细胞活性(荧光)测试盒 CellQuanti-Blue Cell Viability Assay Kits (货号:CQBL-048/CQBL-05K)
包装:48T/5000T
- 英文名:CellQuanti-Blue Cell Viability Assay Kits
- 级别:高效药物筛选
- 品牌:BioAssay Systems
Club Cell Protein (CC16) Human ELISA, 人Clara 细胞蛋白Elisa Kit|Biovendor|上海金畔生物科技有限公司
Club Cell Protein (CC16) Human ELISA, 人Clara 细胞蛋白Elisa Kit
包装:96wells
Cell Signaling Technology品牌代理商
上海金畔生物科技有限公司是Cell Signaling Technology品牌代理商 ,欢迎访问官网了解更多产品信息和订购。
Cell Signaling Technology
Cell Signaling Technology, Inc (CST) 是美国著名的生物公司和细胞信号转导研究的,提供特色的信号转导检测用抗体及磷酸化抗体、ELISA试剂盒、激酶等产品。
Cell Signaling Technology, Inc (CST) 是美国著名的生物公司和细胞信号转导研究的,提供特色的信号转导检测用抗体及磷酸化抗体、ELISA试剂盒、激酶等产品。CST总部位于美国波士顿,毗邻哈佛、麻省理工等著名学府的雄厚人才资源,拥有专业的生产和研发队伍,发表的文章多次收录在Nature, Cell, Journal of Immunology 等杂志上。其高质量的产品和专业的研发精神已被全球客户推崇为“细胞信号转导研究的金标准”!
Cell Signaling Technology (CST) is a private, family-owned company, founded by scientists and dedicated to providing the world’s highest quality, innovative research and diagnostic products to accelerate biological understanding and enable personalized medicine. Our employees operate worldwide from our U.S. headquarters in Massachusetts, and our offices in the Netherlands, China, and Japan.
产品列表:
No. |
品牌 |
货号 |
名称 |
规格 |
1 |
cst |
9601 |
Phospho-(Ser) 14-3-3 Binding Motif Antibody |
100 µl |
2 |
cst |
9606S |
Phospho-(Ser) 14-3-3 Binding Motif (4E2) Mouse mAb |
100 µl |
3 |
cst |
5522S |
14-3-3γ(D15B7) Rabbit mAb |
100 µl |
4 |
cst |
9769T |
14-3-3 Family Antibody Sampler Kit |
1kit |
5 |
cst |
9640S |
14-3-3ηAntibody |
100ul |
6 |
cst |
2855T |
Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb |
20ul |
7 |
cst |
6592S |
Annexin V-FITC Early Apoptosis Detection Kit |
1kit |
8 |
cst |
5492S |
BrdU Cell Proliferation Chemiluminescent Assay Kit |
1kit |
9 |
cst |
6813S |
BrdU Cell Proliferation Assay Kit |
1kit |
10 |
cst |
5723S |
Caspase-3 Activity Assay Kit |
1kit |
11 |
cst |
8020S |
Cyclic GMP XP® Chemiluminescent Assay Kit |
1kit |
12 |
cst |
5939S |
AIF (D39D2) XP® Rabbit mAb (Sepharose® Bead Conjugate) |
400ul |
13 |
cst |
8050S |
Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb (Magnetic Bead Conjugate) |
400ul |
14 |
cst |
9646S |
Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb (Sepharose® Bead Conjugate) |
400ul |
15 |
cst |
9563S |
Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (Magnetic Bead Conjugate) |
400ul |
Cell Biolabs特约代理
Cell Biolabs特约代理货品多且全,目前,以我们美国公司为主体,与欧美100多个品牌建立了正式的代理与合作,基本涵盖所有的生物试剂门类,特别是二三线高质量品牌产品,优势更大!
Cell Biolabs是美国著名细胞学试剂品牌,作为前沿的细胞学分析检测试剂盒生产商,为生物技术和研究类客户提供基于细胞功能研究以及疾病等方面的产品和技术服务。Cell Biolabs的诸多产品均采用了技术,比如病毒纯化方面,其开发的病毒吸附Matrix能有效的识别不同的病毒包膜蛋白而特异吸附不同的病毒颗粒。另外,其Cell Biolabs拥有的Platinum (Plat-E, Plat-A and Plat-GP) 逆转录病毒包装细胞系,在生长稳定性和病毒包装滴度方面表现优异,成为众多逆转录病毒包装细胞系中的细胞。
Cell Biolabs, Inc. proudly develops and commercializes innovative technologies and tools for life science research. We are committed to providing the best products that facilitate discovery of the mechanisms underlying cell function and disease.
Our unique, cutting-edge products are currently used around the world in the research laboratories of universities, government institutes, biotechnology and pharmaceutical companies.
Cell Biolabs特约代理产品列表:
cell biolabs |
AAV-200 |
ViraDuctin™ AAV Transduction Kit (10 Transductions) |
10 transductions |
cell biolabs |
AAV-201 |
ViraDuctin™ AAV Transduction Kit (50 Transductions) |
50 transductions |
cell biolabs |
AAV-300 |
AAV2 Null Control Virus |
50 μL |
cell biolabs |
AAV-301 |
AAV1-GFP Control Virus |
50 μL |
cell biolabs |
AAV-302 |
AAV2-GFP Control Virus |
50 μL |
cell biolabs |
AAV-303 |
AAV3-GFP Control Virus |
50 μL |
cell biolabs |
AAV-305 |
AAV5-GFP Control Virus |
50 μL |
cell biolabs |
AAV-306 |
AAV6-GFP Control Virus |
50 μL |
cell biolabs |
AAV-310 |
AAV2-Cre Control Virus |
50 μL |
cell biolabs |
AAV-320 |
AAV2-Luc Control Virus |
50 μL |
cell biolabs |
AAV-400 |
pAAV-GFP Control Vector |
10 µg |
cell biolabs |
AAV-401 |
pAAV-Cre Control Vector |
10 µg |
cell biolabs |
AAV-402 |
pAAV-LacZ Control Vector |
10 µg |
cell biolabs |
AAV-410 |
pscAAV-GFP Control Vector |
10 µg |
cell biolabs |
AD-200 |
ViraDuctin™ Adenovirus Transduction Kit (10 Transductions) |
10 transductions |
cell biolabs |
AD-201 |
ViraDuctin™ Adenovirus Transduction Kit (50 Transductions) |
50 transductions |
cell biolabs |
ADV-001 |
Null Control Recombinant Adenovirus |
50 μL |
cell biolabs |
ADV-002 |
β-Galalactosidase Recombinant Adenovirus |
50 μL |
cell biolabs |
ADV-003 |
SEAP (Secretory Alkaline Phosphatase) Recombinant Adenovirus |
50 μL |
cell biolabs |
ADV-004 |
GFP Recombinant Adenovirus |
50 μL |
cell biolabs |
ADV-005 |
Cre Recombinant Adenovirus |
50 μL |
cell biolabs |
ADV-008 |
Firefly Luciferase Recombinant Adenovirus |
50 μL |
cell biolabs |
ADV-100 |
Hypoxia Inducible Factor-1 alpha Recombinant Adenovirus |
50 μL |
cell biolabs |
ADV-101 |
VEGF Recombinant Adenovirus |
50 μL |
cell biolabs |
ADV-103 |
Interferon γ Recombinant Adenovirus |
50 μL |
cell biolabs |
ADV-104 |
p38α Recombinant Adenovirus |
50 μL |
cell biolabs |
ADV-105 |
p38α (Dominant Negative) Recombinant Adenovirus |
50 μL |
cell biolabs |
ADV-112 |
ERK2 Recombinant Adenovirus |
50 μL |
cell biolabs |
ADV-113 |
ERK2 (Dominant Negative) Recombinant Adenovirus |
50 μL |
cell biolabs |
ADV-114 |
JNK1 Recombinant Adenovirus |
50 μL |
cell biolabs |
ADV-115 |
JNK1 (Dominant Negative) Recombinant Adenovirus |
50 μL |
cell biolabs |
ADV-118 |
MEK1 (Dominant Negative) Recombinant Adenovirus |
50 μL |
cell biolabs |
ADV-119 |
MEK1 (Constitutively Active) Recombinant Adenovirus |
50 μL |
cell biolabs |
ADV-120 |
MKK3 Recombinant Adenovirus |
50 μL |
cell biolabs |
ADV-121 |
MKK3 (Dominant Negative) Recombinant Adenovirus |
50 μL |
cell biolabs |
ADV-122 |
MKK3 (Constitutively Active) Recombinant Adenovirus |
50 μL |
cell biolabs |
ADV-123 |
MKK6 Recombinant Adenovirus |
50 μL |
cell biolabs |
ADV-124 |
MKK6 (Dominant Negative) Recombinant Adenovirus |
50 μL |
cell biolabs |
ADV-125 |
MKK6 (Constitutively Active) Recombinant Adenovirus |
50 μL |
cell biolabs |
ADV-126 |
MKK7 Recombinant Adenovirus |
50 μL |
cell biolabs |
ADV-127 |
MKK7 (Dominant Negative) Recombinant Adenovirus |
50 μL |
cell biolabs |
ADV-128 |
MKK7 (Constitutively Active) Recombinant Adenovirus |
50 μL |
cell biolabs |
ADV-129 |
MEK5 Recombinant Adenovirus |
50 μL |
cell biolabs |
ADV-130 |
MEK5 (Dominant Negative) Recombinant Adenovirus |
50 μL |
cell biolabs |
ADV-131 |
MEK5 (Constitutively Active) Recombinant Adenovirus |
50 μL |
cell biolabs |
ADV-132 |
Raf 1 Recombinant Adenovirus |
50 μL |
cell biolabs |
ADV-133 |
Raf1 (Dominant Negative) Recombinant Adenovirus |
50 μL |
cell biolabs |
ADV-134 |
Raf1 (constitutively active) Recombinant Adenovirus |
50 μL |
cell biolabs |
ADV-135 |
MEKK1 Recombinant Adenovirus |
50 μL |
cell biolabs |
ADV-136 |
MEKK1 (Dominant Negative) Recombinant Adenovirus |
50 μL |
cell biolabs |
ADV-137 |
