日本关东化学Kanto 37565-79 Silica gel 60N, spherical neutral, particle size 63‐210μm

日本关东化学Kanto 37565-79 Silica gel 60N, spherical neutral, particle size 63‐210μm
日本关东化学代理商–上海金畔生物
代理优势:货期2-3周。
欢迎访问关东化学株式会社Kanto官网或者咨询我们获取更多产品信息。
日本关东化学Kanto 37565-79 Silica gel 60N, spherical neutral, particle size 63‐210μm

Product No. 37565-79
Product name Silica gel 60N, spherical neutral, particle size 63‐210μm
Grade for column chromatography
Package 1kg
Price ¥9,000
Stock 100<=
Product instructions particle size 63μm~210μm
CAS No. 7631-86-9

日本关东化学Kanto 7631-86-9 二氧化硅
二氧化硅(IV), 40%水胶态分散体 Silicon dioxide
CasNo:7631-86-9  MDL:MFCD06660073  分子式:O2 Si  分子量:60.0800

Product No. Product Name Grade Package
37038-02 Silica gel, Blue, big granule   500g
37038-84 Silica gel, Blue, big granule   13.5kg
37039-02 Silica gel, Blue, middle granule   500g
37039-84 Silica gel, Blue, middle granule   13.5kg
37044-02 Silica gel, White, big granule   500g
37045-02 Silica gel, White, middle granule   500g
37046-02 Silica gel, White, small granule   500g
37047-02 Silica gel, Orange, middle granule   500g
37047-82 Silica gel, Orange, middle granule   12.5kg
37489-08 Silica gel, Blue, middle granule, NeoBLUER for drying 500g
37489-84 Silica gel, Blue, middle granule, NeoBLUER for drying 12.5kg
37556-96 Silica gel 60N,spherical neutral,particle size 40‐50μm,60mL for flash chromatography 20pieces
37558-79 Silica gel 60, spherical, particle size 100‐210μm for column chromatography 1kg
37558-84 Silica gel 60, spherical, particle size 100‐210μm for column chromatography 10kg
37558-85 Silica gel 60, spherical, particle size 100‐210μm for column chromatography 25kg
37559-79 Silica gel 60, spherical, particle size 40‐100μm for flash chromatography 1kg
37559-84 Silica gel 60, spherical, particle size 40‐100μm for flash chromatography 10kg
37559-85 Silica gel 60, spherical, particle size 40‐100μm for flash chromatography 25kg
37560-79 Silica gel 60N, spherical neutral, particle size 100‐210μm for column chromatography 1kg
37560-84 Silica gel 60N, spherical neutral, particle size 100‐210μm for column chromatography 10kg
37560-85 Silica gel 60N, spherical neutral, particle size 100‐210μm for column chromatography 25kg
37561-79 Silica gel 60N, spherical neutral, particle size 40‐100μm for flash chromatography 1kg
37561-84 Silica gel 60N, spherical neutral, particle size 40‐100μm for flash chromatography 10kg
37561-85 Silica gel 60N, spherical neutral, particle size 40‐100μm for flash chromatography 25kg
37562-79 Silica gel 60, spherical, particle size 40‐50μm for flash chromatography 1kg
37562-84 Silica gel 60, spherical, particle size 40‐50μm for flash chromatography 10kg
37562-85 Silica gel 60, spherical, particle size 40‐50μm for flash chromatography 25kg
37563-79 Silica gel 60N, spherical neutral, particle size 40‐50μm for flash chromatography 1kg
37563-84 Silica gel 60N, spherical neutral, particle size 40‐50μm for flash chromatography 10kg
37563-85 Silica gel 60N, spherical neutral, particle size 40‐50μm for flash chromatography 25kg
37564-79 Silica gel 60, spherical, particle size 63‐210μm for column chromatography 1kg
37564-84 Silica gel 60, spherical, particle size 63‐210μm for column chromatography 10kg
37564-85 Silica gel 60, spherical, particle size 63‐210μm for column chromatography 25kg
37565-79 Silica gel 60N, spherical neutral, particle size 63‐210μm for column chromatography 1kg
37565-84 Silica gel 60N, spherical neutral, particle size 63‐210μm for column chromatography 10kg
37565-85 Silica gel 60N, spherical neutral, particle size 63‐210μm for column chromatography 25kg
37571-79 Silica gel 60N, irregular neutral, particle size 40‐63μm for flash chromatography 1kg
37572-79 Silica gel 60N, irregular neutral, particle size 63‐200μm for column chromatography 1kg
7730-1M Silica gel 60GF254 (type60) for thin layer chromatography 500g
7730-2M Silica gel 60GF254 (type60) for thin layer chromatography 1kg
7730-3M Silica gel 60GF254 (type60) for thin layer chromatography 5kg
7731-2M Silica gel 60G (type60) for thin layer chromatography 1kg
7731-3M Silica gel 60G (type60) for thin layer chromatography 5kg
7736-2M Silica gel 60H (type60) for thin layer chromatography 1kg
7736-3M Silica gel 60H (type60) for thin layer chromatography 2.5kg
7739-2M Silica gel 60HF254 (type60) for thin layer chromatography 1kg
7747-1M Silica gel 60PF254 for preparative thin layer chromatography 1kg
7747-2M Silica gel 60PF254 for preparative thin layer chromatography 2.5kg
37049-01 Silicon dioxide, amorphous Extra pure 500g
37049-10 Silicon dioxide, amorphous Guaranteed reagent for JIS 250g
37049-13 Silicon dioxide, amorphous, 3N high purity reagent 250g
37049-30 Silicon dioxide, amorphous Guaranteed reagent for JIS 25g
37049-86 Silicon dioxide, amorphous Guaranteed reagent for JIS 5kg
37484-23 Silicon dioxide for advanced material research 100g
37484-43 Silicon dioxide for advanced material research 10g
37484-89 Silicon dioxide for advanced material research 5kg
49525-70 Silicon oxide, powder   25g
49525-71 Silicon oxide, powder   100g
49525-72 Silicon oxide, powder   500g
49525-73 Silicon oxide, powder   1kg
40373-1A CAB‐O‐SILR M‐5 for liquid scintillation counting 150g
37563-23 Silica gel, water content 5% for environmental analysis 100g
24167-1A Silica gel, for chromatography, 0.200‐0.500 mm, 40 A   1kg
24167-2A Silica gel, for chromatography, 0.200‐0.500 mm, 40 A   250g
25509-79 Mightysil Si 60 (40‐50μm) for flash chromatography 1kg
25509-85 Mightysil Si 60 (40‐50μm) for flash chromatography 25kg
41929-1A Silica gel, for chromatography, 0.060‐0.200 mm, 60 A   1kg
41929-2A Silica gel, for chromatography, 0.060‐0.200 mm, 60 A   5kg
41929-3A Silica gel, for chromatography, 0.060‐0.200 mm, 60 A   250g
49525-74 Silicon oxide, powder   25g
49525-75 Silicon oxide, powder   100g
49525-76 Silicon oxide, powder   500g
49525-77 Silicon oxide, powder   1kg

日本关东化学Kanto 氯化钙 CAS 10035-04-8

日本关东化学Kanto 氯化钙 CAS 10035-04-8
日本关东化学代理商–上海金畔生物
代理优势:货期2-3周。
欢迎访问关东化学株式会社Kanto官网或者咨询我们获取更多产品信息。
日本关东化学Kanto 氯化钙 CAS 10035-04-8

Product No. 07058-00
Product name Calcium chloride dihydrate
Grade Guaranteed reagent for JIS
Purity 99.0%~103.0%(T)
Package 500g
Price ¥2,000
Stock 100<=
Product information   JISK8122

日本关东化学Kanto 氯化钙 CAS 10035-04-8

Product No. Product Name Grade Package Stock
07058-00 Calcium chloride dihydrate Guaranteed reagent for JIS 500g 100<=
07058-01 Calcium chloride dihydrate Extra pure 500g 100<=
07058-23 Calcium chloride dihydrate for molecular biology 100g 50<=
07058-80 Calcium chloride dihydrate Guaranteed reagent for JIS 15kg 50<=
07058-81 Calcium chloride dihydrate Extra pure 15kg 50<=
2382-1M Calcium chloride dihydrate EMSURER 250g non-stocked
58001-17 Calcium chloride 0‐2H2O Food additives 500g 100<=
58001-41 Calcium chloride 0‐2H2O Food additives 20kg Contact us
42352-1A Calcium chloride dihydrate   25g non-stocked
42352-2A Calcium chloride dihydrate   500g non-stocked

日本Kanto 关东化学氯化钠 Sodium chloride 7647-14-5

日本Kanto 关东化学氯化钠 Sodium chloride 7647-14-5
日本关东化学代理商–上海金畔生物
代理优势:货期2-3周。
欢迎访问关东化学株式会社Kanto官网或者咨询我们获取更多产品信息。
日本Kanto 关东化学氯化钠 Sodium chloride 7647-14-5

Product No. 37144-00
Product name Sodium chloride
Grade Guaranteed reagent for JIS
Purity >99.5%(T)
Package 500g
Price ¥1,000
Stock 100<=
Product information JISK8150

日本Kanto 关东化学氯化钠 Sodium chloride 7647-14-5

Formula NaCl
Formula weight 58.44
Physical properties mp 801℃
(△/Specification ▲/Average result †/Litureture)
CAS No. 7647-14-5

 

Product No. Product Name Grade Package Stock
08126-23 Chloride ion standard solution JCSS for chemical analysis, for ion chromatography 100mL 100<=
37144-00 Sodium chloride Guaranteed reagent for JIS 500g 100<=
37144-01 Sodium chloride Extra pure 500g 100<=
37144-02 Sodium chloride GR(for medicine analysis) 500g 100<=
37144-09 Sodium chloride for phthalic acid esters analysis 500g 20<=
37144-1B Sodium chloride for pesticide residue & PCB analysis 500g 100<=
37144-23 Sodium chloride for molecular biology 100g 100<=
37144-80 Sodium chloride Guaranteed reagent for JIS 20kg 50<=
37144-81 Sodium chloride Extra pure 20kg 50<=
37144-86 Sodium chloride Guaranteed reagent for JIS 5kg 50<=
37144-88 Sodium chloride Extra pure 5kg 10<=
37144-96 Sodium chloride Reference materials for volumetric analysis 50g 100<=
37504-08 Sodium chloride Biopur 500g 50<=
37834-08 0.1mol/L Sodium chloride solution (N/10) for volumetric titration 500mL 100<=
37835-08 0.02mol/L Sodium chloride solution (N/50) for volumetric titration 500mL 50<=
37836-08 0.01mol/L Sodium chloride solution (N/100) for volumetric titration 500mL 20<=
37918-08 0.05mol/L Sodium chloride solution(N/20) for volumetric titration 500mL Contact us
37926-08 1mol/L Sodium chloride solution (1N) for volumetric titration 500mL 100<=
38129-08 Sodium chloride (UE‐E) Japanese pharmacopoeia ( for manufacture only ) 500g Contact us
38129-84 Sodium chloride (UE‐E) Japanese pharmacopoeia ( for manufacture only ) 10kg Contact us
38146-00 Sodium chloride Conforms to RoHS 500g <10
49005-00 Sodium chloride, CRM3008‐a Certified reference material 50g Contact us
6406-1M Sodium chloride SuprapurR 50g non-stocked
20779-1A Sodium chloride   1kg non-stocked
20779-2A Sodium chloride   5kg non-stocked
20779-3A Sodium chloride   25kg non-stocked
32730-1A Sodium chloride, DNAse, RNAse and Protease free   1kg non-stocked
32730-2A Sodium chloride, DNAse, RNAse and Protease free   2.5kg non-stocked
37144-08 Sodium chloride for Japanese pharmacopoeia 500g 100<=
37897-82 5% Sodium chloride solution   18L Contact us
42429-1A Sodium chloride   1kg non-stocked
42429-2A Sodium chloride   5kg non-stocked
42429-3A Sodium chloride   25g non-stocked
42429-4A Sodium chloride   25kg non-stocked
42429-5A Sodium chloride   500g non-stocked

日本Kanto关东化学 37280-00 硫酸钠

日本Kanto关东化学 37280-00 硫酸钠
日本关东化学代理商–上海金畔生物
代理优势:货期2-3周。
欢迎访问关东化学株式会社Kanto官网或者咨询我们获取更多产品信息。
日本Kanto关东化学 37280-00 硫酸钠

Product No. 37280-00
Product name Sodium sulfate
Grade Guaranteed reagent for JIS
Purity >99.0%(T)
Package 500g
Price ¥1,300
Stock 100<=
Product information JISK8987
Formula Na2SO4
Formula weight 142.04
Physical properties mp 884℃
(△/Specification ▲/Average result †/Litureture)
CAS No. 7757-82-6

日本关东化学 32033-00 Liquid paraffin 8012-95-1

日本关东化学 32033-00 Liquid paraffin 8012-95-1
日本关东化学代理商–上海金畔生物
代理优势:货期2-3周。
欢迎访问关东化学株式会社Kanto官网或者咨询我们获取更多产品信息。

Product No. 32033-00
Product name Liquid paraffin
Grade Guaranteed reagent for JIS
Package 500mL
Price ¥1,800
Stock 100<=
Product information   JISK9003

Byname Paraffin, Liquid
Physical properties Fp 238℃† d (15℃)0.87†
(△/Specification ▲/Average result †/Litureture)
CAS No. 8012-95-1

Wako 322-27792 沸石,HS-642粉末,Na-丝光型

Wako 322-27792 沸石,HS-642粉末,Na-丝光型
Zeolite, Synthetic, HS-642 Powder, Sodium Mordenite
沸石,HS-642粉末,Na-丝光型
品牌:Wako
品牌中文简称:和光纯药
CAS No.:N/A
Wako 322-27792 沸石,HS-642粉末,Na-丝光型

321-27762 沸石,HS-320 粉末,H-Y型 Zeolite, Synthetic, HS-320 Powder, Hydrogen Y 25g 308081-00-7
133-08645 分子筛3A 1/8 Molecular Sieves 3A 1/8 No grade 500g 308080-99-1
322-27792 沸石,HS-642粉末,Na-丝光型 Zeolite, Synthetic, HS-642 Powder, Sodium Mordenite 25g
322-27812 沸石,HS-720粉末,K-镁碱型 Zeolite, Synthetic, HS-720 Powder, Potassium Ferrierite 25g 308066-04-8
328-27772 HS-341沸石合成粉铵Y Zeolite, Synthetic, HS-341, Powder, Ammonium Y 25g
130-06075 分子筛5A 1/16 Molecular Sieves 5A 1/16 500g 69912-79-4
134-08675 分子筛 13X 1/8 Molecular Sieves? 13X 1/8 500g 63231-69-6
137-08665 分子筛5A 1/8 Molecular Sieves 5A 1/8 No grade 500g 69912-79-4
021-07195 沸石 Boiling Stone Practical Grade 500g
325-27802 沸石,HS-690粉末,H-丝光型 Zeolite, Synthetic, HS-690 Powder, Hydrogen Mordenite 25g 308068-49-7
131-13531 分子筛3A型 Molecular Sieves Pack 3A 25g×10 308080-99-1
324-27752 HS-320沸石合成粉Y钠型 Zeolite, Synthetic, HS-320, Powder, Sodium Y 25g 308081-05-2
325-27765 HS-320沸石合成粉Y氢型 Zeolite, Synthetic, HS-320, Powder, Hydrogen Y 500g 308081-00-7
325-27782 HS-500沸石合成粉Y钾型 Zeolite, Synthetic, HS-500, Powder, Potassium L 25g 329061-95-2
326-27815 HS-720沸石合成粉钾型 Zeolite, Synthetic, HS-720, Powder, Potassium Ferrierite 500g 308066-04-8
296-32651 农残前处理柱(短柱) Presep-C Agri (Short) 10pcs×5
369-08521 Acrodisc高级注射器型过滤器25mm GxF/PVDF(0.45μm) Acrodisc Premium Syringe filter with GxF/0.45um PVDF, 25mm 50pcs
365-08501 Acrodisc高级注射器型过滤器25mm PVDF(0.45μm) Acrodisc Premium Syringe filter with 0.45um PVDF, 25mm 50pcs
366-00111 针式过滤器(水系、有机系)25mm Acrodisc GHP(0.2μm) Acrodisc/25mm/PVDF/0.2um 50pcs
295-34181 滤膜用于前处理柱M型 Presep Filter for Type M 10EA
295-34201 滤膜用于前处理柱3L型,4L型 Presep Filter for Type 3L, 4L 10EA
363-00741 有机系滤膜Ekicrodisc 13CR(0.45μm) Ekicrodisc13Cr/13mm/Cr/0.45um 100pcs
296-35331 Presep(分馏用)的聚丙烯针筒型前处理柱(luer lock型)3L型 Presep (Luer Lock) Silica Gel (Inject Column) Type 3L (110g/200ml) 5EA 63231-67-4
295-48851 氨丙基硅胶前处理柱 Presep -C NH2 for Sample Pretreatment 10pcs×5
291-33603 (分馏用)的聚丙烯针筒型硅胶前处理柱(luer lock)L型(30g/70ml) (Luer Lock) Silica Gel Type L (30g/70ml) 10EA×10
291-34063 聚丙烯针筒型(Luer Lock)硅胶前处理柱L型号(35g/70ml)(Luer Lock) Silica Gel (HC-N) Type L (35g/70ml) 100EA
291-34563 (分馏用)的聚丙烯针筒型氨丙基硅胶前处理柱(luer lock)L型(34g/70ml)(Luer Lock) NH2(HC) Type L (34g/70ml) 100EA
303-95051 固相萃取柱Smart-SPE GCS-20 Smart-SPE GCS-20 100pcs
296-32151 无水硫酸钠前处理柱 Presep-C Na2SO4 for Sample 10pcs×5

