日本关东化学Kanto 氯化钙 CAS 10035-04-8

日本关东化学Kanto 氯化钙 CAS 10035-04-8
日本关东化学代理商–上海金畔生物
代理优势:货期2-3周。
欢迎访问关东化学株式会社Kanto官网或者咨询我们获取更多产品信息。
日本关东化学Kanto 氯化钙 CAS 10035-04-8

Product No. 07058-00
Product name Calcium chloride dihydrate
Grade Guaranteed reagent for JIS
Purity 99.0%~103.0%(T)
Package 500g
Price ¥2,000
Stock 100<=
Product information   JISK8122

日本关东化学Kanto 氯化钙 CAS 10035-04-8

Product No. Product Name Grade Package Stock
07058-00 Calcium chloride dihydrate Guaranteed reagent for JIS 500g 100<=
07058-01 Calcium chloride dihydrate Extra pure 500g 100<=
07058-23 Calcium chloride dihydrate for molecular biology 100g 50<=
07058-80 Calcium chloride dihydrate Guaranteed reagent for JIS 15kg 50<=
07058-81 Calcium chloride dihydrate Extra pure 15kg 50<=
2382-1M Calcium chloride dihydrate EMSURER 250g non-stocked
58001-17 Calcium chloride 0‐2H2O Food additives 500g 100<=
58001-41 Calcium chloride 0‐2H2O Food additives 20kg Contact us
42352-1A Calcium chloride dihydrate   25g non-stocked
42352-2A Calcium chloride dihydrate   500g non-stocked

日本Kanto关东化学 37280-00 硫酸钠

日本Kanto关东化学 37280-00 硫酸钠
日本关东化学代理商–上海金畔生物
代理优势:货期2-3周。
欢迎访问关东化学株式会社Kanto官网或者咨询我们获取更多产品信息。
日本Kanto关东化学 37280-00 硫酸钠

Product No. 37280-00
Product name Sodium sulfate
Grade Guaranteed reagent for JIS
Purity >99.0%(T)
Package 500g
Price ¥1,300
Stock 100<=
Product information JISK8987
Formula Na2SO4
Formula weight 142.04
Physical properties mp 884℃
(△/Specification ▲/Average result †/Litureture)
CAS No. 7757-82-6

日本JaICA Test kit for Potential Anti Oxidant (PAO)

日本JaICA Test kit for Potential Anti Oxidant (PAO)
日本老化制御研究所 Jaica代理
上海金畔生物作为日本 JaICA 老化中国代理商,欢迎新老客户访问日本 JaICA 老化官网或者咨询我们获取更多日本 JaICA 老化产品线价格说明书等信息。
Suitable for detection of total antioxidant capacity in serum and food extracts. For research use only.
Antioxidant assay:

Oxidative stress plays on important role in various diseases and aging. The control of oxidative stress is expected to be useful to prevent diseases and aging.Oxidative stress is caused by the imbalance between reactive oxygen species (ROS) and antioxidant defense system. For accurate assessment of oxidative stress, measurement of ROS, oxidative damage and antioxidant activity may be essential. Recently, antioxidants as functional foods which scavenge ROS attract a great deal of attention.
Principle of this assay:

In the PAO assay kit, an easy and convenient method to measure antioxidant capacity is provided. Utilizing the reduction of cupric ion (Cu++ to Cu+), antioxidant capacity of samples can be detected in 5 minutes. Samples are mixed with Cu++ Solution. Cu++ are reduced by antioxidants to form Cu+. Reduced Cu+ react with Chromatic Solution (Bathocuproine) , and can be detected by absorbance at wavelength 480 to 490 nm. Antioxidant capacity can be calculated from the Cu+ formed. PAO can detect not only hydrophilic antioxidants such as Vitamin C, glutathione, but also can detect hydrophobic antioxidants such as Vitamin E. Applicable for assessment of total antioxidants of serum, foods and beverage samples.
Specifications:

Method: Colormetric assay(detection: 480 – 492 nm)
Assay range: 21.9 – 4378 碌mol/L (cupric ion reducing power)
Format: 96 wells
Storage: Room temperature (10 – 25掳C)
Applications: Human and animal serum samples, foods and beverage samples.
Required but not provided: A micro plate reader (measuring wavelength 492 nm)
Pipettes and pipette chips
Plastic test tubes
Distilled water
NaOH, HCl solution and pH meter (Not required if standards are prepared with distilled water only).

