乳果糖快速检测方法—Megazyme乳果糖快速检测试剂盒

Megazyme乳果糖检测试剂盒采用酶法分析原理，可定量检测鲜奶、UHT奶、浓缩乳和奶粉等各种乳品中的乳果糖。

与农业部新标准采用相同的检测原理和方法

该试剂盒采用ISO方法11285：2004，经过改进后，更加快速和灵敏

灵敏度是传统己糖激酶法的两倍

成本低

试剂配制后可稳定保存2年

采用试剂盒形式，包含检测必需的所有酶

提供计算软件，使数据处理更方便

包含标准品

The Lactulose Assay Kit is suitable for the specific, rapid and sensitive measurement and analysis of lactulose in milk and milk-based samples. Reagents included in this kit may also be prepared for use in the procedure described by ISO Method 11285:2004.

UV-method for the determination of Lactulose in milk and
foodstuffs containing dairy products

Principle:
(β-galactosidase)
(1) Lactulose + H₂O → D-galactose + D-fructose

(glucose oxidase + catalase + H₂O₂)
(2) D-Glucose + H₂O + O₂ → D-gluconic acid + H₂O₂

(hexokinase)
(3) D-Fructose + ATP → F-6-P + ADP

(phosphoglucose isomerase)
(4) F-6-P → G-6-P

(glucose-6-phosphate dehydrogenase)
(5) G-6-P + NADP⁺ → gluconate-6-phosphate + NADPH + H⁺

(gluconate-6-phosphate dehydrogenase)
(6) Gluconate-6-phosphate + NADP⁺ → ribulose-5-phosphate + NADPH
+ CO₂ + H⁺

Kit size: 50 assays
Method: Spectrophotometric at 340 nm
Reaction time: ~ 120 min
Detection limit: 4.8 mg/L
Application examples:
Milk, dairy products and foods containing milk
Method recognition: Novel method

Advantages

• Twice the sensitivity of traditional hexokinase based lactulose methods
• Very cost effective
• All reagents stable for > 2 years after preparation
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing
• Standard included

相关产品：

K-GLUM    葡甘露聚糖检测试剂盒    Glucomannan Assay Kit
K-GLUT    L-谷氨酸[谷氨酸盐/谷氨酸酯/味精/谷氨酸单钠]检测试剂盒    L-Glutamic Acid Assay Kit
K-HDBA    D-3羟丁酸检测试剂盒    D-3-Hydroxybutyric Acid Assay Kit
K-INOSL    肌醇检测试剂盒    myo-Inositol Assay Kit
K-INTDF    综合总膳食纤维检测试剂盒    Integrated Total Dietary Fiber Assay Kit
K-ISOC    D-异柠檬酸检测试剂盒    D-Isocitric Acid Assay Kit
K-LACGAR    乳糖/D-半乳糖[快速]检测试剂盒    Lactose/Galactose Assay Kit (Rapid)
K-LACSU    乳糖/蔗糖/D-葡萄糖检测试剂盒    Lactose/Sucrose/D-Glucose Assay Kit
K-LACTUL    乳果糖检测试剂盒    Lactulose Assay Kit
K-LARGE    左旋精氨酸/尿/氨[快速]检测试剂盒    L-Arginine/Urea/Ammonia Assay Kit

The Glycerol GK test kit is a simple, reliable and accurate method for the measurement and analysis of glycerol in beverages, foodstuffs and other material. Based on use of ADP-glucokinase and increase in absorbance on conversion of NAD+ to NADH.
Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.
Suitable for manual, auto-analyser and microplate formats.

UV-method for the determination of Glycerol in foodstuffs,
beverages and other materials

Principle:
(glycerokinase)
(1) Glycerol + ATP → L-glycerol-3-phosphate + ADP

(ADP-GK)
(2) ADP + D-glucose → G-6-P + AMP

(glucose-6-phosphate dehydrogenase)
(3) G-6-P + NAD⁺ → gluconate-6-phosphate + NADH + H⁺

Kit size: 70 assays (manual) / 700 (microplate)
/ 600 (auto-analyser)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 7 min
Detection limit: 0.37 mg/L
Application examples:
Wine (and grape juice), beer, spirits, vinegar, marzipan, fruit juices,
soft drinks, toothpaste, honey, tobacco, paper (and cardboard),
cosmetics, pharmaceuticals, soap and other materials (e.g. biological
cultures, samples, etc.)
Method recognition: Novel method

Advantages

• Novel tablet format for increased stability
• Very competitive price (cost per test)
• All reagents stable for > 2 years as supplied
• Very rapid reaction
• Positive reaction (assay proceeds with an increase in absorbance)
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing
• Standard included
• Suitable for manual, microplate and auto-analyser formats

For the specific assay of L-ascorbic acid in beverages, meat, flour, dairy and vegetable products. Content:40 assays per kit

Colourimetric method for the determination of L-Ascorbic Acid 
in foodstuffs, feed, wine and other materials

Principle:
                        (5-methylphenazinium methosulphate)
(1) L-Ascorbic acid + R-H₂ + MTT → dehydroascorbate +                                                                                         MTT-formazan + H⁺
                            (ascorbic acid oxidase)
(2) L-Ascorbic acid + ½O₂ → dehydroascorbate + H₂O

Kit size:                             40 assays (manual) / 400 (microplate)
/ 400 (auto-analyser)
Method:                             Spectrophotometric at 578 nm
Reaction time:                   ~ 8 min
Detection limit:                  0.175 mg/L
Application examples:
Wine, beer, fruit juices, soft drinks, jam, milk, dairy products
(e.g. cheese), dietetic foods, baby foods, processed meat, baking
additives, fruit and vegetables (e.g. tomato and potato),
pharmaceuticals, feed and other materials (e.g. biological cultures,
samples, etc.)
Method recognition:
Methods based on this principle have been accepted by MEBAK

Advantages

• Very competitive price (cost per test)
• All reagents stable for > 6 months after preparation
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing
• Standard included
• Suitable for manual, microplate and auto-analyser formats