MAPKAPK2 Recombinant Adenovirus |
50 μL |
cell biolabs |
ADV-138 |
MAPKAPK2 (Dominant Negative) Recombinant Adenovirus |
50 μL |
cell biolabs |
ADV-139 |
MAPKAPK2 (Constitutively Active) Recombinant Adenovirus |
50 μL |
cell biolabs |
ADV-145 |
Ras N17 (Dominant Negative) Recombinant Adenovirus |
50 μL |
Premix WST-1 Cell Proliferation Assay System酶试剂盒Takara Clontech
上海金畔生物科技有限公司代理Takara Clontech酶试剂盒全线产品,欢迎访问官网了解更多产品信息和订购。
Premix WST-1 Cell Proliferation Assay System | ||||||
品牌 | Code No. | 产品名称 | 包装量 | 价格(元) | 说明书 | 数量 |
Takara | MK400 | Premix WST-1 Cell Proliferation Assay System | 2,500 次 | ¥3,405 | ![]() |
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Premix WST-1 Cell Proliferation Assay System酶试剂盒Takara Clontech
上海金畔生物科技有限公司代理Takara Clontech酶试剂盒全线产品,欢迎访问官网了解更多产品信息和订购。
Premix WST-1 Cell Proliferation Assay System | ||||||
品牌 | Code No. | 产品名称 | 包装量 | 价格(元) | 说明书 | 数量 |
Takara | MK400 | Premix WST-1 Cell Proliferation Assay System | 2500 Tests | ¥3,405 | ![]() |
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Luna® Cell Ready 一步法 RT-qPCR 试剂盒 货 号 #E3030S-NEB酶试剂 New England Biolabs
上海金畔生物科技有限公司代理New England Biolabs(NEB)酶试剂全线产品,欢迎访问官网了解更多产品信息和订购。
Luna® Cell Ready 一步法 RT-qPCR 试剂盒 收藏
Download:
- isoschizomers |
- compatible ends |
- single letter code
特性
无需纯化,直接从细胞到 RNA 定量
• 试剂盒包含 100 次裂解反应和 500 次染料一步法 RT-qPCR 反应
• 有效裂解多种细胞系的 10 至 10 万个细胞
• 仅需 15 分钟即可完成细胞裂解、RNA 释放和基因组 DNA 去除
• 联合使用 LunaWarmStart® RT 和 Hot Start Taq,提高了热稳定性,且可在室温下建立反应体系
• 可单独购买裂解模块:Luna Cell Ready 裂解模块(NEB#E3032)
概述
Luna Cell Ready 染料一步法 RT-qPCR 试剂盒为直接染料法 RNA 检测和定量提供了所有必要的组分,且无需进行 RNA 提取和纯化。Luna Cell Ready 染料一步法 RT-qPCR 试剂盒包括:1)Luna Cell Ready 裂解模块(NEB#E3032S)和 2)Luna 通用一步法 RT-qPCR Kit(NEB#E3005L)。
细胞培养常用于替代活体生物来分析基因表达或对治疗的反应。传统上使用柱提法或化学试剂法从处理过的细胞中提取和纯化 RNA。
Luna Cell Ready 裂解模块整合了 DNase I 和 Luna Cell Ready 蛋白酶的功能,仅需 15 分钟即可完成细胞裂解、RNA 释放和基因组 DNA 去除。裂解模块包含独特的 Luna Cell Ready RNA 保护试剂,可在细胞裂解过程中保持 RNA 完整性。50 µl 裂解体系下可对 10-10 万个细胞进行裂解反应。2 µl 裂解产物 (相当于 0.2–4000 个细胞的 RNA) 即可实现 20 µl 体系的 RT-qPCR 反应。与其它 Luna 产品一样,裂解缓冲液包含惰性蓝色示踪染料,使整个加样流程可视化。
Luna 通用一步法 RT-qPCR 试剂盒可兼容大多数实时荧光定量仪器的 SYBR/FAM 荧光通道,进行目标 RNA 的实时定量分析。在该试剂盒中,联合使用热启动 Taq DNA 聚合酶与新型 Luna WarmStart 反转录酶,通过适配体可逆抑制来双重控制酶活性。Luna 通用一步法 RT-qPCR 试剂盒可以直接将 Luna Cell Ready 裂解模块制备的细胞裂解物中的 RNA 转录本进行检测和定量。
下表列出的细胞系可使用 Luna Cell Ready 裂解模块裂解。这些细胞进行 5 log 梯度稀释(100000-10 细胞/50 µl 裂解反应),并取 1 ul 裂解产物进行 20 µl 体系的一步法 RT-qPCR 反应。每个细胞系的线性结果区域显示在最后一列。此外,经测试一些昆虫细胞系也能用上述裂解液裂解。
细胞系 |
|
特性 |
菌种 |
50 ml 裂解体系中的细胞数量 |
A549 |
Adherent |
H. sapiens, Lung, carcinoma |
10 – 100,000 |
HEK293 |
Adherent |
H. sapiens, Kidney |
10 – 100,000 |
HeLa |
Adherent |
H. sapiens, Cervix, adenocarcinoma |
10 – 100,000 |
HepG2 |
Adherent |
H. sapiens, Liver, carcinoma |
10 – 100,000 |
NCI-H460 |
Adherent |
H. sapiens, Lung, carcinoma |
10 – 100,000 |
SK-N-SH |
Adherent |
H. sapiens, Brain, Neuroblastoma |
10 – 100,000 |
U2Os |
Adherent |
H. sapiens, Bone, osteosarcoma |
10 – 10,000 |
Jurkat |
Suspension |
H. sapiens, T lymphocyte, leukemia |
10 – 100,000 |
K-562 |
Suspension |
H. sapiens, Lymphoblast, leukemia |
10 – 1,000 |
图一:Luna Cell Ready 一步法 RT-qPCR 实验流程
Luna Cell Ready 一步法 RT-qPCR 试剂盒提供了直接从细胞(50 µl 体系可裂解高达 10 万个细胞)到 RNA 检测和定量的所有组分。Luna Cell Ready 裂解模块整合了 DNase I 和 Luna Cell Ready 蛋白酶的功能,仅需 15 分钟即可完成细胞裂解、RNA 释放和基因组 DNA 去除。2 µl 裂解产物(相当于 0.2–4000 个细胞中的 RNA)即可实现 20 µl 体系的 RT-qPCR 反应。
图二:Luna Cell Ready 染料一步法 RT-qPCR 试剂盒可直接对 5-log 梯度稀释细胞的裂解物进行灵敏精准的 RNA 定量
梯度稀释的 A549 细胞(100,000-10)使用标准反应条件(37°C 裂解 10 分钟,25°C 灭活 5 分钟),在 50 µl 的 Luna Cell Ready 裂解体系(NEB#E3032)中裂解。使用 Luna 通用一步法 RT-qPCR 试剂盒(NEB#E3005)加入 1 μl 细胞裂解物(相当于 20 μl RT-qPCR 反应体系中 0.2-2,000 个细胞)对目的基因(GOI)进行定量,每个浓度的样品都做了重复。结果展示了两个高频靶标(A):β-actin 和一个低频靶标(B):SMG1。右图展示了扩增效率(E)和线性关系(R2)。
图三:Luna Cell Ready 染料一步法 RT-qPCR 试剂盒与提纯 RNA 均可对 5-logs 细胞梯度样品进行精准可信的 RNA 定量
梯度稀释的 A549 细胞(100,000-10)使用标准反应条件(37°C 裂解 10 分钟,25°C 灭活 5 分钟),在 50 µl 的 Luna Cell Ready 裂解体系(NEB#E3032)中裂解,也可使用柱提法提纯 RNA。A.使用 Luna 通用一步法 RT-qPCR 试剂盒(NEB#E3005)对目的基因(GOI)进行定量,加入 1 μl 细胞裂解物(实心圆)或纯化的 RNA(空心圆)(相当于 20 µl RT-qPCR 反应中有 0.2-2,000 个细胞),每个浓度的样品都做了重复。左图:在 5-logs 细胞梯度中检测高频靶标 β-actin和两个低频靶标 ARF3 和 Tubulin。每个靶标的扩增效率在图中左下方显示。B.在 A 图中 5-log 细胞梯度稀释样品的目标基因相对于 β-actin 的 Cq 值(ΔCq(GOI)),平均 Cqs 通过横线指示。
图四:Luna Cell Ready 染料一步法 RT-qPCR 试剂盒的扩增效率及灵敏度均优于其它市售的细胞裂解一步法 RT-qPCR 试剂盒。
梯度稀释的 A549 细胞(100,000-10)使用标准反应条件,在 50 µl 的 Luna Cell Ready 裂解体系(NEB#E3032)中裂解。相同的样品使用 Bio-Rad(SingleShot SYBR Green One-Step Kit, #172-5095)、Qiagen(SingleShot SYBR Green One-Step Kit, #172-5095)和 Thermo Fisher(Cells-to-CT 1-Step PowerSYBR Green Kit, A25600)并分别按照厂家说明书进行裂解。然后使用每个试剂盒中的一步法 RT-qPCR 模块,加入 1 μl 细胞裂解物(相当于 20 μl RT-qPCR 反应体系中 0.2-2,000 个细胞),对目的基因进行定量,每个浓度的样品都做了重复。对于所有试剂盒,5-log 梯度稀释细胞均显示了 β-actin,(高频靶标)和 RPL32、Tubulin (低频靶标)的扩增。为了使结果标准化,将总荧光的 12% 设置为阈值。图中显示了扩增效率(E)及最高与最低的 Cq 值。
图五:对 24 个靶标测试显示,Luna Cell Ready 染料一步法 RT-qPCR 试剂盒与其它市售的细胞裂解一步法 RT-qPCR 试剂盒相比,灵敏度最高。
2500 个 A549 细胞,使用标准反应条件,在 50 µl 的 Luna Cell Ready 裂解体系(NEB#E3032)中裂解。或使用市售的 Bio-Rad(SingleShot SYBR Green One-Step Kit, #172-5095)、Qiagen(SingleShot SYBR Green One-Step Kit, #172-5095)和 Thermo Fisher(Cells-to-CT 1-Step PowerSYBR Green Kit, A25600)并分别按照厂家说明书进行裂解。每个试剂盒做两个生物重复。然后使用来自每个试剂盒的一步法 RT-qPCR 模块,加入 1 μl 细胞裂解物(相当于 20 µl RT-qPCR 反应中 50 个细胞),对 24 个目标基因进行定量,每个生物样品均做重复。结果显示了 NEB (实心橙色圆圈),BioRad(空心正方形),Qiagen(空心三角形)和 Thermo Fisher(十字形)的平均 Cqs。为了使结果标准化,将总荧光的 12% 设置为阈值。Luna Cell Ready 染料一步法 RT-qPCR 试剂盒显示了不同表达水平下 23/24 的基因 Cq 值最早,平均比 Bio-Rad 快2.3 Cq,比 Qiagen 快 3.8 Cq,比 Thermo Fisher 快 3.6 Cq。
随该产品提供的试剂
储存(°C) |
|
浓度 |
|
Luna Cell Ready Lysis Buffer |
-20 |
2 X |
Luna Cell Ready RNA Protection Reagent |
-20 |
25 X |
Luna Cell Ready Protease |
-20 |
25 X |
Luna Cell Ready Stop Solution |
-20 |
10 X |
DNase I (RNase-free) |
-20 |
10 X |
Luna® WarmStart® RT Enzyme Mix |
-20 |
20 X |
Luna® Universal One-Step Reaction Mix |
-20 |
2 X |
Nuclease-free Water |
-20 |
实验需要但不提供的试剂耗材
• 磷酸盐缓冲液(PBS)
• 目标特异性引物
• 细胞
• Eppendorf 管,PCR 联管
• PCR 板
• qPCR 仪器
• 移液器和枪头(为减少交叉污染,应使用带滤芯的枪头)
储存温度
-20℃
Luna® Cell Ready 探针一步法 RT-qPCR 试剂盒 货 号 #E3031S-NEB酶试剂 New England Biolabs
上海金畔生物科技有限公司代理New England Biolabs(NEB)酶试剂全线产品,欢迎访问官网了解更多产品信息和订购。
Luna® Cell Ready 探针一步法 RT-qPCR 试剂盒 收藏
Download:
- isoschizomers |
- compatible ends |
- single letter code
特性
无需纯化,直接从细胞到 RNA 定量
• 试剂盒包含 100 次裂解反应和 500 次探针一步法 RT-qPCR 反应
• 有效裂解多种细胞系的 10 至 10 万个细胞
• 仅需 15 分钟即可完成细胞裂解、RNA 释放和基因组 DNA 去除
• 配合使用 LunaWarmStart® RT 和 Hot Start Taq,提高了热稳定性且可在室温下建立反应体系
• 可单独购买裂解模块:Luna Cell Ready 裂解模块(NEB#E3032)
概述
Luna Cell Ready 探针一步法 RT-qPCR 试剂盒为直接探针法 RNA 检测和定量提供了所有必要的组分,且无需进行 RNA 提取和纯化。Luna Cell Ready 探针一步法 RT-qPCR 试剂盒包含两个模块:1)Luna Cell Ready 裂解模块(NEB#E3032S)和 2)Luna 通用探针一步法 RT-qPCR Kit(NEB#E3006L)。
细胞培养常用于替代活体生物来分析基因表达或对治疗的反应。传统上使用柱提法或化学试剂法从处理过的细胞中提取和纯化 RNA。
Luna Cell Ready 裂解模块整合了 DNase I 和 Luna Cell Ready 蛋白酶的功能,仅需 15 分钟即可完成细胞裂解、RNA 释放和基因组 DNA 去除。裂解模块包含独特的 Luna Cell Ready RNA 保护试剂,可在细胞裂解过程中保持 RNA 完整性。50 µl 裂解体系下可对 10-10 万个细胞进行裂解反应。2 µl 裂解产物 (相当于 0.2–4000 个细胞的 RNA) 即可实现 20 µl 体系的 RT-qPCR 反应。与其它 Luna 产品一样,裂解缓冲液包含惰性蓝色示踪染料,使整个加样流程可视化。
