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凑单关联产品TOP5

NO.1.    Cell Counting Kit-8     细胞增殖毒性检测   

NO.2.    PI/RNase Staining    细胞周期检测   

NO.3.    Caspase-3 Assay Kit -Colorimetric    细胞凋亡检测  

NO.4.    ROS Assay Kit    活性氧检测

NO.5.    Calcein-AM/PI细胞双染试剂盒    活死细胞双染 

细胞凋亡是指为维持有机体内环境稳定，由基因控制的细胞自主的有序的死亡。正常情况下任何细胞在形成过程中发生的异常都会通过凋亡消除。例如体内的癌细胞增长为肿瘤的过程会受细胞凋亡的引导而被抑制。然而在抑癌基因p53出现问题时，凋亡就不会诱导发生，从而导致癌细胞的不断增长。细胞凋亡可以通过细胞形态的变化或生物化学的变化来检测。目前常用的指标有caspase活性变化、DNA碎片、磷脂酰丝氨酸的外翻等。

Annexin V染色的细胞可以用于检测细胞凋亡早期的细胞膜变化。在细胞凋亡早期，膜磷脂酰丝氨酸由脂膜内侧翻向外侧。Annexin V是一种分子量为35-36kD的Ca²⁺依赖性磷脂结合蛋白，与磷脂酰丝氨酸有高度亲和力，可通过细胞外侧暴露的磷脂酰丝氨酸与凋亡早期细胞的胞膜特异性结合，因此Annexin V 被作为检测细胞早期凋亡的灵敏指标之一。用绿色荧光FITC标记的Annexin V通过流式细胞仪或荧光显微镜可以检测到细胞凋亡的发生。碘化丙啶 (Propidium Iodide, PI) 是一种核酸染料，PI只能透过凋亡晚期和死细胞的细胞膜，因此Annexin V和PI结合使用，可以区分凋亡早晚期的细胞及死细胞。

Normal Cell                         Early stages of apoptosis                   Late stages of apoptosis

-流式细胞仪检测

1. 加样到流式细胞仪检测，激发波长Ex = 488 nm；发射波长Em = 530 nm。

2. Annexin V-FITC的绿色荧光通过FITC通道 (FL1) 检测；PI的红色荧光通过PI 通道 (FL2或FL3) 检测 (建议使用FL3)。

-荧光显微镜检测

将细胞悬液滴到玻片上，置于显微镜上使用合适的滤光片检测。* 由于EDTA会对细胞膜的特定部位有损伤，建议使用不含EDTA的胰酶消化贴壁细胞。

所需的设备和材料

– 样品和诱导剂

– PBS、超纯水

– 合适量程的移液器

– 细胞培养用6，12，24，96孔板

– 流式细胞仪或荧光显微镜。

* 最大激发/发射波长  Annexin V, FITC: 494 nm/518 nm；PI: 535 nm/617 nm

溶液制备

1×Annexin V Binding solution：将10×Annexin V Binding Buffer用超纯水稀释10倍。

稳定性

试剂盒在0-5℃下可以保存6个月，请避光保存Annexin V, FITC结合物。

＊实验实例及操作步骤请参考中文说明书

1. A Novel Allosteric Inhibitor of Phosphoglycerate Mutase 1 Suppresses Growth and Metastasis of Non-Small-Cell Lung Cancer,

Cell Metabolism, 2019, 30, 1-13

2. Tumor Microenvironment Activable Self-Assembled DNA Hybrids for pH and Redox Dual-Responsive Chemotherapy/PDT

Treatment of Hepatocellular Carcinoma, Advanced Science, 2017, 4(4), 1600460

3. Biogenic (R)-(+)-Lipoic Acid Only Constructed Cross-LinkedVesicles with Synergistic Anticancer Potency，

Advanced Functional Materials, 2018, 1806567

4. Anti-mitotic chemotherapeutics promote apoptosis through TL1A-activated death receptor 3 in cancer cells,

Cell Research, 2018, 28, 544-555

5. Bacteria-Driven Hypoxia Targeting for Combined Biotherapy and Photothermal Therapy, ACS Nano, 2018, 12(6), 5995-6005

6. Nanoenzyme-Augmented Cancer Sonodynamic Therapy by Catalytic Tumor Oxygenation, ACS Nano, 2018, 12, 3780-3795

7. Tumor-Specific Multiple Stimuli-Activated Dendrimeric Nanoassemblies with Metabolic Blockade Surmount Chemotherapy

Resistance, ACS Nano, 2017, 11(1), 416-429

8. Near Infrared-Guided Smart Nanocarriers for MicroRNA-Controlled Release of Doxorubicin/siRNA with Intracellular ATP as Fuel,

ACS Nano, 2016, 10(3), 3637-47

9. Noninvasive small-animal imaging of galectin-1 upregulation for predicting tumor resistance to radiotherapy,

Biomaterials, 2018, 158, 1-9

10. Cytotoxicity of guanine-based degradation products contributes to the antiproliferative activity of guanine-rich oligonucleotides,Chemical Science, 2015, 6, 3834-3836

11. A Novel Strategy through Combining iRGD Peptide with Tumor-icroenvironment-Responsive and Multistage Nanoparticles

for Deep Tumor Penetration, ACS Appl.Mater.Interfaces, 2015, 7(49), 27458-27466

12. JOSD1 inhibits mitochondrial apoptotic signalling to drive acquired chemoresistance in gynaecological cancer by stabilizing MCL1,Cell Death & Differentiation, 2020, 27, 55-70

13. The influence of printing parameters on cell survival rate and printability in microextrusion-based 3D cell printing technology,Biofabrication, 2015, 7(4), 045002

14. Downregulation of MCL-1 and upregulation of PUMA using mTOR inhibitors enhance antitumor efficacy of BH3 mimetics in triple-negative breast cancer, Cell Death & Disease, 2018, 9, 137

15. Mutations in the P10 region of procaspase-8 lead to chemotherapy resistance in acute myeloid leukemia by impairing procaspase-8 dimerization, Cell Death & Disease, 2018, 9(5), 516

16. Preclinical evaluation of antitumor of the proteasome inhibitor MLN2238 (ixazomib) in hepatocellular carcinoma cells,Cell Death & Disease, 2018, 9, 28

17. Long Noncoding RNA HCAL Facilitates the Growth and Metastasis of Hepatocellular Carcinoma by Acting as a ceRNA of LAPTM4B,Molecular Therapy: Nucleic Acids, 2017, 9, 440-451

18. TMEM74 promotes tumor cell survival by inducing autophagy via interactions with ATG16L1 and ATG9A,Cell Death & Disease, 2017, 8, e3031-3045

19. Nogo-C regulates cardiomyocyte apoptosis during mouse myocardial infarction, Cell Death & Disease, 2016, 7, e2432

20. TonEBP modulates the protective effect of taurine in ischemia-induced cytotoxicity in cardiomyocytes,Cell Death & Disease, 2015, 6, e2025