日本三菱化学/MITSUBISHI CHEMICAL色谱柱

日本三菱化学/MITSUBISHI CHEMICAL色谱柱
上海金畔生物代理日本三菱化学品牌全线产品,欢迎新老客户访问三菱化学官网或者前来咨询我们获取有关三菱化学相关产品信息。
日本三菱化学/MITSUBISHI CHEMICAL色谱柱
产品名称/型号
三菱液相手性柱MCI GEL CRS10W 乳酸手性柱
MCI GEL CRS10W 和MCI GEL CRS15W乳酸专用分析柱
乳酸专用分析柱MCI GEL CRS10W 和MCI GEL CRS15W
低聚糖分离色谱柱MCI GEL CK系列阳离子交换色谱柱
提供三菱化学糖类分析HPLC柱-MCI GEL CA08F阴离子交换色谱柱
符合USP L31的离子交换柱MCI GEL CDR10
三菱化学MCI GEL CHP20P
三菱化学手性色谱柱 CRS10W乳酸
三菱化学色谱柱CRS15W
低聚糖分离色谱柱MCI GEL CK系列阳离子交换色谱柱
供应三菱化学糖类分析HPLC柱-MCI GEL CA08F阴离子交换色谱柱
供应三菱化学糖类分析HPLC柱-CK08E系列阳离子交换色谱柱

三菱化学库仑法试剂-AKX/AXI/CXU Coulometric Reagents

三菱化学库仑法试剂-AKX/AXI/CXU Coulometric Reagents
库伦法试剂
AQUAMICRON ®(6种:AXI、AX、AX01、AS、AKX、CXU、FLS)是用于库仑法的卡尔费休试剂(检测水分用试剂)。【AX01跟AX是同一种,只是包装不一样,因此属于同一种】
◆原理
如下面化学反应公式1所示,卡尔费休法使用卡尔费休试剂测定水分含量,该试剂定量地、有选择性地与水发生反应。卡尔费休试剂的组成成分是碘、二氧化硫、盐基和溶剂(酒精等)。
I2+SO2+3Base+ROH+H2O→2Base•HI+Base•HSO4R •••••• (1)
卡尔费休法里有库仑法和容量滴定法。
库仑法

往以碘化物离子、二氧化硫、盐基和溶剂(酒精等)为主要成分的电解液里添加试样。如公式2所示,通过电解氧化作用产生碘,直接与卡尔费休试剂发生反应。
2I” – 2e→I2 ·····(2)
按照法拉第定律,碘的产生与电量成正比。因此,能够从电解氧化所需的电量中求得含水量。
1mg的水=10.71库仑
如图所示,需要两种类型的库伦试剂:一种是阳极电解液,置于电解池的阳极室中;另外一种是阴极电解液,置于电解池的阴极室中。库仑试剂无需估定,而且阳极液可以重复使用。
◆特点
库伦试剂AQUAMICRON ®(6种)的特点如下文所示。
AQUAMICRON ® AX/CXU
l  对环境污染小(不含四氯化碳、氯仿)
l  应用范围广(适用于石油产品。用于气化法时也无需添加其他溶剂,可直接使用)
l  高品质、高性能(检测水分的准确度高,终点稳定性好)
l  操作简单(适用于现有的库仑法水分测定系统)
AQUAMICRON ®AS/CXU
l  应用范围广(适合用于油类、石油制品)
l  高品质、高性能(检测水分的准确度高,终点稳定性好)
l  操作简单(适用于现有的库仑法水分测定系统)
AQUAMICRON ®AKX/CXU
l  对环境污染小(不含氯仿. 甲基溶纤剂)
l  应用范围广(适用于酮类、低级羧酸类、一些醛类)
l  高品质、高性能(维持AQUAMICRON ®系列的一贯好品质,保存性良好。检测水分的准确度高,终点稳定性好)
l  操作简单(适用于现有的库仑法水分测定系统)
AQUAMICRON ®FLS
l  操作简单(AQUAMICRON ®FLS试剂是配合一液型电解池一起使用的)
l  保养简单(因为电解池没有陶瓷膜片,容易保养)
◆产品&应用
库伦试剂(AQUAMICRON ®)特别:AXI可代替AX,价格更优。

货号 品名 规格 包装 主要溶剂 用途
AQ-AXI AXI 最大水分0.15mgH2O/ml 500ml 甲醇,碳酸丙烯酯,不含氯仿和吡啶 阳极液:检测有机溶剂、无机物化学品、油类、石油产品、各种汽油等
604-07485 AX
601-07255 AS 最大水分0.15mgH2O/ml 500ml 甲醇,氯仿,含有4-二甲胺基吡啶 阳极液:检测石脑油、汽油、柴油、绝缘油等
605-07591 AKX 最大水分0.15mgH2O/ml 500ml 碳酸丙烯酯,二甘醇-甲醚,不含氯仿,含有4-二甲胺基吡啶 阳极液:检测酮类、硅油、低羟基酸、某些醛类(芳香族化合物)等
602-07501 CXU 最大水分0.6mgH2O/ml 5ml*10 甲醇,不含吡啶 阴极液:与AXI/AX/AS/AKX配合使用
632-07065/ AQ-AXI FLS/AXI 最大水分0.15mgH2O/ml 500ml/500ml 甲醇,碳酸丙烯酯,不含吡啶 适用于一液型、无玻璃型电解池:有机溶液(酒精、酯类、苯、甲苯),无机气体等

日本三菱化学卡尔费休试剂使用小知识

日本三菱化学卡尔费休试剂使用小知识

目录
如何进行电终点检测法?
如何选择容量滴定法和库仑法?
如何确认水分汽化装置的准确性?
吡啶型(旧产品)和无吡啶型的区别在哪里?
为什么抑制强碱性胺的干扰反应时,需要使用水杨酸?
为什么滴定终点后的溶剂放置一段时间后颜色发生了变化?
◆如何进行电终点检测法?
  现在市面上售卖的滴定装置(容量滴定法、库仑法)采用的是电流控制电压检测法(恒流极化电压检测法)。这个方法通过在双铂电极中输出1~30μA的恒流,检测在两极之间生成的电圧(极化电压),从而测出终点。滴定溶液中含有较多水分时,会显示300~500mV的电压(极化电压)。这个电压会因为溶剂的种类、试样不同而变动。一般来说,像烃这样的电导率低的物质则会显示高电压。
进行卡尔费休滴定法操作,接近终点时,电压会突然下降到10~50mV。容量滴定法的话,当电压持续一段时间时,可判定其为终点。市面上滴定装置的时间大概是30~60秒。库仑法的话,需要先设定终点的电压,低于此电压时可判定为终点。
◆如何选择容量滴定法和库仑法?
请根据下文的标准作出判断。
(1)活用库仑法的特征
  由于不需要卡尔费休试剂(滴定剂)的标定操作,无需滴度的管理,作为日常分析来说省了不少步骤,非常便利。
(2)通过含水量来选择
  含水量大约在1%以下的时候,请使用库仑法。
  特别是,用ppm的精度去定量ppm级含水量时,库仑法更有优势。
  当含水量超过约1%的时候,虽然也可以使用库仑法,但是由于需要取少量的试样,称量的误差将会影响分析值,所以一般都会采用容量滴定法。
(3)是否有电极反应
  苯胺类,胺类,一部分的酚类等电极反应物质不适合使用库仑法。请使用合适的脱水溶剂进行容量滴定法操作。
(4)不溶于电解液的固体物质
  由于不溶于有机溶剂的固体物质会阻塞电极和隔膜,导致电流效率低下,请不要使用库仑法。这种情况请使用合适的脱水溶剂,选择容量滴定法或水分汽化法进行操作。
◆如何确认水分测定仪的准确性?
  组装水分测定仪后,试样中汽化的水分会通过干氮气的加热管、吹管,被脱水溶剂(或电解槽中的电解液)吸收,但水分到底有没有被准确地收集呢。确认水分的收集率(回收率)有以下3种方法。
(1)使用水
   一般来说,使用水是最简单的方法。使用微量注射器(容积10μl)把5或10μl(5或10mg)的水直接注入试样管里。
(2)使用二水酒石酸钠(理论含水量15.66%)
  需要使用预先经过105℃干燥的二水酒石酸钠。
(3)使用玻璃毛细管
  操作简单,可以确认1000μg前后的收集率。玻璃毛细管常用于生物领域,容积非常准确。毛细现象使其可以简单地吸取指定容积的水。
◆吡啶型(旧产品)和无吡啶型的区别在哪里?
  无吡啶型的意思是,把作为卡尔费休试剂成分之一的盐基的吡啶换成其他胺。由于吡啶具有特异性气味,不利于操作。因此把吡啶换成高沸点的液体或固体胺,使吡啶的气味消失是无吡啶型的特点。
  除了盐基之外,无吡啶型和旧产品的成分基本一致,因此需要遵从同样的安全使用准则。现已得到证实,无吡啶型的性能和旧产品的吡啶型几乎一样。
  卡尔费休试剂的反应速度是根据滴定液的pH值、二氧化硫浓度、有机溶剂的种类、以及添加试样的量和矩阵的变换而变动的。因此不可以一言断定哪个产品的反应速度更快,这需要根据具体情况具体分析。
◆为什么抑制和强碱性胺的干扰反应时,需要使用水杨酸?
  对强碱性胺(pKa值在9以上)直接进行卡尔费休滴定法的话,会出现终点稳定性差、分析值升高等的干扰反应。为抑制这种干扰反应,需要进行酸的中和前处理。以前是使用乙酸,但是有着容易发生酯化反应、一般含有较多水分等的缺点。经研究讨论后,开始使用水杨酸并取得了良好的结果。使用水杨酸的优点如下所述。
  ①酯化反应在通常的滴定操作中不受认可。
  ②可把甲醇用作滴定溶剂,得到卡尔费休反应的良好结果。
  ③水杨酸的含水量低。
  虽然水杨酸是固体,会对操作造成不便。但是事先称量约10g水杨酸,放进滴定瓶中,接着添加50ml的脱水溶剂GEX(或者MS),搅拌溶解,即可简单地完成准备。10g的水杨酸可以测至70mmol的胺。
◆为什么滴定终点后的溶剂放置一段时间后颜色发生了变化?
  完成滴定终点之后的溶液放置一段时间后,会出现颜色变黄,再次消耗卡尔费休试剂的情况,或者相反地,变成滴定过量似的红褐色。原因是湿气从外部混入,或者是溶液中含有的碘化物离子与紫外线或氧生成游离碘。

三菱化学 602-07501 阴极液,通用CXU

三菱化学 602-07501 阴极液,通用CXU
上海金畔生物代理日本三菱化学品牌全线产品,欢迎新老客户访问三菱化学官网或者前来咨询我们获取有关三菱化学相关产品信息。

【品牌】三菱化学
【货号】602-07501
【中文名称】阴极液,通用CXU
【英文名称】AQUAMICRON CXU
【规格】5ml×10
【存放】按标签所描述
【用途】科研

三菱化学 602-07501 阴极液,通用CXU

卡尔费休试剂-库伦法/容量法试剂
AQUAMICRON® 卡尔费休试剂
blob.png
卡尔费休法使用卡尔费休试剂测定水分含量,该试剂定量地、有选择性地与水发生反应。卡尔费休试剂的组成成分是碘、二氧化硫、盐基和溶剂,如酒精。
这种方法可用于库伦及容量滴定系统。该法基于卡尔费休试剂与水反应的碘定量法,如果试样中含有能够与碘反应的物质或者某些通过碘化物氧化而产生碘的物质,结果出现误差。
即使某些物质不能直接滴定,其水分还可以用间接法加以测定,如水汽化法。
◆库伦试剂(AQUAMICRON® )特别:AXI可代替AX,价格更优。

货号 品名 规格 包装 主要溶剂 用途
621-01755 AXI 最大水分0.15mgH2O/ml 500ml 甲醇,碳酸丙烯酯,不含氯仿和吡啶 阳极液:检测有机溶剂、无机物化学品、油类、石油产品、各种汽油等
604-07485 AX
601-07255 AS 最大水分0.15mgH2O/ml 500ml 甲醇,氯仿,含有4-二甲胺基吡啶 阳极液:检测石脑油、汽油、柴油、绝缘油等
605-07591 AKX 最大水分0.15mgH2O/ml 500ml 碳酸丙烯酯,二甘醇-甲醚,不含氯仿,含有4-二甲胺基吡啶 阳极液:检测酮类、硅油、低羟基酸、某些醛类(芳香族化合物)等
602-07501 CXU 最大水分0.6mgH2O/ml 5ml*10 甲醇,不含吡啶 阴极液:与AXI/AX/AS/AKX配合使用
632-07065 FLS/AXI 最大水分0.15mgH2O/ml 500ml 甲醇,碳酸丙烯酯,不含吡啶 适用于一液型、无玻璃型电解池:有机溶液(酒精、酯类、苯、甲苯),无机气体等

◆容量滴定法试剂:SS-Z(无吡啶型)

类别 货号 品名 包装 用途 规格
滴定剂 631-03495 AQUAMICRON® Titrant SS-Z 1mg 500ml 适合于一般用途低含水量,溶剂是二甘醇-甲醚 滴定量0.7~1.2mgH2O/ml
634-03505 AQUAMICRON® Titrant SS-Z 3mg 500ml 适合于一般用途,溶剂是二甘醇-甲醚 滴定量2.5~3.5mgH2O/ml
631-03515 AQUAMICRON® Titrant SS-Z 5mg 500ml/1L 一般用途高含水量,溶剂是二甘醇-甲醚 滴定量4.5~5.5mgH2O/ml
636-03521
脱水溶剂 608-07525 AQUAMICRON® Anhydrated   Solvent GEX 500ml 【通用】有机溶剂、无机化学品、农用化学品,医药品,化肥,清洁剂,食品等 含水量0.2mgH2O/ml
635-03535 AQUAMICRON® Anhydrated   Solvent OLX 500ml 【油类用】石脑油,汽油,柴油,绝缘油等 含水量0.5mgH2O/ml
605-07535 AQUAMICRON® Anhydrated   Solvent OLⅡ 500ml 【油类.油脂类用】石脑油,汽油,柴油,重油,绝缘油,油类和脂肪(硬化油,人造黄油等)等 含水量0.3mgH2O/ml
632-03545 AQUAMICRON®
Anhydrated Solvent KTX
500ml 【酮类用】酮类,硅油,乙酸和其他低羟基酸,醛类(乙醛除外)等 含水量0.5mgH2O/ml
603-07335 AQUAMICRON® Anhydrated   Solvent SU 500ml 【糖类用】糖类,蛋白质,胶质,添加剂,动物饲料等 含水量0.2mgH2O/ml

◆SS系列(吡啶型)