Luna 通用探针一步法 RT-qPCR 试剂盒可兼容大多数实时荧光定量仪器的各个通道,进行目标 RNA 的实时定量分析。在该试剂盒中,联合使用热启动 Taq DNA 聚合酶与新型 Luna WarmStart 反转录酶,通过适配体可逆抑制来双重控制酶活性。Luna 通用探针一步法 RT-qPCR 试剂盒可以直接将 Luna Cell Ready 裂解模块制备的细胞裂解物中的 RNA 转录本进行检测和定量(单重或多重)。
下表列出的细胞系可使用 Luna Cell Ready 裂解模块裂解。这些细胞进行 5 log 梯度稀释(100000-10 细胞/50 µl 裂解反应),并取 1 ul 裂解产物进行 20 µl 体系的一步法 RT-qPCR 反应。每个细胞系的线性结果区域显示在最后一列。此外,经测试一些昆虫细胞系也能用上述裂解液裂解。
表一:经验证过的细胞系
细胞系 |
|
特性 |
菌种 |
50 ml 裂解体系中的细胞数量 |
A549 |
Adherent |
H. sapiens, Lung, carcinoma |
10 – 100,000 |
HEK293 |
Adherent |
H. sapiens, Kidney |
10 – 100,000 |
HeLa |
Adherent |
H. sapiens, Cervix, adenocarcinoma |
10 – 100,000 |
HepG2 |
Adherent |
H. sapiens, Liver, carcinoma |
10 – 100,000 |
NCI-H460 |
Adherent |
H. sapiens, Lung, carcinoma |
10 – 100,000 |
SK-N-SH |
Adherent |
H. sapiens, Brain, Neuroblastoma |
10 – 100,000 |
U2Os |
Adherent |
H. sapiens, Bone, osteosarcoma |
10 – 10,000 |
Jurkat |
Suspension |
H. sapiens, T lymphocyte, leukemia |
10 – 100,000 |
K-562 |
Suspension |
H. sapiens, Lymphoblast, leukemia |
10 – 1,000 |
Luna Cell Ready 一步法 RT-qPCR 试剂盒提供了直接从细胞(50 µl 体系可裂解高达 10 万个细胞)到 RNA 检测和定量的所有组分。Luna Cell Ready 裂解模块整合了 DNase I 和 Luna Cell Ready 蛋白酶的功能,仅需 15 分钟即可完成细胞裂解、RNA 释放和基因组 DNA 去除。2 µl 裂解产物(相当于 0.2–4000 个细胞中的 RNA)即可实现 20 µl 体系的 RT-qPCR 反应。
图二:Luna Cell Ready 探针一步法 RT-qPCR 试剂盒可直接对 5-log 梯度稀释细胞的裂解物进行灵敏精准的 RNA 定量
梯度稀释的 A549 细胞(100,000-10)使用标准反应条件(37°C 裂解 10 分钟,25°C 灭活 5 分钟),在 50 µl 的 Luna Cell Ready 裂解体系(NEB#E3032)中裂解。使用 Luna 通用探针一步法 RT-qPCR 试剂盒(NEB#E3006),加入 1 μl 细胞裂解物(相当于 20 μl RT-qPCR 反应体系中 0.2-2,000 个细胞)对目的基因进行定量,每个浓度的样品都做了重复。结果展示了两个高频靶标:β-actin(Texas Red)和 GAPDH(FAM),一个低频靶标:RPL32(HEX),的多重结果(A)和单重结果(B),以及相应的扩增效率(E)和线性关系(R2)。(C)所有三个目标的多重和单重的 Cq 叠加证明了这些结果的一致性。
三:Luna Cell Ready 探针一步法 RT-qPCR 试剂盒与提纯 RNA 均可对 5-logs 细胞梯度样品进行精准可信的 RNA 定量
梯度稀释的 A549 细胞(100,000-10)使用标准反应条件(37°C 裂解 10 分钟,25°C 灭活 5 分钟),在 50 µl 的 Luna Cell Ready 裂解体系(NEB#E3032)中裂解,也可使用柱提法提纯 RNA。A.使用 Luna 通用探针一步法 RT-qPCR 试剂盒(NEB#E3006)对目的基因进行定量,加入 1 μl 细胞裂解物(实心圆,左)或纯化的 RNA(空心圆,右)(上样量相当于 20 µl RT-qPCR 反应中有 0.2-2,000 个细胞),每个浓度的样品都做了重复。在 5-logs 细胞梯度中检测高频靶标 β-actin 和两个低频靶标 ARF3 和 Tubulin。每个靶标的扩增效率在图中左下方显示。
图四:Luna Cell Ready 染料一步法 RT-qPCR 试剂盒的扩增效率及灵敏度均优于其它市售的细胞裂解一步法 RT-qPCR 试剂盒。
梯度稀释的 A549 细胞(100,000-10)使用标准反应条件,在 50 µl 的 Luna Cell Ready 裂解体系(NEB#E3032)中裂解。相同的样品使用 Bio-Rad(SingleShot™ Probes One-Step Kit for Cell Lysis and RT-qPCR, #172-5070)、Qiagen(FastLane Cell Probe Kit, #216413)和 Thermo Fisher(Cells-to-CT 1-Step TaqMan Kit, A25603)并分别按照厂家说明书进行裂解。然后使用每个试剂盒中的一步法 RT-qPCR 模块,加入 1 μl 细胞裂解物(相当于 20 μl RT-qPCR 反应体系中 0.2-2,000 个细胞),对目的基因进行定量,每个浓度的样品都做了重复。5-log 梯度稀释的细胞通过多重实验测试了 β-actin (Tye)、GAPDH (FAM)和 RPL32 (Cy5)的扩增。为了使结果标准化,将每个试剂盒总荧光值的 10%(Tye)、5%(FAM)、15%(Cy5)设置为阈值。图中显示了扩增效率(E)及最高与最低的 Cq 值。
随该产品提供的试剂
储存(°C) |
浓度 |
|
Luna Cell Ready Lysis Buffer |
-20 |
2 X |
Luna Cell Ready RNA Protection Reagent |
-20 |
25 X |
Luna Cell Ready Protease |
-20 |
25 X |
Luna Cell Ready Stop Solution |
-20 |
10 X |
DNase I (RNase-free) |
-20 |
10 X |
Luna® WarmStart® RT Enzyme Mix |
-20 |
20 X |
Luna® Universal Probe One-Step Reaction Mix |
-20 |
2 X |
Nuclease-free Water |
-20 |
• 磷酸盐缓冲液(PBS)
• 目标特异性引物
• 细胞
• Eppendorf 管
• PCR联管
• PCR 板
• qPCR 仪器
• 移液器和枪头(为减少交叉污染,应使用带滤芯的枪头)
-20℃
Luna® Cell Ready 裂解模块 货 号 #E3032S-NEB酶试剂 New England Biolabs
上海金畔生物科技有限公司代理New England Biolabs(NEB)酶试剂全线产品,欢迎访问官网了解更多产品信息和订购。
Luna® Cell Ready 裂解模块 收藏
Download:
- isoschizomers |
- compatible ends |
- single letter code
特性
概述
细胞培养常用于替代活体生物来分析基因表达或对治疗的反应。传统上使用柱提法或化学试剂法从处理过的细胞中提取和纯化 RNA.Luna Cell Ready 裂解模块无需进行传统的 RNA 提取步骤即可评估RNA表达水平,是一种快速、方便且灵敏的替代方案。该模块可同时进行细胞裂解、RNA 释放、基因组 DNA 去除,获得的细胞裂解物可直接使用Luna通用一步法 RT-qPCR 试剂盒进行 RT-qPCR 分析。与其它 Luna 产品一样,裂解缓冲液包含惰性蓝色示踪染料,使整个加样流程可视化。
细胞系 |
特性 |
菌种 |
50 ml 裂解体系中的细胞数量 |
A549 |
Adherent |
H. sapiens, Lung, carcinoma |
10 – 100,000 |
HEK293 |
Adherent |
H. sapiens, Kidney |
10 – 100,000 |
HeLa |
Adherent |
H. sapiens, Cervix, adenocarcinoma |
10 – 100,000 |
HepG2 |
Adherent |
H. sapiens, Liver, carcinoma |
10 – 100,000 |
NCI-H460 |
Adherent |
H. sapiens, Lung, carcinoma |
10 – 100,000 |
SK-N-SH |
Adherent |
H. sapiens, Brain, Neuroblastoma |
10 – 100,000 |
U2Os |
Adherent |
H. sapiens, Bone, osteosarcoma |
10 – 10,000 |
Jurkat |
Suspension |
H. sapiens, T lymphocyte, leukemia |
10 – 100,000 |
K-562 |
Suspension |
H. sapiens, Lymphoblast, leukemia |
10 – 1,000 |
图一:Luna Cell Ready 一步法 RT-qPCR 实验流程
Luna Cell Ready 裂解模块整合了 DNase I 和 Luna Cell Ready 蛋白酶的功能,可简单高效的进行细胞裂解,仅需 15 分钟即可完成细胞裂解、RNA 释放和基因组 DNA 去除。2 µl 裂解产物(相当于 0.2–4000 个细胞中的 RNA)即可实现 20 µl 体系的 RT-qPCR 反应。
试剂盒组分
随该产品提供的试剂
储存(°C) |
浓度 |
|
Luna Cell Ready Lysis Buffer |
-20 |
2 X |
Luna Cell Ready RNA Protection Reagent |
-20 |
25 X |
Luna Cell Ready Protease |
-20 |
25 X |
Luna Cell Ready Stop Solution |
-20 |
10 X |
DNase I (RNase-free) |
-20 |
10 X |
- 磷酸盐缓冲液(PBS)
- 目标特异性引物
- 细胞
- Eppendorf 管
- PCR 联管
- PCR 板
- 移液器和枪头(为减少交叉污染,应使用带滤芯的枪头)
储存温度
-20℃
Engrafted Cell Markers酶试剂盒Takara Clontech
上海金畔生物科技有限公司代理Takara Clontech酶试剂盒全线产品,欢迎访问官网了解更多产品信息和订购。
Engrafted Cell Markers | ||||||
品牌 | Code No. | 产品名称 | 包装量 | 价格(元) | 说明书 | 数量 |
Cellartis | Y40400 | STEM101™ | 50 μg | ¥1,958 | ![]() |
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Cellartis | Y40410 | STEM121™ | 50 μg | ¥1,958 | ![]() |
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Cellartis | Y40420 | STEM123™ | 50 μg | ¥1,958 | ![]() |
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监控人类细胞移植用途的抗体 (Antibodies for Detecting Human Cells Transplanted into Mice and Rats) |
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STEM101 detects nuclei of transplanted human neural stem cells in the olfactory bulb of a mouse brain. | ||||||||||||
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STEM121 detects migration and differentiation of transplanted human neural stem cells in the hippocampus of a mouse brain. | ||||||||||||
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STEM123 detects human GFAP+ astrocytes differentiated from human neural stem cells after transplantation into a mouse brain. | ||||||||||||
参考文献: | ||||||||||||
