标签归档：stem

Human Stem Cell Culture System (DEF-CS, all-in-one format)酶试剂盒Takara Clontech

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上海金畔生物科技有限公司代理Takara Clontech酶试剂盒全线产品，欢迎访问官网了解更多产品信息和订购。

Human Stem Cell Culture System (DEF-CS, all-in-one format)
品牌 Code No. 产品名称 包装量 价格（元） 说明书 数量
Cellartis Y30010 Cellartis® DEF-CS™ 500 Culture System 1 Kit ￥5,979 Human Stem Cell Culture System (DEF-CS, all-in-one format) Human Stem Cell Culture System (DEF-CS, all-in-one format) Human Stem Cell Culture System (DEF-CS, all-in-one format)
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Human iPS和Human ES增殖用培养基
(DEF-CS Culture System, all-in-one format)
作为一款高端创新型的产品，Cellartis DEF-CS培养系统使得单细胞干细胞操作成为常规的操作。该产品是专门针对人类诱导干细胞（hiPS）和人类胚胎干细胞（hES）研发的高效率增殖用培养基产品体系。该产品系统为all-in-one型，包括了所有组分，客户不需要另外求购组分。该产品是成分确定的培养基产品，而且不需要饲养层。DEF-CS既可以实现单细胞培养，也可以用于传统的iPS培养模式以及大规模干细胞增殖。在使用DEF-CS培养基增殖干细胞时，几乎没有背景分化的问题，这使得细胞筛选不再必需。作为一款创新产品，利用DEF-CS培养干细胞时，可以使用酶消化法（enzymatic passaging）实现需要单细胞操作，这一特点十分有利于高通量细胞鉴别筛选（high-throughput screening）、转染（transfection）、框架接种（scaffold seeding）等。
 
■ 产品特点
· Feeder-free and chemically defined culture system for efficient expansion of human iPS cells
· Ideal system for culturing iPS cells; cells maintain pluripotency and long-term potential for self-renewal and differentiation
· Complete kit includes 500 ml basal medium, additives, and coating compound
· Robust system provides high reproducibility and stable growth
· Maintains cells in an undifferentiated state with virtually no background differentiation
 
■ 产品应用
· Scale-up and mass production of human iPS cells
· Single-cell culture of human iPS cells
· Transfection and reprogramming
· High-throughput screening
· Tissue engineering (seeding cells on a scaffold)
 
■ 产品详情请点击：Human Stem Cell Culture System (DEF-CS, all-in-one format)
 
Human Stem Cell Culture System (DEF-CS, all-in-one format)
Robust growth of human induced pluripotent stem (iPS) cells in the Cellartis DEF-CS Culture System. The number of iPS cells was quantified after being cultured for three weeks using either the Cellartis DEF-CS Culture System, a reference feeder system, or four other stem cell culture systems.
 
参考文献：
1. Sivertsson, Louise, et al. "Hepatic differentiation and maturation of human embryonic stem cells cultured in a perfused three-dimensional bioreactor." Stem cells and development 22.4 (2012): 581-594.
2. Hanson, Charles, et al. "Transplantation of human embryonic stem cells onto a partially wounded human cornea in vitro." Acta ophthalmologica 91.2 (2013): 127-130.
3. Norrman, Karin, et al. "Distinct gene expression signatures in human embryonic stem cells differentiated towards definitive endoderm at single-cell level." Methods 59.1 (2013): 59-70.
4. Ulvestad, Maria, et al. "Drug metabolizing enzyme and transporter protein profiles of hepatocytes derived from human embryonic and induced pluripotent stem cells." Biochemical pharmacology 86.5 (2013): 691-702.
5. Ramirez JM, et al. Side scatter intensity is highly heterogeneous in undifferentiated pluripotent stem cells and predicts clonogenic self-renewal. Stem Cells Dev.2013 Jun 15;22(12):1851-1860.
6. Borestrom, Cecilia, et al. “Footprint-free human induced pluripotent stem cells from articular cartilage with redifferentiation capacity: A first step toward a clinical-grade cell source.” Stem Cells Trans. Med. (2014) 3, 433-447.
7. Kia, Richard, et al. "MicroRNA-122: a novel hepatocyte-enriched in vitro marker of drug-induced cellular toxicity." Toxicological Sciences (2014): kfu269.
8. Valton, Julien, et al. "Efficient strategies for TALEN-mediated genome editing in mammalian cell lines."  Methods 69.2 (2014): 151-170.
9. Zandén, Carl, et al. "Stem cell responses to plasma surface modified electrospun polyurethane scaffolds." Nanomedicine: Nanotechnology, Biology and Medicine 10.5 (2014): 949-958.
10. Asplund, Annika, et al. “One Standardized Differentiation Procedure Robustly Generates Homogenous Hepatocyte Cultures Displaying? Metabolic Diversity from a Large Panel of Human Pluripotent Stem Cells” Stem Cell Rev and Rep (2015)
 
 
 
 

Human Stem Cell Derived Intestinal Epithelial Cells酶试剂盒Takara Clontech

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上海金畔生物科技有限公司代理Takara Clontech酶试剂盒全线产品，欢迎访问官网了解更多产品信息和订购。