 
Content of this kit: 试剂盒组成

Standard (Uric acid powder): 1 vial
Sample diluent: 1 bottle
Cu++ solution: 1 bottle
Stop solution: 1 bottle
Micro titer plate: 1 plate (96 wells)

Assay procedure:

1) Prepare 6 levels of standards by diluting 2mM uric acid.
2) Please prepare plastic test tubes for 6 levels of standards and each sample. Pour 390 碌L of Sample Diluent, and add 10 碌L of standards or diluted samples.
3) Pour 200 碌L of mixture to Micro titer plate. Use 200 碌L of Sample Diluent for blank well.
4) Read absorbance at 490 nm (as READ1).
5) Add 50 碌L of Cu++solution to each well, mix gently, and incubate at room temperature for 3 minutes.
6) Add 50 碌L of Stop solution, mix gently, and read absorbance at 490 nm (as READ2).
7) Please draw standard curves by plotting the difference of absorbance readings (READ2 – READ1) as vertical axis, and concentration of uric acid standards (mM) as horizontal axis. Calculate the corresponding uric acid concentration of samples. Multiply corresponding uric acid concentration (mM) of samples by 2189, to estimate antioxidant power (碌mol/L).
1mM of uric acid = 2189 碌mol/L (copper reducing power)

References

1) Oxidative imbalance and cathepsin D changes as peripheral blood biomarkers of Alzheimer disease: A pilot study
E Strafacea, P Matarresea, L Gambardella, R Vona, A Sgadari,MC Silveri, W Malorni
FEBS Letters 579, p2759-766 (2005)
2) Oxidative stress and its association with coronary artery disease and different atherogenic risk factors
C. VASSALLE, L. PETROZZI , N. BOTTO, M. G. ANDREASSI and G. C. ZUCCHELLI
Journal of Internal Medicine 256, p308-315(2004)
3) Antioxidant capacity as a reliable marker of stress in dairy calves transported by road
P Pregel, E Bollo, FT Cannizzo, B Biolatti, E Contato, and PG Biolatti
Veterinary Record 156, p53-54 (2005)
4) Vitamin E-coated dialyzers reduce oxidative stress related proteins and markers in hemodialysis ? a molecular biological approach.
LA Calo, A Naso, E Pagnin, PA Davis, M Castoro, R Corradin, P Riegler, C Cascone, W Huber and A Piccoli
Clinical Nephrology, Vol.62(5), p355-361 (2004)
5) Oxidative stress-related factors in Bartter’s and Gitelman9s syndrome: relevance for angiotensin IIsignalling.
Calo LA, Pagnin E, Davis PA, Sartori M, Semplicini A.
Nephrol Dial Transplant 18(8) p1518-1525 (2003)
6) Effect of epoetin on HO-1 mRNA level and plasma antioxidants in hemodialysis patients.
Calo LA, Stanic L, Davis PA, Pagnin E, Munaretto G, Fusaro M, Landini S, Semplicini A, Piccoli A.
Int. J Clin. Ther 41(5), p187-192 (2003)
7) Restored Antioxidant Capacity Parallels the Immunologic and Virologic Improvement in Children with Perinatal Human Immunodeficiency Virus Infection Receiving Highly Active Antiretroviral Therapy.
M Martino, F Chiarelli, M Moriondo, M Torello, C Azzari, and L Galli
Clinical Immunology 100(1),p82-86 (2001)
Product name Code Assay range Assay time Format
Test kit for Potential Anti Oxidant (PAO) KPA-050 21.9-4378 碌mol/L 5 minutes 96 wells

日本JaICA Test kit for Potential Anti Oxidant (PAO)   说明书  技术资料

Instruction manual Technical information

日本 JaICA代理 Anti Di Tyrosine (DT) 单抗

日本 JaICA代理 Anti Di Tyrosine (DT) 单抗
日本老化制御研究所自成立以来就对抗衰老进行研究,它是在有助于健康促进的理念下创建的。结果,此研究所在开发DNA氧化损伤测试试剂盒方面取得了很大的成功,并在该领域处于了领先地位。
本研究所现在销售许多氧化应激相关产品,这些产品可应用于医学,药学,生化学,食品科学等。它还利用其独特的技术开展检测服务。随着社会老龄化的发展,预防医学与抗衰老引起了越来越多的关注,在这些领域中,我们研究所将会通过开发和提供研究产品来助于营造一个健康,长寿的社会。
产品类型:
DNA氧化检测产品;
蛋白氧化检测产品;
脂肪氧化检测产品;
抗氧化检测产品。
特性:
高专一性、高灵敏性;
不与RNA损伤产生交叉反应;
易于操作、简单快速;
无需昂贵的设备和样品预处理。
上海金畔生物作为日本 JaICA 老化中国代理商,欢迎新老客户访问日本 JaICA 老化官网或者咨询我们获取更多日本 JaICA 老化产品线价格说明书等信息。
日本老化制御研究所 Jaica代理
日本 JaICA代理 Anti Di Tyrosine (DT) 单抗
产品名称: Anti Di Tyrosine (DT) 单抗
英文名称: Anti Di Tyrosine (DT) monoclonal antibody
产品货号: MDT-020P
产品规格: 20ug
产品产地: 日本
产品商标: JaICA
 

