cell line 12 (ChiPSC12) Kit 1 kit ¥23,108 人iPS细胞 人iPS细胞 人iPS细胞
Cellartis Y00315 Cellartis® human iPS cell line 21 (ChiPSC21) Kit 1 Kit ¥23,108 人iPS细胞 人iPS细胞 人iPS细胞
Cellartis Y00325 Cellartis® human iPS cell line 22 (ChiPSC22) Kit 1 kit ¥23,108 人iPS细胞 人iPS细胞 人iPS细胞
Cellartis Y00305 Cellartis® human iPS cell line 18 (ChiPSC18) Kit 1 Kit ¥23,108 人iPS细胞 人iPS细胞 人iPS细胞
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无标题文档

人iPS细胞
(Human Induced Pluripotent Stem Cells)
Cellartis人iPS细胞(Human iPS Cell Line)产品是达到了工业客户使用标准生产的干细胞产品。该产品包括细胞和用于增殖培养的Cellartis DEF-CS Culture System培养体系。iPS细胞已经在制品化时完成了对DEF-CS培养体系的适应。Cellartis DEF-CS Culture System培养体系是组分明确而且不依赖饲养层的便捷的干细胞培养体系。Cellartis DEF-CS Culture System适合高效率地增殖培养干细胞,并且保持干细胞在增殖过程中几乎无分化地生长,这使得不需要在大规模增殖培养过程中进行细胞筛选。因此,Cellartis人iPS细胞(Human iPS Cell Line)产品是一套完整的产品体系,对于科研级别和工业规模的需求都适合。
 
■ 产品特点
· Cell lines were created by reprogramming human skin fibroblasts using defective polycistronic retrovirus technology to deliver Oct-4, SOX2, KLF4, and c-Myc
· Cell lines are extensively analyzed for purity and stem-cell characteristics, including recovery after thawing, absence of mycoplasma and bacteria, expression of stem cell-specific markers (Oct-4, NANOG, SSEA-3, SSEA-4, TRA-1-60, TRA-1-81), absence of differentiation markers (Beta-3 tubulin, FOXA2, ASMA), and expected karyotype
· Optimized for culture with the Cellartis DEF-CS Culture System
· Suitable for all major applications; cells can form derivatives of all three germ layers when differentiated
· Kits include the Cellartis DEF-CS 100 Culture System
 
■ 关联产品
Code No. Product Size
Y30010 Cellartis® DEF-CS 500 Culture System 1 Kit
 
■ 产品详情请点击:人iPS细胞
 
人iPS细胞
Characteristics of Cellartis human iPS cells. The cell lines were generated from fibroblasts using defective polycistronic retrovirus technology to deliver Oct-4, Sox-2, KLF-4, and c-Myc. The skin fibroblasts were obtained from donors of varying age and gender, have a normal karyotype, and have been confirmed to differentiate into specific cell lineages. Consent was obtained from all donors for commercial use.
 
参考文献:
1. de Peppo GM, et al. Osteogenic potential of human mesenchymal stem cells and human embryonic stem cell-derived mesodermal progenitors: a tissue engineering perspective. Tissue Eng Part A.2010 Nov;16(11):3413-3426.
2. Both SK, et al. Differential bone-forming capacity of osteogenic cells from either embryonic stem cells or bone marrow-derived mesenchymal stem cells. J Tissue Eng Regen Med.2011 Mar;5(3):180-190.
3. de Peppo GM, et al. Free-form-fabricated commercially pure Ti and Ti6A14V Porous Scaffolds support the growth of human embryonic stem cell-derived mesodermal progenitors. ScientificWorldJournal. 2012;2012:646417.Epub 2012 Jan 4.
4. Ou Li, et al. Human embryonic stem cell-derived mesenchymal stroma cells(hES-MSCs) engraft in vivo and support hematopoiesis without suppression immune function: implications for off –the shelf ES-MSC therapies. PLOS ONE.2013; 8(1):e55319.
 

IPS-Spheres 品牌:Reprocell


IPS-Spheres

品牌:Reprocell
CAS No.:
储存条件:+4°C
纯度:
产品编号

(生产商编号)

等级 规格 运输包装 零售价(RMB) 库存情况 参考值

BR-01S-020

20 mg 咨询


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* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动,恕不另行通知,确切详情请联系上海金畔生物科技有限公司。