1. Cummings BJ, et al. (2005) Human neural stem cells differentiate and promote locomotor recovery in spinal cord-injured mice. PNAS 102: 14069-14074. 2. Guzman R, et al. (2007) Long-term monitoring of transplanted human neural stem cells in developmental and pathological contexts with MRI. PNAS 104: 10211-10216. 3. Tamaki SJ, et al. (2009) Neuroprotection of Host Cells by Human Central Nervous System Stem Cells in a Mouse Model of Infantile Neuronal Ceroid Lipofuscinosis. Cell Stem Cell 5:310-319. 4. Salazar DL, et al. (2010) Human Neural Stem Cells Differentiate and Promote Locomotor Recovery in an Early Chronic Spinal Cord Injury NOD-scid Mouse Model. PLoS ONE 5: e12272. 5. Kelly S, et al. Transplanted human fetal neural stem cells survive, migrate, and differentiate in ischemic rat cerebral cortex. PNAS (2004) 101:11839 11844. 6. Cummings BJ, et al. Human neural stem cells differentiate and promote locomotor recovery in spinal cord-injured mice. PNAS (2005) 102: 14069-14074. 7. Tamaki SJ, et al. Neuroprotection of Host Cells by Human Central Nervous System Stem Cells in a Mouse Model of Infantile Neuronal Ceroid Lipofuscinosis. Cell Stem Cell (2009) 5:310-319. 8. Kallur T, et al. Human Fetal Cortical and Striatal Neural Stem Cells Generate Region-Specific Neurons In Vitro and Differentiate Extensively to Neurons After Intrastriatal Transplantation in Neonatal Rats. J Neurosci Res. (2006) 84:1630-1644. 9. Salazar DL, et al. Human Neural Stem Cells Differentiate and Promote Locomotor Recovery in an Early Chronic Spinal Cord Injury NOD-scid Mouse Model. PLoS ONE (2010) 5: e12272. 10. Tamaki SJ, et al. (2009) Neuroprotection of Host Cells by Human Central Nervous System Stem Cells in a Mouse Model of Infantile Neuronal Ceroid Lipofuscinosis. Cell Stem Cell 5:310-319. 11. Salazar DL, et al. (2010) Human Neural Stem Cells Differentiate and Promote Locomotor Recovery in an Early Chronic Spinal Cord Injury NOD-scid Mouse Model. PLoS ONE 5:e12272. 12. Cummings BJ, et al. (2005) Human neural stem cells differentiate and promote locomotor recovery in spinal cord-injured mice. PNAS 102: 14069-14074. |
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Human Stem Cell Culture System (DEF-CS, all-in-one format)酶试剂盒Takara Clontech
上海金畔生物科技有限公司代理Takara Clontech酶试剂盒全线产品,欢迎访问官网了解更多产品信息和订购。
Human Stem Cell Culture System (DEF-CS, all-in-one format) | ||||||
品牌 | Code No. | 产品名称 | 包装量 | 价格(元) | 说明书 | 数量 |
Cellartis | Y30010 | Cellartis® DEF-CS™ 500 Culture System | 1 Kit | ¥5,979 | ![]() |
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Human iPS和Human ES增殖用培养基 (DEF-CS Culture System, all-in-one format) |
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Robust growth of human induced pluripotent stem (iPS) cells in the Cellartis DEF-CS Culture System. The number of iPS cells was quantified after being cultured for three weeks using either the Cellartis DEF-CS Culture System, a reference feeder system, or four other stem cell culture systems. | ||||||||||
参考文献: | ||||||||||
1. Sivertsson, Louise, et al. "Hepatic differentiation and maturation of human embryonic stem cells cultured in a perfused three-dimensional bioreactor." Stem cells and development 22.4 (2012): 581-594. 2. Hanson, Charles, et al. "Transplantation of human embryonic stem cells onto a partially wounded human cornea in vitro." Acta ophthalmologica 91.2 (2013): 127-130. 3. Norrman, Karin, et al. "Distinct gene expression signatures in human embryonic stem cells differentiated towards definitive endoderm at single-cell level." Methods 59.1 (2013): 59-70. 4. Ulvestad, Maria, et al. "Drug metabolizing enzyme and transporter protein profiles of hepatocytes derived from human embryonic and induced pluripotent stem cells." Biochemical pharmacology 86.5 (2013): 691-702. 5. Ramirez JM, et al. Side scatter intensity is highly heterogeneous in undifferentiated pluripotent stem cells and predicts clonogenic self-renewal. Stem Cells Dev.2013 Jun 15;22(12):1851-1860. 6. Borestrom, Cecilia, et al. “Footprint-free human induced pluripotent stem cells from articular cartilage with redifferentiation capacity: A first step toward a clinical-grade cell source.” Stem Cells Trans. Med. (2014) 3, 433-447. 7. Kia, Richard, et al. "MicroRNA-122: a novel hepatocyte-enriched in vitro marker of drug-induced cellular toxicity." Toxicological Sciences (2014): kfu269. 8. Valton, Julien, et al. "Efficient strategies for TALEN-mediated genome editing in mammalian cell lines." Methods 69.2 (2014): 151-170. 9. Zandén, Carl, et al. "Stem cell responses to plasma surface modified electrospun polyurethane scaffolds." Nanomedicine: Nanotechnology, Biology and Medicine 10.5 (2014): 949-958. 10. Asplund, Annika, et al. “One Standardized Differentiation Procedure Robustly Generates Homogenous Hepatocyte Cultures Displaying? Metabolic Diversity from a Large Panel of Human Pluripotent Stem Cells” Stem Cell Rev and Rep (2015) |
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iPS Cell to Hepatocyte Differentiation System酶试剂盒Takara Clontech
上海金畔生物科技有限公司代理Takara Clontech酶试剂盒全线产品,欢迎访问官网了解更多产品信息和订购。
iPS Cell to Hepatocyte Differentiation System | ||||||
品牌 | Code No. | 产品名称 | 包装量 | 价格(元) | 说明书 | 数量 |
Cellartis | Y30055 | Cellartis® iPS Cell to Hepatocyte Differentiation System | 1 Kit | ¥13,846 | ![]() |
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Cellartis | Y30051 | Cellartis® Hepatocyte Maintenance Medium | 100 ml | ¥2,198 | ![]() |
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iPS向肝脏细胞定向分化操作系统 (Cellartis iPS Cell to Hepatocyte Differentiation System) |
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Immunocytochemistry analysis of hepatocyte differentiation. hiPS cells were differentiated into functional hepatocytes using the Cellartis iPS Cell to Hepatocyte Differentiation System. Hepatocytes were immunostained to detect early hepatic markers HNF4α (red, nuclear) and CK18 (green) on days 14 and 21. As the hepatocytes matured, expression of liver-specific markers CYP3A (red, cytoplasmic) and Albumin (green) increased as seen on day 28, as expected. Cell nuclei were stained with DAPI (blue). | |||||||||||||||||
参考文献: | |||||||||||||||||
Asplund, Annika, et al.One Standardized Differentiation Procedure Robustly Generates Homogenous Hepatocyte Cultures Displaying? Metabolic Diversity from a Large Panel of Human Pluripotent Stem Cells. Stem Cell Rev and Rep (2015). | |||||||||||||||||
用户分享: | |||||||||||||||||
◆ 东海大学医学部-纸谷聪英教授 利用人iPS细胞体外构建多囊性肝病(Polycystic liver disease)模型 |
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纸谷聪英教授使用Cellartis iPS Cell to Hepatocyte Differentiation System(Y30055)构建肝脏疾病模型的研究成果已刊登在Stem Cell Research。 【论文】 Akihide, K., Hiromi, C., Kinuyo, I., Emi, A., Kota, T., & Tatehiro, K., et al. (2018). An in vitro model of polycystic liver disease using genome-edited human inducible pluripotent stem cells. Stem Cell Research, 32, 17-24. |
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实验数据 | |||||||||||||||||
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图:使用 Cellartis iPS Cell to Hepatocyte Differentiation System(Y30055)获得的人iPSC来源肝前体细胞(iPS-HPCs)诱导分化为胆管状Cyst结构。 |
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(引用自文献Akihide Kamiya et al., Stem Cell Research, 2018) | |||||||||||||||||
访谈时间: | |||||||||||||||||
1. 开始使用Cellartis iPS Cell to Hepatocyte Differentiation System(Y30055)的契机是什么? | |||||||||||||||||
很多研究组已经报道了多种从人iPS细胞诱导分化形成肝脏细胞的方法,我们研究组也在做相关工作。由于细胞分化不稳定,我担心使用饲养层细胞培养人iPS细胞会对肝脏分化产生影响,因此决定尝试使用Cellartis iPS细胞无饲养层培养系统Cellartis DEF-CS™ 500 Culture System(Y30010)和肝脏分化系统Cellartis iPS Cell to Hepatocyte Differentiation System(Y30055)。 | |||||||||||||||||
2.实际使用后感觉如何? | |||||||||||||||||
与之前使用的饲养层培养方法相比,Y30010能更简便高效的进行培养;使用Y30055进行的肝脏分化非常稳定,进一步提升了实验效率。 | |||||||||||||||||
