上海金畔生物科技有限公司代理Takara Clontech酶试剂盒全线产品，欢迎访问官网了解更多产品信息和订购。

人 iPS细胞向定型内胚层细胞定向分化系统
品牌	Code No.	产品名称	包装量	价格（元）	说明书	数量
Cellartis	Y30035	Cellartis® Definitive Endoderm Differentiation Kit with DEF-CS™ Culture System	1 Kit	￥5,743

Cellartis定向分化获取内胚层细胞试剂盒 (Cellartis Definitive Endoderm Differentiation Kit)

The Cellartis Definitive Endoderm Differentiation Kit用于定向分化hiPS获取definitive endoderm (DE) cells。本产品包含所有必需的培养基和coating材料。本产品还包括新一代的Cellartis DEF-CS 100 Culture System用于起始hiPS的维持培养用。使用本产品获得的DE细胞，可以作为起始材料进行定向分化至内胚层来源的细胞类型(如肝细胞，胰腺细胞)。Cellartis也提供分化DE细胞获取肝细胞的试剂盒（Cellartis Hepatocyte Differentiation Kit (Cat. No. Y30050)；Cellartis也提供诱导分化hiPS获取肝细胞的试剂盒（Cellartis iPS Cell to Hepatocyte Differentiation System (Cat. No. Y30055)。 ■ 产品特点 · Efficient differentiation—generates >80% SOX17-positive cells · Robust protocol—successful definitive endoderm (DE) cell generation from more than 25 different hiPS cell lines · Complete kit—media and ready-to-use coating for a seven-day protocol to reproducibly differentiate hiPS cells into DE cells · Start with a homogeneous population of hiPS cells—kit includes the Cellartis DEF-CS 100 Culture System to maintain undifferentiated cells prior to the start of DE cell differentiation     ■ 产品详情请点击：

mRNA expression profiles of definitive endoderm cells being generated from two hiPS cell lines (ChiPSC18 and P11025) using the Cellartis Definitive Endoderm Differentiation Kit with DEF-CS Culture Medium. These graphs track the relative temporal mRNA expression levels of various markers during differentiation from hiPS cells (starting on Day 0) into DE cells (by Day 7), as measured by RT-qPCR.

上海金畔生物科技有限公司代理Takara Clontech酶试剂盒全线产品，欢迎访问官网了解更多产品信息和订购。

人iPS细胞培养系统
品牌	Code No.	产品名称	包装量	价格（元）	说明书	数量
Cellartis	Y30010	DEF-CS™ 500	1 Kit	￥5,979

Human iPS和Human ES增殖用培养基 (DEF-CS Culture System, all-in-one format)

作为一款高端创新型的产品，Cellartis DEF-CS培养系统使得单细胞干细胞操作成为常规的操作。该产品是专门针对人类诱导干细胞（hiPS）和人类胚胎干细胞（hES）研发的高效率增殖用培养基产品体系。该产品系统为all-in-one型，包括了所有组分，客户不需要另外求购组分。该产品是成分确定的培养基产品，而且不需要饲养层。DEF-CS既可以实现单细胞培养，也可以用于传统的iPS培养模式以及大规模干细胞增殖。在使用DEF-CS培养基增殖干细胞时，几乎没有背景分化的问题，这使得细胞筛选不再必需。作为一款创新产品，利用DEF-CS培养干细胞时，可以使用酶消化法（enzymatic passaging）实现需要单细胞操作，这一特点十分有利于高通量细胞鉴别筛选（high-throughput screening）、转染（transfection）、框架接种（scaffold seeding）等。   ■ 产品特点 · Feeder-free and chemically defined culture system for efficient expansion of human iPS cells · Ideal system for culturing iPS cells; cells maintain pluripotency and long-term potential for self-renewal and differentiation · Complete kit includes 500 ml basal medium, additives, and coating compound · Robust system provides high reproducibility and stable growth · Maintains cells in an undifferentiated state with virtually no background differentiation   ■ 产品应用 · Scale-up and mass production of human iPS cells · Single-cell culture of human iPS cells · Transfection and reprogramming · High-throughput screening · Tissue engineering (seeding cells on a scaffold)   ■ 产品详情请点击：

Robust growth of human induced pluripotent stem (iPS) cells in the Cellartis DEF-CS Culture System. The number of iPS cells was quantified after being cultured for three weeks using either the Cellartis DEF-CS Culture System, a reference feeder system, or four other stem cell culture systems.

