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Fructanase Mixture
(Ultrapure, recombinant, powder)
20,000 Units
Prices exclude VAT
Content: | 20,000 Units |
Shipping Temperature: | Ambient |
Storage Temperature: | Below -10oC |
Formulation: | Supplied as a freeze-dried powder |
Physical Form: | Powder |
Stability: | Minimum 1 year at < -10oC. Check vial for details. |
Enzyme Activity: | Fructanase |
EC Number: | endo-Inulinase: 3.2.1.7, exo-Inulinase: 3.2.1.80 |
CAZy Family: | GH32 |
CAS Number: | 9001-57-4, 9025-67-6, 37288-56-5 |
Synonyms: | endo-inulinase: 1-beta-D-fructan fructanohydrolase exo-inulinase: fructan β-fructosidase |
Source: | Aspergillus niger |
Concentration: | endo-inulinase: Supplied at ~ 100 U/mL exo-inulinase: Supplied at ~ 2,000 U/mL |
Expression: | Recombinant from Aspergillus niger |
Specificity: | endo-Inulinase: endo-acting hydrolysis of (2,1)-β-D-fructosidic linkages in inulin. exo-Inulinase: Hydrolysis of terminal, non-reducing β-D-fructofuranoside residues in β-D-fructofuranosides. |
Unit Definition: | endo-Inulinase: One Unit of endo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from inulin (20 mg/mL) in sodium acetate buffer (100 mM), pH 4.5. exo-Inulinase: One Unit of exo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from kestose (5 mg/mL) in sodium acetate buffer (100 mM), at pH 4.5 at 40oC. |
Temperature Optima: | 60oC |
pH Optima: | 4.5 |
Fructanase, Amyloglucosidase (E-AMGDF) and Isoamylase (E-ISAMY) are used in the enzyme hydrolysis step of two validated methods for the determination of polydextrose (a low molar mass dietary fiber) in foods: AOAC method 2000.11 and Chinese GB Standard 5009.245-2016.
Components: exo-Inulinase 20,000 U (on kestose at 40oC), endo-inulinase 1000 U (on fructan at 40oC).
Note: This product has been purified to remove α-galactosidase, β-glucanase and pectinase which interfere with the use of the preparation in the measurement of fructan (AOAC Method 997.08) and in the measurement of Polydextrose (AOAC Method 2000.11).
Here is our complete list of all our CAZyme products.
Validation of Methods
Magazyme试剂盒Polygalacturonic Acid PGA P-PGACT
上海金畔生物科技有限公司代理Mezazyme酶试剂盒全线产品,欢迎访问Megazyme试剂盒官网了解更多信息
Polygalacturonic Acid (ultrapure)
10 g
Prices exclude VAT
Content: | 10 g |
Shipping Temperature: | Ambient |
Storage Temperature: | Ambient |
Physical Form: | Powder |
Stability: | > 10 years under recommended storage conditions |
CAS Number: | 9049-37-0 |
Source: | Citrus pectin |
Purity: | > 95% |
Monosaccharides (%): | Galacturonic acid: Galactose: Arabinose: Rhamnose: Xylose: Glucose = 96: 1.0: 0.2: 1.2: 1.0: 0.3 |
Main Chain Glycosidic Linkage: | α-1,4 |
Substrate For (Enzyme): | Pectate Lyase, endo-Polygalacturonase |
This product has been discontinued (read more).
High purity Polygalacturonic Acid (ultrapure) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. Note that for the assay of endo-polygalacturonanase we recommend the use of P-PGACIT as the most suitable substrate.
Please note that this product was formerly described as Polygalacturonic Acid (PGA) (P-PGACT).
