Harlan TD.88137 高脂饲料 Adjusted Kcal Diet （42% from fat)
Harlan Teklad动物饲料
Harlan代理
欢迎新老客户访问Harlan官网或者咨询中国代理商上海金畔生物获取更多详细资料。
Harlan TD.88137 高脂饲料 Adjusted Kcal Diet （42% from fat)
A staple of atherosclerosis research
More than 25 years ago, our nutritionists collaborated with researchers at Rockefeller University to develop a diet with features of a ‘Western Diet’ to characterize and enhance atherosclerosis development in their newly generated Apoe deficient mouse model. With over 200 unique users worldwide, TD.88137 continues to be fed to genetically modified cardiovascular models to accelerate and enhance hypercholesterolemia and plaque formation.
Formula                                             g/Kg
Casein                                              195.0
DL-Methionine                                     3.0
Sucrose                                            341.46
Corn Starch                                       150.0
Andydrous Milkfat                               210.0
Cholesterol                                          1.5
Cellulose                                              50.0
Mineral Mix, AIN-76 (170915)               35.0
Calcium Carbonate                               4.0
Vitamin Mix, Teklad (40060)                10.0
Ethoxyquin                                          0.04
Critical dietary features of TD.88137 related to atherosclerosis development include:
++Cholesterol (0.2% total cholesterol)
++Total fat (21% by weight; 42% kcal from fat)
++High in saturated fatty acids  (>60% of total fatty acids)
++High sucrose (34% by weight)
Typical fatty acid profile of TD.88137
Typical fatty acid analysis,% of diet1                   Mean                             SD
Total                                                                    20.7                                1.5
Saturated fat                                                       12.8                                0.8
Monounsaturated fat                                             5.6                                0.5
Polyunsaturated fat                                             1.0                                  0.2
Unknown2                                                           1.3                                   0.3
Selected nutrient information1
Key points from the literature
TD.88137 has been used to accelerate atherosclerosis development in Apoe and Ldlr deficient models:
++In Apoe deficient mice, plasma cholesterol triples to >1500 mg/dL within three weeks (1, 2). Foam cell and lesion development occurs within 6-10 weeks
(2-4). Fibrous plaque formation is observed at 15 weeks with the development of fibrous caps after 20 weeks (2).
++Ldlr deficient mice fed for two weeks increase plasma cholesterol to >800 mg/dL and triglyceride to >300 mg/dL (5). After six weeks of feeding, hyperglycemia, hyperinsulinemia and dyslipidemia develop with small foam cell lesions in the aortic
arch (6, 7).
With over 420 citations, uses of TD.88137 continue to evolve and include atherosclerosis, obesity, non-alcoholic steatohepatitis (NASH), osteoporosis,
hypertension and metabolic syndrome. Contact us for a more extensive reference list.
Control diet options for TD.88137
Natural ingredient diets
++Also referred to as standard diets or chow
++Diets differ in the source and level of nutrients as well as the presence of non-nutritive factors (such as phytates or phytoestrogens) compared
to TD.88137
++Limits inferences to differences in dietary pattern versus a specific dietary component
Ingredient matched, low fat diets
++Controls for the type of ingredients, non-nutritive components and the source and level of specific nutrients
++Suggested ingredient matched, low fat dietary controls for TD.88137 listed below; data sheets can be found on our website at envigo.com
Suggested ingredient matched, low fat controls

References  参考文献
1. Plump, A.S., et al., Severe hypercholesterolemia and atherosclerosis in
apolipoprotein E-deficient mice created by homologous recombination in
ES cells. Cell, 1992. 71(2): p. 343-53.
2. Nakashima, Y., et al., Apoe-deficient mice develop lesions of all phases of
atherosclerosis throughout the arterial tree. Arterioscler Thromb, 1994.
14(1): p. 133-40.
3. Febbraio, M., et al., Targeted disruption of the class B scavenger receptor
CD36 protects against atherosclerotic lesion development in mice.
J Clin Invest, 2000. 105(8): p. 1049-56.
4. Nakashima, Y., et al., Upregulation of VCAM-1 and ICAM-1 at atherosclerosis-prone
sites on the endothelium in the Apoe-deficient mouse. Arterioscler Thromb
Vasc Biol, 1998. 18(5): p. 842-51.
5. Towler, D.A., et al., Diet-induced diabetes activates an osteogenic gene regulatory
program in the aortas of low density lipoprotein receptor-deficient mice.
J Biol Chem, 1998. 273(46): p. 30427-34.
6. Tsuchiya, K., et al., FoxOs integrate pleiotropic actions of insulin in vascular
endothelium to protect mice from atherosclerosis. Cell Metab, 2012. 15(3): p. 372-81.
