分类目录归档：同仁化学试剂盒

同仁化学试剂盒

同仁化学Hampton蛋白结晶试剂盒NDSB-221/HR2-791| 日本DOJINDO

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Products > Optimize Reagents > Optimize – Solubilizing Agents (NDSB) > NDSB-221

NDSB-221

Hampton蛋白结晶试剂盒NDSB-221/HR2-791

CAT NO

HR2-791

NAME

NDSB-221

DESCRIPTION

5 grams

PRICE

$58.00

支持材料

Hampton蛋白结晶试剂盒NDSB-221/HR2-791 HR2-791 NDSB-221 User GuideHampton蛋白结晶试剂盒NDSB-221/HR2-791 HR2-791 NDSB-221 SDS

应用

  • Crystallization grade NDSB-221 for formulating screens or for optimization
  • 结晶级 NDSB-221 用于配制筛选或优化

特征

  • Efficiently solubilizes proteins at non-denaturing concentrations
  • Useful in preventing protein aggregation
  • Non detergent sulfobetaines (NDSB) are used for protein folding, renaturation and crystallization
  • Protein specific additive for solubility and stability
  • 在非变性浓度下有效溶解蛋白质
    可用于防止蛋白质聚集
    非洗涤剂磺基甜菜碱 (NDSB) 用于蛋白质折叠、复性和结晶
    用于溶解性和稳定性的蛋白质特定添加剂

描述

The NDSB are a group of zwitterionic compounds that can reduce aggregation and aid in refolding proteins found in inclusion bodies and bacterial expression systems.

Non detergent sulfobetaines have a sulfobetaine hydrophilic group and a short hydrophobic group that cannot aggregate to form micelles, therefore NDSB’s are not considered detergents. NDSB increase the extraction yield (up to 30%) of membrane, nuclear and cyto-skeletal associated proteins. The short hydrophobic groups combined with the charge neutralization of the sulfobetaine group results in higher yields of membrane proteins. NDSB have been used in refolding and renaturation of chemically and thermally denatured proteins.

NDSB 是一组两性离子化合物，可以减少聚集并帮助重新折叠包涵体和细菌表达系统中发现的蛋白质。

非洗涤剂磺基甜菜碱有一个磺基甜菜碱亲水基团和一个不能聚集形成胶束的短疏水基团，因此 NDSB 不被视为洗涤剂。 NDSB 可提高膜、核和细胞骨架相关蛋白的提取率（高达 30%）。 短疏水基团与磺基甜菜碱基团的电荷中和相结合，导致膜蛋白产量更高。 NDSB 已用于化学和热变性蛋白质的重折叠和复性。

Typical useful NDSB concentration in protein sample is 0.5-1.0 M.

NDSB-221
Synonyms: Non-detergent sulfobetaine 221 or 3-(1-Methylpiperidinium)-1-propane Sulfonate
C9H19NO3S
Mr 221.34
Purity: ≥99.0%
Hygroscopic
Store at room temperature (+20 degrees Celsius)

HR2-791参考

1. Goldberg, M.E., et al. 1996. Folding & Design 1, 21.

2. Protein crystallography with non detergent sulfobetaines. L. Vuillard et al. J Cryst Growth (1996) 168 150-15.

3. Vuillard L, Rabilloud T, Goldberg ME, Eur J Biochem 1998 256(1):128-135. (protein folding).

同仁化学Hampton蛋白结晶试剂盒Polyethylene glycol 200/HR2-601| 日本DOJINDO

发表于由

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品，欢迎访问官网了解更多信息

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等，我们将竭诚为您服务，欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Polymers > Polyethylene glycol 200

Polyethylene glycol 200

Hampton蛋白结晶试剂盒Polyethylene glycol 200/HR2-601

CAT NO

HR2-601

NAME

100% Polyethylene glycol 200

DESCRIPTION

200 mL

PRICE

$42.00

支持材料

Hampton蛋白结晶试剂盒Polyethylene glycol 200/HR2-601 HR2-601 Polyethylene glycol 200 SDS

应用

  • Crystallization grade Polyethylene glycol 200 for formulating screens or for optimization
  • 结晶级聚乙二醇 200，用于配制筛网或优化