3. 对暂未使用该制品的用户有什么寄语? | |||||||||||||||||
人iPS细胞向肝脏细胞分化过程中,细胞密度对分化效率有很大影响,进而影响实验结果。我认为Cellartis系统的优点主要在于各个实验阶段偏差小,结果稳定,适合追求实验效率的人。 | |||||||||||||||||
◆ 东京医科齿科大学-柿沼晴教授 先天性肝纤维化模型的开发和病理阐明的研究 |
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柿沼晴教授使用Cellartis iPS Cell to Hepatocyte Differentiation System(Y30055)构建肝脏疾病模型的研究成果已刊登在Journal of Hepatology。 | |||||||||||||||||
【论文】 | |||||||||||||||||
Tsunoda, T., Kakinuma, S., Miyoshi, M., Kamiya, A., Kaneko, S., & Sato, A., et al. (2019). Loss of fibrocystin promotes interleukin-8-dependent proliferation and ctgf production of biliary epithelium. Journal of Hepatology. | |||||||||||||||||
访谈时间: | |||||||||||||||||
1.开始使用Cellartis iPS Cell to Hepatocyte Differentiation System(Y30055)的契机是什么? | |||||||||||||||||
一起进行研究的老师使用后能够进行稳定的培养,我们研究室也正好想利用无饲养层培养系统,所以开始使用了Cellartis DEF-CS™ 500 Culture System(Y30010)和肝脏分化系统Cellartis iPS Cell to Hepatocyte Differentiation System(Y30055)。 | |||||||||||||||||
2.实际使用后感觉如何? | |||||||||||||||||
Y30010无饲养层培养系统使用比较简便,同时能够稳定的培养人iPS细胞。这次实验中,利用Y30055分化至肝前体细胞(iPS-HPCs),进一步诱导分化成了胆管细胞,我认为诱导分化的再现性好。不仅分化至成熟肝细胞谱系,同时相比于以往报道的方法,再现性更稳定。 | |||||||||||||||||
3. 对暂未使用该制品的用户有什么寄语? | |||||||||||||||||
在人iPS细胞的培养和分化至肝细胞谱系的诱导中,即使是对人iPS细胞使用经验尚浅的实验者,也能够进行培养并得到稳定的再现。 | |||||||||||||||||
Human Stem Cell Derived Hepatocytes酶试剂盒Takara Clontech
上海金畔生物科技有限公司代理Takara Clontech酶试剂盒全线产品,欢迎访问官网了解更多产品信息和订购。
Human Stem Cell Derived Hepatocytes | ||||||
品牌 | Code No. | 产品名称 | 包装量 | 价格(元) | 说明书 | 数量 |
Cellartis | Y10133 | Cellartis® Enhanced hiPS-HEP v2 (from ChiPSC12) Kit | 1 kit | ¥21,173 | ![]() |
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Cellartis | Y10134 | Cellartis® Enhanced hiPS-HEP v2 (from ChiPSC18) Kit | 1 kit | ¥21,173 | ![]() |
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Cellartis | Y10135 | Cellartis® Enhanced hiPS-HEP v2 (from ChiPSC22) Kit | 1 kit | ¥21,173 | ![]() |
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人iPS干细胞分化来的肝细胞 |
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■ 产品详情请点击:![]() |
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Cellartis 人iPS细胞来源的肝脏细胞可以作为长期的、可靠的肝脏疾病模型。 | ||||||||||
Cellartis human induced pluripotent stem (iPS) cell-derived hepatocytes are a long-lasting, reliable liver disease model. Ideal for long-term studies, these mature, functional, pure hepatocytes allow you to generate consistent results with low batch-to-batch variability. Cellartis iPSC-derived hepatocytes can be used for extended culture significantly longer than human primary hepatocytes—allowing you to get more data from your chronic toxicity studies. | ||||||||||
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Cellartis enhanced hiPS-HEP cells可以稳定表达CYP450 活性超过21天。 | ||||||||||
CYP450 activity is stable in enhanced hiPS-HEP cells over 21 days. LC/MS was used to analyze CYP450 activity in cultured enhanced hiPS-HEP cells previously derived from the hiPS cell lines ChiPSC12, ChiPSC18, and ChiPSC22 (abbreviated as C12, C18, and C22). CYP3A (Panel A), CYP2C9 (Panel B), CYP1A (Panel C), and CYP2C19 (Panel D) activities in enhanced hiPS-HEP cells are stable over an extended culture time. Cryopreserved human primary hepatocytes (hphep), which are functional in culture for a significantly shorter time than enhanced hiPS-HEP cells, were thawed and cultured for 20 hours, and their data are shown as the black bar in each panel. | ||||||||||
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Cellartis enhanced hiPS-HEP细胞在20天的培养中表达白蛋白(Albumin)。 | ||||||||||
Albumin is present in enhanced hiPS-HEP cells through 20 days in culture. Panel A. Representative images of enhanced hiPS-HEP cells from human iPS cell line ChiPSC18 (C18) taken 12 days after thawing (right), compared to cryopreserved human primary hepatocytes (hphep) taken 24 hr after thawing (left). Cells were stained for albumin and DAPI. Panel B. Albumin secretion as measured by ELISA; n=2 for enhanced hiPS-HEP cells, with the exception of C18 at 20 days (n=1), and n=3 donors for hphep. | ||||||||||
(以上图片均来源于Takara Bio USA, Inc.) | ||||||||||
参考文献: | ||||||||||
1. Heins et al. Stem Cells 2004; 22: 367-376.United States National Stem Cell Bank; http://www.nationalstemcellbank.org. 2. Mantel N et al. Potential markers of attenuation of YF virus after infection of stem cell-derived human hepatocytes with wild-type Asibi or live-attenuated YF17D virus.Supplement to The American Journal of tropical Medicine and Hygiene, Volume 83, November 2010, Number 5, abstract 12. 3. Yildirimman R et al. Human embryonic stem cell derived hepatocyte-like cells as a tool for in vitro hazard assessment of chemical carcinogenicity. Toxicol. Sci. 2011 Dec; 124(2): 278-90. 4. Ulvestad M et al. Drug metabolizing enzyme and transporter protein profi les of hepatocytes derived from human embryonic and induced pluripotent stem cells. Biochem Pharmacol. 2013 Sep 1; 86(5):691-702. |
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Human iPS Cell Generation™ All-in-One Vector酶试剂盒Takara Clontech
上海金畔生物科技有限公司代理Takara Clontech酶试剂盒全线产品,欢迎访问官网了解更多产品信息和订购。
Human iPS Cell Generation™ All-in-One Vector | ||||||
品牌 | Code No. | 产品名称 | 包装量 | 价格(元) | 说明书 | 数量 |
Takara | 3671 | Human iPS Cell Generation™ All-in-One Vector | 10 μg | ¥10,014 | ![]() |
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■ 制品说明 | |||||||||||||
本制品是制备Human iPS细胞诱导用逆转录病毒所使用的载体。在高效、高表达逆转录病毒载体pDON-5 DNA(Code No.: 3658)上,插入了iPS细胞诱导用基因OCT3/4和SOX2、KLF4、LIN28和NANOG,各基因以Thosea asigna virus 的2A*1序列连接在一个载体上。本载体与Retrovirus Packaging Kit Ampho(Code No.: 6161)中的pGP Vector以及pE-ampho Vector共转染至逆转录病毒制备用细胞G3T-hi(Code No.: 6163)后,可以制备iPS诱导用逆转录病毒。如此制备的重组逆转录病毒由于和RetroNectin®(Recombinant Human Fibronectin Fragment)(Code No.: T100A/B)有高亲和性,所以在向目的细胞中导入基因时,使用经RetroNectin®处理后的培养皿或平板,可以得到较高的基因导入率,有效提高iPS细胞的诱导效率。 *1 5种基因转录在同一个mRNA上,翻译时在2A序列的特定位点被分割,使5种蛋白质可以等量表达。 |
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■ 保存 | |||||||||||||
-20℃。 | |||||||||||||
■ 各载体的遗传信息 | |||||||||||||
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*2 更新的NM_004235.4中,CDS的5’端追加了对应9个氨基酸的碱基。本制品的KLF4基因中不含N端的这9个氨基酸的序列,已经确认可以有效诱导iPS细胞。 | |||||||||||||
■ 载体图谱 | |||||||||||||
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Human Stem Cell Derived Intestinal Epithelial Cells酶试剂盒Takara Clontech
上海金畔生物科技有限公司代理Takara Clontech酶试剂盒全线产品,欢迎访问官网了解更多产品信息和订购。
Human Stem Cell Derived Intestinal Epithelial Cells | ||||||
品牌 | Code No. | 产品名称 | 包装量 | 价格(元) | 说明书 | 数量 |
Cellartis | Y50035 | Cellartis® Intestinal Epithelial Cells (from ChiPSC18) Kit | 1 Kit | ¥20,754 | ![]() |
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来源于人类iPS细胞的小肠上皮细胞 (Intestinal Epithelial Cells Derived from Human Induced Pluripotent Stem Cells) |
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■ 产品组成 | |||||||
· 1 vial of~4.8 x 106 cells · 1 Cellartis IEC Maturation Kit(IEC Maturation Basal Medium;IEC Supplement A;IEC Supplement B;IEC Supplement C;IEC Supplement D) |
