Cy5-N-羟基琥珀酰亚胺酯|Cyanine5 NHS ester|金畔生物

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Cy5-N-羟基琥珀酰亚胺酯

货号：AGF1338A-5mg
包装：5mg

  • 英文名：Cyanine5 NHS ester
  • 级别：NMR 1H (95%) and 13C， TLC， functional testing
  • 分子式：C36H42ClN3O4
  • 分子量：616.19
  • 品牌：lumiprobe
  • CAS：1032678-42-4， 350686-88-3
  • 外观：dark blue powder
产品详情质检信息

订购货号 产品名称及规格
13020 Cyanine5 NHS ester, 1 mg
23020 Cyanine5 NHS ester, 5 mg
43020 Cyanine5 NHS ester, 25 mg
53020 Cyanine5 NHS ester, 50 mg
63020 Cyanine5 NHS ester, 100 mg

在过去的几年里，CY5荧光团已经成为生命科学研究和诊断领域非常流行的分子标记物。这些荧光素的最强发射光为红光，在这个范围大多数CCD检测器具有最大检测灵敏度，而且是生物物质低背景区。染料的颜色是非常强烈的，因此在凝胶电泳中低至1nmol的量都能被肉眼观测到。CY5 NHS ester是用于标记多肽、蛋白和寡核苷酸的氨基基团的活性染料。在标记反应中该染料需要少量的有机共溶剂（如DMF，DMSO）（请参照我们的推荐手册以获得更多帮助信息）。对于可溶性蛋白、各种各样的多肽和寡核苷酸而言，这种染料是非常理想而且成本低廉的。该染料在有机溶剂对小分子物质的标记也是非常有效的。对于更多的精细目标物，如易降解蛋白，如果DMF或DMSO会对其有影响，可以考虑water-soluble Cy5 NHS ester（水溶性Cy5 NHS ester染料），因为它们不需要有机溶剂助溶，而且具有和荧光染料类似的性质。

CY5荧光团可以与很多的仪器如荧光显微镜、成像仪、扫描仪、荧光分析仪等相兼容。许多的CY5衍生物——CY5 NHS ester可以替代Alexa Fluor 647和 DyLight 649等活化酯（activated esters）。 

Cy5-N-羟基琥珀酰亚胺酯|Cyanine5 NHS ester|金畔生物

推荐手册
氨基分子的NHS酯标记（请联系金畔生物获取）
Cy® is a trademark of GE Healthcare.
 

S" style="color: rgb(61, 61, 61);">General properties

Appearance: dark blue powder
Molecular weight: 616.19
Molecular formula:

C36H42ClN3O4
CAS number: 1032678-42-4, 350686-88-3
Solubility: good in polar (DMSO, DMF) and chlorinated (DCM, chlroform) organic solvents, low solubility in water
Quality control: NMR 1H (95%) and 13C, TLC, functional testing
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Spectral properties
Excitation maximum, nm: 646
Extinction coefficient at excitation maximum, Lmol-1cm-1: 250000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.2

 

Product citations

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