Colourimetric method for the determination of Resistant Starch
in cereal products and feeds

Principle:
(α-amylase + amyloglucosidase)
(1) Non-resistant starch + H₂O → D-glucose + maltose (trace)

(2) Aqueous ethanol wash + centrifugation to remove D-glucose +
maltose

(3) Dissolution of resistant starch pellet in KOH and neutralisation

(α-amylase + amyloglucosidase)
(4) Dissolved resistant starch + H₂O → D-glucose

(glucose oxidase)
(5) D-Glucose + H₂O + O₂ → D-gluconate + H₂O₂

(peroxidase)
(6) 2H₂O₂ + p-hydroxybenzoic acid + 4-aminoantipyrine →
quinoneimine + 4H₂O

Kit size:                           100 assays
Method:                           Spectrophotometric at 510 nm
Reaction time:                  ~ 120 min (plus overnight incubation)
Detection limit:                 2-100% of sample weight
Application examples: 
Plant materials, starch samples and other materials
Method recognition:     
AOAC (Method 2002.02), AACC (Method 32-40.01) and CODEX
(Type II Method)

Advantages

• Very cost effective
  
• All reagents stable for > 2 years after preparation
  
• Only enzymatic kit available
  
• Measures enzyme resistant starch
  
• Simple format
  
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  
• Standard included