上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
NEBNext Direct® BRCA1/BRCA2 Panel 收藏
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产品优势
Generate full (100%) coverage of all protein coding regions in BRCA1 and BRCA2 genes
Obtain highly uniform sequencing across exon targets- 100% of base pairs in panel have coverage
greater than 20% of the mean target coverage
Maximize efficiency with a 1-day workflow that combines highly specific enrichment with library
preparation
Produce high depths of target coverage across a wide range of DNA input amounts for germline and
somatic variant calling
概述
The NEBNext Direct BRCA1/BRCA2 Panel enriches the complete exon content of BRCA1 and BRCA2
genes for next-generation sequencing analysis. NEBNext Direct employs a unique hybridization-based
enrichment workflow that hybridizes baits directly to genomic DNA, without the need for upfront library
preparation. The BRCA1/BRCA2 panel demonstrates extremely high specificity and unmatched coverage
uniformity across a wide range of DNA inputs, allowing highly sensitive calling of germline and somatic
variants while maximizing sequencer efficiency.
Figure 1. NEBNext Direct Workflow.
试剂盒组份
NEBNext Sample Purification Beads
NEBNext Direct BRCA1/BRCA2 Baits
NEBNext Direct® Bead Prep Buffer
NEBNext Direct® Hybridization Buffer
NEBNext Direct® dA-Tailing Buffer
NEBNext Direct® Adaptor Ligation Buffer
NEBNext Direct® Cleaving Buffer
NEBNext Direct® Hybridization Wash (HW)
NEBNext Direct® Bead Wash 1
NEBNext Direct® Bead Wash 2
NEBNext Direct® 3´ Adaptor
NEBNext Direct® 5´ UMI Adaptor
NEBNext Direct® dA-Tailing Enzyme
NEBNext Direct® Ligase
NEBNext Direct® 5´ Blunting Enzyme Mix
NEBNext Direct® Cleaving Enzyme Mix
NEBNext Direct® Q5 Master Mix
NEBNext Direct® FFPE Phosphorylation Enzyme
NEBNext Direct® Streptavidin Beads 4
NEBNext Direct® Hybridization Additive -20
NEBNext Direct® 5´ Blunting Buffer 4
NEBNext Direct® 3´ Blunting Enzyme Mix
NEBNext Direct BRCA1/BRCA2 Resources
- GRCh37hg19 Bed Files for the NEBNext Direct® BRCA1/BRCA2 Panel
- GRCh38hg38 Bed Files for the NEBNext Direct® BRCA1/BRCA2 Panel
- Protocol for NEBNext Direct BRCA1/BRCA2 Panel (E6627)