人定型内胚层细胞向肝脏细胞定向分化系统酶试剂盒Takara Clontech

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人定型内胚层细胞向肝脏细胞定向分化系统
品牌 Code No. 产品名称 包装量 价格(元) 说明书 数量
Cellartis Y30050 Cellartis® Hepatocyte Differentiation Kit 1 Kit ¥8,072 人定型内胚层细胞向肝脏细胞定向分化系统 人定型内胚层细胞向肝脏细胞定向分化系统 人定型内胚层细胞向肝脏细胞定向分化系统
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Cellartis分化获取肝细胞试剂盒
(Cellartis Hepatocyte Differentiation Kit)
The Cellartis Hepatocyte Differentiation Kit用于诱导内胚层细胞(definitive endoderm (DE))获取肝细胞。通过使用本产品获取的肝细胞表现出符合预期的代谢酶表达,从而可以用于药物代谢研究和毒理学筛选。本产品包含足够的维持培养基用于正常为期11天的实验。
The Cellartis Hepatocyte Differentiation Kit可以对广泛来源的DE细胞进行定向分化:客户自己的DE细胞,或是Cellartis提供的标准化DE细胞,或是客户利用Cellartis Definitive Endoderm Differentiation Kit获得的DE细胞。据估计,现在已经有28种发源于人类干细胞的DE细胞被成功测试可以使用本产品。特别地,如果客户是用于大规模、要求持续稳定获得肝细胞的相关用途,Cellartis推荐Definitive Endoderm Cells (from ChiPSC18),用作起始DE细胞材料,或是用作阳性对照品。如果客户要求的DE是来自于自有的样本材料(patient-specific, or disease-specific),建议使用Cellartis iPS Cell to Hepatocyte Differentiation System (Cat. No. Y30055)。
 
■ 产品特点
· Complete kit—includes four different media sets and a ready-to-use coating, sufficient for
   23-day protocol for differentiating definitive endoderm (DE) cells into hepatocytes
· Robust protocol—tested with more than 25 different stem cell lines
· Efficient differentiation—yields >90% pure hepatocytes
 
■ 关联产品
 
Code No. Product Size
Y10040 Cellartis® Definitive Endoderm Cells (from ChiPSC18) 1 Vial
Y30051 Cellartis® Hepatocyte Maintenance Medium 100 ml
 
■ 产品详情请点击:人定型内胚层细胞向肝脏细胞定向分化系统
 
人定型内胚层细胞向肝脏细胞定向分化系统
Definitive endoderm (DE) cells were differentiated into hepatocytes using the Cellartis Hepatocyte Differentiation Kit. The first field of view, labeled “Definitive Endoderm Day 7,” shows DE cells seven days after differentiation from human pluripotent cells. These DE cells became the starting material for the differentiation kit. The remaining progression from DE cells to hepatocytes shows the expected morphology over a 14-day time period. The morphological features present in the last field of view, labeled “Hepatocyte Day 21+,” continue stably past Day 21.