上海金畔生物科技有限公司代理Mezazyme酶试剂盒全线产品,欢迎访问Megazyme试剂盒官网了解更多信息
Cellulase Assay Kit (CellG3 Method)
180 / 360 assays per kit
Prices exclude VAT
Content: | 180 / 360 assays per kit |
Shipping Temperature: | Ambient |
Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
Stability: | > 2 years under recommended storage conditions |
Analyte: | endo-Cellulase |
Assay Format: | Spectrophotometer, Auto-analyser |
Detection Method: | Absorbance |
Wavelength (nm): | 400 |
Signal Response: | Increase |
Limit of Detection: | 0.05 U/mL |
Reproducibility (%): | ~ 3% |
Total Assay Time: | ~ 20 min |
Application examples: | Fermentation broths, industrial enzyme preparations and biofuels research. |
Method recognition: | Novel method |
This product has been discontinued (read more).
The CellG3 assay reagent for the measurement of endo-cellulase (endo-1,4-β-glucanase) contains two components;
1) 4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-D-cellotrioside (BCNPG3) and 2) thermostable β-glucosidase. The benzylidene blocking group prevents any hydrolytic action by the β-glucosidase on BCNPG3. Incubation with an endo-cellulase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary β-glucosidase. The rate of formation of 2-chloro-4-nitrophenol is therefore directly related to the hydrolysis of BCNPG3 by the endo-cellulase. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH 9.0).
Please note that a new assay kit (K-CellG5) is now available for the measurement of endo-cellulase. The CellG5 reagent contains a cellopentaose core and exhibits vastly improved sensitivity for some cellulases. In addition, the exchange of the benzylidene blocking group in CellG3 for 3-keto-butylidene in CellG5 improves the substrate’s water solubility significantly, allowing for a reduction in the concentration of DMSO required in the assay. As DMSO is known to inhibit certain cellulases, this is another benefit in using CellG5. Megazyme now recommends the use of K-CellG5 for all assays for the measurement of endo-cellulase.
Browse more cellulase and other enzyme activity assay kits.
- Very cost effective
- All reagents stable for > 2 years after preparation
- Completely specific for cellulase (endo-1,4-glucanase). The substrate is not hydrolysed by β-glucosidase, cellbiohyrolase or any other enzymes tested
- Kinetic assays possible due to significant phenolate ion presence (and UV absorbance) at pH 5-6
- Simple format. Well suited to automation
- Standard included
(Aspergillus niger)
(Aspergillus niger)
碳水化合物结合模块 4B Carbohydrate Binding Module 4B ACZ03521 Carbohydrate Binding Module 4B 货号:ACZ03521 品牌:
Jinpan 碳水化合物结合模块 4B Carbohydrate Binding Module 4B ACZ03521
英文名:Carbohydrate Binding Module 4B
货号:ACZ03521
规格:1 mg
品牌: Jinpan
报价:¥2739.00
商品描述
P-MAL-PEG-N3 40k 丙酰胺马来酰亚胺聚乙二醇叠氮基 分子量MW 40000
上海金畔生物科技有限公司提供P-MAL-PEG-N3 40k 丙酰胺马来酰亚胺聚乙二醇叠氮基 分子量MW 40000
品牌:
货号:JPB-09100
中文名:丙酰胺马来酰亚胺聚乙二醇叠氮基 分子量MW 40000
英文名:P-MAL-PEG-N3 40k
端基取代率:》95%
纯度:》95%
分子量:40k/40000
分散度:《1.05
包装:1克/5克/10克
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订货热线:15221999938
qq号:2743691513
金畔博客:www.jinpanbio.cn
Email:sales@jinpanbio.com
15030 | Cyanine7 azide, 100 uL, 10 mM/DMSO – Lumiprobe荧光染料
上海金畔生物科技有限公司代理lumiprobe荧光染料全线产品,欢迎访问官网了解更多信息
15030 | Cyanine7 azide, 100 uL, 10 mM/DMSO
¥1,430.00
- 其他信息
其他信息
保存温度 |
-20°C |
---|---|
产地 |
美国 |
产品详情链接 |
https://www.lumiprobe.com/p/cy7-azide |
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