描述Polyethylene glycol 200

Synonyms: PEG 200
H(OCH2CH2)nOH
Average Formula weight (Mr) 200 (190-210)
CAS Number [25322-68-3]
EC Number 500-038-2
Merck 14,7568
RTECS TQ3600000
MDL Number MFCD00081839
PubChem Substance ID 24898381
Melting Point -65°C (lit.)
Density 1.1239 g/mL at 20°C
Vapor pressure <0.01 mm Hg at 20°C
Viscosity 4.3 cSt at 20°C
Refractive index N20/D 1.459 (lit.)
Aldehyde: None detected
Peroxide: None detected

Measured Conductivity Range: 2.5 – 9.7 µS/cm at 25°C
Measured Refractive Index Range: 1.46008 – 1.46124 at 20°C

同义词：PEG 200
H(OCH2CH2)nOH
平均配方重量 (Mr) 200 (190-210)
CAS 编号 [25322-68-3]
欧盟编号 500-038-2
默克 14,7568
RTECS TQ3600000
MDL 编号 MFCD00081839
PubChem 物质 ID 24898381
熔点 -65°C (lit.)
密度 1.1239 g/mL 在 20°C
蒸气压 <0.01 mm Hg at 20°C
粘度 4.3 cSt 在 20°C
折射率 N20/D 1.459 (lit.)
醛：未检测到
过氧化物：未检测到

测得的电导率范围：25°C 时为 2.5 – 9.7 µS/cm
测得的折射率范围：20°C 时为 1.46008 – 1.46124

Polyethylene glycol 200

参考

1. Crystallization and Preliminary Structural Studies of a Chorismate Mutase Catalytic Antibody Complexed With a Transition State Analog. Haynes, MR; Stura, EA; Hilvert, D; Wilson, IA. Proteins 18, 198- 200, 1994.

1. 与过渡态类似物复合的分支酸变位酶催化抗体的结晶和初步结构研究。 海恩斯先生； 斯图拉，EA； 希尔弗特，D； 威尔逊，IA。 蛋白质 18、198-200、1994。

同仁化学Hampton蛋白结晶试剂盒NDSB-256/HR2-705| 日本DOJINDO

发表于由

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品，欢迎访问官网了解更多信息

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等，我们将竭诚为您服务，欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Solubilizing Agents (NDSB) > NDSB-256

NDSB-256

Hampton蛋白结晶试剂盒NDSB-256/HR2-705

CAT NO

HR2-705

NAME

NDSB-256

DESCRIPTION

5 grams

PRICE

$57.00

支持材料

Hampton蛋白结晶试剂盒NDSB-256/HR2-705 HR2-705 NDSB-256 User GuideHampton蛋白结晶试剂盒NDSB-256/HR2-705 HR2-705 NDSB-256 SDS

应用

  • Crystallization grade NDSB-256 for formulating screens or for optimization
  • 结晶级 NDSB-256 用于配制筛选或优化

特征

  • Efficiently solubilizes proteins at non-denaturing concentrations
  • Useful in preventing protein aggregation
  • Non detergent sulfobetaines (NDSB) are used for protein folding, renaturation and crystallization
  • Protein specific additive for solubility and stability
  • 在非变性浓度下有效溶解蛋白质
    可用于防止蛋白质聚集
    非洗涤剂磺基甜菜碱 (NDSB) 用于蛋白质折叠、复性和结晶
    用于溶解性和稳定性的蛋白质特定添加剂

描述

The NDSB are a group of zwitterionic compounds that can reduce aggregation and aid in refolding proteins found in inclusion bodies and bacterial expression systems.

Non detergent sulfobetaines have a sulfobetaine hydrophilic group and a short hydrophobic group that cannot aggregate to form micelles, therefore NDSB’s are not considered detergents. NDSB increase the extraction yield (up to 30%) of membrane, nuclear and cyto-skeletal associated proteins. The short hydrophobic groups combined with the charge neutralization of the sulfobetaine group results in higher yields of membrane proteins. NDSB have been used in refolding and renaturation of chemically and thermally denatured proteins.

NDSB 是一组两性离子化合物，可以减少聚集并帮助重新折叠包含体和细菌表达系统中发现的蛋白质。

非洗涤剂磺基甜菜碱有一个磺基甜菜碱亲水基团和一个不能聚集形成胶束的短疏水基团，因此 NDSB 不被视为洗涤剂。 NDSB 可提高膜、核和细胞骨架相关蛋白的提取率（高达 30%）。 短疏水基团与磺基甜菜碱基团的电荷中和相结合，导致膜蛋白产量更高。 NDSB 已用于化学和热变性蛋白质的重折叠和复性。

Typical useful NDSB concentration in protein sample is 0.5-1.0 M.