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实验例 | |||||||
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图1. 紧密连接蛋白ZO-1的免疫荧光染色结果显示,解冻后第5天,hiPSC-derived intestinal epithelial cells (IEC)细胞间形成紧密连接(如闭锁小带zonula occludens)。 | |||||||
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图2. 在解冻后第7天,与Caco-2细胞相比,hiPSC-derived intestinal epithelial cells (IEC)中高表达肠上皮标志物villin和CDX2。 | |||||||
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图3. 在解冻后第7天,与Caco-2细胞相比,hiPSC-derived intestinal epithelial cells (IEC)中高表达药物代谢酶CYP3A4和药物转运蛋白PEPT1。 | |||||||
■ 产品详情请点击:![]() |
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Human Stem Cell Derived Beta Cells酶试剂盒Takara Clontech
上海金畔生物科技有限公司代理Takara Clontech酶试剂盒全线产品,欢迎访问官网了解更多产品信息和订购。
Human Stem Cell Derived Beta Cells | ||||||
品牌 | Code No. | 产品名称 | 包装量 | 价格(元) | 说明书 | 数量 |
Cellartis | Y10100 | Cellartis® hiPS Beta Cells (from ChiPSC12) Kit | 1 Kit | ¥18,655 | ![]() |
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Cellartis | Y10106 | Cellartis® hiPS Beta Cells (from ChiPSC22) Kit | 1 Kit | ¥22,051 | ![]() |
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来源于人类iPS细胞的胰腺β细胞 (Pancreatic Beta Cells Derived from Human Induced Pluripotent Stem Cells) |
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■ 产品组成 | ||||||||||||||||
· 1 vial (4.8 x 106 viable cells) of Cellartis hiPS Beta Cells (from ChiPSC12) or ChiPSC22 · 11 vials (360 μl/vial) of Cellartis Beta Cell Supplement · 1 tube (4 ml) of Cellartis Beta Cell Coating · 1 bottle (160 ml) of Cellartis Beta Cell Basal Medium · 1 bottle (30 ml) of Cellartis Beta Cell Basal Medium 2 |
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[Cellartis hiPS Beta Cells (from ChiPSC12) 的胰岛素和 C-肽免疫荧光染色] | ||||||||||||||||
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解冻后两周的β细胞进行成熟标志胰岛素(A图) 和 C-肽 (B图)免疫荧光染色。合并数据显示了胰岛素和 C-肽共表达细胞 (C图)。细胞核使用DAPI 染色(D图)。 | ||||||||||||||||
(图片来源于Takara Bio USA, Inc.) | ||||||||||||||||
■ 技术资料 | ||||||||||||||||
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Cellartis human iPS cell-derived beta cells for diabetes drug discovery and disease modeling | ||||||||||||||||
Studying diabetes and metabolic disorders using hiPSC-derived beta cells. | ||||||||||||||||
■ 参考文献 | ||||||||||||||||
Marron, M. et al. Functional evidence for the mediation of diabetogenic T cell responses by HLA-A2.1 MHC class I molecules through transgenic expression in NOD mice. Proc Nat Acad Sci U. S. A. 99, 13753–8 (2002). | ||||||||||||||||
■ 产品详情请点击:![]() |
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Human Stem Cell Derived Definitive Endoderm Cells酶试剂盒Takara Clontech
上海金畔生物科技有限公司代理Takara Clontech酶试剂盒全线产品,欢迎访问官网了解更多产品信息和订购。
Human Stem Cell Derived Definitive Endoderm Cells | ||||||
品牌 | Code No. | 产品名称 | 包装量 | 价格(元) | 说明书 | 数量 |
Cellartis | Y10040 | Cellartis® Definitive Endoderm Cells (from ChiPSC18) | 1 Vial | ¥8,387 | ![]() |
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Cellartis定型内胚层干细胞 [Cellartis Definitive Endoderm Cells (from ChiPSC18)] |
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Immunocytochemistry of Cellartis Definitive Endoderm Cells (from ChiPSC18). Cells were thawed, cultured for two days, fixed, and stained for the pluripotency marker OCT4, the definitive endoderm marker SOX17, and the nuclear marker DAPI. Results show that more than 90% of the cell population are definitive endoderm cells and have lost pluripotency. | ||||||||||||||||||||||||||||
Human Stem Cell Culture Medium (DEF-CS Xeno-Free)酶试剂盒Takara Clontech
上海金畔生物科技有限公司代理Takara Clontech酶试剂盒全线产品,欢迎访问官网了解更多产品信息和订购。
Human Stem Cell Culture Medium (DEF-CS Xeno-Free) | ||||||
品牌 | Code No. | 产品名称 | 包装量 | 价格(元) | 说明书 | 数量 |
Cellartis | Y30045 | Cellartis® DEF-CS™ 500 Xeno-Free Culture Medium w/o antibiotics | 1 Kit | ¥2,923 | ![]() |
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Cellartis | Y30047 | Cellartis® DEF-CS™ 500 Xeno-Free 3D Spheroid Culture Medium | 1 Kit | ¥3,747 | ![]() |
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Cellartis | Y30071 | Cellartis® DEF-CS™ 500 Xeno-Free GMP Grade Basal Medium (Prototype) | 500 ml | ¥6,088 | ![]() |
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临床前扩增人干细胞培养基系列(Xeno-free) (The Xeno-Free Culture Media Product Family for Preclinical Expansion of Human iPS Cells) |
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High-quality hiPS cell aggregates grown with Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium in stirred tank bioreactors (in perfusion mode). hiPS cell aggregates grown under 4% O2 perfusion conditions were sequentially passaged three times by mechanical disruption (protocol adapted from Otsuji et al. 2014); cells were counted at passage on days 4 and 7, and at the final time point on day 11, to determine the expansion factor relative to the initial seeding number. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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High-quality hiPS cell aggregates were grown with Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium in stirred tank bioreactors (in perfusion mode). After expansion of hiPS cells as aggregates, cells were harvested on day 4 and expression of OCT4, SSEA-4, TRA-1-60 (pluripotency markers) and SSEA-1 and SOX17 (differentiation markers) was analyzed by immunostaining. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Mouse ES and iPS cell culture medium (2i)酶试剂盒Takara Clontech
上海金畔生物科技有限公司代理Takara Clontech酶试剂盒全线产品,欢迎访问官网了解更多产品信息和订购。
Mouse ES and iPS cell culture medium (2i) | ||||||
品牌 | Code No. | 产品名称 | 包装量 | 价格(元) | 说明书 | 数量 |
Cellartis | Y40031 | Cellartis® 2i mES/iPSC Culture Medium | 200 ml | ¥3,158 | ![]() |
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小鼠ES/iPS细胞培养基(2i Medium) | |||||||||||||||||
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Mouse ES and iPS cell culture medium (3i)酶试剂盒Takara Clontech
上海金畔生物科技有限公司代理Takara Clontech酶试剂盒全线产品,欢迎访问官网了解更多产品信息和订购。
Mouse ES and iPS cell culture medium (3i) | ||||||
品牌 | Code No. | 产品名称 | 包装量 | 价格(元) | 说明书 | 数量 |
Cellartis | Y40011 | Cellartis® 3i mES/iPSC Culture Medium | 200 ml | ¥4,226 | ![]() |
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小鼠ES/iPS细胞培养基(3i Medium) |
Cellartis 3i mES/iPSC Culture Medium是一款无血清的、成分确定的细胞培养基,可用于小鼠ES细胞的建系、多能性维持和增殖培养。该培养基含有三种小分子抑制剂(基于3i技术:ERK/MEK inhibitor、GSK3β inhibitor、FGFR-TK inhibitor),用于抑制分化诱导信号通路和促进细胞增殖,以及可以在无需额外添加细胞因子刺激的情况下维持多能性基态(Pluripotent ground state)。因此,Cellartis 3i mES/iPSC Culture Medium可以应用于多能性分子机制研究和分析。 Cellartis 3i mES/iPSC Culture Medium也应用于从标准条件下难建立ES细胞的小鼠品系中建立ES细胞系(Ying et al. 2008)。相较于LIF(Leukemia Inhibitory Factor)+血清或BMP(Bone Morphogenetic Protein)条件下培养的小鼠ES细胞,使用Cellartis 3i mES/iPSC Culture Medium培养的小鼠ES细胞可以持续地以双倍速度扩增 (Ying et al. 2008; Kiyonari et al. 2010; Iijima et al. 2010)。此外,同样与LIF+血清或BMP条件下培养的小鼠ES细胞相比,使用Cellartis 3i mES/iPSC Culture Medium培养的小鼠ES细胞表现出更高的多能性克隆形成效率(Clonogenicity)。 |