Code Product Name  
KOG-200SE New 8-OHdG Check ELISA kit 96 wells
KOG-200TE New 8-OHdG Check ELISA Trial Package (32wells) 32 wells
KOG-HS10E Highly Sensitive 8-OHdG Check ELISA kit 96 wells
MOG-100P Anti 8-OHdG monoclonal antibody 100 micro gram
MOG-020P Anti 8-OHdG monoclonal antibody 20 micro gram
MHN-100P Anti 4-HNE monoclonal antibody 100 micro gram
MHN-020P Anti 4-HNE monoclonal antibody 20 micro gram
MBY-020P Anti Di bromo tyrosine (DiBrY) monoclonal antibody 20 micro gram
MTG-100P Anti Tymidine Glycol (TG) monoclonal antibody 100 micro gram
MDT-020P Anti Di Tyrosine (DT) monoclonal antibody 20 micro gram
MHL-021P Anti Hexanoyl-Lys(HEL) monoclonal antibody (clone 5F12) 20 micro gram
KHL-700E Hexanoyl-Lys(HEL) ELISA kit 96 wells
KPA-050 Test kit for Potential Anti Oxidant (PAO) 96 wells
MMD-030n Anti malondialdehyde (MDA) monoclonal antibody 30 micro gram
MAR-020n Anti acrolein (ACR) monoclonal antibody 20 micro gram
MAR-100n Anti acrolein (ACR) monoclonal antibody 100 micro gram
MHH-030n Anti 4-hydroxy-2-hexenal (4-HHE) monoclonal antibody 30 micro gram
MCA-030n Anti crotonaldehyde (CRA) monoclonal antibody 30 micro gram
MMG-030n Anti methyglyoxal (MG) monoclonal antibody 30 micro gram
MKC-020n Anti 7-ketocholesterol (7-KC) monoclonal antibody 20 micro gram
OPF-010E NEW Ultra filter unit for serum pretreatment (MW cut off 10kDa, Pall Corp.) 1 unit
KDT-010E Dityrosine (DT) ELISA  96 wells
OPNUCP1 Nuclease P1 from Penicillium citrinum 500 units

 

日本 JaICA代理 Anti HEL monoclonal antibody

日本 JaICA代理 Anti HEL monoclonal antibody
日本 JaICA 老化 Antibody系列产品
上海金畔生物作为日本 JaICA 老化中国代理商,欢迎新老客户访问日本 JaICA 老化官网或者咨询我们获取更多日本 JaICA 老化产品线价格说明书等信息。
日本老化制御研究所 Jaica代理
抗己酰-赖氨酸单克隆抗体 Anti Hexanoyl-Lysine(HEL) monoclonal antibody(5F12)
抗己酰-赖氨酸单克隆抗体 Anti Hexanoyl-Lysine(HEL) monoclonal antibody(5F12)
适用于免疫组织化学和ELISA。
Anti hexanoyl-Lysine adduct monoclonal antibody.
Suitable for immunohistocheistry and ELISA. For research use only.
Oxidative damage of lipids caused by reactive oxygen species (ROS) play an important role in some diseases, lesion of cell functions and aging. Aldehydes such as malondi-aldehyde (MDA) and 4-hydroxy-2-nonenal (4-HNE) have been reported as one of the advanced lipid peroxidation products. But recently in the earlier stage of lipid peroxidation, 13-hydroperoxyoctadecanoic acid (13-HPODE) is found to be covalently bound to proteins1). Hexanoyl-Lysine adduct (HEL) is a novel lipid hydroperoxide-modified lysine residues. HEL is formed by oxidative modification by oxidized omega-6 fatty acids such as linoleic acid or arachidonic acid. This monoclonal antibody (clone 5H4)is spesific for proteins and peptides containing HEL. Suitable for HEL detection by immunohistochemistry and western blotting.
由活性氧(ROS)引起的脂质氧化损伤在一些疾病,细胞功能损害和衰老中起重要作用。醛类(如丙二醛/MDA)和4-羟基-2-壬烯醛(4-HNE)已被报道为先进的脂质过氧化产物之一,但最近在脂质过氧化的早期阶段,发现13-hydroperoxyoctadecanoic acid(13-HPODE)与蛋白质1)共价结合。
己酰-赖氨酸加合物(HEL)是一种新的脂质氢过氧化物修饰的赖氨酸残基, HEL通过氧化的omega-6脂肪酸(例如亚油酸或花生四烯酸)氧化修饰形成。
抗己酰-赖氨酸(HEL)单克隆抗体对于含有HEL的蛋白质和肽是特异的,适用于通过免疫组织化学和免疫印迹的HEL检测。
A new biomarker for lipid peroxidation   脂质过氧化新的生物标志物


