Shipping Temperature: Ambient

Storage Temperature: 4oC

Formulation: In 3.2 M ammonium sulphate

Physical Form: Suspension

Stability: > 4 years at 4oC

Enzyme Activity: Isoamylase

EC Number: 3.2.1.68

CAZy Family: GH13

CAS Number: 9067-73-6

Synonyms: isoamylase; glycogen 6-alpha-D-glucanohydrolase

Source: Flavobacterium odoratum

Molecular Weight: 83,000

Concentration: Supplied at ~ 200 U/mL

Expression: Purified from Flavobacterium odoratum

Specificity: Hydrolysis of (1,6)-α-D-glucosidic branch linkages in glycogen, amylopectin and their β-limit dextrins.

Specific Activity: ~ 140 U/mg (40oC, pH 6.0 with 1 mM CaCl2 on oyster glycogen)

Unit Definition: One Unit of isoamylase activity is defined as the amount of enzyme required to release one µmole of glucose reducing sugar equivalents per minute from oyster glycogen (10 mg/mL) in MES buffer (100 mM, pH 6.0) plus 1 mM CaCl2 at 40oC.

Temperature Optima: 50oC

pH Optima: 6

Application examples: Applications in carbohydrate research and in the food and feeds, and cereals industry.

Method recognition: AOAC Method 2000.11 and GB Standard 5009.245-2016