NDSB-256
Synonyms: Dimethylbenzylammonium propane sulfonate
C12H19NO3S
Mr 257.37
Purity: ≥99% by TLC
Hygroscopic
Store at room temperature (+20 degrees Celsius)

HR2-705

参考

1. Vuillard, L., et al, J. Biochem., 305, 337 (1995).

2. A nondetergent sulfobetaine prevents protein aggregation in microcalorimetric studies. Collins et al, Notes & Tips / Anal. Biochem. 352 (2006) 299-301.

同仁化学Hampton蛋白结晶试剂盒18 mm Mounted CryoLoop™ – 20 micron/HR4-932/HR4-945/HR4-918| 日本DOJINDO

发表于由

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品，欢迎访问官网了解更多信息

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等，我们将竭诚为您服务，欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Cryocrystallography > CryoLoops > 18 mm Mounted CryoLoop™ – 20 micron

18 mm Mounted CryoLoop™ – 20 micron

Hampton蛋白结晶试剂盒18 mm Mounted CryoLoop™ &#8211; 20 micron/HR4-932/HR4-945/HR4-918
Hampton蛋白结晶试剂盒18 mm Mounted CryoLoop™ &#8211; 20 micron/HR4-932/HR4-945/HR4-918
Hampton蛋白结晶试剂盒18 mm Mounted CryoLoop™ &#8211; 20 micron/HR4-932/HR4-945/HR4-918
Hampton蛋白结晶试剂盒18 mm Mounted CryoLoop™ &#8211; 20 micron/HR4-932/HR4-945/HR4-918
Hampton蛋白结晶试剂盒18 mm Mounted CryoLoop™ &#8211; 20 micron/HR4-932/HR4-945/HR4-918

CAT NO

HR4-932

NAME

18 mm Mounted CryoLoop – 20 micron

DESCRIPTION

0.025 – 0.05 mm – 25 pack

PRICE

$74.00

CAT NO

HR4-945

NAME

18 mm Mounted CryoLoop – 20 micron

DESCRIPTION

0.05 – 0.1 mm – 25 pack

PRICE

$74.00

CAT NO

HR4-947

NAME

18 mm Mounted CryoLoop – 20 micron

DESCRIPTION

0.1 – 0.2 mm – 25 pack

PRICE

$74.00

CAT NO

HR4-970

NAME

18 mm Mounted CryoLoop – 20 micron

DESCRIPTION

0.2 – 0.3 mm – 25 pack

PRICE

$74.00

CAT NO

HR4-971

NAME

18 mm Mounted CryoLoop – 20 micron

DESCRIPTION

0.3 – 0.4 mm – 25 pack

PRICE

$74.00

CAT NO

HR4-972

NAME

18 mm Mounted CryoLoop – 20 micron

DESCRIPTION

0.4 – 0.5 mm – 25 pack

PRICE

$74.00

CAT NO

HR4-973

NAME

18 mm Mounted CryoLoop – 20 micron

DESCRIPTION

0.5 – 0.7 mm – 25 pack

PRICE

$74.00

CAT NO

HR4-974

NAME

18 mm Mounted CryoLoop – 20 micron

DESCRIPTION

0.7 – 1.0 mm – 25 pack

PRICE

$74.00

CAT NO

HR4-918

NAME

18 mm Mounted CryoLoop – 20 micron

DESCRIPTION

Sampler – 30 pack (5 of each diameter size 0.1 – 1.0 mm)

PRICE

$86.00

应用

  • Cryocrystallography
  • 低温晶体学

特征

  • Ready to use 18 mm length MicroTube with CryoLoop
  • Synthetic CryoLoop on stainless steel MicroTube
  • Complete range of loop diameters from 0.025 – 1.0 mm
  • Compatible with CrystalCap, CrystalCap ALS, CrystalCap ALS HT and CrystalCap Spine HT
  • 即用型 18 mm 长 MicroTube 和 CryoLoop
    不锈钢 MicroTube 上的合成 CryoLoop
    从 0.025 – 1.0 mm 的完整范围的环直径
    与 CrystalCap、CrystalCap ALS、CrystalCap ALS HT 和 CrystalCap Spine HT 兼容

描述

18 mm Mounted CryoLoops with 20 micron diameter nylon. Nylon loops are staked to hollow, stainless steel MicroTubes™ that are used to mount, freeze, and secure the crystal during cryocrystallographic procedures and X-ray data collection. The MicroTube on the 18 mm Mounted CryoLoops is 18 mm in length and ready to insert into CrystalCap Magnetic and CrystalCap HT. The 20 micron diameter nylon loops show minimal diffraction, are thin for fast cryo cooling of the crystal, strong, and aerodynamic.