类别 货号 品名 包装 用途 规格
滴定剂 608-07025 Aquamicron® 滴定剂 SS 1mg 500ml 适合于一般用途低含水量,溶剂是氯仿和吡啶 滴定量0.7~1.2mgH2O/ml
601-07015 Aquamicron® 滴定剂 SS 3mg 500ml 适合于一般用途,溶剂是氯仿和吡啶 滴定量2.5~3.5mgH2O/ml
603-07215 Aquamicron® 滴定剂 SS 10mg 500ml 一般用途高含水量,溶剂是氯仿和吡啶 滴定量8~12mgH2O/ml
脱水溶剂 600-07085 Aquamicron® 脱水溶剂 ML 500ml 【通用】有机溶剂、无机化学品、农用化学品,医药品,化肥,清洁剂,食品等 含水量0.2mgH2O/ml
604-07125 Aquamicron® 脱水溶剂 MS 500ml 【通用】有机溶剂、无机化学品、农用化学品,医药品,化肥,清洁剂,食品等,含吡啶 含水量0.2mgH2O/ml
603-07075 Aquamicron® 脱水溶剂 CM 500ml 【油类.油脂类用】石脑油,汽油,柴油,重油,绝缘油,油类和脂肪(硬化油,人造黄油等)等,含氯仿 含水量0.3mgH2O/ml
604-07245 Aquamicron® 脱水溶剂 CP 500ml 【酮类用】酮类,硅油,乙酸和其他低羟基酸,醛类(乙酰除外)等,含氯仿 含水量0.5mgH2O/ml
606-07065 Aquamicron® 脱水溶剂 PE 500ml 【酮类用】酮类,硅油,乙酸和其他低羟基酸,醛类(乙酰除外)等,含吡啶 含水量0.2mgH2O/ml
607-07095 Aquamicron® 水溶剂 FM 500ml 【糖类用】糖类,蛋白质,胶质,添加剂,动物饲料等 含水量0.2mgH2O/ml
600-07105 AQUAMICRON® 脱水溶剂  PP 500ml 【醛类】乙醛、丙醛、丁醛等,含吡啶 含水量0.2mgH2O/ml
607-07115 AQUAMICRON® 脱水溶剂ME 500ml 【用于汽化器】气态试样,氮气等 含水量0.2mgH2O/ml

 
 
水标准试剂:可溯源到NIST SRM2890,附有分析检测成绩书。
 

类别 货号 品名 包装 用途 规格
容量滴定 608-07581 Aquamicron® Water Standard 10mg 8ml*10 容量法中标定
KF试剂的滴定度
滴定量
10±0.5mg H2O/ml
600-07041 Aquamicron® Standard Water Methanol   Solution 2mg 250ml 逆滴定的滴定剂
标定滴定剂的滴定量
含水量
2.0±0.01 mg H2O/ml
库伦滴定法 639-15101 Aquamicron® Water Standard 0.1mg 5ml*10 检测库伦水分仪的精确度 滴定量
0.1±0.01mg H2O/ml
604-07561 Aquamicron® Water Standard 0.2mg 5ml*10 检测库伦水分仪的精确度 滴定量
0.2±0.01mgH2O/ml
601-07571 Aquamicron® Water Standard 1mg 5ml*10 检测库伦水分仪的精确度 滴定量
1±0.05 mgH2O/ml
609-07511 Aquamicron® Check Solution P 100ml 终点调节液,可以与AX和AKX一起使用 含水量
3.8~4.2mgH2O/ml
637-07071 Aquamicron® Solid Water Standard 10g 用于带水分汽化法
装置水分仪
含水量
3.83±0.1%

 

602-07501 阴极液,通用CXU AQUAMICRON CXU 5ml×10
603-07075 容量法溶剂,用于油类CM溶剂 AQUAMICRON Solvent CM 500ml
603-07215 容量法,一般用途,滴定剂SS 10mg,含吡啶 AQUAMICRON Titrant SS 10mg 500ml
603-07335容量法溶剂,用于糖类,无吡啶SU溶剂AQUAMICRON Solvent SU500ml
604-07125 容量法溶剂,一般用途,含吡啶MS溶剂 AQUAMICRON Solvent MS 500ml
604-07245 容量法溶剂,用于酮类CP溶剂 AQUAMICRON Solvent CP 500ml
136-12121镁标准溶液(Mg 1000)Magnesium Standard Solution (Mg 1000)100ml
136-13601 镁标准溶液(Mg 100) Magnesium Standard Solution (Mg 100) 100ml
191-12111 钠标准溶液 (Na 100) Sodium Standard Solution (Na 100) 100ml
077-02131锗标准溶液(Ge 1000)Germanium Standard Solution100ml
192-06031 硅标准溶液(Si 1000) Silicon Standard Solution 100ml 7440-21-3
250-00121钇标准溶液(Y 1000)Yttrium Standard Solution100ml
257-00131镱标准溶液(Yb 1000)Ytterbium Standard Solution (1,000ppm)100ml
192-13861 硒标准溶液(Se 1000) Selenium Standard Solution (Se 1000) 100ml
261-01431锌标准溶液 (Zn 100)Zinc Standard Solution (Zn 100)100ml
081-04651钬标准溶液(Ho 1000)Holmium Standard Solution (1,000ppm)100ml
263-00891锆标准溶液(Zr 1000)Zirconium Standard Solution100ml
264-01421锌标准溶液 (Zn 1,000)Zinc Standard Solution (Zn : 1,000mg/l)100ml
139-12111锰标准溶液(Mn 100)Manganese Standard Solution (Mn 100)100ml
033-16181钴标准溶液(Co 1000)Cobalt Standard Solution(Co 1000)100ml
033-16201铜标准溶液(Cu 1000)Copper Standard Solution (Cu 1000)100ml
144-06471镍标准溶液(Ni 100)Nickel Standard Solution (Ni 100)100ml
034-16231铜标准液(Cu 100)Copper Standard Solution (Cu 100)100ml
146-04971 铌标准溶液(Nb 1000) Niobium Standard Solution 100ml
091-03851 铁标准溶液 (Fe 100) Iron Standard Solution (Fe 100) 100ml
147-06461镍标准溶液(Ni 1000)Nickel Standard Solution (Ni 1000)100ml
149-04841钕标准溶液(Nd 1000)Neodymium Standard Solution (1,000ppm)100ml
094-03841铁标准溶液(Fe 1000)Iron Standard Solution (Fe 1000)100ml
195-08721钐标准溶液(Sm 1000)Samarium Standard Solution100ml
036-16171镉标准溶液(Cd 1000)Cadmium Standard Solution (Cd 1000)100ml
196-08751钪标准溶液(Sc 1000)Scandium Standard Solution100ml
036-17891 钙标准溶液(Ca 100) Calcium Standard Solution (Ca 100) 100ml
010-15491 锑标准溶液(Sb 1,000) Antimony Standard Solution (Sb 1,000mg/l) 100ml

日本JaICA Test kit for Potential Anti Oxidant (PAO)

日本JaICA Test kit for Potential Anti Oxidant (PAO)
日本老化制御研究所 Jaica代理
上海金畔生物作为日本 JaICA 老化中国代理商,欢迎新老客户访问日本 JaICA 老化官网或者咨询我们获取更多日本 JaICA 老化产品线价格说明书等信息。
Suitable for detection of total antioxidant capacity in serum and food extracts. For research use only.
Antioxidant assay:

Oxidative stress plays on important role in various diseases and aging. The control of oxidative stress is expected to be useful to prevent diseases and aging.Oxidative stress is caused by the imbalance between reactive oxygen species (ROS) and antioxidant defense system. For accurate assessment of oxidative stress, measurement of ROS, oxidative damage and antioxidant activity may be essential. Recently, antioxidants as functional foods which scavenge ROS attract a great deal of attention.
Principle of this assay:

In the PAO assay kit, an easy and convenient method to measure antioxidant capacity is provided. Utilizing the reduction of cupric ion (Cu++ to Cu+), antioxidant capacity of samples can be detected in 5 minutes. Samples are mixed with Cu++ Solution. Cu++ are reduced by antioxidants to form Cu+. Reduced Cu+ react with Chromatic Solution (Bathocuproine) , and can be detected by absorbance at wavelength 480 to 490 nm. Antioxidant capacity can be calculated from the Cu+ formed. PAO can detect not only hydrophilic antioxidants such as Vitamin C, glutathione, but also can detect hydrophobic antioxidants such as Vitamin E. Applicable for assessment of total antioxidants of serum, foods and beverage samples.
Specifications:

Method: Colormetric assay(detection: 480 – 492 nm)
Assay range: 21.9 – 4378 碌mol/L (cupric ion reducing power)
Format: 96 wells
Storage: Room temperature (10 – 25掳C)
Applications: Human and animal serum samples, foods and beverage samples.
Required but not provided: A micro plate reader (measuring wavelength 492 nm)
Pipettes and pipette chips
Plastic test tubes
Distilled water
NaOH, HCl solution and pH meter (Not required if standards are prepared with distilled water only).

 
Content of this kit: 试剂盒组成

Standard (Uric acid powder): 1 vial
Sample diluent: 1 bottle
Cu++ solution: 1 bottle
Stop solution: 1 bottle
Micro titer plate: 1 plate (96 wells)

Assay procedure:

1) Prepare 6 levels of standards by diluting 2mM uric acid.
2) Please prepare plastic test tubes for 6 levels of standards and each sample. Pour 390 碌L of Sample Diluent, and add 10 碌L of standards or diluted samples.
3) Pour 200 碌L of mixture to Micro titer plate. Use 200 碌L of Sample Diluent for blank well.
4) Read absorbance at 490 nm (as READ1).
5) Add 50 碌L of Cu++solution to each well, mix gently, and incubate at room temperature for 3 minutes.
6) Add 50 碌L of Stop solution, mix gently, and read absorbance at 490 nm (as READ2).
7) Please draw standard curves by plotting the difference of absorbance readings (READ2 – READ1) as vertical axis, and concentration of uric acid standards (mM) as horizontal axis. Calculate the corresponding uric acid concentration of samples. Multiply corresponding uric acid concentration (mM) of samples by 2189, to estimate antioxidant power (碌mol/L).
1mM of uric acid = 2189 碌mol/L (copper reducing power)

References

1) Oxidative imbalance and cathepsin D changes as peripheral blood biomarkers of Alzheimer disease: A pilot study
E Strafacea, P Matarresea, L Gambardella, R Vona, A Sgadari,MC Silveri, W Malorni
FEBS Letters 579, p2759-766 (2005)
2) Oxidative stress and its association with coronary artery disease and different atherogenic risk factors
C. VASSALLE, L. PETROZZI , N. BOTTO, M. G. ANDREASSI and G. C. ZUCCHELLI
Journal of Internal Medicine 256, p308-315(2004)
3) Antioxidant capacity as a reliable marker of stress in dairy calves transported by road
P Pregel, E Bollo, FT Cannizzo, B Biolatti, E Contato, and PG Biolatti
Veterinary Record 156, p53-54 (2005)
4) Vitamin E-coated dialyzers reduce oxidative stress related proteins and markers in hemodialysis ? a molecular biological approach.
LA Calo, A Naso, E Pagnin, PA Davis, M Castoro, R Corradin, P Riegler, C Cascone, W Huber and A Piccoli
Clinical Nephrology, Vol.62(5), p355-361 (2004)
5) Oxidative stress-related factors in Bartter’s and Gitelman9s syndrome: relevance for angiotensin IIsignalling.
Calo LA, Pagnin E, Davis PA, Sartori M, Semplicini A.
Nephrol Dial Transplant 18(8) p1518-1525 (2003)
6) Effect of epoetin on HO-1 mRNA level and plasma antioxidants in hemodialysis patients.
Calo LA, Stanic L, Davis PA, Pagnin E, Munaretto G, Fusaro M, Landini S, Semplicini A, Piccoli A.
Int. J Clin. Ther 41(5), p187-192 (2003)
7) Restored Antioxidant Capacity Parallels the Immunologic and Virologic Improvement in Children with Perinatal Human Immunodeficiency Virus Infection Receiving Highly Active Antiretroviral Therapy.
M Martino, F Chiarelli, M Moriondo, M Torello, C Azzari, and L Galli
Clinical Immunology 100(1),p82-86 (2001)
Product name Code Assay range Assay time Format
Test kit for Potential Anti Oxidant (PAO) KPA-050 21.9-4378 碌mol/L 5 minutes 96 wells

日本JaICA Test kit for Potential Anti Oxidant (PAO)   说明书  技术资料

Instruction manual Technical information

日本Japan Institute for the Control of Aging (JaICA)

日本Japan Institute for the Control of Aging (JaICA)

日本老化制御研究所 Jaica代理
上海金畔生物作为日本 JaICA 老化中国代理商,欢迎新老客户访问日本 JaICA 老化官网或者咨询我们获取更多日本 JaICA 老化产品线价格说明书等信息。

独特的氧化应激标记物

日本控制衰老研究所从事衰老研究,目的是促进人类健康。该研究所成功研发了检测DNA氧化损伤的试剂盒,并且在这个领域处于领先地位。该研究所目前销售很多关于氧化应激研究方面的产品。这些产品在医学,药学,生物化学和食品方面都有运用。该研究所也提供定制服务。随着步入老龄化社会,预防医学和抗衰老收到越来越多的关注。
JaICA从事试剂盒销售和定制服务。试剂盒部分,研究所研发和生产用于检测氧化应激标记物的试剂盒。单抗和ELISA试剂盒可用于检测活性氧产生的氧化损伤产物。定制服务部分,使用本研究所研发的产品和积累的经验检测尿液和血液样品,检测食品。近来预防医学、抗衰老和功能性食品的关注越来越多,因此全球医院,大学,研究所、食品企业和制药企业分析氧化应激标记物,衰老相关激素和矿物质需求也越来越多。
◆氧化应激标记物概况
 

类型 产品名称 运用 备注
  尿液 血清 组织  
DNA 氧化 8-OHdG   Check ELISA Kit 是(动物) 普遍使用的氧化应激标记物
检测时间:大概3.5小时
8-OHdG   Check ELISA Kit (High Sensitivity) 是(动物) 人血清、组织和培养细胞的更低检测限。
anti-8-Hydroxy-2′-deoxyguanisine   [8-OHdG],mAb(N45.1) 8-OHGua/8-OHG低交叉反应性
anti-Thymidine   Glycol [TG], mAb (2E8) DNA特异性氧化应激标记物
脂类氧化 Hexanoyl-Lys   [HEL] ELISA Kit n:6 多不饱和脂肪酸早期氧化的新标记物
anti-Hexanoyl-Lys   [HEL], mAb (5F12) 免疫组化,免疫印迹和ELISA
anti-Hydroxy-2-nonenal   [4-HNE], mAb(HNEJ-2) 普遍使用的脂类氧化标志物
anti-Acrolein   [ACR], mAb (5F6) 免疫组化, 免疫印迹 和 ELISA.
anti-Malondialdehyde   [MDA], mAb (1F83) 免疫组化 和 免疫印迹
anti-4-Hydroxy-2-hexenal   [4-HHE], mAb (HHE53) 免疫组化
anti-Crotonaldehyde   [CRA], mAb (82D3) 免疫组化
anti-Methylglyoxal   [MG], mAb (3C) 免疫组化
anti-7-Ketocholesterol   [7-KC], mAb (35A) 胆固醇氧化标记物
蛋白氧化 Dityrosine   [DT] ELISA Kit 蛋白氧化新型标记物。检测时间:过夜 + 1.5小时。
anti-Dityrosine   [DT], mAb (1C3) 免疫组化, 免疫印迹 和 ELISA.
anti-Dibromo-tyrosine   [DiBrY], mAb (3A5) 炎症相关的蛋白氧化标记物
抗氧化检测 Total   Antioxidant Capacity [PAO] Test Kit Food
Samples
血清、食物和酒水总抗氧化检测,能够疏水和亲水的抗氧化剂。

 

日本JaICA试剂盒产品目录

日本JaICA试剂盒产品目录
日本老化制御研究所 Jaica代理
上海金畔生物作为日本 JaICA 老化中国代理商,欢迎新老客户访问日本 JaICA 老化官网或者咨询我们获取更多日本 JaICA 老化产品线价格说明书等信息。