7. Huszar, D., et al., Increased LDL cholesterol and atherosclerosis in LDL receptordeficient
mice with attenuated expression of scavenger receptor B1. Arterioscler
Thromb Vasc Biol, 2000. 20(4): p. 1068-73.
8. Yang, B., et al., Changes of skeletal muscle adiponectin content in diet-induced
insulin resistant rats. Biochem Biophys Res Commun, 2006. 341(1): p. 209-17.
9. Schafer, K., et al., Leptin promotes vascular remodeling and neointimal growth
in mice. Arterioscler Thromb Vasc Biol, 2004. 24(1): p. 112-7.
10. Lijnen, H.R., et al., Nutritionally induced obesity is attenuated in transgenic mice
overexpressing plasminogen activator inhibitor-1. Arterioscler Thromb Vasc
Biol, 2003. 23(1): p. 78-84.
11. Maquoi, E., et al., Modulation of adipose tissue expression of murine matrix
metalloproteinases and their tissue inhibitors with obesity. Diabetes, 2002.
51(4): p. 1093-101.
12. VanSaun MN, et al. 2009. High fat diet induced hepatic steatosis establishes a
permissive microenvironment for colorectal metastases and promotes primary
dysplasia in a murine model. Am J Pathol 175:355-64.
13. Dixon LJ, et al. 2013. Caspase-1 as a central regulator of high fat diet-induced
non-alcoholic steatohepatitis. PLoS One 8:e56100.

Harlan矿物质调节饲料Mineral adjusted diets
Harlan Teklad动物饲料
Harlan代理
欢迎新老客户访问Harlan官网或者咨询中国代理商上海金畔生物获取更多详细资料。
Mineral adjusted diets
A few mineral adjusted formulas are shown below. Please contact us for additional formulas of this nature or for more information about altering the mineral profile of a diet. Read below for more information.
Formula examples:
TD.80396      Iron deficient diet
TD.85419      Zinc deficient diet
TD.88239      Potassium deficient diet
TD.95027      Low calcium diet (0.4% P)
TD.96348      Diet (20% lactose, 2% Ca, 1.25% P)
TD.92163      Selenium deficient diet
TD.95125      Iodine deficient (0.15% PTU)
Unique protein ingredients may be necessary to minimize background levels of certain minerals. Other steps taken to minimize background mineral contamination include the use of reagent grade minerals when necessary or omission of certain ingredients. Specialized mineral premixes may be used in these diets, or minerals are added individually to the diet in order to control the level of specific minerals.
With the exception of sodium, it is necessary to use refined ingredients to achieve the lowest possible levels of minerals in a diet.
From this mineral deficient base, various levels of minerals can be added back. Some formulas adjust multiple minerals, and other related nutrients.
Contact us to discuss other possibilities for the minerals listed above, and for those minerals not listed here.
一些矿物调整公式如下所示。请与我们联系，以获得更多的这种性质的配方，或更多的信息，改变矿物质的饮食。有关更多信息，请阅读下面。
公式示例：
TD.80396缺铁饮食
TD.85419缺锌饮食
TD.88239缺钾饮食
TD.95027低钙饮食(0.4%P)
TD.96348日粮(20%乳糖，2%钙，1.25%P)
TD.92163缺硒饮食
TD.95125缺碘(0.15%PTU)
独特的蛋白质成分可能是必要的，以尽量减少某些矿物质的背景水平。为尽量减少背景矿物污染而采取的其他步骤包括在必要时使用试剂级矿物或遗漏某些成分。在这些饮食中可以使用特殊的矿物预混料，也可以在饮食中单独添加矿物质，以控制特定矿物质的含量。
除钠外，必须使用精制成分，以达到饮食中矿物质含量尽可能低的水平。
从这种矿物缺乏的基础，不同水平的矿物可以补充回来。一些配方调整多种矿物质和其他相关营养素。
上海金畔生物科技有限公司（www.jinpanbio.com）提供生命科学研究领域系列产品，包括生化试剂、诊断试剂、色谱标准品和实验仪器耗材。主营Lumiprobe Cy系列活性荧光染料；修饰性PEG（Laysan bio、NANOCS、Avanti等进口品牌PEG以及定制合成修饰性聚乙二醇、单分散小分量PEG）；Sigma、Amresco、TCI、MP bio生化试剂；WAKO日本和光纯药、日本关东化学Kanto试剂、日本三菱、日本柴田科学SIBATA；Megazyme食品分析检测试剂盒、日本共立理化学；Research diets、Harlan饲料、Bio-Serv、日本CLEA Japan品牌的动物饲料；Oxoid、Nissui日水、日本荣研、BD difco、Himedia品牌微生物培养基；免疫诊断试剂包括:Bethyl抗体；Biolegend流式抗体、Abcam、CST、Santa Cruz抗体；Roche、TOYOBO、NEB品牌的酶；中检所、TRC、药典USP、EP、Reagecon标准品；耗材和仪器包括Whatman、日本Advantec滤膜、Millipore品牌的各种滤膜、滤器和柱子填料等、Hampton蛋白结晶试剂耗材、老鼠软管灌胃针、动物毛发记号笔、Labnet、Wheaton瓶子、Bio-Rad伯乐、康宁Corning、Axygen、Falcon 、Eppendorf、Nunc、Nalgene、Nest品牌的培养皿、培养板、离心机、离心管、移液枪及枪头等实验室常用仪器耗材。