Cisplatin-treated rat kidney. 顺铂处理的大鼠肾脏
(Dr. Sugiyama, Tottori Univ.)  (Sugiyama博士)

Detection of HEL at Human atherosclerotic lesions.
(Dr. Naito)
HEL在人类动脉粥样硬化病变的检测  (Naito博士)

Clone #: 5F12
Subclass: IgG1 kappa
Antigen: N epsilon hexanoyl-Keyhole limpet hemocyanin (HEL-KLH)
Specificity: Specific to Hexanoyl-Lys adducts.
Form: Lyophilized power. Containing 10mM PBS(pH7.4), 5% sucrose, 1% BSA and 0.05% Procline 950. 100 碌g/mL of IgG.
Application: Immunohistochemistry
(Recommended antibody concentration is 2 碌g/mL on paraformaldehyde fixed tissue), and ELISA
Storage: Less than -20C

• 技术参数
克隆#:5F12;
亚基:IgG1 kappa;
抗原:N epsilon己酰-钥孔血蓝蛋白(HEL-KLH);
特异性:特异于己酰-赖氨酸加合物;
形式:冻干粉,100μg/mL(200μL),PBS(pH7.4),包含5%蔗糖,1% BSA;
应用:免疫组织化学(多聚甲醛固定的组织推荐抗体浓度为2μg/mL)和ELISA;
储存:低于-20°C
参考文献:

1) Yoji Kato, Yoko Mori, Yuko Makino, Yasujiro Morimitsu, Sadayuki Hiroi, Toshitsugu Ishikawa, Toshihiko Osawa, Formation of N epsilon-(hexanonyl) lysine in protein exposed to lipid hydroperoxide. J Biol Chem 274(29), p20406-20414 (1999)
Identification of HEL, which is lysine adduct of 13-HPODE.
2) Kato Y, Miyake Y, Yamamoto K, Shimomura Y, Ochi H, Mori Y, Osawa T, Preparation of a monoclonal antibody to N(epsilon)-(Hexanonyl)lysine: application to the evaluation of protective effects of flavonoid supplementation against exercise-induced oxidative stress in rat skeletal muscle. Biochem Biophys Res Commun 274(2),p389-393(2000)
Development and characterization of anti HEL monoclonal antibody.
3) Fukuchi Y, Miura Y, Nabeno Y, Kato Y, Osawa T, Naito M, Immunohistochemical detection of oxidative stress biomarkers, dityrosine and N(epsilon)-(hexanoyl)lysine, and C-reactive protein in rabbit atherosclerotic lesions. J Atheroscler Thromb 15(4)p185-192(2008)
Application to rabbit atherosclerotic lesions.
4) Maeda R, Noiri E, Isobe H, Homma T, Tanaka T, Negishi K, Doi K, Fujita T, Nakamura E, A water-soluble fullerene vesicle alleviates angiotensin II-induced oxidative stress in human umbilical venous endothelial cells. Hypertens Res 31(1)p141-151(2008)
Application to cultured cells (HUVECs).
5) Sango K, Yanagisawa H, Kato K, Kato N, Hirooka H, Watabe K: Differential Effects of High Glucose and Methylglyoxal on Viability and Polyol Metabolism in Immortalized Adult Mouse Schwann Cells. Open Diabetes J. 1, p1-11(2008)
Application to cultured cell from mouse.

产品信息:

Product name Code Specifications Application NOTICE
Anti HEL monoclonal antibody(clone 5F12) MHL-021P 20ug of IgG Immunohistochemistry Current product
Anti HEL monoclonal antibody(clone 5H4) MHL-020P 20ug of IgG Immunohistochemistry Discontinued

使用说明书
免疫组织化学方法
技术信息
克隆#5F12和5H4的比较

Instruction manual (PDF) Immunohistochemical procedure Technical information
Comparison of clone 5F12 and 5H4 (PDF)