参考文献：

1. Sivertsson, Louise, et al. "Hepatic differentiation and maturation of human embryonic stem cells cultured in a perfused three-dimensional bioreactor." Stem cells and development 22.4 (2012): 581-594. 2. Hanson, Charles, et al. "Transplantation of human embryonic stem cells onto a partially wounded human cornea in vitro." Acta ophthalmologica 91.2 (2013): 127-130. 3. Norrman, Karin, et al. "Distinct gene expression signatures in human embryonic stem cells differentiated towards definitive endoderm at single-cell level." Methods 59.1 (2013): 59-70. 4. Ulvestad, Maria, et al. "Drug metabolizing enzyme and transporter protein profiles of hepatocytes derived from human embryonic and induced pluripotent stem cells." Biochemical pharmacology 86.5 (2013): 691-702. 5. Ramirez JM, et al. Side scatter intensity is highly heterogeneous in undifferentiated pluripotent stem cells and predicts clonogenic self-renewal. Stem Cells Dev.2013 Jun 15;22(12):1851-1860. 6. Borestrom, Cecilia, et al. “Footprint-free human induced pluripotent stem cells from articular cartilage with redifferentiation capacity: A first step toward a clinical-grade cell source.” Stem Cells Trans. Med. (2014) 3, 433-447. 7. Kia, Richard, et al. "MicroRNA-122: a novel hepatocyte-enriched in vitro marker of drug-induced cellular toxicity." Toxicological Sciences (2014): kfu269. 8. Valton, Julien, et al. "Efficient strategies for TALEN-mediated genome editing in mammalian cell lines."  Methods 69.2 (2014): 151-170. 9. Zandén, Carl, et al. "Stem cell responses to plasma surface modified electrospun polyurethane scaffolds." Nanomedicine: Nanotechnology, Biology and Medicine 10.5 (2014): 949-958. 10. Asplund, Annika, et al. “One Standardized Differentiation Procedure Robustly Generates Homogenous Hepatocyte Cultures Displaying? Metabolic Diversity from a Large Panel of Human Pluripotent Stem Cells” Stem Cell Rev and Rep (2015)

上海金畔生物科技有限公司代理Takara Clontech酶试剂盒全线产品，欢迎访问官网了解更多产品信息和订购。

人iPS细胞培养系统
品牌	Code No.	产品名称	包装量	价格（元）	说明书	数量
Cellartis	Y30012	Cellartis® DEF-CS™ 500 COAT-1	4 ml	询价
Cellartis	Y30010	DEF-CS™ 500	1 Kit	￥5,979
Cellartis	Y30016	Cellartis® DEF-CS™ 500 Additives	1 set	询价

Human iPS和Human ES增殖用培养基 (DEF-CS Culture System, all-in-one format)

作为一款高端创新型的产品，Cellartis DEF-CS培养系统使得单细胞干细胞操作成为常规的操作。该产品是专门针对人类诱导干细胞（hiPS）和人类胚胎干细胞（hES）研发的高效率增殖用培养基产品体系。该产品系统为all-in-one型，包括了所有组分，客户不需要另外求购组分。该产品是成分确定的培养基产品，而且不需要饲养层。DEF-CS既可以实现单细胞培养，也可以用于传统的iPS培养模式以及大规模干细胞增殖。在使用DEF-CS培养基增殖干细胞时，几乎没有背景分化的问题，这使得细胞筛选不再必需。作为一款创新产品，利用DEF-CS培养干细胞时，可以使用酶消化法（enzymatic passaging）实现需要单细胞操作，这一特点十分有利于高通量细胞鉴别筛选（high-throughput screening）、转染（transfection）、框架接种（scaffold seeding）等。   ■ 产品特点 · Feeder-free and chemically defined culture system for efficient expansion of human iPS cells · Ideal system for culturing iPS cells; cells maintain pluripotency and long-term potential for self-renewal and differentiation · Complete kit includes 500 ml basal medium, additives, and coating compound · Robust system provides high reproducibility and stable growth · Maintains cells in an undifferentiated state with virtually no background differentiation   ■ 产品应用 · Scale-up and mass production of human iPS cells · Single-cell culture of human iPS cells · Transfection and reprogramming · High-throughput screening · Tissue engineering (seeding cells on a scaffold)   ■ 产品详情请点击：