上海金畔生物科技有限公司
服务热线:18301939375   QQ：3258089810    3259632176
Email:  info@jinpanbio.com
官网：http://www.jinpanbio.com

Harlan蛋白质调节饲料 Protein adjusted diets
Teklad diet, bedding and enrichment + Teklad laboratory animal diets + Custom research diets + Protein adjusted diets
Harlan Teklad动物饲料
Harlan代理
欢迎新老客户访问Harlan官网或者咨询中国代理商上海金畔生物获取更多详细资料。
Harlan蛋白质调节饲料 Protein adjusted diets
Protein adjusted diets | Envigo
A few protein adjusted formulas are shown below. Please contact us for additional formulas of this nature or for more information about altering the protein type or content of a diet.
Formula examples:
TD.93328      “Protein free” diet
TD.90016      6% Protein diet
TD.91352       20% Protein diet
TD.90018      40% Protein diet
These diet examples are from a series of diets with the following features: casein-based, isocaloric (3.8 kcal/g) and matched for fat (5.5%), calcium (0.7%), and phosphorus (0.54%).  There are other diets available from this series of diets, as well as other unique protein adjusted diets.
Casein is the most widely used refined protein source, but it is also possible to use other refined protein sources, such as “vitamin-free” test casein (alcohol extracted), lactalbumin, isolated soy protein, and egg white solids. These particular protein sources are usually used to achieve a specific research objective, rather than for routine use. For instance, vitamin-free test casein is most appropriately used in vitamin deficient diets, and egg white solids is most suitable for zinc or biotin deficient diets.
Adjusted protein diets usually range from “protein-free” (trace) to 60% protein, depending on the research.
一些蛋白质调整的公式如下所示。请与我们联系，以获得更多的这种性质的配方，或更多有关改变蛋白质类型或饮食内容的信息。
公式示例：
TD.93328“无蛋白质”饮食
TD.90016  6%蛋白质饮食
TD.91352  20%蛋白质饮食
TD.90018  40%蛋白质饮食
这些饮食的例子来自一系列的饮食，有以下特点：酪蛋白为基础，等热量(3.8千卡/克)，与脂肪(5.5%)，钙(0.7%)和磷(0.54%)匹配。从这一系列的饮食中可以得到其他的饮食，以及其他独特的蛋白质调整的饮食。
酪蛋白是最广泛使用的精制蛋白质来源，但也可以使用其他精制蛋白质来源，如“无维生素”测试酪蛋白(醇提)，乳清蛋白，分离大豆蛋白和蛋清固体。这些特定的蛋白质来源通常用于实现特定的研究目标，而不是用于常规用途。例如，无维生素测试酪蛋白最适合用于缺乏维生素的饮食，而蛋清固体最适合于锌或生物素缺乏的饮食。
根据研究结果，调整后的蛋白质饮食通常从“无蛋白质”(微量)到60%的蛋白质。
上海金畔生物科技有限公司（www.jinpanbio.com）提供生命科学研究领域系列产品，包括生化试剂、诊断试剂、色谱标准品和实验仪器耗材。主营Lumiprobe Cy系列活性荧光染料；修饰性PEG（Laysan bio、NANOCS、Avanti等进口品牌PEG以及定制合成修饰性聚乙二醇、单分散小分量PEG）；Sigma、Amresco、TCI、MP bio生化试剂；WAKO日本和光纯药、日本关东化学Kanto试剂、日本三菱、日本柴田科学SIBATA；Megazyme食品分析检测试剂盒、日本共立理化学；Research diets、Harlan饲料、Bio-Serv、日本CLEA Japan品牌的动物饲料；Oxoid、Nissui日水、日本荣研、BD difco、Himedia品牌微生物培养基；免疫诊断试剂包括:Bethyl抗体；Biolegend流式抗体、Abcam、CST、Santa Cruz抗体；Roche、TOYOBO、NEB品牌的酶；中检所、TRC、药典USP、EP、Reagecon标准品；耗材和仪器包括Whatman、日本Advantec滤膜、Millipore品牌的各种滤膜、滤器和柱子填料等、Hampton蛋白结晶试剂耗材、老鼠软管灌胃针、动物毛发记号笔、Labnet、Wheaton瓶子、Bio-Rad伯乐、康宁Corning、Axygen、Falcon 、Eppendorf、Nunc、Nalgene、Nest品牌的培养皿、培养板、离心机、离心管、移液枪及枪头等实验室常用仪器耗材。
上海金畔生物科技有限公司
服务热线:18301939375   QQ：3258089810    3259632176
Email:  info@jinpanbio.com
官网：http://www.jinpanbio.com

Harlan 维生素调整饲料 Vitamin adjusted diets
Harlan Teklad动物饲料
Harlan代理
欢迎新老客户访问Harlan官网或者咨询中国代理商上海金畔生物获取更多详细资料。
A few vitamin adjusted formulas are shown below. Please contact us for additional formulas of this nature or for more information about altering the vitamin profile of a diet, and please read below to learn more.
