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品牌：Cosmo Bio
CAS No.： 储存条件：-20℃ 纯度：– |
| 产品编号
(生产商编号) |
等级 | 规格 | 运输包装 | 零售价(RMB) | 库存情况 | 参考值 |
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PRX-MFL0283 |
– | 100 µl | – | 咨询 | – | – |
* 干冰运输、大包装及大批量的产品需酌情添加运输费用
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Generate full (100%) coverage of all protein coding regions in BRCA1 and BRCA2 genes
Obtain highly uniform sequencing across exon targets- 100% of base pairs in panel have coverage
greater than 20% of the mean target coverage
Maximize efficiency with a 1-day workflow that combines highly specific enrichment with library
preparation
Produce high depths of target coverage across a wide range of DNA input amounts for germline and
somatic variant calling
The NEBNext Direct BRCA1/BRCA2 Panel enriches the complete exon content of BRCA1 and BRCA2
genes for next-generation sequencing analysis. NEBNext Direct employs a unique hybridization-based
enrichment workflow that hybridizes baits directly to genomic DNA, without the need for upfront library
preparation. The BRCA1/BRCA2 panel demonstrates extremely high specificity and unmatched coverage
uniformity across a wide range of DNA inputs, allowing highly sensitive calling of germline and somatic
variants while maximizing sequencer efficiency.
Figure 1. NEBNext Direct Workflow.
NEBNext Sample Purification Beads
NEBNext Direct BRCA1/BRCA2 Baits
NEBNext Direct® Bead Prep Buffer
NEBNext Direct® Hybridization Buffer
NEBNext Direct® dA-Tailing Buffer
NEBNext Direct® Adaptor Ligation Buffer
NEBNext Direct® Cleaving Buffer
NEBNext Direct® Hybridization Wash (HW)
NEBNext Direct® Bead Wash 1
NEBNext Direct® Bead Wash 2
NEBNext Direct® 3´ Adaptor
NEBNext Direct® 5´ UMI Adaptor
NEBNext Direct® dA-Tailing Enzyme
NEBNext Direct® Ligase
NEBNext Direct® 5´ Blunting Enzyme Mix
NEBNext Direct® Cleaving Enzyme Mix
NEBNext Direct® Q5 Master Mix
NEBNext Direct® FFPE Phosphorylation Enzyme
NEBNext Direct® Streptavidin Beads 4
NEBNext Direct® Hybridization Additive -20
NEBNext Direct® 5´ Blunting Buffer 4
NEBNext Direct® 3´ Blunting Enzyme Mix