Magazyme试剂盒beta-galactosidase Escherichia coli E-ECBGAL
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β-Galactosidase (Escherichia coli)
1,200 Units
Prices exclude VAT
Content: | 1,200 Units |
Shipping Temperature: | Ambient |
Storage Temperature: | 4oC |
Formulation: | In 3.2 M ammonium sulphate |
Physical Form: | Suspension |
Stability: | > 4 years at 4oC |
Enzyme Activity: | β-Galactosidase |
EC Number: | 3.2.1.23 |
CAZy Family: | GH2 |
CAS Number: | 9031-11-2 |
Synonyms: | beta-D-galactoside galactohydrolase |
Source: | Escherichia coli |
Molecular Weight: | 116,000 |
Concentration: | Supplied at ~ 900 U/mL |
Expression: | From Escherichia coli |
Specificity: | Hydrolysis of terminal non-reducing β-D-galactose residues in β-D-galactosides. |
Specific Activity: | ~ 35 U/mg (40oC, pH 6.5 on p-nitrophenyl-β-D-galactoside) |
Unit Definition: | One Unit of β-galactosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol per minute from p-nitrophenyl-β-D-galactoside (10 mM) in sodium phosphate buffer (100 mM), pH 6.5 at 40oC. |
Temperature Optima: | 40oC |
pH Optima: | 6.5 |
Application examples: | For use in research. |
This product has been discontinued (read more).
High purity β-galactosidase (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Browse our complete CAZy enzyme products range.
Magazyme试剂盒alpha-Glucuronidase Assay Kit K-AGLUA AGLUA
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α-Glucuronidase Assay Kit
50 assays (manual) / 200 assays (microplate)
Prices exclude VAT
Content: | 50 assays (manual) / 200 assays (microplate) |
Shipping Temperature: | Ambient |
Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
Stability: | > 2 years under recommended storage conditions |
Analyte: | α-Glucuronidase |
Assay Format: | Spectrophotometer, Microplate |
Detection Method: | Absorbance |
Wavelength (nm): | 340 |
Signal Response: | Increase |
Linear Range: | 17.4 to 174 mU/mL of α-D-glucuronidase per assay |
Limit of Detection: | 17 mU/mL |
Reaction Time (min): | ~ 25 min |
Application examples: | Enzyme preparations and other materials. |
Method recognition: | Novel method |
This product has been discontinued (read more).
The α-D-Glucuronidase test kit is a simple, reliable and accurate method for the measurement and analysis of α-D-glucuronidase in various enzyme preparations. The kit contains a pure aldotriouronic acid substrate and an α-D-glucuronidase (GH67) control enzyme.
New, improved substrate.
This kit contains highly purified, borohydride reduced aldotriouronic acid with a terminal α-D-glucuronic acid substitution, which is an excellent substrate for α-D-glucuronidases from GH67. This substrate can be also used for the measurement of α-D-glucuronidases from GH115, however the rate of hydrolysis compared to GH67 is reduced. Previously this kit contained a mixture of borohydride reduced aldouronic acids (tri:tetra:penta).
View more test kits for enzyme activity measurement.
- Very competitive price (cost per test)
- All reagents stable for > 2 years as supplied
- Simple format
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Standard included
- Suitable for manual, microplate and auto-analyser formats
Magazyme试剂盒CM-Pachyman P-CMPAC
上海金畔生物科技有限公司代理Mezazyme酶试剂盒全线产品,欢迎访问Megazyme试剂盒官网了解更多信息
CM-Pachyman
4 g
Prices exclude VAT
Content: | 4 g |
Shipping Temperature: | Ambient |
Storage Temperature: | Ambient |
Physical Form: | Powder |
Stability: | > 10 years under recommended storage conditions |
CAS Number: | 69552-83-6 |
Source: | Poria Coco |
Monosaccharides (%): | Glucose = 80 |
Main Chain Glycosidic Linkage: | β-1,3 |
Substrate For (Enzyme): | endo-1,3-β-Glucanase |
This product has been discontinued (read more).
High purity CM-Pachyman for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Carboxymethylated, (DS ~ 0.3) highly purified pachyman. A soluble/gelatinous substrate for the assay of endo-1,3-β-D-glucanase.
Browse our wide range of polysaccharide products.