High purity exo-1,3-β-D-Glucanase Trichoderma sp. + β-Glucosidase (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
EC 3.2.1.58 (exo-1,3-β-Glucanase)
CAZy Family: GH55
CAS: 9073-49-8
glucan 1,3-beta-glucosidase; 3-beta-D-glucan glucohydrolase
EC 3.2.1.21 (β-Glucosidase)
CAZy Family: GH3
CAS: 9001-22-3
beta-glucosidase
exo-1,3-β-D-Glucanase: Recombinant. From Trichoderma sp.
β-Glucosidase: From Aspergillus niger.
In 3.2 M ammonium sulphate.
Activity/Concentration: exo-1,3-β-Glucanase (100 U/mL) / β-Glucosidase (20 U/mL).
Stability: > 2 years at 4oC.
DESCRIPTION
exo-1,3-β-D-Glucanase + β-Glucosidase
EC 3.2.1.58 (exo-1,3-β-D-Glucanase)
CAZy Family: GH55
CAS: 9073-49-8
EC 3.2.1.21 (β-Glucosidase)
CAZY Family: GH3
CAS: 9001-22-3
Synonyms
(exo-1,3-β-D-Glucanase): glucan 1,3-beta-glucosidase; 3-beta-D-glucan glucohydrolase (β-Glucosidase): beta-glucosidase
Form:
In 3.2 M ammonium sulphate.
Stability:
> 2 years at 4oC.
Activity:
exo-1,3-β-Glucanase: 100 U/mL
β-Glucosidase: 20 U/mL
Unit definition:
One Unit of exo-1,3-β-glucanase activity is defined as the amount of enzyme required to release one μmole of glucose reducing-sugar equivalents per minute from laminarin (10 mg/mL) in sodium acetate buffer (100 mM), pH 4.0 at 40oC.
One Unit of β-glucosidase activity is defined as the amount of enzyme required to release one μmole of p-nitrophenol per minute from 4-nitrophenyl-β-D-glucpyranoside in sodium acetate buffer (100 mM), pH 4.0 at 40oC.
Specificity:
exo-1,3-β-glucanase: Successive hydrolysis of β-D-glucose units from the non-reducing ends of (1,3)-β-D-glucans, releasing α-glucose.
β-glucosidase: Hydrolysis of terminal, non-reducing β-D-glucosyl residues with release of β-D-glucose.
Applications:
For use in the determination of (1,3)(1,4) β-glucan.