人iPS细胞来源肝脏细胞酶试剂盒Takara Clontech

上海金畔生物科技有限公司代理Takara Clontech酶试剂盒全线产品,欢迎访问官网了解更多产品信息和订购。

人iPS细胞来源肝脏细胞
品牌 Code No. 产品名称 包装量 价格(元) 说明书 数量
Cellartis Y10133 Cellartis® Enhanced hiPS-HEP v2 (from ChiPSC12) Kit 1 kit ¥21,173 人iPS细胞来源肝脏细胞 人iPS细胞来源肝脏细胞 人iPS细胞来源肝脏细胞
Cellartis Y10134 Cellartis® Enhanced hiPS-HEP v2 (from ChiPSC18) Kit 1 kit ¥21,173 人iPS细胞来源肝脏细胞 人iPS细胞来源肝脏细胞 人iPS细胞来源肝脏细胞
Cellartis Y10135 Cellartis® Enhanced hiPS-HEP v2 (from ChiPSC22) Kit 1 kit ¥21,173 人iPS细胞来源肝脏细胞 人iPS细胞来源肝脏细胞 人iPS细胞来源肝脏细胞
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人iPS干细胞分化来的肝细胞
Cellartis enhanced hiPS-HEP v2 kits 提供了可持续供应的、成熟的人诱导多能干细胞(hiPS)来源的肝脏细胞。在超过14天的检测窗口期,hiPS-HEP细胞拥有功能性的药物代谢机制,使其成为体外药物研发、药物代谢、和相关毒性研究的理想选择;具备成熟、稳健的代谢特征,支持其作为代谢疾病模型。方便使用的试剂盒包括完全的培养基和组份,用于长期检测。与其他肝脏细胞模型相比,培养的Cellartis hiPS-HEP细胞存活时间更长,进而拥有更长的检测窗口用于慢性-毒性实验和作为代谢疾病模型研究。
 
■ 产品特点
· Complete kit for thawing, plating, and culturing the provided cells
· Highly homogeneous population with consistent performance between batches and over time
· Cells are functional and stable for a 14-day assay window
· Cells show long-term, stable CYP450 activities
· Cells express key hepatocyte proteins, including α1AT, Alb, CK18, HNF4α, and E-cadherin
· Cells secrete physiologically relevant levels of albumin and urea
· Cells demonstrate functional insulin and glucose regulation
· Cells show phase I and II enzyme activities
· Cells demonstrate LDL uptake
 
■ 产品组成
· 1 vial of ~1.2 × 107 cells
· Hepatocyte thawing medium
· Hepatocyte coating
· Hepatocyte plating medium
· Hepatocyte washing medium
· Hepatocyte long-term maintenance medium
 
■ 产品应用
· Toxicity testing, including chronic toxicity assays
· Drug discovery and development
· Metabolic studies
· Metabolic disease modeling
 
■ 产品详情请点击:人iPS细胞来源肝脏细胞
 
人iPS细胞来源肝脏细胞
 
Cellartis 人iPS细胞来源的肝脏细胞可以作为长期的、可靠的肝脏疾病模型。
Cellartis human induced pluripotent stem (iPS) cell-derived hepatocytes are a long-lasting, reliable liver disease model. Ideal for long-term studies, these mature, functional, pure hepatocytes allow you to generate consistent results with low batch-to-batch variability. Cellartis iPSC-derived hepatocytes can be used for extended culture significantly longer than human primary hepatocytes—allowing you to get more data from your chronic toxicity studies.
 
人iPS细胞来源肝脏细胞
 
Cellartis enhanced hiPS-HEP cells可以稳定表达CYP450 活性超过21天。
CYP450 activity is stable in enhanced hiPS-HEP cells over 21 days. LC/MS was used to analyze CYP450 activity in cultured enhanced hiPS-HEP cells previously derived from the hiPS cell lines ChiPSC12, ChiPSC18, and ChiPSC22 (abbreviated as C12, C18, and C22). CYP3A (Panel A), CYP2C9 (Panel B), CYP1A (Panel C), and CYP2C19 (Panel D) activities in enhanced hiPS-HEP cells are stable over an extended culture time. Cryopreserved human primary hepatocytes (hphep), which are functional in culture for a significantly shorter time than enhanced hiPS-HEP cells, were thawed and cultured for 20 hours, and their data are shown as the black bar in each panel.
 
人iPS细胞来源肝脏细胞
 
Cellartis enhanced hiPS-HEP细胞在20天的培养中表达白蛋白(Albumin)。
Albumin is present in enhanced hiPS-HEP cells through 20 days in culture. Panel A. Representative images of enhanced hiPS-HEP cells from human iPS cell line ChiPSC18 (C18) taken 12 days after thawing (right), compared to cryopreserved human primary hepatocytes (hphep) taken 24 hr after thawing (left). Cells were stained for albumin and DAPI. Panel B. Albumin secretion as measured by ELISA; n=2 for enhanced hiPS-HEP cells, with the exception of C18 at 20 days (n=1), and n=3 donors for hphep.
 
(以上图片均来源于Takara Bio USA, Inc.)
 
参考文献:
1. Heins et al. Stem Cells 2004; 22: 367-376.United States National Stem Cell Bank; http://www.nationalstemcellbank.org.
2. Mantel N et al. Potential markers of attenuation of YF virus after infection of stem cell-derived human hepatocytes with wild-type Asibi or live-attenuated YF17D virus.Supplement to The American Journal of tropical Medicine and Hygiene, Volume 83, November 2010, Number 5, abstract 12.
3. Yildirimman R et al. Human embryonic stem cell derived hepatocyte-like cells as a tool for in vitro hazard assessment of chemical carcinogenicity. Toxicol. Sci. 2011 Dec; 124(2): 278-90.
4. Ulvestad M et al. Drug metabolizing enzyme and transporter protein profi les of hepatocytes derived from human embryonic and induced pluripotent stem cells. Biochem Pharmacol. 2013 Sep 1; 86(5):691-702.
 
 

IPS-Spheres 品牌:Reprocell


IPS-Spheres

品牌:Reprocell
CAS No.:
储存条件:+4°C
纯度:
产品编号

(生产商编号)

等级 规格 运输包装 零售价(RMB) 库存情况 参考值

BR-01S-150

150 mg 咨询


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动,恕不另行通知,确切详情请联系上海金畔生物科技有限公司。