The NEBNext Ultra II FS DNA Module has been optimized to convert 100 pg – 0.5 μg of intact DNA to fragmented, end-repaired DNA having 5´ phosphorylated, 3´ dA-tailed ends. The module is optimized for use

with the NEBNext Ultra II Ligation Module (NEB #E7595), and is part of the Ultra II FS DNA workflow.

For Illumina library construction, the NEBNext Ultra II FS DNA Module is designed for use with the 

following:

• NEBNext Ultra II Ligation Module (NEB #E7595) 
• NEBNext Ultra II Q5® Master Mix (NEB #M0544) 
• NEBNext Oligos for Illumina (NEB #E7335, #E7500, #E7710, #E7730, #E6609, #E7600 #E7535, 
• #E7350) 

TE Buffer

NEBNext® Ultra™ II FS Enzyme Mix

NEBNext® Ultra™ II FS Reaction Buffer

-20°C

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