For use with CrystalCap, CrystalCap ALS, CrystalCap ALS HT and CrystalCap SPINE HT systems. Not compatible with the CrystalCap Copper, CrystalCap Copper HT, or CrystalCap ALS HT.

18 毫米安装的 CryoLoops，带有 20 微米直径的尼龙。 尼龙环固定在空心不锈钢 MicroTubes™ 上，用于在低温晶体学程序和 X 射线数据收集过程中安装、冷冻和固定晶体。 安装在 18 mm CryoLoops 上的 MicroTube 长 18 mm，可插入 CrystalCap Magnetic 和 CrystalCap HT。 直径为 20 微米的尼龙环显示出最小的衍射，很薄，可快速冷却晶体，坚固且符合空气动力学。

用于 CrystalCap、CrystalCap ALS、CrystalCap ALS HT 和 CrystalCap SPINE HT 系统。 与 CrystalCap Copper、CrystalCap Copper HT 或 CrystalCap ALS HT 不兼容。

相关项目

  • Loctite® Easy-Squeeze Super Glue Gel
  • CrystalCap™
  • CrystalCap™ SPINE HT
  • Epoxy
  • CrystalCap™ ALS

同仁化学Hampton蛋白结晶试剂盒Mounted CryoLoop™ – 20 micron/HR4-963/HR4-959/HR4-957| 日本DOJINDO

发表于由

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品，欢迎访问官网了解更多信息

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等，我们将竭诚为您服务，欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Cryocrystallography > CryoLoops > Mounted CryoLoop™ – 20 micron

Mounted CryoLoop™ – 20 micron

应用

  • Cryocrystallography
  • 低温晶体学

特征

  • Synthetic CryoLoop on stainless steel MicroTube
  • MicroTube specially engineered with EasySnap notches
  • Complete range of loop diameters from 0.025 – 1.0 mm
  • Compatible with all CrystalCap Systems
  • 不锈钢 MicroTube 上的合成 CryoLoop
    MicroTube 特别设计，带有 EasySnap 凹槽
    从 0.025 – 1.0 mm 的完整范围的环直径
    与所有 CrystalCap 系统兼容

描述

Note: If you are using the CrystalCap, CrystalCap ALS, CrystalCap ALS HT, or CrystalCap SPINE HT, save time and money by using the ready to mount 18 mm Mounted CryoLoop.

Mounted CryoLoops with 20 micron diameter nylon. These nylon loops are bonded to hollow, stainless steel MicroTubes™ that are used to mount, freeze, and secure the crystal during cryocrystallographic procedures and X-ray data collection. The MicroTube is 24 mm in length and is specially engineered with EasySnap™ notches at the 10, 12, 14, 18, and 21 mm measures. To obtain the desired length of MicroTube, simply snap the MicroTube at the desired length and secure to the CrystalCap using epoxy or similar adhesive. These nylon loops show minimal diffraction, are thin for fast freezing, strong, and aerodynamic..

Most data collection geometries prefer or require a 22 mm length between the base of the cap and the beam. For a 22 mm overall length using the CrystalCap, CrystalCap ALS, CrystalCap ALS HT and CrystalCap SPINE HT, snap the MicroTube at the second notch from the bottom, creating an 18 mm MicroTube lenghth and secure to the CrystalCap using epoxy or similar adhesive. For a 22 mm over length using the CrystalCap Copper, CrystalCap Copper HT, CrystalCap ALS Copper HT snap the MicroTube at the fifth notch from the bottom (notch closest to the CryoLoop) and secure to the CrystalCap using epoxy or similar adhesive.