序号 详细名称 品牌 规格
KOG-200SE New 8-OHdG Check ELISA kit/8羟基脱氧茑苷ELISA 试剂盒 JaICA 96 wells
KOG-200TE New 8-OHdG Check ELISA Trial Package (32wells) JaICA 32 wells
KOG-HS10E Highly Sensitive 8-OHdG Check ELISA kit/高灵敏 8羟基脱氧茑苷ELISA 试剂盒 JaICA 96 wells
MOG-100P Anti 8-OHdG monoclonal antibody/8羟基脱氧茑苷 单克隆抗体 JaICA 100 micro gram
MOG-020P Anti 8-OHdG monoclonal antibody/8羟基脱氧茑苷 单克隆抗体 JaICA 20 micro gram
MHN-100P Anti 4-HNE monoclonal antibody/4羟基壬烯酸单抗 JaICA 100 micro gram
MHN-020P Anti 4-HNE monoclonal antibody/4羟基壬烯酸单抗 JaICA 20 micro gram
MBY-020P Anti Di bromo tyrosine (DiBrY) monoclonal antibody/二溴络氨酸单抗 JaICA 20 micro gram
MTG-100P Anti Tymidine Glycol (TG) monoclonal antibody/抗胸苷乙二醇单克隆抗体 JaICA 100 micro gram
MDT-020P Anti Di Tyrosine (DT) monoclonal antibody/二络氨酸抗体 JaICA 20 micro gram
MHL-021P Anti Hexanoyl-Lys(HEL) monoclonal antibody (clone 5F12)/乙酰赖氨酸单克隆抗 JaICA 20 micro gram
KHL-700E Hexanoyl-Lys(HEL) ELISA kit/乙酰赖氨酸加合物ELISA试剂盒 JaICA 96 wells
KPA-050E Test kit for Potential Anti Oxidant (PAO)/总抗氧化能力ELISA试剂盒 JaICA 96 wells
MMD-030n Anti malondialdehyde (MDA) monoclonal antibody/丙二醛单克隆 JaICA 30 micro gram
MAR-020n Anti acrolein (ACR) monoclonal antibody/炳烯醛单克隆 JaICA 20 micro gram
MAR-100n Anti acrolein (ACR) monoclonal antibody/炳烯醛单克隆 JaICA 100 micro gram
MHH-030n Anti 4-hydroxy-2-hexenal (4-HHE) monoclonal antibody/4羟基 2烯醛单抗 JaICA 30 micro gram
MCA-030n Anti crotonaldehyde (CRA) monoclonal antibody/抗巴斗醛单抗 JaICA 30 micro gram
MMG-030n Anti methyglyoxal (MG) monoclonal antibody/甲基乙二醛单抗 JaICA 30 micro gram
MKC-020n Anti 7-ketocholesterol (7-KC) monoclonal antibody/7酮基胆固醇单抗 JaICA 20 micro gram
MKC-100n Anti 7-ketocholesterol (7-KC) monoclonal antibody/7酮基胆固醇单抗 JaICA 100 micro gram
OPF-010E NEW Ultra filter unit for serum pretreatment (MW cut off 10kDa, Pall Corp.)/血清预处理超滤装置(MW切断10kDa,Pall公司) JaICA 1 unit
KDT-010E Dityrosine (DT) ELISA/双络氨酸ELISA试剂盒 JaICA  96 wells

 

日本 JaICA代理 Anti Di Tyrosine (DT) 单抗

日本 JaICA代理 Anti Di Tyrosine (DT) 单抗
日本老化制御研究所自成立以来就对抗衰老进行研究,它是在有助于健康促进的理念下创建的。结果,此研究所在开发DNA氧化损伤测试试剂盒方面取得了很大的成功,并在该领域处于了领先地位。
本研究所现在销售许多氧化应激相关产品,这些产品可应用于医学,药学,生化学,食品科学等。它还利用其独特的技术开展检测服务。随着社会老龄化的发展,预防医学与抗衰老引起了越来越多的关注,在这些领域中,我们研究所将会通过开发和提供研究产品来助于营造一个健康,长寿的社会。
产品类型:
DNA氧化检测产品;
蛋白氧化检测产品;
脂肪氧化检测产品;
抗氧化检测产品。
特性:
高专一性、高灵敏性;
不与RNA损伤产生交叉反应;
易于操作、简单快速;
无需昂贵的设备和样品预处理。
上海金畔生物作为日本 JaICA 老化中国代理商,欢迎新老客户访问日本 JaICA 老化官网或者咨询我们获取更多日本 JaICA 老化产品线价格说明书等信息。
日本老化制御研究所 Jaica代理
日本 JaICA代理 Anti Di Tyrosine (DT) 单抗
产品名称: Anti Di Tyrosine (DT) 单抗
英文名称: Anti Di Tyrosine (DT) monoclonal antibody
产品货号: MDT-020P
产品规格: 20ug
产品产地: 日本
产品商标: JaICA
 

Code Product Name  
KOG-200SE New 8-OHdG Check ELISA kit 96 wells
KOG-200TE New 8-OHdG Check ELISA Trial Package (32wells) 32 wells
KOG-HS10E Highly Sensitive 8-OHdG Check ELISA kit 96 wells
MOG-100P Anti 8-OHdG monoclonal antibody 100 micro gram
MOG-020P Anti 8-OHdG monoclonal antibody 20 micro gram
MHN-100P Anti 4-HNE monoclonal antibody 100 micro gram
MHN-020P Anti 4-HNE monoclonal antibody 20 micro gram
MBY-020P Anti Di bromo tyrosine (DiBrY) monoclonal antibody 20 micro gram
MTG-100P Anti Tymidine Glycol (TG) monoclonal antibody 100 micro gram
MDT-020P Anti Di Tyrosine (DT) monoclonal antibody 20 micro gram
MHL-021P Anti Hexanoyl-Lys(HEL) monoclonal antibody (clone 5F12) 20 micro gram
KHL-700E Hexanoyl-Lys(HEL) ELISA kit 96 wells
KPA-050 Test kit for Potential Anti Oxidant (PAO) 96 wells
MMD-030n Anti malondialdehyde (MDA) monoclonal antibody 30 micro gram
MAR-020n Anti acrolein (ACR) monoclonal antibody 20 micro gram
MAR-100n Anti acrolein (ACR) monoclonal antibody 100 micro gram
MHH-030n Anti 4-hydroxy-2-hexenal (4-HHE) monoclonal antibody 30 micro gram
MCA-030n Anti crotonaldehyde (CRA) monoclonal antibody 30 micro gram
MMG-030n Anti methyglyoxal (MG) monoclonal antibody 30 micro gram
MKC-020n Anti 7-ketocholesterol (7-KC) monoclonal antibody 20 micro gram
OPF-010E NEW Ultra filter unit for serum pretreatment (MW cut off 10kDa, Pall Corp.) 1 unit
KDT-010E Dityrosine (DT) ELISA  96 wells
OPNUCP1 Nuclease P1 from Penicillium citrinum 500 units

 

日本 JaICA代理 Dityrosine (DT) ELISA

日本 JaICA代理 Dityrosine (DT) ELISA
日本 JaICA 老化 Antibody系列产品
上海金畔生物作为日本 JaICA 老化中国代理商,欢迎新老客户访问日本 JaICA 老化官网或者咨询我们获取更多日本 JaICA 老化产品线价格说明书等信息。
日本老化制御研究所 Jaica代理
Dityrosine (DT) ELISA kit.
Suitable for urine samples. For research use only.
A novel biomarker for protein oxidation

Tyrosine is one of the major targets of protein oxidation, and until today various tyrosine derivatives such as nitrotyrosine, dityrosine and halogenated tyrosine depending on the type of free radicals. DT is a tyrosine dimer derived from tyrosyl radicals which is formed by reactive oxygen species (ROS), metal-catalyzed oxidation, ultraviolet irradiation, and peroxidases. DT have been found in atherosclerotic lesions, and lipofuscin of pyramidal neurons of aged human brains. Dityrosine is one of the specific biomarkers for protein oxidation.
Recently, dityrosine is reported to exist also in urine samples. It is expected that DT may be a novel protein oxdation marker, which is non-invasively detectable. DT ELISA kit is designed for quantitative measurement of DT especially in urine samples.
试剂盒组成:

Samples: Urine
Specifity: Specific to dityrosine (tyrosine dimer)
Assay range: 0.05 – 12 碌mol/L
Measurement: 450 nm
Quantity: 96 wells
Assay time: Overnight and 2 hours
Storage: 2 – 8 掳C. Don’t freeze.
Expiration: Printed on a package


参考文献:

1) Kato Y, Wu X, Naito M, Nomura H, Kitamoto N, Osawa T:
Immunochemical detection of protein dityrosine in atherosclerotic lesion of apo-E-deficient mice using a novel monoclonal antibody.
Biochem Biophys Res Commun. 275(1), p11-15 (2000).
2) Hattori Y, Mukaide T, Jiang L, Kotani T, Tsuda H, Mano Y, Sumigama S, Hirayama T, Nagasawa H,Kikkawa F, Toyokuni S:
Catalytic ferrous iron in amniotic fluid as a predictive marker of human maternal-fetal disorders.
J Clin Biochem Nutr 56(1),p57-63(2015) doi: 10.3164/jcbn.14-82
Product name Code Assay range Format Application
Dityrosine (DT) ELISA KDT-010E 0.05 – 12 碌mol/L 96 wells Urine

使用说明书:

Instruction manual (PDF) Technical information

相关产品:

Related products: Anti DT monoclonal antibody

日本JaICA 代理Hexanoyl-Lysine adduct(HEL) ELISA Kit

日本JaICA 代理Hexanoyl-Lysine adduct(HEL) ELISA Kit
Hexanoyl-lysine adduct (HEL) ELISA kit
Suitable for assessment of oxidative stress using urine, serum and cultured cells. For research use only.
已酰-赖氨酸(HEL)ELISA试剂盒,适用于尿液,血清和细胞培养样品氧化应激的评估。
仅供研究使用。
什么是己酰-赖氨酸?

Biomarker for early stage of lipid oxidation
Oxidative damage of lipids caused by reactive oxygen species (ROS) play an important role in some diseases, lesion of cell functions and aging. Aldehydes such as malondi-aldehyde (MDA) and 4-hydroxy-2-nonenal (4-HNE) have been reported as one of the advanced lipid peroxidation products. But recently in the earlier stage of lipid peroxidation, 13-hydroperoxyoctadecanoic acid (13-HPODE) is found to be covalently bound to proteins1). Hexanoyl-Lysine adduct (HEL) is a novel lipid hydroperoxide-modified lysine residues. HEL is formed by oxidative modification by oxidized omega-6 fatty acids such as linoleic acid or arachidonic acid. HEL may be a useful biomarker for initial stage of lipid peroxidation.Monoclonal antibodies and ELISA kit have been developped, and HEL can be detected in oxidatively modified LDL, in human atherosclerotic lesions, human urine and serum. It is also reported that HEL is formed in rat muscle during exercise, and the formation is prohibited by antioxidants such as flavonoids.
由活性氧(ROS)引起的脂质氧化损伤在一些疾病、细胞功能损害和衰老中起重要作用。醛类如丙二醛(MDA)和4-羟基-2-壬烯醛(4-HNE)已被报道为先进的脂质过氧化产物之一。但最近在脂质过氧化的早期阶段,发现13-氢过氧十八烷酸(13-HPODE)与蛋白质共价结合。己酰-赖氨酸加合物(HEL)是一种新的脂质氢过氧化物修饰的赖氨酸残基。HEL是通过氧化的omega-6脂肪酸(例如亚油酸或花生四烯酸)的氧化修饰形成。HEL可能是脂质过氧化初始阶段有用的生物标志物。现在我们已经开发了HEL单克隆抗体和ELISA试剂盒,并且可以在氧化修饰的低密度脂蛋白(LDL)、人动脉粥样硬化病变、人尿液和血清中检测HEL。另据报道,大鼠肌肉在运动期间形成HEL,并且通过抗氧化剂(如类黄酮)是禁止形成的。
己酰-赖氨酸(HEL)ELISA试剂盒

JaICA have developed HEL ELISA kit in collaboration with Dr. Toshihiko Osawa (Nagoya University) and Dr. Yoji Kato (Univerisity of Hyogo). This ELISA kit can be applied to urine, serum and cultured cells form human and animal.
JaICA公司与Toshihiko Osawa博士(名古屋大学)和Yoji Kato博士(兵库大学)合作开发了Hexanoyl-Lys(HEL)ELISA试剂盒。
该ELISA试剂盒可应用于人和动物的尿液、血清和细胞培养。

Assay principle: Competitive ELISA (detection: 450 nm)
Specifity: Specific to N-epsilon-Hexanoyl-Lysine adduct
Measuring range: 2 – 700 nmol/L
Time to answer: Over night and 2 hours.
Format: 96 wells (54 samples in single assay)
Applications: Urine, serum and cultured cells from human and animals.
Storage: Store at 2 – 8掳C (don’t freeze).
Expiry: 2 years after the day of manufacturing.
Required but not provided: 50 碌L micropipettor and pipette tips
8-channel (50-200 碌L) micropipettor and tips
8 or 12-syncronous multichannel pipet and reagent tray for multichannel pipet.
4-7 掳C incubator
Microtiter plate reader (measuring wavelength 450 nm)

技术参数
检测原理:竞争法ELISA (检测: 450 nm处);
特异性:特异于N-epsilon-己酰-赖氨酸加合物;
测定范围:2 – 700 nmol/L;
反应速度:过夜+2小时;
规格:96孔(54 samples in single assay);
应用:人和动物的尿液、血清和细胞培养物;
储存:2-8℃(避免冷冻);
保质期:2年。
Content of this kit

HEL-coated Microtiter Plate: 1 plate (96 wells)
Primary Antibody (ready to use): 1 vial
Secondary Antibody: 1 vial
Secondary Antibody Buffer: 1 vial
Chromogen (TMBZ solution): 1 vial
Chromogen Buffer: 1 vial
Washing Buffer (5X): 1 vial
Stop Solution: 1 vial
Standard solution (6 levels): 1 vial each
Plate seal: 2 sheets

试剂盒组成:
HEL-包被微孔板: 1 块板(96孔)
一抗 (即用型): 1 瓶
二抗: 1 瓶
二抗缓冲液: 1 瓶
底物溶液 (TMBZ溶液): 1 瓶
底物缓冲液: 1 瓶
洗涤液 (5X): 1 瓶
终止液: 1 瓶
标准液 (6 标准): 各1 瓶
封板膜: 2 片

Product name Code Assay range Application
Hexanoyl-Lys (HEL) ELISA KHL-700E 2-700 nmol/L Urine, serum, cultured cells and other biological materials

参考文献:
1)
Yoji Kato, Yoko Mori, Yuko Makino, Yasujiro Morimitsu, Sadayuki Hiroi, Toshitsugu Ishikawa, Toshihiko Osawa, Formation of N epsilon-(hexanonyl) lysine in protein exposed to lipid hydroperoxide. J Biol Chem 274(29), p20406-20414 (1999)
Identification of HEL, which is lysine adduct of 13-HPODE.
2)
Yoji Kato, Toshihiko Osawa: Detection of lipid hydroperoxide-derived protein modification with polyclonal antibodies. Methods in Molecular Biology, vol. 186, Oxidative stress biomarkers and antioxidant protocols, D Armstrong, Ed., Human Press Inc., NJ, USA, p37-44
Immunohistochemical detection of hexanoyl-lysine adduct.
3)
Kato Y, Miyake Y, Yamamoto K, Shimomura Y, Ochi H, Mori Y, Osawa T, Preparation of a monoclonal antibody to N(epsilon)-(Hexanonyl)lysine: application to the evaluation of protective effects of flavonoid supplementation against exercise-induced oxidative stress in rat skeletal muscle. Biochem Biophys Res Commun 274(2),p389-393(2000)
Development and characterization of anti HEL monoclonal antibody.
4)
Ryo K, Yamada H, Nakagawa Y, Tai Y, Obara K, Inoue H, Mishima K, Saito I, Possible involvement of oxidative stress in salivary gland of patients with Sjogren’s syndrome. Pathobiology 73(5), p252-260 (2006)
HEL can be detected in saliva samples from patients with Sjogren’s syndrome.
5)
Suzuki T, Kazui T, Yamamoto S, Washiyama N, Ohkura K, Ohishi K, Bashar AH, Yamashita K, Terada H, Suzuki K, Akuzawa S, Fujie M, Effect of prophylactically administered edaravone during antegrade cerebral perfusion in a canine model of old cerebral infarction. J Thorac Cardiovasc Surg 133(3),p710-716 (2007)
Application to canine serum samples.
6)
Shimizu K, Ogawa F, Akiyama Y, Muroi E, Yoshizaki A, Iwata Y, Komura K, Bae S, Sato S, Increased Serum Levels of N(epsilon)-(hexanoyl)lysine, A New Marker of Oxidative Stress, in Systemic Sclerosis. J Rheumatol. 2008 Sep 1.
Serum HEL concentration from systemic sclerosis is higher than that from healthy subjects.
7)
Tamura H, Takasaki A, Miwa I, Taniguchi K, Maekawa R, Asada H, Taketani T, Matsuoka A, Yamagata Y, Shimamura K, Morioka H, Ishikawa H, Reiter RJ, Sugino N, Oxidative stress impairs oocyte quality and melatonin protects oocytes from free radical damage and improves fertilization rate. J Pineal Res 44(3),p280-287(2008)
Intrafollicular concentration of HEL was significantly reduced by these antioxidant treatment.
8)
Sakamoto Y, Ishikawa T, Kondo Y, Yamaguchi K, Fujisawa M, The assessment of oxidative stress in infertile patients with varicocele. BJU Int 101(12), p1547-1552 (2008)
Azoospermic and oligospermic patients had a significantly higher HEL concentration in seminal plasma.
9)
Kageyama Y, Takahashi M, Nagafusa T, Torikai E, Nagano A, Etanercept reduces the oxidative stress marker levels in patients with rheumatoid arthritis. Rheumatol Int 28(3),pp245-251(2008)
Urinary HEL level was reduced at 3 and 6 months after the initial treatment with etanercept.
10)
Rummenie VT, Matsumoto Y, Dogru M, Wang Y, Hu Y, Ward SK, Igarashi A, Wakamatsu T, Ibrahim O, Goto E, Luyten G, Inoue H, Saito I, Shimazaki J, Tsubota K, Tear cytokine and ocular surface alterations following brief passive cigarette smoke exposure. Cytokine 43(2),p200-208(2008)
HEL concentration in tear was increased by brief passive exposure to cigarette smoke.
11)
K Sakai, S Kino, A Masuda, M Takeuchi, T Ochi, J Osredkar, B Rejc, K Gersak, N Ramarathnam, Y Kato;
Determination of HEL (Hexanoyl-Lysine Adduct): A Novel Biomarker for Omega-6 PUFA Oxidation.
Lipid Hydroperoxide-Derived Modification of Biomolecules: Subcellular Biochemistry 77,p61-72(2014)
An original paper for HEL ELISA development.