Robust growth of human induced pluripotent stem (iPS) cells in the Cellartis DEF-CS Culture System. The number of iPS cells was quantified after being cultured for three weeks using either the Cellartis DEF-CS Culture System, a reference feeder system, or four other stem cell culture systems.

参考文献：

1. Sivertsson, Louise, et al. "Hepatic differentiation and maturation of human embryonic stem cells cultured in a perfused three-dimensional bioreactor." Stem cells and development 22.4 (2012): 581-594. 2. Hanson, Charles, et al. "Transplantation of human embryonic stem cells onto a partially wounded human cornea in vitro." Acta ophthalmologica 91.2 (2013): 127-130. 3. Norrman, Karin, et al. "Distinct gene expression signatures in human embryonic stem cells differentiated towards definitive endoderm at single-cell level." Methods 59.1 (2013): 59-70. 4. Ulvestad, Maria, et al. "Drug metabolizing enzyme and transporter protein profiles of hepatocytes derived from human embryonic and induced pluripotent stem cells." Biochemical pharmacology 86.5 (2013): 691-702. 5. Ramirez JM, et al. Side scatter intensity is highly heterogeneous in undifferentiated pluripotent stem cells and predicts clonogenic self-renewal. Stem Cells Dev.2013 Jun 15;22(12):1851-1860. 6. Borestrom, Cecilia, et al. “Footprint-free human induced pluripotent stem cells from articular cartilage with redifferentiation capacity: A first step toward a clinical-grade cell source.” Stem Cells Trans. Med. (2014) 3, 433-447. 7. Kia, Richard, et al. "MicroRNA-122: a novel hepatocyte-enriched in vitro marker of drug-induced cellular toxicity." Toxicological Sciences (2014): kfu269. 8. Valton, Julien, et al. "Efficient strategies for TALEN-mediated genome editing in mammalian cell lines."  Methods 69.2 (2014): 151-170. 9. Zandén, Carl, et al. "Stem cell responses to plasma surface modified electrospun polyurethane scaffolds." Nanomedicine: Nanotechnology, Biology and Medicine 10.5 (2014): 949-958. 10. Asplund, Annika, et al. “One Standardized Differentiation Procedure Robustly Generates Homogenous Hepatocyte Cultures Displaying? Metabolic Diversity from a Large Panel of Human Pluripotent Stem Cells” Stem Cell Rev and Rep (2015)

上海金畔生物科技有限公司代理Takara Clontech酶试剂盒全线产品，欢迎访问官网了解更多产品信息和订购。

人iPS细胞来源肝脏细胞
品牌	Code No.	产品名称	包装量	价格（元）	说明书	数量
Cellartis	Y10133	Cellartis® Enhanced hiPS-HEP v2 (from ChiPSC12) Kit	1 kit	￥21,173
Cellartis	Y10134	Cellartis® Enhanced hiPS-HEP v2 (from ChiPSC18) Kit	1 kit	￥21,173
Cellartis	Y10135	Cellartis® Enhanced hiPS-HEP v2 (from ChiPSC22) Kit	1 kit	￥21,173