■ 产品特点 |
· 成分确定、无血清、无饲养层、无细胞因子 · 通过添加小分子抑制剂来抑制分化诱导通路并维持小鼠ES细胞处于多能性状态 · 支持小鼠ES细胞增殖并维持≥99%纯度 |
■ 产品应用 |
· 维持小鼠ES细胞处于多能性状态 · 从难建系的小鼠品系中制备小鼠ES细胞 · 高效制备具有完全多能性的小鼠ES细胞 |
■ 产品组成 |
· 1 bottle (200 ml) of Cellartis mES/iPSC Culture Basal Medium · 1 tube (200 μl) of Cellartis 3i mES/iPSC Supplement |
■ 产品详情请点击:![]() |
Cellartis 3i mES/iPSC Culture Medium维持小鼠ES细胞处于同质化、未分化状态。 |
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小鼠ES细胞系ES-E14TG2a分别使用Cellartis 3i mES/iPSC Culture Medium(A)和 GMEM-based medium(B)培养,通过碱性磷酸酶(Alkaline phosphatase, AKP)染色检测ES细胞多能性,左边为染色前,右边为AKP染色后结果,可以看出Cellartis 3i Medium培养的小鼠ES细胞为均一的、未分化细胞。 |
Cellartis 3i mES/iPSC Culture Medium培养建立的小鼠ES细胞系具备高嵌合率。 |
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将ES细胞注射并植入胚胎后,通过出生小鼠个体的毛色来判断ES细胞的嵌合率。从Cellartis 3i medium培养建立的ES细胞所获得的小鼠均为黑色毛,即在单代内就获得了完全是ES细胞来源的嵌合体小鼠(A),具备高嵌合率。而从常规培养基培养建立的ES细胞所获得的嵌合体小鼠毛色混杂,未完全嵌合(B)。 |
■ 参考文献 |
1. Ying, Q. L. et al. The ground state of embryonic stem cell self-renewal. Nature 453, 519–23 (2008). 2. Kiyonari, H. et al. Three inhibitors of FGF receptor, ERK, and GSK3 establishes germline-competent embryonic stem cells of C57BL/6N mouse strain with high efficiency and stability. Genesis 48, 317–327 (2010). 3. Iijima, S. et al. Effect of different culture conditions on establishment of embryonic stem cells from BALB/cAJ and NZB/BINJ mice. Cell Reprogram. 12, 679–688 (2010). |
Adherent Neural Stem Cell Culture Media酶试剂盒Takara Clontech
上海金畔生物科技有限公司代理Takara Clontech酶试剂盒全线产品,欢迎访问官网了解更多产品信息和订购。
Adherent Neural Stem Cell Culture Media | ||||||
品牌 | Code No. | 产品名称 | 包装量 | 价格(元) | 说明书 | 数量 |
Cellartis | Y40000 | RHB-Basal® | 500 ml | ¥2,259 | ![]() |
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Cellartis | Y40001 | RHB-A® | 500 ml | ¥3,627 | ![]() |
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Neural Stem Cell Culture Media | ||||||||||
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Adherent mouse neural stem cells cultured in RHB-A medium supplemented with epidermal growth factor and fibroblast growth factor-2 express neural stem cell markers, including Nestin, Vimentin (3CB2), radial glial cell marker-2 (RC2), glial fibrillary acidic protein (GFAP), and microtubule associate protein (MAP). | ||||||||||
参考文献: | ||||||||||
1. Ying QL, et al. (2003) Conversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture. Nature Biotechnology 21:183-186. 2. Conti L, et al. (2005) Niche-Independent symmetrical self-renewal of a mammalian tissue stem cell. PLoS Biology 3(9):e283. 3. Pollard SM, et al. (2006) Adherent Neural Stem (NS) cells from fetal and adult forebrain. Cerebral Cortex 16:112-120. 4. Diogo MM, et al. (2008) Optimization and integration of expansion and neural commitment of mouse embryonic stem cells. Biotechnology and Applied Biochemistry 49:105-112. 5. Pollard SM, et al. (2008) Fibroblast growth factor induces a neural stem cell phenotype in foetal forebrain progenitors and during embryonic stem cell differentiation. Molecular and Cellular Neuroscience 38:393:403. 6. Sun Y, et al. (2008) Long-term tripotent differentiation capacity of human neural stem (NS)cells in adherent culture. Molecular and Cellular Neuroscience 38:245-258. 7. Pollard SM, et al. (2009) Glioma stem cell lines expanded in adherent culture have tumor-specific phenotypes and are suitable for chemical and genetic screens. Cell Stem Cell 4:568-580. 8. Abranches E, et al. (2009) Neural differentiation of embryonic stem cells in vitro: A road map to neurogenesis in the embryo. PLoS ONE 4(7): e6286. 9. Fernandes et al. (2010) Hypoxia enhances proliferation of mouse embryonic stem cell-derived neural stem cells. Biotechnology and Bioengineering 106: 260–270. 10. Fernandes, T.G., et al. (2010) Different stages of pluripotency determine distinct patterns of proliferation, metabolism, and lineage commitment of embryonic stem cells under hypoxia. Stem Cell Research 5(1):76-89. 11. Fernandes et al. (2010) Three-dimensional cell culture microarray for high-throughput studies of stem cell fate. Biotechnology and Bioengineering 106: 106-118. 12. Hansen, et al. (2010) Neurogenic radial glia in the outer subventricular zone of human neocortex. Nature doi: 10.1038/nature08845. 13. Hook L, et al. (2011) Non-immortalized human neural stem (NS) cells as a scalable platform for cellular assays. Neurochem Int. 59(3): 432-44. |
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Mouse Feeder Cell Quantification Kit酶试剂盒Takara Clontech
上海金畔生物科技有限公司代理Takara Clontech酶试剂盒全线产品,欢迎访问官网了解更多产品信息和订购。
Mouse Feeder Cell Quantification Kit | ||||||
品牌 | Code No. | 产品名称 | 包装量 | 价格(元) | 说明书 | 数量 |
Takara | RR290 | Mouse Feeder Cell Quantification Kit | 100 Rxns | Inquire | ![]() |
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■ 制品内容(25 μl × 100次量) | ||||||||||||||||||||||||||||||
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TB Green Premix Ex Taq II(Tli RNaseH Plus) |
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4℃可保存6个月。 避光保存。避免污染。 ※长期保存时,请于-20℃保存。产品融解后请于4℃保存,并在6个月内用完。 |
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Neural Progenitor Cell Antibodies酶试剂盒Takara Clontech
上海金畔生物科技有限公司代理Takara Clontech酶试剂盒全线产品,欢迎访问官网了解更多产品信息和订购。
Neural Progenitor Cell Antibodies | ||||||
品牌 | Code No. | 产品名称 | 包装量 | 价格(元) | 说明书 | 数量 |
Takara | M194 | Anti-Mouse L7/Pcp2, Polyclonal | 0.2 mg | ¥3,244 | ![]() |
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Takara | M195 | Anti-Mouse Otp, Polyclonal | 0.2 mg | ¥3,244 | ![]() |
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Takara | M196 | Anti-Mouse Emx1, Polyclonal | 0.2 mg | ¥3,244 | ![]() |
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Takara | M198 | Anti-Mouse Otx2, Polyclonal | 0.2 mg | ¥3,244 | ![]() |
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Takara | M202 | Anti-Human L7/Pcp2, Polyclonal | 0.2 mg | ¥3,244 | ![]() |
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Takara | M227 | Anti-Human/Mouse Bf1, Polyclonal | 0.2 mg | ¥3,244 | ![]() |
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Takara | M228 | Anti-Mouse Rx, Polyclonal | 0.2 mg | ¥3,244 | ![]() |
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Takara | M229 | Anti-Human/Mouse Rx, Polyclonal (Guinea Pig) | 0.2 mg | ¥3,244 | ![]() |
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Takara | M230 | Anti-Mouse Irx3, Polyclonal | 0.2 mg | ¥3,275 | ![]() |
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Takara | M231 | Anti-Human Crx, Polyclonal | 0.2 mg | ¥3,244 | ![]() |
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Antibodies to Neural Progenitor Cells: Bf1, Crx, Emx1, Irx3, L7/Pcp2, Otp, Otx2 and Rx |