  • 尿8-异前列腺素(15-异前列腺素F2t)ELISA试剂盒 NWLSS™ Urinary 8-Isoprostane
  • 脂质氢过氧化物检测试剂盒 NWLSS™ Lipid Hydroperoxide(LOOH) Assay Kit
  • 8-异构前列腺素(15-异前列腺素F2t)ELISA试剂盒 NWLSS™ 8-Isoprostane (15-Iso
  • 9(±)-羟基十八碳二烯酸ELISA试剂盒 NWLSS™ 9(±)-hydroxyoctadecadienoic ac
  • 己酰赖氨酸ELISA试剂盒 NWLSS™ Hexanoyl Lysine ELISA Kit
  • 丙二醛检测试剂盒 NWLSS™ Malondialdehyde(MDA) Assay Kit
  • 抗4-羟基壬烯醛单克隆抗体 Anti 4-hydroxy-2-nonenal(4-HNE) monoclonal antibody(HNE
  • 抗4-羟基壬烯醛单克隆抗体 Anti 4-hydroxy-2-nonenal(4-HNE) monoclonal antibody(HNE

使用手册        质量控制        技术资料

Instruction manual Quality control Technical information

日本 JaICA代理 Anti HEL monoclonal antibody

日本 JaICA代理 Anti HEL monoclonal antibody
日本 JaICA 老化 Antibody系列产品
上海金畔生物作为日本 JaICA 老化中国代理商,欢迎新老客户访问日本 JaICA 老化官网或者咨询我们获取更多日本 JaICA 老化产品线价格说明书等信息。
日本老化制御研究所 Jaica代理
抗己酰-赖氨酸单克隆抗体 Anti Hexanoyl-Lysine(HEL) monoclonal antibody(5F12)
抗己酰-赖氨酸单克隆抗体 Anti Hexanoyl-Lysine(HEL) monoclonal antibody(5F12)
适用于免疫组织化学和ELISA。
Anti hexanoyl-Lysine adduct monoclonal antibody.
Suitable for immunohistocheistry and ELISA. For research use only.
Oxidative damage of lipids caused by reactive oxygen species (ROS) play an important role in some diseases, lesion of cell functions and aging. Aldehydes such as malondi-aldehyde (MDA) and 4-hydroxy-2-nonenal (4-HNE) have been reported as one of the advanced lipid peroxidation products. But recently in the earlier stage of lipid peroxidation, 13-hydroperoxyoctadecanoic acid (13-HPODE) is found to be covalently bound to proteins1). Hexanoyl-Lysine adduct (HEL) is a novel lipid hydroperoxide-modified lysine residues. HEL is formed by oxidative modification by oxidized omega-6 fatty acids such as linoleic acid or arachidonic acid. This monoclonal antibody (clone 5H4)is spesific for proteins and peptides containing HEL. Suitable for HEL detection by immunohistochemistry and western blotting.
由活性氧(ROS)引起的脂质氧化损伤在一些疾病,细胞功能损害和衰老中起重要作用。醛类(如丙二醛/MDA)和4-羟基-2-壬烯醛(4-HNE)已被报道为先进的脂质过氧化产物之一,但最近在脂质过氧化的早期阶段,发现13-hydroperoxyoctadecanoic acid(13-HPODE)与蛋白质1)共价结合。
己酰-赖氨酸加合物(HEL)是一种新的脂质氢过氧化物修饰的赖氨酸残基, HEL通过氧化的omega-6脂肪酸(例如亚油酸或花生四烯酸)氧化修饰形成。
抗己酰-赖氨酸(HEL)单克隆抗体对于含有HEL的蛋白质和肽是特异的,适用于通过免疫组织化学和免疫印迹的HEL检测。
A new biomarker for lipid peroxidation   脂质过氧化新的生物标志物


Cisplatin-treated rat kidney. 顺铂处理的大鼠肾脏
(Dr. Sugiyama, Tottori Univ.)  (Sugiyama博士)

Detection of HEL at Human atherosclerotic lesions.
(Dr. Naito)
HEL在人类动脉粥样硬化病变的检测  (Naito博士)

Clone #: 5F12
Subclass: IgG1 kappa
Antigen: N epsilon hexanoyl-Keyhole limpet hemocyanin (HEL-KLH)
Specificity: Specific to Hexanoyl-Lys adducts.
Form: Lyophilized power. Containing 10mM PBS(pH7.4), 5% sucrose, 1% BSA and 0.05% Procline 950. 100 碌g/mL of IgG.
Application: Immunohistochemistry
(Recommended antibody concentration is 2 碌g/mL on paraformaldehyde fixed tissue), and ELISA
Storage: Less than -20C

• 技术参数
克隆#:5F12;
亚基:IgG1 kappa;
抗原:N epsilon己酰-钥孔血蓝蛋白(HEL-KLH);
特异性:特异于己酰-赖氨酸加合物;
形式:冻干粉,100μg/mL(200μL),PBS(pH7.4),包含5%蔗糖,1% BSA;
应用:免疫组织化学(多聚甲醛固定的组织推荐抗体浓度为2μg/mL)和ELISA;
储存:低于-20°C
参考文献:

1) Yoji Kato, Yoko Mori, Yuko Makino, Yasujiro Morimitsu, Sadayuki Hiroi, Toshitsugu Ishikawa, Toshihiko Osawa, Formation of N epsilon-(hexanonyl) lysine in protein exposed to lipid hydroperoxide. J Biol Chem 274(29), p20406-20414 (1999)
Identification of HEL, which is lysine adduct of 13-HPODE.
2) Kato Y, Miyake Y, Yamamoto K, Shimomura Y, Ochi H, Mori Y, Osawa T, Preparation of a monoclonal antibody to N(epsilon)-(Hexanonyl)lysine: application to the evaluation of protective effects of flavonoid supplementation against exercise-induced oxidative stress in rat skeletal muscle. Biochem Biophys Res Commun 274(2),p389-393(2000)
Development and characterization of anti HEL monoclonal antibody.
3) Fukuchi Y, Miura Y, Nabeno Y, Kato Y, Osawa T, Naito M, Immunohistochemical detection of oxidative stress biomarkers, dityrosine and N(epsilon)-(hexanoyl)lysine, and C-reactive protein in rabbit atherosclerotic lesions. J Atheroscler Thromb 15(4)p185-192(2008)
Application to rabbit atherosclerotic lesions.
4) Maeda R, Noiri E, Isobe H, Homma T, Tanaka T, Negishi K, Doi K, Fujita T, Nakamura E, A water-soluble fullerene vesicle alleviates angiotensin II-induced oxidative stress in human umbilical venous endothelial cells. Hypertens Res 31(1)p141-151(2008)
Application to cultured cells (HUVECs).
5) Sango K, Yanagisawa H, Kato K, Kato N, Hirooka H, Watabe K: Differential Effects of High Glucose and Methylglyoxal on Viability and Polyol Metabolism in Immortalized Adult Mouse Schwann Cells. Open Diabetes J. 1, p1-11(2008)
Application to cultured cell from mouse.

产品信息:

Product name Code Specifications Application NOTICE
Anti HEL monoclonal antibody(clone 5F12) MHL-021P 20ug of IgG Immunohistochemistry Current product
Anti HEL monoclonal antibody(clone 5H4) MHL-020P 20ug of IgG Immunohistochemistry Discontinued

使用说明书
免疫组织化学方法
技术信息
克隆#5F12和5H4的比较

Instruction manual (PDF) Immunohistochemical procedure Technical information
Comparison of clone 5F12 and 5H4 (PDF)

日本 JaICA老化Anti 4-HNE monoclonal antibody (HNEJ-2)

日本 JaICA老化Anti 4-HNE monoclonal antibody (HNEJ-2)
日本 JaICA 老化 Antibody系列产品
上海金畔生物作为日本 JaICA 老化中国代理商,欢迎新老客户访问日本 JaICA 老化官网或者咨询我们获取更多日本 JaICA 老化产品线价格说明书等信息。
日本老化制御研究所 Jaica代理
抗4-羟基壬烯醛单克隆抗体
Anti 4-hydroxy-2-nonenal(4-HNE) monoclonal antibody(HNEJ-2)
货号:MHN-100P
品牌:JaICA
规格: 100µg
产品说明 / Product Overview:
Anti 4-HNE monoclonal antibody (HNEJ-2)

Structure of 4-hydroxy-2-nonenal
 
脂质氧化标志物
适用于免疫组织化学和免疫印迹.(仅供研究使用)
关于4-羟基壬烯醛(4-hydroxy-2-nonenal; 4-HNE)
活性氧(ROS)参与活体内的脂质过氧化,膜脂质是ROS的主要靶标之一。 ROS在过氧化过程中形成各种醛。 4-羟基-2-壬烯醛(4-HNE)是一种α,β不饱和醛,可以通过omega-6不饱和脂肪酸(例如亚油酸和花生四烯酸)的过氧化形成。 特别需要指出的是,据报道在活体内形成的4-HNE源自磷脂结合的花生四烯酸。 4-HNE可能是脂质过氧化最可靠的生物标志物之一。
大肠癌细胞的高倍视图:腺癌(箭头所示)和非肿瘤性上皮细胞(star x240)。
(S. Kondo et al. Free Radical Biology & Medicine 27 pp401-410, 1999)

技术参数
克隆号#:HNEJ-2;
免疫原:4-HNE修饰的钥孔血蓝蛋白(KLH);
亚基:小鼠IgG1(kappa);制备为腹水,并纯化硫酸铵;
性状:冻干粉(含20μg或100μg IgG);
特异性:该抗体对于用其它醛(如2-壬烯醛,2-己烯醛,1-己醛,4-羟基-2-己烯醛,甲醛或戊二醛)处理的蛋白质显示出几乎可以忽略的反应性;
应用:免疫组织化学和免疫印迹;
储存:-20°C以下5年;运输时,室温下至少可稳定保持7天。

Clone #: HNEJ-2
Immunogen: 4-HNE-modified Keyhole Limpet Hemocyanin
Subclass: Mouse IgG1(kappa)
Prepared as ascite, and ammnonium sulphate purified.
Form: Lyophilized Powder (containing 20 碌g or 100 碌g of IgG)
Specificity: This antibody show almost negligible reactivity with proteins that were treated with other aldehydes, such as 2-nonenal, 2-hexenal, 1-hexanal, 4-hydroxy-2-hexenal,formaldehyde, or glutaraldehyde.
Applications: Immunohistochemistry and western blotting.
Storage: Less than -20掳C for 5 years. When transport, stable at least 7 days at room temperature.

References

Immunohistochemistry:
T.Tanaka, Y.Nishiyama, K.Okada, K.Hirota, M.Matsui, J.Yodoi, H.Hiai, and S.Toyokuni:
Induction and nuclear translocation of thioredoxin by oxidative damage in the mouse kidney: independence of tubular necrosis and sulfhydryl depletion. Lab. Invest. 77(2), p145-155 (1997)
Western blotting:
S.Toyokuni, N.Miyake, H.Hiai, M.Hagiwara, S.Kawakishi, T.Osawa and K.Uchida:
The monclonal antibody specific for the 4-hydroxy-2-nonenal histidine adduct.
FEBS Lett. 359, p189-191 (1995)
Product name Code Content
Anti 4-HNE monoclobal antibody MHN-020P 20 ug of IgG/ vial
MHN-100P 100 ug of IgG/ vial

使用说明书
免疫组织化学操作方法
技术资料

日本 JaICA 老化Anti Acrolein (ACR) monoclonal antibody

日本 JaICA 老化Anti Acrolein (ACR) monoclonal antibody
上海金畔生物作为日本 JaICA 老化中国代理商,欢迎新老客户访问日本 JaICA 老化官网或者咨询我们获取更多日本 JaICA 老化产品线价格说明书等信息。
日本老化制御研究所 Jaica代理
抗丙烯醛单克隆抗体 Anti Acrolein(ACR) monoclonal antibody
Anti Acrolein (ACR) monoclonal antibody.
Suitable for immunohistocheistry. For research use only.
Acrolein (ACR) is a representative carcinogenic aldehyde found ubiquitously in the environment and formed endogenously through oxidation reactions, such as lipid peroxidation and myeloperoxidasecatalyzed amino acid oxidation. ACR is highly reactive aldehyde and reacts with lysine residue in protein. The reaction with ACR and lysine residue leads to the formation of numerous numbers of adducts, such as formyl-dehydropiperidino-lysine (FDP-lysine) type derivative. This antibody is specific for the ACR-modified protein, especially FDP-lysine type derivative.

Specification

Clone #: 5F6
Antigen: ACR-modified keyhole-lympet hemocyanine
Form: Frozen (100 碌g/mL antibody in 10mM PBS containing 0.1% NaN3 and 0.5% BSA). Purified by Protein-A.
Application: Immunohistochemistry.
Recommended antibody concentration is 0.5-1.0 碌g/mL on paraformaldehyde fixed tissue.
Specificity: Specific for ACR-modified protein (especially FDP-lysine type derivative)
Subclass: Mouse IgG1,kappa
Storage: Less than-20°C

抗丙烯醛单克隆抗体适用于免疫组织化学。
丙烯醛(ACR)是在环境中普遍存在并且通过氧化反应(例如脂质过氧化和髓过氧化物酶催化的氨基酸氧化)内源性形成的代表性致癌醛。 ACR是高度反应性的醛,并与蛋白质中的赖氨酸残基反应。与ACR和赖氨酸残基的反应导致大量的加合物的形成,例如formyl-dehydropiperidino-lysine(FDP-赖氨酸)类型衍生物。
该抗体对ACR修饰的蛋白,特别是FDP-赖氨酸类型衍生物具有特异性。
技术参数
克隆#:5F6;
抗原:ACR修饰的钥孔血蓝蛋白;
形式:冻结(100μg/ mL抗体在含有0.1%NaN3和0.5%BSA的10mM PBS中)。通过蛋白A纯化;
应用:免疫组织化学;
推荐抗体浓度:0.5-1.0μg/ mL对于多聚甲醛固定的组织;
特异性:特定针对ACR修饰的蛋白(特别是FDP-赖氨酸类型衍生物);
亚型:小鼠IgG1,kappa;
储存:小于-20°C

References
1)Protein-bound acrolein: Potential markers for oxidativestress. K.Uchida, M.Kanematsu, K.Sakai, T.Matsuda, N.Hattori,Y.Mizuno, D.Suzuki,T.Miyata, N.Noguchi, E.Niki,T.Osawa
Proc.Natl.Acad.Sci.USA,95,4882-4887(1998)
2)Protein-bound acrolein: A novel markers of oxidativestress in Alzheimer’s Disease. Noel Y. Calingasan, Koji Uchida, and Gary E.Gibson
Journal of Neurochemistry.72(2),751-756(1999)

Product name Code Content
Anti Acrolein monoclonal antibody MAR-020n 20 ug of IgG
MAR-100n 100 ug of IgG

日本 JaICA 老化Anti Acrolein monoclonal antibody
抗丙烯醛(ACR)单克隆抗体 使用使用说明书
技术资料

日本 JaICA 老化 Antibody系列产品

日本 JaICA 老化 Antibody系列产品
上海金畔生物作为日本 JaICA 老化中国代理商,欢迎新老客户访问日本 JaICA 老化官网或者咨询我们获取更多日本 JaICA 老化产品线价格说明书等信息。
日本老化制御研究所 Jaica代理
Anti 8-OHdG/ 8-oxo-dG/ 8-hydroxy-2'-deoxyguanosine monoclonal antibody (N45.1).