Human Stem Cell Derived Intestinal Epithelial Cells
品牌 Code No. 产品名称 包装量 价格（元） 说明书 数量
Cellartis Y50035 Cellartis® Intestinal Epithelial Cells (from ChiPSC18) Kit 1 Kit ￥20,754 Human Stem Cell Derived Intestinal Epithelial Cells Human Stem Cell Derived Intestinal Epithelial Cells Human Stem Cell Derived Intestinal Epithelial Cells
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来源于人类iPS细胞的小肠上皮细胞
(Intestinal Epithelial Cells Derived from Human Induced Pluripotent Stem Cells)
Cellartis Intestinal Epithelial Cells (from ChiPSC18) 是来源于人iPS细胞的小肠上皮细胞，具备肠上皮样特征，包括形成紧密连接、表达绒毛蛋白villin和肠转录因子CDX2。与人结肠癌Caco-2细胞系相比，该细胞具有更高的代谢酶CYP3A4和转运蛋白PEPT1的表达，为药物代谢吸收、药物相互作用评估提供更好的预测结果。
Cellartis Intestinal Epithelial Cells (from ChiPSC18) Kit 包括1管冻存的细胞Cellartis Intestinal Epithelial Cells (from ChiPSC18)和1个试剂盒Cellartis IEC Maturation Kit。解冻的Cellartis Intestinal Epithelial Cells (from ChiPSC18)使用Cellartis IEC Maturation Kit预先培养5天后，可用于各种检测。
 
■ 产品特点
· 解冻5天内细胞形成紧密连接，解冻5-8天后可用于检测
· 细胞高表达肠上皮标志物 (villin、CDX2等)
· 与Caco-2细胞相比，拥有更高的代谢酶CYP3A4和转运蛋白PEPT1的表达
· 细胞形成一个功能性的、可渗透的屏障，可用于预测药物在体内的渗透性和吸收
 
■ 产品应用
· 药物在小肠的吸收和代谢评价
· 药物研发中的ADME分析
· 药物渗透性检测
 
■ 产品组成
· 1 vial of～4.8 x 106 cells
· 1 Cellartis IEC Maturation Kit（IEC Maturation Basal Medium；IEC Supplement A；IEC Supplement B；IEC Supplement C；IEC Supplement D）
 
Human Stem Cell Derived Intestinal Epithelial Cells
 
实验例
Human Stem Cell Derived Intestinal Epithelial Cells
图1. 紧密连接蛋白ZO-1的免疫荧光染色结果显示，解冻后第5天，hiPSC-derived intestinal epithelial cells (IEC)细胞间形成紧密连接（如闭锁小带zonula occludens）。
 
Human Stem Cell Derived Intestinal Epithelial Cells
图2. 在解冻后第7天，与Caco-2细胞相比，hiPSC-derived intestinal epithelial cells (IEC)中高表达肠上皮标志物villin和CDX2。
 
Human Stem Cell Derived Intestinal Epithelial Cells
图3. 在解冻后第7天，与Caco-2细胞相比，hiPSC-derived intestinal epithelial cells (IEC)中高表达药物代谢酶CYP3A4和药物转运蛋白PEPT1。
 
 
■ 产品详情请点击：Human Stem Cell Derived Intestinal Epithelial Cells
 
 

Human Stem Cell Markers酶试剂盒Takara Clontech

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上海金畔生物科技有限公司代理Takara Clontech酶试剂盒全线产品，欢迎访问官网了解更多产品信息和订购。

Human Stem Cell Markers
品牌 Code No. 产品名称 包装量 价格（元） 说明书 数量
Takara M221 Anti-Human Oct4, Monoclonal 0.1 mg Inquire Human Stem Cell Markers Human Stem Cell Markers Human Stem Cell Markers
Takara M222 Anti-Human Lin28, Monoclonal 0.1 mg Inquire Human Stem Cell Markers Human Stem Cell Markers Human Stem Cell Markers
Takara M223 Anti-Human Sox2, Monoclonal 0.1 mg Inquire Human Stem Cell Markers Human Stem Cell Markers Human Stem Cell Markers
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人干细胞标志物抗体
(Human Stem Cell Markers)
通过向体细胞引入“发育全能性相关因子”可以获得诱导干细胞（iPS）。例如，通过在人类体细胞内异位表达（ectopic expression）Oct4, Sox2, Lin28以及 Nanog，可以诱导细胞获取发育全能性（pluripotency）(Yu et al., 2007)。针对这些因子的抗体，可以使用在Western blot实验或是免疫组织化学实验中。
 
■ 产品应用
· Western blot analysis under reducing conditions
· Immunocytochemistry
 
Human Stem Cell Markers
 
参考文献：
Yu, J. et al. (2007) Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. Science 318:1917–1920.
 

SCREEN-WELL® Stem Cell Library Screen-Well干细胞化合物库 品牌：Enzo

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SCREEN-WELL® Stem Cell Library

Screen-Well干细胞化合物库

品牌：Enzo
CAS No.：
储存条件：-80℃
纯度：–
产品编号

(生产商编号)

 等级 规格 运输包装 零售价(RMB) 库存情况 参考值

ENZ-LIB103-0100

 – 1 Library – 咨询 – –


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动，恕不另行通知，确切详情请联系上海金畔生物科技有限公司。

Stem Cell Factor(SCF), Rat, recombinant 大鼠干细胞因子 品牌：FUJIFILM Wako

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Stem Cell Factor(SCF), Rat, recombinant

大鼠干细胞因子

品牌：FUJIFILM Wako
CAS No.：
储存条件：-20℃
纯度：–
产品编号

(生产商编号)

 等级 规格 运输包装 零售价(RMB) 库存情况 参考值

192-14601

 for Cellbiology 10ug – 4,760.00 – –


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动，恕不另行通知，确切详情请联系上海金畔生物科技有限公司。