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Immunohistochemical Staining with Polyclonal Antibody to Mouse Otx2 (Cat. No. M198) and DAPI. Tissue: Embryonic mouse, day 10 (E10), forebrain to midbrain. Fixation: 4% paraformaldehyde, 6 h. Permeabilization: 0.3% Triton X-100 in PBS. Blocking: 2% skim milk in PBS. Blue, DAPI stain; red, anti-Otx2 antibody. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Pluripotent Cell Detection酶试剂盒Takara Clontech
上海金畔生物科技有限公司代理Takara Clontech酶试剂盒全线产品,欢迎访问官网了解更多产品信息和订购。
Pluripotent Cell Detection | ||||||
品牌 | Code No. | 产品名称 | 包装量 | 价格(元) | 说明书 | 数量 |
Cellartis | Y20010 | hES-Cellect™ | 150 μg | ¥6,395 | ![]() |
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检测多能性细胞用抗体 (Monoclonal Antibodies for Detecting Pluripotent Cells) |
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Human Stem Cell Markers酶试剂盒Takara Clontech
上海金畔生物科技有限公司代理Takara Clontech酶试剂盒全线产品,欢迎访问官网了解更多产品信息和订购。
Human Stem Cell Markers | ||||||
品牌 | Code No. | 产品名称 | 包装量 | 价格(元) | 说明书 | 数量 |
Takara | M221 | Anti-Human Oct4, Monoclonal | 0.1 mg | Inquire | ![]() |
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Takara | M222 | Anti-Human Lin28, Monoclonal | 0.1 mg | Inquire | ![]() |
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Takara | M223 | Anti-Human Sox2, Monoclonal | 0.1 mg | Inquire | ![]() |
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人干细胞标志物抗体 (Human Stem Cell Markers) |
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参考文献: | |||||
Yu, J. et al. (2007) Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. Science 318:1917–1920. | |||||
iPS Cell to Endothelial Cell Differentiation System酶试剂盒Takara Clontech
上海金畔生物科技有限公司代理Takara Clontech酶试剂盒全线产品,欢迎访问官网了解更多产品信息和订购。
iPS Cell to Endothelial Cell Differentiation System | ||||||
品牌 | Code No. | 产品名称 | 包装量 | 价格(元) | 说明书 | 数量 |
Cellartis | Y50300 | MiraCell® iPS Cell to Endothelial Cell Differentiation Kit | 1 Kit | ¥13,273 | ![]() |
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Cellartis | Y50301 | MiraCell® iPS Cell to Endothelial Cell Differentiation Kit with DEF-CS™ 500 Culture System | 1 Kit | ¥18,627 | ![]() |
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Cellartis | Y50053 | MiraCell® EC Culture Medium | 500 ml | ¥4,148 | ![]() |
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iPS向血管内皮细胞定向分化系统 (iPS Cell to Endothelial Cell Differentiation System) |
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MiraCell® iPS Cell to Endothelial Cell Differentiation Kit with DEF-CS™ 500 Culture System,Y50301 | |||||||||||
· MiraCell® iPS Cell to Endothelial Cell Differentiation Kit,Y50300 · Cellartis® DEF-CS™ 500 Culture System,Y30010 |
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■ 实验例 | |||||||||||
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图1. 3种人iPS细胞株使用MiraCell iPS Cell to Endothelial Cell Differentiation Kit分化诱导,获得的血管内皮细胞的形态比较(A)和标志蛋白质(B)比较。 | |||||||||||
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图2. 3种人iPS细胞株使用MiraCell iPS Cell to Endothelial Cell Differentiation Kit分化诱导,获得的血管内皮细胞继续使用MiraCell EC Culture Medium维持培养,可长时间维持高纯度。()内的数值表示Day32的细胞扩大倍数,以Day8的细胞数为1。 | |||||||||||
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图3. 冷冻保存的、使用MiraCell iPS Cell to Endothelial Cell Differentiation Kit分化诱导的血管内皮细胞(Day16),在解冻复苏培养Day8后的小管形成实验结果。 | |||||||||||
产品详情请点击:http://catalog.takara-bio.co.jp/product/basic_info.php?unitid=U100009383 | |||||||||||
Human iPS Cell Editing Systems酶试剂盒Takara Clontech
上海金畔生物科技有限公司代理Takara Clontech酶试剂盒全线产品,欢迎访问官网了解更多产品信息和订购。
Human iPS Cell Editing Systems | ||||||
品牌 | Code No. | 产品名称 | 包装量 | 价格(元) | 说明书 | 数量 |
Cellartis | Y30021 | Cellartis iPSC Single-Cell Cloning DEF-CS™ Culture Media Kit | 1 kit | ¥7,860 | ![]() |
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从开始到结束:人iPS细胞基因编辑和单细胞克隆系统 Start-To-Finish hiPSC Editing and Single-Cell-Cloning Systems |
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■ 产品应用 | |||||||||||||||||||
· CRISPR/Cas9-mediated gene editing of hiPSCs · Single-cell cloning of hiPSCs · Disease modeling |
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■ 参考文献 | |||||||||||||||||||
Asplund, A. et al. One Standardized Differentiation Procedure Robustly Generates Homogenous Hepatocyte Cultures Displaying Metabolic Diversity from a Large Panel of Human Pluripotent Stem Cells. Stem Cell Rev. Reports 12, 90-104 (2016). Jinek, M. et al. A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity. Science 337, (2012). Kim, S., Kim, D., Cho, S. W., Kim, J. & Kim, J.-S. Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins. Genome Res. 24, 1012-9 (2014). Sander, J.-D. & Joung, J.-K. CRISPR-Cas9 systems for genomic editing, regulation and targeting. Nat. Biotechnol. 32, 347-55 (2014). |
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■ 产品详情请点击:![]() |
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Human induced pluripotent stem cells (hiPSCs) growing in DEF-CS medium were edited with Cas9 and sgRNA specific to CD81. Pluripotency is maintained in edited human induced pluripotent stem cells (hiPSCs) that have been seeded as single cells. 12 clonal lines created from single-seeded hiPSCs exhibit high levels of Oct-4 (97-99%), TRA-1-60 (98-99%), and SSEA-4 (98-99%). | |||||||||||||||||||
(图片来源于Takara Bio USA, Inc.) | |||||||||||||||||||
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13201 | PKH26 Cell Membrane Labeling Kit, 100 uL dye, – Lumiprobe荧光染料
上海金畔生物科技有限公司代理lumiprobe荧光染料全线产品,欢迎访问官网了解更多信息
13201 | PKH26 Cell Membrane Labeling Kit, 100 uL dye,
¥2,470.00
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¥3,380.00Human iPS Cell Generation™ All-in-One Vector酶试剂盒Takara Clontech
上海金畔生物科技有限公司代理Takara Clontech酶试剂盒全线产品,欢迎访问官网了解更多产品信息和订购。
Human iPS Cell Generation™ All-in-One Vector 品牌 Code No. 产品名称 包装量 价格(元) 说明书 数量 Takara 3671 Human iPS Cell Generation™ All-in-One Vector 10 μg ¥10,014 收藏产品 加入购物车无标题文档 □ 浓度 0.5 μg/μl □ 贮存溶液 Tris-HCl (pH 8.0) 10 mM EDTA 1 mM Limited Use Label License: [L44] ■ 制品说明 本制品是制备Human iPS细胞诱导用逆转录病毒所使用的载体。在高效、高表达逆转录病毒载体pDON-5 DNA(Code No.: 3658)上,插入了iPS细胞诱导用基因OCT3/4和SOX2、KLF4、LIN28和NANOG,各基因以Thosea asigna virus 的2A*1序列连接在一个载体上。本载体与Retrovirus Packaging Kit Ampho(Code No.: 6161)中的pGP Vector以及pE-ampho Vector共转染至逆转录病毒制备用细胞G3T-hi(Code No.: 6163)后,可以制备iPS诱导用逆转录病毒。如此制备的重组逆转录病毒由于和RetroNectin®(Recombinant Human Fibronectin Fragment)(Code No.: T100A/B)有高亲和性,所以在向目的细胞中导入基因时,使用经RetroNectin®处理后的培养皿或平板,可以得到较高的基因导入率,有效提高iPS细胞的诱导效率。
*1 5种基因转录在同一个mRNA上,翻译时在2A序列的特定位点被分割,使5种蛋白质可以等量表达。
■ 保存 -20℃。 ■ 各载体的遗传信息 基因名 GenBank Accession No. OCT4 NM_002701.4 SOX2 NM_003106.2 KLF4 NM_004235.3*2 LIN28 NM_024674.4 NANOG NM_024865.2 *2 更新的NM_004235.4中,CDS的5’端追加了对应9个氨基酸的碱基。本制品的KLF4基因中不含N端的这9个氨基酸的序列,已经确认可以有效诱导iPS细胞。 ■ 载体图谱 23201 | PKH26 Cell Membrane Labeling Kit, 100 uL dye, – Lumiprobe荧光染料
上海金畔生物科技有限公司代理lumiprobe荧光染料全线产品,欢迎访问官网了解更多信息
23201 | PKH26 Cell Membrane Labeling Kit, 100 uL dye,
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New!SKU: Lumiprobe.23201 分类: 默认分类- 其他信息
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保存温度 -20°C
产地 美国
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