CAS: 35061-50-8
Molecular Formula: C₁₆H₂₈N₂O₁₁
Molecular Weight: 424.4
Purity: > 95%

High purity Diacetyl-chitobiose for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Prepared from chitin.

The Alpha-D-Glucuronidase test kit is a simple, reliable and accurate method for the measurement and analysis of alpha-D-glucuronidase in various enzyme preparations. Containing and aldouronic acid substrate and an α-D-glucuronidase control.
Suitable for manual and microplate formats.

UV-method for the measurement of α-D-Glucuronidase in
various enzyme preparations

Principle:
(α-D-glucuronidase)
(1) Aldouronic acid (tri:tetra:penta) + H₂O →
β-(1,4)-D-xylo-oligosaccharides + D-glucuronic acid

(uronate dehydrogenase; UDH)
(2) D-Glucuronic acid + NAD⁺ + H₂O → D-glucarate + NADH + H⁺

Kit size:  50 assays (manual) / 200 (microplate)
Method: Spectrophotometric at 340 nm
Reaction time:  ~ 25 min
Detection limit:  17 mU/mL
Application examples: 
Enzyme preparations and other materials
Method recognition: Novel method

Advantages

• Very competitive price (cost per test)
  
• All reagents stable for > 2 years as supplied
  
• Simple format
  
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  
• Standard included
  
• Suitable for manual, microplate and auto-analyser formats

Sample Pre-treatment Format:

CAS: 20237-70-1
Molecular Formula: C₁₀H₂₀O₉
Molecular Weight: 284.3
Purity: > 95%

High purity 1,4-β-D-Xylobiitol for use in research, biochemical enzyme assays and in vitro diagnostic analysis. This compound can be used as a substrate for xylan degrading enzymes such as endo-1,4-β-xylanase or β-xylosidase. Borohydride reduced oligosaccharides can be used in the place of native oligosaccharides when performing enzymatic degradation experiments using reducing sugar assays. The native oligosaccharides cannot be used for this purpose because, being reducing sugars themselves, they generate a large ‘blank’ value in these assays.

PureCol® collagen is known as the standard of all collagens for purity (>99.9% collagen content), functionality, and the most native-like collagen available.  PureCol® is isolated from bovine hides sourced from the only controlled, closed herd in the United States.  Advanced BioMatrix’s manufacturing processes comply with stringent quality standards that have proven to yield unsurpassed lot-to-lot consistency.

PureCol® collagen is approximately 97% Type I with the remainder being comprised of Type III collagen.  It is soluble atelo-collagen. It contains high monomer content as measured by gel permeation chromatography.

This product is supplied as a lyophilized powder with 15 mg of bovine collagen.  When reconstituted with 5 ml of sterile 0.01 N HCl, a concentration of approximately 3 mg/ml is achieved.

PureCol® collagen is ideal for coating of surfaces and providing preparations of thin layers for culturing cells. PureCol®, lyophilized form is not recommended for the formation of a solid gel.  PureCol® collagen is provided in user-friendly packaging for use and storage.  This product is supplied as a sterile lyophilized powder.

Cell Attachment Bioassay

To demonstrate cell attachment, cells were seeded onto surfaces coated with the matrix product and onto negative control surfaces without the matrix product. Cells were seeded onto surfaces using serum-free DMEM media. All surfaces were blocked with a solution containing 1% BSA. Cells were then allowed to attach for one (1) hour at 37°C. The culture surfaces were washed with DPBS. The results indicate significant cell attachment with surfaces coated using the matrix product.

Without UltraPure®	With UltraPure®
Human Umbilical Vein Endothelial Cells (HUVEC) 10X
VERO Epithelial Cells 10X
Human Dermal Fibroblast Cells 10X
MDCK Epithelial Cells 10X

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