人iPS干细胞分化来的肝细胞

Cellartis enhanced hiPS-HEP v2 kits 提供了可持续供应的、成熟的人诱导多能干细胞（hiPS）来源的肝脏细胞。在超过14天的检测窗口期，hiPS-HEP细胞拥有功能性的药物代谢机制，使其成为体外药物研发、药物代谢、和相关毒性研究的理想选择；具备成熟、稳健的代谢特征，支持其作为代谢疾病模型。方便使用的试剂盒包括完全的培养基和组份，用于长期检测。与其他肝脏细胞模型相比，培养的Cellartis hiPS-HEP细胞存活时间更长，进而拥有更长的检测窗口用于慢性-毒性实验和作为代谢疾病模型研究。   ■ 产品特点 · Complete kit for thawing, plating, and culturing the provided cells · Highly homogeneous population with consistent performance between batches and over time · Cells are functional and stable for a 14-day assay window · Cells show long-term, stable CYP450 activities · Cells express key hepatocyte proteins, including α1AT, Alb, CK18, HNF4α, and E-cadherin · Cells secrete physiologically relevant levels of albumin and urea · Cells demonstrate functional insulin and glucose regulation · Cells show phase I and II enzyme activities · Cells demonstrate LDL uptake   ■ 产品组成 · 1 vial of ～1.2 × 107 cells · Hepatocyte thawing medium · Hepatocyte coating · Hepatocyte plating medium · Hepatocyte washing medium · Hepatocyte long-term maintenance medium   ■ 产品应用 · Toxicity testing, including chronic toxicity assays · Drug discovery and development · Metabolic studies · Metabolic disease modeling

■ 产品详情请点击：

Cellartis 人iPS细胞来源的肝脏细胞可以作为长期的、可靠的肝脏疾病模型。

Cellartis human induced pluripotent stem (iPS) cell-derived hepatocytes are a long-lasting, reliable liver disease model. Ideal for long-term studies, these mature, functional, pure hepatocytes allow you to generate consistent results with low batch-to-batch variability. Cellartis iPSC-derived hepatocytes can be used for extended culture significantly longer than human primary hepatocytes—allowing you to get more data from your chronic toxicity studies.

Cellartis enhanced hiPS-HEP cells可以稳定表达CYP450 活性超过21天。

CYP450 activity is stable in enhanced hiPS-HEP cells over 21 days. LC/MS was used to analyze CYP450 activity in cultured enhanced hiPS-HEP cells previously derived from the hiPS cell lines ChiPSC12, ChiPSC18, and ChiPSC22 (abbreviated as C12, C18, and C22). CYP3A (Panel A), CYP2C9 (Panel B), CYP1A (Panel C), and CYP2C19 (Panel D) activities in enhanced hiPS-HEP cells are stable over an extended culture time. Cryopreserved human primary hepatocytes (hphep), which are functional in culture for a significantly shorter time than enhanced hiPS-HEP cells, were thawed and cultured for 20 hours, and their data are shown as the black bar in each panel.

Cellartis enhanced hiPS-HEP细胞在20天的培养中表达白蛋白（Albumin）。

Albumin is present in enhanced hiPS-HEP cells through 20 days in culture. Panel A. Representative images of enhanced hiPS-HEP cells from human iPS cell line ChiPSC18 (C18) taken 12 days after thawing (right), compared to cryopreserved human primary hepatocytes (hphep) taken 24 hr after thawing (left). Cells were stained for albumin and DAPI. Panel B. Albumin secretion as measured by ELISA; n=2 for enhanced hiPS-HEP cells, with the exception of C18 at 20 days (n=1), and n=3 donors for hphep.

（以上图片均来源于Takara Bio USA, Inc.）

参考文献：

1. Heins et al. Stem Cells 2004; 22: 367-376.United States National Stem Cell Bank; http://www.nationalstemcellbank.org. 2. Mantel N et al. Potential markers of attenuation of YF virus after infection of stem cell-derived human hepatocytes with wild-type Asibi or live-attenuated YF17D virus.Supplement to The American Journal of tropical Medicine and Hygiene, Volume 83, November 2010, Number 5, abstract 12. 3. Yildirimman R et al. Human embryonic stem cell derived hepatocyte-like cells as a tool for in vitro hazard assessment of chemical carcinogenicity. Toxicol. Sci. 2011 Dec; 124(2): 278-90. 4. Ulvestad M et al. Drug metabolizing enzyme and transporter protein profi les of hepatocytes derived from human embryonic and induced pluripotent stem cells. Biochem Pharmacol. 2013 Sep 1; 86(5):691-702.