Phosphorylation plays a critical role in the regulation of many cellular functions. Common phosphorylation detection methods include expensive and often unavailable phospho-antibodies or unsafe radioactive ATP labeling. We offer pIMAGO – a novel, non-antibody, highly effective and safe product for unbiased universal detection of any phosphoprotein. Unlike antibodies, pIMAGO is capable of selective binding to a phosphorylated residue independent of amino acid sequence. This pIMAGO kit enables the detection of phosphoproteins in Western Blot format (on-membrane) using fluorescence at 550nm. The kit includes pIMAGO-biotin reagent, avidin-Fluor 550 detection reagent (for fluorescence detection at 550 nm), blocking buffer, pIMAGO buffer, washing buffer and a control phosphoprotein.