注意：如果您使用的是 CrystalCap、CrystalCap ALS、CrystalCap ALS HT 或 CrystalCap SPINE HT，请使用可安装的 18 毫米已安装 CryoLoop 来节省时间和金钱。

安装有 20 微米直径尼龙的 CryoLoops。这些尼龙环与中空不锈钢 MicroTubes™ 粘合，用于在低温晶体学程序和 X 射线数据收集过程中安装、冷冻和固定晶体。 MicroTube 的长度为 24 毫米，专门设计了 10、12、14、18 和 21 毫米尺寸的 EasySnap™ 凹槽。要获得所需长度的 MicroTube，只需将 MicroTube 卡入所需长度并使用环氧树脂或类似粘合剂固定到 CrystalCap。这些尼龙环显示出最小的衍射，很薄，可以快速冷冻，坚固且符合空气动力学。

大多数数据收集几何结构更喜欢或要求帽底和梁之间有 22 毫米的长度。对于使用 CrystalCap、CrystalCap ALS、CrystalCap ALS HT 和 CrystalCap SPINE HT 的 22 mm 总长度，将 MicroTube 从底部的第二个凹口处卡入，形成 18 mm 的 MicroTube 长度并使用环氧树脂或类似粘合剂固定到 CrystalCap。对于 22 mm 以上的长度，使用 CrystalCap Copper、CrystalCap Copper HT、CrystalCap ALS Copper HT 将 MicroTube 卡在从底部算起的第五个槽口（最靠近 CryoLoop 的槽口），并使用环氧树脂或类似粘合剂固定到 CrystalCap。

HR4-965 Mounted CryoLoop 0.7 – 1.0 mm

Hampton蛋白结晶试剂盒Mounted CryoLoop™ &#8211; 20 micron/HR4-963/HR4-959/HR4-957
Hampton蛋白结晶试剂盒Mounted CryoLoop™ &#8211; 20 micron/HR4-963/HR4-959/HR4-957
Hampton蛋白结晶试剂盒Mounted CryoLoop™ &#8211; 20 micron/HR4-963/HR4-959/HR4-957
Hampton蛋白结晶试剂盒Mounted CryoLoop™ &#8211; 20 micron/HR4-963/HR4-959/HR4-957
Hampton蛋白结晶试剂盒Mounted CryoLoop™ &#8211; 20 micron/HR4-963/HR4-959/HR4-957

CAT NO

HR4-313

NAME

Mounted CryoLoop – 20 micron

DESCRIPTION

0.025 – 0.05 mm – 25 pack

PRICE

$84.00

CAT NO

HR4-625

NAME

Mounted CryoLoop – 20 micron

DESCRIPTION

0.05 – 0.1 mm – 25 pack

PRICE

$84.00

CAT NO

HR4-955

NAME

Mounted CryoLoop – 20 micron

DESCRIPTION

0.1 – 0.2 mm – 25 pack

PRICE

$84.00CAT NO

HR4-957

NAME

Mounted CryoLoop – 20 micron

DESCRIPTION

0.2 – 0.3 mm – 25 pack

PRICE

$84.00

CAT NO

HR4-959

NAME

Mounted CryoLoop – 20 micron

DESCRIPTION

0.3 – 0.4 mm – 25 pack

PRICE

$84.00

CAT NO

HR4-961

NAME

Mounted CryoLoop – 20 micron

DESCRIPTION

0.4 – 0.5 mm – 25 pack

PRICE

$84.00

CAT NO

HR4-963

NAME

Mounted CryoLoop – 20 micron

DESCRIPTION

0.5 – 0.7 mm – 25 pack

PRICE

$84.00

CAT NO

HR4-965

NAME

Mounted CryoLoop – 20 micron

DESCRIPTION

0.7 – 1.0 mm – 25 pack

PRICE

$84.00

CAT NO

HR4-953

NAME

Mounted CryoLoop – 20 micron

DESCRIPTION

Sampler – 30 pack (5 of each diameter size 0.1 – 1.0 mm)

PRICE

$103.00

参考

1. FeeM, an N-acyl amino acid synthase from an uncultured soil microbe: Structure, mechanism, and acyl carrier protein binding. Ryan M. Van Wagoner and Jon Clardy. Structure, 2006, Volume 14, Number 9, 1425-1435.

1. FeeM，一种来自未培养土壤微生物的 N-酰基氨基酸合酶：结构、机制和酰基载体蛋白结合。 瑞安 M. 范瓦格纳和乔恩克拉迪。 结构，2006 年，第 14 卷，第 9 期，1425-1435。