The CELLG5 assay reagent for the measurement of
endo-cellulase (endo-1,4-β-glucanase) contains two components;
1) 4,6-O-(3-Ketobutylidene)-4-nitrophenyl-β-D-cellopentaoside (BPNPG5) and 2) thermostable β-glucosidase. The ketone blocking group prevents any hydrolytic action by the
β-glucosidase on BPNPG5. Incubation with an endo-cellulase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary β-glucosidase. The rate of formation of 4-nitrophenol is therefore directly related to the hydrolysis of BPNPG5 by the endo-cellulase. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH 9.0).

Kit size:
K-CELLG5-4V  120 / 240 assays (manual) / 480 (auto-analyser)
 or
K-CELLG5-2V 60 / 120 assays (manual) / 240 (auto-analyser)
Method:  Spectrophotometric at 400 nm
Total assay time:  10 min
Detection limit: 3.5 x 10-4 U/mL
Application examples: 
Fermentation broths, industrial enzyme preparations, biofuels research
Method recognition: Novel method

Advantages

• Very cost effective
  
• All reagents stable for > 4 years
  
• Completely specific for cellulase (endo-1,4-glucanase)
  
• Generally applicable and highly sensitive
  
• Simple format. Well suited to automation
  
• Standard included

Megazyme 纤维素酶酶法测定K-CellG5操作视频