8-hydroxy-2′-deoxyguanosine (8-OHdG) is a product of oxidatively damaged DNA formed by hydroxy radical, singlet oxygen and direct photodynamic action. Anti 8-OHdG monoclonal antibody (clone N45.1) is highly specific for 8-OHdG, and is suitable for immunohistochemistry. Cross reactivity have been checked for 8-OHdG analogues (guanosine(G),7-methyl-G, 6-SH-G, 8-Bromo-G, dA, dC, dT, dI, dU, dG, O6-methyl-dG, 8-OHdA, guanine(Gua),O6-methyl-Gua, 8-OH-Gua, uric acid, urea, creatine, creatinine, 8-sulfhydryl-G, 8-OH-G).
Anti thymidine glycol (TG) monoclonal antibody

Thymidineglycol (TG) is one of the major oxidation products of DNA. Thymidine (T) can be damaged by oxidative stress such as radiation and energy metabolism. Two different pathways to form TG have been suggested. Deoxythymidine in DNA is directly oxidised by hydroxy radical, to form TG. TG can be also formed through an intermediate thymidine chlorohydrin, which is derived from hypochlorous acid (HOCl) from neutrophil myeloperoxidase. Thymidineglycol is derived from DNA, not from RNA. TG is the oxidative stress marker secific for DNA damage.
Anti dityrosine (DT) monoclonal antibody (1C3).

Tyrosine is one of the major targets of protein oxidation, and until today various tyrosine derivatives such as nitrotyrosine, dityrosine and halogenated tyrosine depending on the type of free radicals. DT is a tyrosine dimer derived from tyrosyl radicals which is formed by reactive oxygen species (ROS), metal-catalyzed oxidation, ultraviolet irradiation, and peroxidases. DT have been found in atherosclerotic lesions, and lipofuscin of pyramidal neurons of aged human brains. Dityrosine is one of the specific biomarkers for protein oxidation.
Anti dibromotyrosine monoclonal antibody (3A5).

Tyrosine is one of the main targets of protein oxidation, and it have been reported form nitrotyrosine, dityrosine and halogenated tyrosines depending on the type of free radicals. For example, hypochlorous acid (HOCl) and hypobromous acid (HOBr) are derived from neutrophil myeloperoxidase (MPO) and eosinophil peroxidase (EPO), and known to form dihalogenated tyrosines such as DiBrY, DiClY and DiIY. It is expected that DiBrY may be a novel biomarker for tissue damage which is related to inflammatory and allergic disorders.
Anti 4-hydroxy-2-nonenal (4-HNE) monoclonal antibody (HNE-J2).

During the peroxidation process, a variery of aldehydes are formed. 4-hydroxy-2-nonenal (4-HNE) is an alpha, beta unsatulated aldehyde that can be formed by peroxidation of omega-6 unsatulated fatty acids such as linoleic acid and arachidonic acid. Especially, 4-HNE formed inside living body is reported to originate from phospholipid-bound arachidonic acid. 4-HNE may be one of the most reliable biomarker of lipid peroxidation. This antibody show almost negligible reactivity with proteins that were treated with other aldehydes, such as 2-nonenal, 2-hexenal, 1-hexanal, 4-hydroxy-2-hexenal,formaldehyde, or glutaraldehyde.
Anti Hexanoyl-Lysine adduct (HEL) monoclonal antibody.

Aldehydes such as malondi-aldehyde (MDA) and 4-hydroxy-2-nonenal (4-HNE) have been reported as one of the advanced lipid peroxidation products. But recently in the earlier stage of lipid peroxidation, 13-hydroperoxyoctadecanoic acid (13-HPODE) is found to be covalently bound to proteins1). Hexanoyl-Lysine adduct (HEL) is a novel lipid hydroperoxide-modified lysine residues. HEL is formed by oxidative modification by oxidized omega-6 fatty acids such as linoleic acid or arachidonic acid. This monoclonal antibody is spesific for proteins and peptides containing HEL. Suitable for HEL detection by immunohistochemistry and western blotting.
Anti Acrolein (ACR) monoclonal antibody.

Acrolein (ACR) is a representative carcinogenic aldehyde found ubiquitously in the environment and formed endogenously through oxidation reactions, such as lipid peroxidation and myeloperoxidasecatalyzed amino acid oxidation. This antibody is specific for the ACR-modified protein, especially FDP-lysine type derivative.
Anti malondialdehyde (MDA) monoclonal antibody.

Malondialdehyde (MDA) is one of the major aldehyde derive from lipid peroxidation. MDA is highly reactive aldehyde and reacts with lysine residue in protein. The reaction with MDA and lysine residue leads to the formation of numerous numbers of adducts, such as dihydropyridine-lysine (DHP-lysine) type derivative. This monoclonal antibody is specific for the MDA-modified protein, especially DHP-lysine type derivative.
Anti 4-hydroxy-2-hexenal (4-HHE) monoclonal antibody.

4-hydroxy-2-alkenal is one of the major lipid peroxidation products, and shows many biological effects such as high toxicity to cells. Among them, 4-hydroxy-2-hexenal (HHE) is an aldehyde formed during peroxidation of n-3 fatty acids such as docosahexaenoic acid. HHE is highly reactive aldehyde and reacts with histidine residue of protein to form Michael-addition type adducts. This antibody is specific for HHE-histidine Michael adduct (HHE-His) and enable to detect HHE-His in the tissue samples.
Anti Crotonaldehyde (CRA) monoclonal antibody.

Crotonaldehyde (CRA) is a representative carcinogenic aldehyde formed endogenously through lipid peroxidation. CRA is highly reactive aldehyde and reacts with lysine residue in protein. The reaction with CRA and lysine residue leads to the formation of numerous numbers of adducts. This antibody is specific for the CRA-modified protein.
Anti methylglyoxal/ Argpyrimidine (MG) monoclonal antibody.

Methylglyoxal (MG), an endogenous metabolite that increases in diabetes and is a common intermediate in the Maillard reaction (glycation), reacts with proteins and forms advanced glycation end products (AGE). MG reacts with arginine residue in protein and forms numerous numbers of adducts, such as argpyrimidine. This antibody is specific for argpyrimidine: N-(5-hydroxy-4,6-dimethylpyrimidine-2-yl)-L-ornithine.
Anti 7-ketocholesterol (7-KC) monoclonal antibody.

Cholesterol oxidation products, especially 7-Ketocholesterol (7KC), have been the focus of much attention because they are present in human atherosclerotic plaque and display a wide range of atherogenic properties in vitro and to some extant in vivo. This antibody is specific for 7KC. This antibody is useful to detect 7KC in tissue samples. A frozen tissue sample is recommended for immunohistochemistry.
Anti nitrotryptophan (NW: 6-NO2-Trp) monoclonal antibody

Reactive nitrogen species (RNS) are formed in a variety of diseases such as atherosclerosis, inflammation, cancer and neurodegenerative disiases. Peroxinitrite (ONOO) is known as a potent biological oxidant, which is formed via very fast reaction between nitric oxide (NO) and superoxide (O2). 6-nitrotryptophan (6-NO2-Trp) is shown to be one of the most stable and major ONOO-modified amino acids, and growing evidences suggest that nitrotryptophan may play a significant role in the cellular regulation under nitrosative stress.

日本 JaICA 老化Anti Malondialdehyde(MDA) monoclonal antibody

日本 JaICA 老化Anti Malondialdehyde(MDA) monoclonal antibody
中文名称:抗丙二醛单克隆抗体
英文名称:Anti Malondialdehyde(MDA) monoclonal antibody
货号:MMD-030n
规格:30ug
Anti MDA monoclonal antibody  脂质过氧化生物标志物
适用于免疫组化(仅供研究使用)
About malondialdehyde (MDA)
Malondialdehyde (MDA) is one of the major aldehyde derive from lipid peroxidation. MDA is highly reactive aldehyde and reacts with lysine residue in protein. The reaction with MDA and lysine residue leads to the formation of numerous numbers of adducts, such as dihydropyridine-lysine (DHP-lysine) type derivative. This monoclonal antibody is specific for the MDA-modified protein, especially DHP-lysine type derivative.

关于丙二醛(MDA)
丙二醛(MDA)是衍生自脂质过氧化的主要醛之一。 MDA是高度反应性的醛,并与蛋白质中的赖氨酸残基反应。 与MDA和赖氨酸残基的反应导致众多数量的加合物的形成,例如二氢吡啶-赖氨酸(DHP-赖氨酸)型衍生物。 该单克隆抗体对MDA修饰的蛋白质,特别是DHP-赖氨酸型衍生物是特异性的。
Specifications

Clone #: 1F83
Antigen: MDA-modified keyhole-lympet hemocyanine.
Form: Frozen (100 碌g/mL antibody in 10mM PBS containing 0.1% NaN3 and 0.5% BSA). Purified by Protein-A.
Application: Immunohistochemistry.
Recommended antibody concentration is 0.5-1.0 碌g/mL on paraformaldehyde fixed tissue.
Specificity: MSpecific for MDA-modified protein (especially DHP-lysine).
Subclass: Mouse IgG2a(lambda)
Storage: Less than -20掳C


Specificity of anti MDA antibody.

Immunohistochemical detection of MDA-modified protein in atherosclerotic aorta.
Dr. N Shibata, Tokyo Women’s Medical University.
技术参数
克隆号#:1F83;
抗原:MDA修饰的钥孔血蓝蛋白(KLH);
性状:冻结(100μg/mL抗体在含有0.1%NaN3和0.5% BSA的10mM PBS中),经过蛋白A纯化;
应用:免疫组织化学。对多聚甲醛固定的组织推荐抗体浓度为0.5-1.0μg/mL;
特异性:特异性针对MDA修饰的蛋白(特别是DHP-赖氨酸)。
亚基:小鼠IgG2a(lambda)
储存:小于-20℃
其他相关产品:
·  抗丙烯醛单克隆抗体 Anti Acrolein(ACR) monoclonal antibody
·  抗丙烯醛单克隆抗体 Anti Acrolein(ACR) monoclonal antibody
·  抗己酰-赖氨酸单克隆抗体 Anti Hexanoyl-Lysine(HEL) monoclonal antibody(5F12)
·  己酰-赖氨酸加合物ELISA试剂盒 Hexanoyl-Lysine adduct(HEL) ELISA Kit
References 参考文献

1) Immunochemical detection of a lipofuscin-like fluorophore derivered from malondialdehyde and lysine.
S Yamada, S Kumazawa, T Ishii, T Nakayama, K Itakura, N Shibata, M Kobayashi, K Sakai, T Osawa and K Uchida.
J.Lipid Res. 42, p1187-1196 (2001)
2) Investigation on the Origin of Sperm DNA Fragmentation: Role of Apoptosis, Immaturity and Oxidative Stress.
Muratori M, Tamburrino L, Marchiani S, Cambi M, Olivito B, Azzari C, Forti G, Baldi E
Mol Med. 21,p109-122(2015). doi: 10.2119/molmed.2014.00158.

日本 JaICA 老化Anti Malondialdehyde(MDA) monoclonal antibody技术资料:http://www.jaica.com/e/technical_forum_nof.html#MDA
日本 JaICA 老化Anti Malondialdehyde(MDA) monoclonal antibody 说明书:
http://www.jaica.com/e/pdf/mda_ab_manual.pdf

日本 Jaica老化制御研究所代理

日本 Jaica老化制御研究所代理

日本老化制御研究所自成立以来就对抗衰老进行研究,它是在有助于健康促进的理念下创建的。结果,此研究所在开发DNA氧化损伤测试试剂盒方面取得了很大的成功,并在该领域处于了领先地位。
本研究所现在销售许多氧化应激相关产品,这些产品可应用于医学,药学,生化学,食品科学等。它还利用其独特的技术开展检测服务。随着社会老龄化的发展,预防医学与抗衰老引起了越来越多的关注,在这些领域中,我们研究所将会通过开发和提供研究产品来助于营造一个健康,长寿的社会。
 
产品类型:
1. DNA氧化检测产品;
2. 蛋白氧化检测产品;
3. 脂肪氧化检测产品;
4. 抗氧化检测产品。
 
特性:
1. 高专一性、高灵敏性;
2. 不与RNA损伤产生交叉反应;
3. 易于操作、简单快速;
4. 无需昂贵的设备和样品预处理。
日本Jaica
The Japan Institute for the Control of Aging has conducted research into control of aging since its foundation under the doctrine of contributing health promotion. As a result, the institute succeeded in developing a DNA oxidative damage measuring kit, thus taking the lead for this field.
The institute now sells many products related to oxidative stress, and such products are utilized in the fields of medicine, pharmacy, biochemistry, food sciences, etc. The institute also performs assay services utilizing its unique technologies. Preventive medicine and anti-aging are receiving increased attention with the advancement of aging society. In these areas, our Institute will contribute to realizing a healthy, long-lived society by developing and supplying research products.
Type of
marker
Item Spec. Applications Remark
Urine Serum Cells
DNA
oxidation
marker
8-OHdG
New 8-OHdG Check ELISA
(Code. KOG-200SE), ELISA: 450nm, 96 wellsHighly Sensitive 8-OHdG Check ELISA
(Code. KOG-HS10E), ELISA: 450nm, 96 wells
Anti 8-OHdG monoclonal antibody (N45.1)
(Code: MOG-020P / MOG-100P)
Assay range:
0.5 – 200 ng/mLAssay range:
0.125-10 ng/mL
20 µg /
100 µg of IgG
OKOK
OKOK
-OK
OK
Suitable for urine and animal serum. Assay time: 2.5 hours.(KOG-200SE)Suitable for human serum,tissue and cultured cells. Assay time: overnight +1.5 hours(KOG-HS10E)
Highly specific to 8-OHdG. Low cross reactivity to oxidized RNA(8-OHGua/8-OHG).
Anti Thymidine Glycol (TG) antibody
(Code: MTG-100P) Clone 2E8
100 µg of IgG OK DNA-specific oxidative stress marker. Specific to TG in DNA polymer.
Lipid
oxidation
marker
HEL (13HPODE-Lys adduct)
Hexanoyl-Lysine (HEL) ELISA kit
(Code: KHL-700E), ELISA: 450nm, 96 wellsAnti Hexanoyl-Lysine (HEL) antibody
(Code: MHL-021P) Clone 5F12
Assay range:
2 – 700 nmol/L20 µg of IgG
OK- OK- OKOK Nε-Hexanoyl-Lysine (HEL) is derived from 13-HPODE, and is a novel biomarker for early stage oxidation of n:6 polyunsaturated fatty acid. Detectable in urine, serum, tissue and cultured cells.
4-HNE
Anti 4-HNE monoclonal antibody (HNEJ2)
(Code: MHN-020P / MHN-100P)
20 µg /
100 µg of IgG
OK 4-hydroxy-2-nonenal (4-HNE) is one of the major oxidized products of fatty acids. Specific to 4-HNE-modified proteins.
MDA
Anti MDA monoclonal antibody
(Code: MMD-030n) Clone 1F83Malondialdehyde assay kit (TBARS)
(Code: KMD-008W) Colorimetric: 400-700nm scan
30 µg of IgGAssay range:
0.08 – 10 µmol/L
-OK -OK OKOK Specific to Malondialdehyde(MDA)-protein adducts such as dihydropyridine-lysine (DHP-lysine).
By utilizing 3rd derivative analysis, more reliable results than that by traditional TBARS assay.
Isoprostane
Urinary isoprostane ELISA
(Code: KIP-050) ELISA: 450nm, 96wellsSerum isoprostane ELISA
(Code: KIP-001W) ELISA: 450nm, 96wells
Assay range:
0.05 – 100 ng/mLAssay range:
0.05 – 10 ng/mL
OK- -OK -OK 15-Isoprostane F2t (also known as 8-epi-PGF2α or 8-iso-PGF2α) is produced by peroxidation of lipoproteins.
For serum or tissue samples, purification by C18/Silica Sep-Pak will be needed.
Anti Acrolein (ACR) antibody
(Code: MAR-020n / MAR-100n) Clone 5F6
20 µg /
100 µg of IgG
OK Specific to ACR modified proteins: formyl-dehydropiperidino-lysine (FDP-lysine).
Anti 4-hydroxy-2-hexenal (4-HHE) antibody
(Code: MHH-030n) Clone HHE53
30 µg of IgG OK Specific to HHE-histidine Michael adduct (HHE-His).
Anti Crotonaldehyde (CRA) antibody
(Code: MCA-030n) Clone 82D3
30 µg of IgG OK Specific to CRA-protein aduucts.
Anti Methylglyoxal (MG) antibody
(Code: MMG-030n) Clone 3C
30 µg of IgG OK Specific to MG-protein adduct (argpyrimidine).
Anti 7-ketocholesterol (7-KC) antibody
(Code: MKC-020n) Clone 35A
20 µg of IgG OK Biomarker of cholesterol oxidation. Applicable for frozen tissue only.
Mitochondria
Lipid peroxide probe (mitochondria) [NEW]
(Code: PLP-466J)
5 µg x3 OK Fluorescence probe for lipid peroxides specific to mitochondia. Suitable for cultured cells.
Lipid peroxide probe (DPPP) [NEW]
(Code: PLP-350J)
10 mg OK OK Fluorescence probe for lipid peroxides. For HPLC application.
Protein
oxidation
marker
Dityrosine (Tyr dimer)
Dityrosine (DT) ELISA [NEW]
(Code: KDT-010E), ELISA: 450nm, 96 wellsAnti Dityrosine (DT) antibody
(Code: MDT-020P) Clone 1C3
Assay range:
0.05-12 µmol/L20 µg of IgG
OK- OK- -OK New biomarker specific for protein oxidation. Assay time: overnight + 1.5hours.
Anti Dibromo-Tyrosine (DiBrY) antibody
(Code: MBY-020P) Clone 3A5
20 µg of IgG OK Inflammation-related protein oxidation marker.
NO-modification
Anti Nitrotryptophan (NW) antibody [NEW]
(Code: MNW-020P) Clone 117C
20 µg of IgG OK A novel nitrosative stress marker derived from peroxynitrite and tryptophan.
Nitrotyrosine ELISA
(Code: KNT-002W) 96 wells
2 – 1500 nmol/L OK Δ
Anti-
oxidant
assay
Total Antioxidant
Test kit for Potential Antioxidant(PAO), 96 wells
(Code: KPA-050) Colorimetric: 480-492 nmTAC Peroxyl assay,  96 wells
(Code: KPR-005W) Luminescence
Assay range:
21.9- 4378 µmol/LAssay range:
0.5~30 µmol/mL
OKOK OKOK Total antioxidant assay for serum, foods and beverages. Can detect both hydrophobic and hydrophilic antioxidants.
KPR-005W can detect peroxyl radicals(ROO·) scavenging activity.
GSH
Total Glutathione assay kit 100 tests [NEW]
(Code: KGS-419J) Colorimetric: 405-415 nm
Assay range:
5-100 µmol/L
OK The most abundant antioxidant in living cells. Applicable to a variety of samples such as plant tissues and bacteria.
Enzyme
assay
SOD
SOD activity assay kit (WST-1 method)  [NEW]
(Code: KSD-311J) Colorimetric: 450 nm, 500 testsSOD activity assay kit (Hematoxylin method)
(Code: KSD-005W) Colorimetric: 560 nm, 96 wells
Cu/Zn-SOD ELISA (Human SOD1)
(Code: KSE-040W) ELISA: 450 nm, 96 wells
Mn-SOD ELISA (Human SOD2)
(Code: KSG-025W) ELISA: 450 nm, 96 wells
ec-SOD ELISA (Human SOD3)
(Code: KSF-002W) ELISA: 450 nm, 96 wells
Assay range:
5-60 U/mL
Assay range:
0.04 – 5 ng/mL
Assay range:
25 – ng/mL
Assay range:
2 – 168 ng/mL