Formula Examples:
TD.86143 Vitamin A deficient diet
TD.89123 Vitamin D deficient diet
TD.88163 Vitamin E deficient diet
TD.95247 Folic acid deficient diet
Most vitamin deficient diets use vitamin-free test casein (VFT casein) as the protein source. VFT casein is casein that has been extracted with denatured alcohol to reduce the fat content from about 1% to 0.1%. In the process the content of a number of vitamins (present at low or trace levels in casein) is further reduced, making this an ideal protein source for these types of diets. Envigo produces our own VFT in-house, and we also offer this as an ingredient for those mixing their own diets.
From this vitamin deficient base, various levels of vitamins can be added back. Many formulas adjust multiple vitamins, and other nutrients.
If use of an intact protein source is not advisable, an amino acid defined diet can be used.
一些维生素调整公式如下所示。请与我们联系，以获得更多的这种性质的配方或更多的信息，以改变维生素的饮食概况，并请阅读下面来了解更多。
公式示例：
TD.86143维生素A缺乏饮食
TD.89123维生素D缺乏饮食
TD.88163维生素E缺乏饮食
TD.95247叶酸缺乏饮食
大多数缺乏维生素的饮食使用无维生素的测试酪蛋白(VFT酪蛋白)作为蛋白质来源。VFT酪蛋白是用变性醇提取的酪蛋白，使脂肪含量从1%左右降至0.1%左右。在这个过程中，一些维生素的含量(在低水平或微量的酪蛋白)被进一步减少，使之成为这些类型的饮食的理想的蛋白质来源。Envigo生产我们自己的VFT内部产品，我们也为那些混合他们自己的饮食的人提供这种材料。
从这个缺乏维生素的基础，不同水平的维生素可以补充回来。许多配方调整多种维生素和其他营养。
如果使用完整的蛋白质源是不可取的，氨基酸限定饮食可以使用。
上海金畔生物科技有限公司（www.jinpanbio.com）提供生命科学研究领域系列产品，包括生化试剂、诊断试剂、色谱标准品和实验仪器耗材。主营Lumiprobe Cy系列活性荧光染料；修饰性PEG（Laysan bio、NANOCS、Avanti等进口品牌PEG以及定制合成修饰性聚乙二醇、单分散小分量PEG）；Sigma、Amresco、TCI、MP bio生化试剂；WAKO日本和光纯药、日本关东化学Kanto试剂、日本三菱、日本柴田科学SIBATA；Megazyme食品分析检测试剂盒、日本共立理化学；Research diets、Harlan饲料、Bio-Serv、日本CLEA Japan品牌的动物饲料；Oxoid、Nissui日水、日本荣研、BD difco、Himedia品牌微生物培养基；免疫诊断试剂包括:Bethyl抗体；Biolegend流式抗体、Abcam、CST、Santa Cruz抗体；Roche、TOYOBO、NEB品牌的酶；中检所、TRC、药典USP、EP、Reagecon标准品；耗材和仪器包括Whatman、日本Advantec滤膜、Millipore品牌的各种滤膜、滤器和柱子填料等、Hampton蛋白结晶试剂耗材、老鼠软管灌胃针、动物毛发记号笔、Labnet、Wheaton瓶子、Bio-Rad伯乐、康宁Corning、Axygen、Falcon 、Eppendorf、Nunc、Nalgene、Nest品牌的培养皿、培养板、离心机、离心管、移液枪及枪头等实验室常用仪器耗材。
上海金畔生物科技有限公司
服务热线:18301939375   QQ：3258089810    3259632176
Email:  info@jinpanbio.com
官网：http://www.jinpanbio.com