OKEryth-
rocyte
OK

OKOK
OK
OK
OK
Can be applied to all animals, plants and bacteria.SOD1 is mainly located in erythrocytes and tissue cells. Trace concentration of SOD1 would be detected in serum.
Mn-SOD(SOD2) is detectable in Mitochondria.
CAT
Catalase activity assay kit 96 wells
(Code: KCA-003W) UV: 240 nm
Assay range:
6-150 U/mL
Eryth-
rocyte
OK
MPO activity assay kit
(Code: KMP-008W) Colorimetric: 412 nm
96 microplate assays or 30 cubette assays
Assay range:
8- 400 U/mL
OK OK
GSH metabolism
Glutathion Peroxidase (GPx) assay kit 192 wells
(Code: KGX-005W) Colorimetric: 340 nmGlutathion Reductase (GR) assay kit 192 wells
(Code: KGR-015W) Colorimetric: 340 nm
Assay range:
5-60 mU/mLAssay range:
1.5-20 mU/mL
BloodBlood OKOK
Redox
regulation
markers
Thioredoxin
Thioredoxin I ELISA (human) 96 wells
(Code: KRX-004W) ELISA: 450 nm
Assay range:
0.4- 25 ng/mL
OK Δ
Thioredoxin reductase I ELISA (human) 96 wells
(Code: KRR-004W) ELISA: 450 nm
Assay range:
0.4- 25 ng/mL
OK Δ
Peroxiredoxin I ELISA (human) 96 wells
(Code: KPX-005W) ELISA: 450 nm
Assay range:
0.5 – 32 ng/mL
OK Δ
Peroxiredoxin III ELISA (human) 96 wells
(Code: KPZ-002W) ELISA: 450 nm
Assay range:
2 – 32 ng/mL
OK Δ
Trace
elements
assay
Fe
Iron assay kit (Ferrozine) 100 tests
(Code: CFE-005) Colorimetric: 540-580 nmIron assay kit (Nitroso-PSAP) 100 tests
(Code: CFE-010) Colorimetric: 740-760 nm
UIBC assay kit (Bathophenanthroline) 100 tests
(Code: CBC-800) Colorimetric: 540-550 nm
Assay range:
5-1,000 µg/dLAssay range:
10-1,000 µg/dL
Assay range:
10-800 µg/dL
OKOK
OKOK
OK
OKOK
Iron (Fe) is an important pro-oxidant factor which produce hydroxyl radical by fenton reaction. CFE-005 and CFE-010 can be also applied to extractes from animal tissue, plants and hair samples.Detection of unsaturated iron binding capacity(UIBC). Species independent.
Cu
Copper assay kit (3,5-DiBr-PAESA) 100 tests
(Code: CCU-400) Colorimetric: 570-590 nm
Assay range:
3-400 µg/dL
OK OK OK Can detect total (free and ceruloplasmin – bond) copper. Also applicable to extractes from tissues, plants and hair.
Zn
Zink assay kit (5-Br-PAPS)
(Code: CZN-001) Colorimetric: 550-580 nm
Assay range:
4-1,000 µg/dL
OK OK OK Can detect total (free and protein – bond) zinc. Also applicable to extractes from tissues, plants and hair.
Ca
Calcium assay kit (Chlorophosphonazo III)
(Code: CCA-020) Colorimetric: 680-700 nm, 200 testsCalcium assay kit (o-Cresolphtalein-Complexone)
(Code: CCA-030) Colorimetric: 560-590 nm, 200 tests
Assay range:
0.05-20 mg/dLAssay range:
0.2-30 mg/dL
OKOK OKOK OKOK Applicable to serum, plasma, urine, saliva, extracts from tissues, plants, hair and bone samples. Avoid EDTA containing samples.
Mg
Magnesium assay kit (Xyridil Blue-I) 100 tests
(Code: CMG-035) Colorimetric: 660 nm
Assay range:
0.05-3.5 mg/dL
OK OK OK Applicable to serum, plasma, urine, saliva, extracts from tissues, plants and hair. May be interfared by EDTA, haemolysis, anticoagulants and potassium.

Nissui 05215 氯化钠多粘菌素肉汤培养基 Salt Polymyxin Broth 100g

Nissui 05215 氯化钠多粘菌素肉汤培养基 Salt Polymyxin Broth 100g
Nissui日水培养基代理
日水制药(NISSUI)官网
欢迎访问Nissui日水官网或者咨询我们获取更多产品信息。
货期:2-3周
Nissui 05215 氯化钠多粘菌素肉汤培养基 Salt Polymyxin Broth 100g

05006 SBG Broth Base “Nissui”
05002 EEM Broth “Nissui”
05009 Selenite Cystine Broth Base “Nissui”
05132 Tetrathionate Broth (TT) “Nissui”
05130 Rappaport-Vassiliadis Broth (RV) “Nissui”
05131 Buffered Peptone Water (BPW) “Nissui”
Enterobacteria Separation
05025 SSB Agar “Nissui”
05024 SSB Agar “Nissui”
05032 SS Agar with Sucrose “Nissui”
05033 SS Agar with Sucrose “Nissui”
05021 SS Agar “Nissui”
05020 SS Agar “Nissui”
05040 DHL Agar “Nissui”
05042 BTB Lactose Agar “Nissui”
05037 MacConkey Agar “Nissui”
05103 TSI Agar “Nissui”
05140 X-SAL Agar “Nissui”
05041 MLCB Agar “Nissui”
Enterobacteria Confirmation, Differentation and Identification
05104 LIM Agar “Nissui”
05106 SIM Agar “Nissui”
05148 DNA Agar “Nissui”
05111 SC Agar “Nissui”
05107 VP-MR Medium “Nissui”
05182 ONPG Disk “Nissui”
05180 Cytochrome Oxidase Test Strip “Nissui”
06626 ID Test EB-20 “Nissui”
06615 ID Test EB-20 “Nissui” EB Plate
06616 ID Test EB-20 “Nissui” EB Broth
06628 ID Test EB-20 “Nissui” EB Reagent
06673 ID Test EB-9 “Nissui”
06671 ID Test EB-9 “Nissui” EB9 Reagent
06608 Sterilized Oil Paraffin “Nissui”
08720 Quick ID GN “Nissui”
08721 Quick ID GN “Nissui” Reagent
Vibrio parahaemolyticus, cholera
05215 Salt Polymyxin Broth “Nissui”
05206 Alkaline Peptone Water “Nissui”
05201 Vibrio Agar “Nissui”
05208 PMT Agar Base “Nissui”
05204 Thiosulfate citrate bile Saccharose Agar “Nissui”
05135 X-VP Agar “Nissui”
Non-fermentative Gram-negative rod
05220 NAC Agar “Nissui”
05221 Cetrimide Agar “Nissui”
06629 ID Test NF-18 “Nissui”
06617 ID Test NF-18 “Nissui” NF Plate
06618 ID Test NF-18 “Nissui” NF Broth
06631 ID Test NF-18 “Nissui” NF Reagent
Staphylococci
05234 Staphylococcus Medium No 110 “Nissui”
05236 Mannitol Salt Agar “Nissui”
05246 PEA Agar “Nissui”
05238 Salt Egg Yolk Agar Base “Nissui”
06637 N-ID Test SP-18 “Nissui”
06613 N-ID Test SP-18 “Nissui” SP Plate
06614 N-ID Test SP-18 “Nissui” SP Broth
06638 N-ID Test SP-18 “Nissui” SP Reagent
Haemophilus / Neisseria
06635 ID Test HN-20 Rapid “Nissui”
06640 ID Test HN-20 Rapid “Nissui” HN Reagent
Anaerobes
05420 GAM Agar “Nissui”
05426 GAM Agar, Modified “Nissui”
05433 GAM Broth, Modified “Nissui”
05422 GAM Broth “Nissui”
05460 GAM Semisolid without Dextrose “Nissui”
05424 GAM Semisolid “Nissui”
05404 CW Agar Base without KM “Nissui”
05405 CW Agar Base with KM “Nissui”
05440 Bacteroides Agar “Nissui”
05441 FM Agar, Modified “Nissui”
05450 GAM Agar with GM “Nissui”
05430 BL Agar “Nissui”
05409 Clostridia Count Agar “Nissui”
Bacillus cereus
05282 NGKG Agar Base “Nissui”
Viable bacteria
05530 Sensitivity Disk Agar-N “Nissui”
05533 Mueller-Hinton Agar-N “Nissui”
05534 Sensitivity Test Broth “Nissui”
05503 Heart Infusion Agar “Nissui”
05505 Heart Infusion Broth “Nissui”
05507 Brain Heart Infusion Agar “Nissui”
05509 Brain Heart Infusion Broth “Nissui”
05514 Nutrient Agar “Nissui”
05511 Nutrient Broth “Nissui”
05516 Trypto-Soya Agar (SCD Agar) “Nissui”
05630 Trypto-Soya Broth (SCD Broth) “Nissui”
05528 Buffered Sodium Chloride Peptone Solution (pH70) “Nissui”
05522 Dorset Egg Medium “Nissui”
05527 CLED Agar “Nissui”
05601 TGC Medium, Fluid “Nissui”
Viable bacteria
05610 TGC Medium without Indicator, Liquid “Nissui”
05629 TGC Medium without Indicator, Fluid “Nissui”
05602 Dextrose Peptone Broth “Nissui”
05618 Standard Method Agar “Nissui”
05622 Plate Count Agar with BCP “Nissui”
05625 CVT Agar “Nissui”
Ecoli and Coliform
05634 Lactose Broth “Nissui”
05638 BGLB Broth “Nissui”
05648 EC Broth “Nissui”
05636 Desoxycholate Agar “Nissui”
05644 EMB Agar “Nissui”
05631 X-GAL Agar “Nissui”
05632 XM-G Agar “Nissui”
05607 Blue Light Broth “Nissui”
05591 EC-Blue 100P “Nissui”
05593 EC-Blue 100 “Nissui”
05613 EC-Blue 10 “Nissui”
06574 EC-Blue 100 Bottle Holder “Nissui”
06516 EC-Blue 10 “Nissui” Comparator
05617 EC-Blue 100 “Nissui” Comparator
06517 EC-Blue 100 “Nissui” MPN Plate
05643 MacConkey Sorbitol Agar “Nissui”
05679 EF Agar Base “Nissui”
05649 mEC Broth “Nissui”
Fungus
05701 Sabouraud Agar “Nissui”
05702 Corn Meal Agar “Nissui”
05680 AC Broth Base “Nissui”
05703 Candida GE Agar “Nissui”
05705 Czapek Dox Agar “Nissui”
05706 Malt Agar “Nissui”
05709 Potato Dextrose Agar “Nissui”
Vitamin
05800 Lactobacilli Culture Agar “Nissui”
05801 Lactobacilli Inoculum Broth “Nissui”
05802 B12 Culture Agar “Nissui”
05803 B12 Inoculum Broth “Nissui”
05819 B12 Assay Medium (Set) “Nissui”
05815 Pyridoxine Assay Medium “Nissui”
05816 Niacin Assay Medium “Nissui”
05814 Folic Acid Assay Medium “Nissui”
05817 Pantothenate Assay Medium “Nissui”
05818 Biotin Assay Medium “Nissui”
05835 Powdered Agar “Nissui”
Prepared Media
Tube Media
05169 Nissui Tube Triple Sugar Iron Agar
05170 Nissui Tube Sulfide Indole Motility Medium
05171 Nissui Tube Lysine Indole Motility Medium
Food Stamp
06053 Food Stamp “Nissui” Desoxycholate Agar (DESO)
06052 Food Stamp “Nissui” Desoxycholate Agar (DESO)
06764 Food Stamp “Nissui” X-GAL Agar (XGAL)
06763 Food Stamp “Nissui” X-GAL Agar (XGAL)
06051 Food Stamp “Nissui” Standard Method Agar (SMA)
06050 Food Stamp “Nissui” Standard Method Agar (SMA)
06776 Food Stamp “Nissui” XM-G Agar (XM-G)
06775 Food Stamp “Nissui” XM-G Agar (XM-G)
06055 Food Stamp “Nissui” TCBS Agar (TCBS)
06054 Food Stamp “Nissui” TCBS Agar (TCBS)
06057 Food Stamp “Nissui” TGSE Agar (TGSE)
06056 Food Stamp “Nissui” TGSE Agar (TGSE)
06751 Food Stamp “Nissui” MLCB Agar (MLCB)
06750 Food Stamp “Nissui” MLCB Agar (MLCB)
06753 Food Stamp “Nissui” Cereus Agar (CERE)
06752 Food Stamp “Nissui” Cereus Agar (CERE)
06757 Food Stamp “Nissui” X-SA Agar (X-SA)
06756 Food Stamp “Nissui” X-SA Agar (X-SA)
06064 Food Stamp “Nissui” Sabouraud Agar (SABO)
06063 Food Stamp “Nissui” Sabouraud Agar (SABO)
06755 Food Stamp “Nissui”
Potato dextrose Agar with Chloramphenicol (PDA)
06754 Food Stamp “Nissui”
Potato dextrose Agar with Chloramphenicol (PDA)
06231 Food Plate X “Nissui”
Compact Dry
06740 Compact Dry “Nissui” TC
06741 Compact Dry “Nissui” TC
06744 Compact Dry “Nissui” CF
06745 Compact Dry “Nissui” CF
06742 Compact Dry “Nissui” EC
06743 Compact Dry “Nissui” EC
06746 Compact Dry “Nissui” YM
06747 Compact Dry “Nissui” YM
06748 Compact Dry “Nissui” VP
06749 Compact Dry “Nissui” VP
06729 Compact Dry “Nissui” X-SA
06730 Compact Dry “Nissui” X-SA
06732 Compact Dry “Nissui” SL
06733 Compact Dry “Nissui” SL
06727 Compact Dry “Nissui” X-BC
06728 Compact Dry “Nissui” X-BC
06738 Easy Wiping Kit “Nissui”
01553 Sterilized Diluting Solution “Nissui” 9 mL × 4
01551 Sterilized Diluting Solution “Nissui” 90 mL
06737 Opener with Sterilized Diluting Solution “Nissui”
06598 Wiping Frame “Nissui” 100
06472 Sterilized Spoid “Nissui”
Clean Stamp
06781 Clean Stamp “Nissui” MSO Agar (MSO)
06780 Clean Stamp “Nissui” MSO Agar (MSO)
06785 Clean Stamp “Nissui” SCD Agar (SCD)
06784 Clean Stamp “Nissui” SCD Agar (SCD)
06787 Clean Stamp “Nissui”
SCD Agar with Lecithin, polysorbate 80 (SCDLP)
06786 Clean Stamp “Nissui”
SCD Agar with Lecithin, polysorbate 80 (SCDLP)
06783 Clean Stamp “Nissui” Mannitol Salt Agar with Egg Yolk (MSEY)
06782 Clean Stamp “Nissui” Mannitol Salt Agar with Egg Yolk (MSEY)
06791 Clean Stamp 25 “Nissui” SCD Agar (SCD)
06790 Clean Stamp 25 “Nissui” SCD Agar (SCD)
06789 Clean Stamp “Nissui” Cetrimide Kanamycin Sulfate
Nalidixic Acid Agar (PSEU)
06793 Clean Stamp 25 “Nissui” SCDLP Agar (SCDLP)
06792 Clean Stamp 25 “Nissui” SCDLP Agar (SCDLP)
06795 Clean Stamp 25 “Nissui”
Sabouraud Agar with Chloramphenicol (CPSB)
06794 Clean Stamp 25 “Nissui”
Sabouraud Agar with Chloramphenicol (CPSB)
Food Allergen Detection Kit
08630 FASTKIT SLIM EGG “Nissui”
08631 FASTKIT SLIM MILK “Nissui”
08632 FASTKIT SLIM WHEAT “Nissui”
08633 FASTKIT SLIM BUCK WHEAT “Nissui”
08634 FASTKIT SLIM PEANUT “Nissui”
08615 FASTKIT ELISA verII EGG “Nissui”
08616 FASTKIT ELISA verII MILK “Nissui”
08617 FASTKIT ELISA verII WHEAT “Nissui”
08618 FASTKIT ELISA verII BUCK WHEAT “Nissui”
08619 FASTKIT ELISA verII PEANUT “Nissui”
08606 FASTKIT ELISA verII SOYBEAN “Nissui”
08621 FA test EIA-Crustacea “Nissui”
08622 FA test Extraction Solution “Nissui”
08623 FA test Immunochromato-Crustacea “Nissui”
Tissue Culture Medium
05900 Eagle’s MEM “Nissui”
08160 Eagle’s MEM “Nissui”
05901 Eagle’s MEM “Nissui”
05902 Eagle’s MEM “Nissui”
05904 Eagle’s MEM Amino Acids and Vitamins Medium “Nissui”
05913 Dulbecco’s PBS (-) “Nissui”
08190 Dulbecco’s PBS (-) “Nissui”
08192 Dulbecco’s PBS (-) “Nissui”
05908 Glutamine “Nissui”
05915 Dulbecco’s Modified Eagle Medium “Nissui”
05919 Dulbecco’s Modified Eagle Medium “Nissui”
05963 SFM-101 “Nissui”
05911 RPMI 1640 Medium “Nissui”
05918 RPMI 1640 Medium “Nissui”
05971 ES Medium “Nissui”
05909 Medium 199 “Nissui”
05910 Ham’s F12 Medium “Nissui”
05905 Hank’s solution “Nissui”
05906 Hank’s solution “Nissui”

Nissui日水微生物培养基产品线

品名 英文名 用途 编号 包装
AC肉汤基础培养基 AC Broth Base 肠球菌 05680 100g
溴甲酚紫平板计数培养基 Plate Count Agar with BCP 测定乳酸菌数量 05622 300g
VP培养基 BC Test VP Medium VP(Veoges-Proskauer) 05154 50支
煌绿乳糖胆盐肉汤(BGLB) BGLB Broth 大肠菌群 05638 300g
Blue light培养基(颗粒) Blue Light Broth 迅速检测大肠菌群及大肠杆菌 05607 300g
BL琼脂培养基 BL Agar 厌氧性细菌的分离、双歧杆菌的鉴别 05430 300g
CLED琼脂培养基 CLED Agar 尿中细菌的分离、定量 05527 300g
CVT琼脂培养基 CVT Agar 测定低温细菌数量 05625 100g
CW琼脂基础培养基(不含KM) CW Agar Base without KM 厌氧性细菌用 05401 100g
DNA琼脂培养基 DNA Agar DNase产生能力确认 05148 100g
GAM半固体培养基 GAM Semisolid 厌氧菌的选择分离 05424 100g
维生素B12培养琼脂 B12 Culture Agar 赖氏乳杆菌(Lactobacillus leichmannii) 05802 100g
NAC琼脂培养基 NAC Agar 非发酵性革兰氏阴性杆菌用 05520 300g
PMT琼脂基础培养基 PMT Agar Base 分离艾尔托霍乱菌 05208 240g
TGC培养基(颗粒) TGC Medium, Fluid 细菌用 05601 300g
X-GAL琼脂培养基 X-GAL Agar 05642 300g
X-SAL琼脂培养基 X-SAL Agar 沙门氏菌分离 05133 300g
标准平板计数培养基 SPC:Standard Plate Count 测定普通活菌数量 05618 300g
改良FM培养基 FM Agar, Modified 梭形杆菌的鉴别、分离 05441 100g
缓冲蛋白胨水 Buffered Peptone Water(BPW) 增加沙门氏菌前培养 05131 300g
类杆菌培养基 Bacteroides Agar 类杆菌的鉴别、分类 05440 100g
葡萄糖蛋白胨培养基 Dextrrose Peptone Broth 真菌(霉菌、酵母)用 05602 300g
氯化钠多粘菌素肉汤 Salt Polymyxin Broth 增加肠炎弧菌 05215 100g
心脏浸液琼脂培养基(颗粒) Heart Infusion Agar 一般细菌用 05503 300g
亚硒酸盐胱氨酸肉汤基础培养基 Selenite Cystine Broth Base 选择增加沙门氏菌 05009 100g
亚硒酸盐肉汤亮绿琼脂基础培养基 SBG Broth Base 选择增加沙门氏菌 05006 100g
一般乳酸菌保存检测用培养基 Lactobacilli Culture Agar 维生素定量用 05800 100g
敏感性测定的肉汤 Sensitivity Test Broth MIC测定 05534 300g
月桂醇硫酸MUG肉汤培养基 Lauryl Sulfate MUG Broth 迅速检查大肠菌群、大肠杆菌 05639 300g

Hampton HR2-643 Parabar 10312 Hampton代理

Hampton HR2-643 Parabar 10312 Hampton代理
Hampton蛋白结晶试剂耗材
Hampton Research代理
欢迎访问Hampton Research官网或者咨询我们获取更多有关蛋白结晶试剂耗材产品信息。
Parabar 10312 (previously known as Paratone)
Parabar 10312 (previously known as Paratone)
HR2-643 Parabar 10312 100 ml bottle
Parabar 10312 (previously known as Paratone)
HR2-862 Parabar 10312 5 ml bottle
Applications
Oil based cryoprotectant
Features
Synonyms used in the crystallographic literature: Paratone® N, Paratone® 8277, Infineum V8512
Oil based cryoprotectant
Description 
Parabar 10312 is a viscous cryoprotectant for both small and large molecule crystallography.1 Mildly air-unstable, small molecule compounds can be coated with Parabar under an inert atmosphere. The Parabar protected crystal sample can be cryocooled in a chilled nitrogen gas stream. Parabar has also been used successfully as a cryoprotectant for biological macromolecule crystals. Used during cryocrystallography to displace and reduce the amount of water (mother liquor, reagent) on the crystal after the crystal is mounted in a cryoloop. Coating the crystal with Parabar can minimize evaporation from the crystal and reduce exposure and slow diffusion of air (oxygen) to the crystal.
HR2-643 Parabar 10312 is supplied as 100 ml in an amber glass bottle, no applicator rod.
HR2-862 Parabar 10312 is supplied as 5 ml in an amber glass vial with glass applicator rod in screw cap lid.
The viscosity of Parabar can be reduced to improve handling with fragile needle and blade crystals by mixing 50:50 with Paraffin Oil.
Parabar is a blend of polyisobutylenes with a Staudinger molecular weight of about 20,000 and contains about 20% polymer.
Refractive index 1.48487 at 20 degrees Celsius.
Kinematic viscosity 641 cSt at 100 degrees Celsius. Kinematic viscosity ratio 2.05 at 100 degrees Celsius.
Clear to slightly hazy is appearance.
Synonyms used in the crystallographic literature: Paratone® N, Paratone® 8277, Infineum V8512
Paratone® is a registered trademark of Chevron Corporation.

CAT NO NAME DESCRIPTION
HR2-643 Parabar 10312 100 ml bottle, each
HR2-862 Parabar 10312 5 ml bottle with applicator rod, each

 

References
1. H. Hope, Cryocrystallography of biological macromolecules: a generally applicable method. Acta Cryst. (1988) B44, 22-26.
2. Structure of the ligand-binding domain (LBD) of human androgen receptor in complex with a selective modulator LGD2226. F. Wang, X.- Liu, H. Li, K.- Liang, J. N. Miner, M. Hong, E. A. Kallel, A. van Oeveren, L. Zhi and T. Jiang. Acta Cryst. (2006). F62, 1067-1071
3. H. Hope, Annu. Rev. Biophys. Chem. 1990 19:107-126
4. S. Parkin and H. Hope, J. Appl. Cryst. (1998) pages 945-953

Wako 077-03155 Gelatin 明胶

Wako 077-03155 Gelatin 明胶
英文名称:Gelatin
中文名称:明胶
品牌:Wako
品牌中文简称:和光纯药
CAS No.:9000-70-8
储存条件:室温
纯度:-
Wako 077-03155 Gelatin 明胶

品牌 产品编号 产品名称 等级 规格 CAS No.
Wako 和光纯药 071-01791 Gelatin, Capsules No. 00  明胶,胶囊No. 00 for Electron Microscopy 100 pcs N/A
Wako 和光纯药 074-01801 Gelatin, Capsules No. 0 明胶,胶囊No. 0 for Electron Microscopy 100 pcs N/A
Wako 和光纯药 190-15805 StemSure® 0.1w/v% Gelatin Solution StemSure 0.1w/v%明胶溶液 for Cell Culture 500 ml
Wako 和光纯药 071-06291 Gelatin, from Bovine Bone 明胶,来自牛骨 100 g 9000-70-8
Wako 和光纯药 073-06295 Gelatin, from Bovine Bone 明胶,来自牛骨 500 g 9000-70-8
Wako 和光纯药 077-03155 Gelatin 明胶 Wako 1st Grade 500 g 9000-70-8
Nippi 日皮 305-34331 gelatin, M.W.8,000±2,000, High Grade  明胶,M.W.8,000±2,000, 高纯 10 g 9015-54-7
Nippi 303-34413 gelatin, M.W.100,000 明胶,分子量100,000 1 g
Nippi 301-34333 gelatin, M.W.8,000±2,000, High Grade 明胶,分子量8,000±2,000 1 g 9015-54-7
Nippi 301-34414 gelatin, M.W.100,000 明胶,分子量100,000 5 g
Nippi 308-34343 gelatin, M.W.60,000, High Grade 明胶,分子量60,000 10 g
Hokudo 301-13931 Gelatinase A 明胶酶A 10 ug
Hokudo 308-13941 Gelatinase B 明胶酶B 10 ug
Hokudo 302-13841 Anti Gelatinase B Polyclonal Antibody 抗明胶酶A多克隆抗体 100 ul

Wako 016-17431 消泡剂SI

Wako 016-17431 消泡剂SI
英文名称:Antifoam SI
中文名称:消泡剂SI
品牌:Wako
品牌中文简称:和光纯药
CAS No.:N/A
储存条件:25℃以下
纯度:-
细胞培养消泡剂
消泡剂 SI,PE-H,PE-M,PE-L
特点:

  • SI(Silicone)

本品为硅系消泡剂,同时具有非常优异的破泡性和抑泡性。因此,低浓度时,也可得到消泡效果。而且,添加到培养基时,具有浑浊少的特点。

  • PE-H(Pdyether-High), PE-M(Polyether-Medium), PE-L(Polyether-Low)

本品为聚醚系消泡剂,可将细胞毒性抑制到非常低的程度。根据粘度的不同,分为PE-H,PE-M,PE-L三种。一般来说,粘度越低分散性越好。
添加量:对于细胞培养,添加适量本品用于确认各系统的最终浓度保持在0.25~0.5%(1L培养基中含0.25~0.5ml)。
用途: 用于细菌·细胞等培养时去除泡沫.
参考数据:1.消泡效果和浑浊(度)的测定.
方法:在小试验管中注入5ml培养基,剧烈振荡后,加入消泡剂。
添加后分别测定10秒钟、5分钟、15分钟的起泡程度,以及当时的浑浊(度)。
2.细胞毒性试验.
细胞:Vero细胞的subclone(O78株)
Viability的判定:Alamar Blue.
方法:添加Alamar Blue, 测定LD50.
注意:细胞毒性非常低,用于培养没有问题。可用于各系统的培养。

产品编号 产品名称 包装
016-17431 Antifoam SI   消泡剂 SI 100ml
018-17435 Antifoam SI   消泡剂 SI 500ml
010-17191 Antifoam PE-H 消泡剂 PE-H (参考)在20℃时,粘度约983cP 100ml
012-17195 Antifoam PE-H 消泡剂 PE-H (参考)在20℃时,粘度约983cP 500ml
010-17211 Antifoam PE-M 消泡剂 PE-M (参考)在20℃时,粘度约325cP 100ml
012-17215 Antifoam PE-M 消泡剂 PE-M (参考)在20℃时,粘度约325cP 500ml
013-17201 Antifoam PE-L 消泡剂 PE-L (参考)在20℃时,粘度约160cP 100ml
015-17205 Antifoam PE-L 消泡剂 PE-L (参考)在20℃时,粘度约160cP

WAKO 122-02651 脂肪酶 Lipase 9001-62-1

WAKO 122-02651 脂肪酶 Lipase 9001-62-1

基本信息

中文名称:脂肪酶
中文别名:脂肪酶;脂肪水解酶;脂酶;胰脂肪酶;脂及酶
英文名称:Lipase
英文别名:Lipase AS Amano;Lipase AK Amano;Lipase Ays Amano;Lipase Cal-B;Lipase Mml;Lipase PS Amano

基本属性

外观:近白色冻干粉末或乳黄色粉末,溶于水,最适温度35~370C.

产品说明

储存条件:2-8°C

WAKO 122-02651 脂肪酶 Lipase 9001-62-1

Wako 和光纯药 326-91202 Lipase PS IM Amano,Immobilized on Diatomaceous Earth 脂肪酶PS IM“天野”,固定化于硅藻土 25 g
Wako 和光纯药 122-02651 Lipase 脂肪酶 for Biochemistry 100 mg 9001-62-1
Wako 和光纯药 328-91201 Lipase PS IM Amano,Immobilized on Diatomaceous Earth 脂肪酶PS IM“天野”,固定化于硅藻土 5 g
Wako 和光纯药 129-04501 Lipoprotein Lipase 脂蛋白脂肪酶 for Biochemistry 250 mg (9004-02-8)
Wako 和光纯药 121-06543 Lipase AK Amano 脂肪酶AK“天野” 50g 9001-62-1
Wako 和光纯药 124-06533 Lipase PS Amano SD 脂肪酶PS天野SD for Organic Synthesis 50 g 9001-62-1
Wako 和光纯药 128-06531 Lipase PS Amano SD 脂肪酶PS天野SD for Organic Synthesis 10 g 9001-62-1
Wako 和光纯药 323-58391 Lipase AK Amano 脂肪酶AK“天野” 10 g 9001-62-1
Wako 和光纯药 329-58393 Lipase AK Amano 脂肪酶AK“天野” 50 g 9001-62-1
Wako 和光纯药 127-06501 Lipase PS IM Amano, Immobilized on Diatomaceous Earth Amano 脂肪酶 PS-IM(硅藻土载体固定) For Organic Synthesis 5 g
Wako 和光纯药 321-58353 Lipase F-AP15 脂肪酶F-AP15 50 g 9001-62-1
Wako 和光纯药 321-88523 Lipase PS Amano SD 脂肪酶PS”天野”SD 50 g 9001-62-1
Wako 和光纯药 125-06502 Lipase PS IM Amano, Immobilized on Diatomaceous Earth Amano 脂肪酶 PS-IM(硅藻土载体固定) for Organic Synthesis 25 g
Wako 和光纯药 125-06541 Lipase AK Amano 脂肪酶AK“天野” 10g 9001-62-1
Wako 和光纯药 325-58373 Lipase AYS Amano 脂肪酶AYS“天野” 50 g 9001-62-1