氨[快速]检测试剂盒 Ammonia (Rapid)

氨[快速]检测试剂盒 Ammonia (Rapid)
产品品名:氨快速检测试剂盒
英文品名:Ammonia assay Kit (Rapid)

货号:K-AMIAR
规格型号:96 assays (manual) (手动)/960次(微孔板)/960次(自动分析仪)
氨对地球上的生物相当重要,它是所有食物和肥料的重要成分。氨也是所有药物直接或间接的组成。氨有很广泛的用途,同时它还具有腐蚀性等危险性质。
反应时间:3min
检测限:0.07mg/L
适用样品:葡萄酒、水果汁、软饮料、乳制品(如酸奶)、保健食品、酱、蛋以及蛋产品、奶酪、肉、加工过的肉,海鲜、焙烤食品(和发酵粉)、焙烤食品(和发酵粉)、化肥、纸、医药品、烟、化妆品、水、凯氏定氮、纸(硬纸板)和其他原料(如生物培养基,样品等)
优点:
  •   由于适用不受抑制的谷氢酸脱氢酶,所以反应速度很快
  •   适用于手工和白动分析仪检测
  •   提供的酶为稳定的悬浮液
  •   成本低(每次检测成本
  •   所有试剂配制后的稳定性〉2年
  •   片剂形式增加了稳定性
  •   包含标准品
  •   该实验方法已通过MEBAK和德国的认证
商品说明:
For the rapid assay of ammonia in all samples, including grape juice and wine. Content:96 assays per kit
UV-method for the determination of Ammonia in foodstuffs,
beverages and other materials
Principle:
(microbial glutamate dehydrogenase)
(1) 2-Oxoglutarate + NADPH + NH4+ → L-glutamic acid + NADP+
+ H2O


Kit size:    96 assays (manual) / 960 (microplate)
/ 960 (auto-analyser)
Method:  pectrophotometric at 340 nm
Reaction time: ~ 3 min
Detection limit:  0.07 mg/L
Application examples:
Grape juice, wine, fruit juices, soft drinks, dairy products (e.g. milk),
dietetic food, soy sauce, eggs and egg products, cheese, meat,
processed meat, seafood, bakery products (and baking agents),
fertilisers, pharmaceuticals, tobacco, cosmetics, water, Kjeldahl
analysis, paper (and cardboard), water and other materials
(e.g. biological cultures, samples, etc.)
Method recognition:    
Methods based on this principle have been accepted by MEBAK
Advantages

  • Very rapid reaction due to use of uninhibited glutamate dehydrogenase
  • Enzyme supplied as stabilised suspension
  • Very competitive price (cost per test)
  • All reagents stable for > 2 years as supplied
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Extended cofactors stability
  • Suitable for manual, microplate and auto-analyser formats
    上海金畔生物科技有限公司(www.jinpanbio.com)提供生命科学研究领域系列产品,包括生化试剂、诊断试剂、实验耗材和仪器色谱标准品。Sigma生化试剂、Abcam、CST、Santa Cruz品牌的单克隆抗体、多克隆抗体以及BD Pharmingen的流式抗体,Invitrogen品牌试剂、R&D和Biovison品牌的ELisa试剂盒、Laysan bio、Avanti、NANOCS品牌的修饰性PEG(修饰性聚乙二醇)、Lumiprobe cy系列活性荧光染料。免疫诊断试剂包括Roche、TOYOBO、NEB品牌的酶、MP bio品牌的生化试剂和Amresco分子生物学试剂。标准品包括Sigma试剂、WAKO食品、农残、环境、水质等分析检测标准品、美国APSC品牌聚合物分子质量测量高分子量标准品、德国Dr和TRC以及药典USP、EP、中检所品牌标准品、Reagecon品牌的离子和各种金属标准品;耗材和仪器包括NUSSUI、BD difco、OXOID品牌的培养基、whatman、Millipore品牌的各种滤膜、滤器和柱子填料等、Reseach diets品牌的动物饲料、Labnet、康宁Corning、Axygen、Falcon 、Eppendorf品牌的离心机离心管、移液枪及枪头等实验室常用仪器耗材。
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服务热线:18301939375   QQ;3259632176
Email:  info@jinpanbio.com
官网:http://www.jinpanbio.com
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内切纤维素酶检测试剂盒

内切纤维素酶检测试剂盒

英文名:Cellulase Assay Kit (CELLG3 Method)

货号:K-CELLG3

规格:180 / 360 assays per kit / 720 (auto-analyser)

市场价: 5537

 纤维素酶是一种重要的酶产品,是一种复合酶,主要由外切β-葡聚糖酶、内切β-葡聚糖酶和β-葡萄糖苷酶等组成,还有很高活力的木聚糖酶。

提供内切纤维素酶检测试剂盒,色度法(400 nm)测定酶制品和发酵产品中的纤维素酶(内切-1,4-β-葡聚糖酶)。

内切纤维素酶检测试剂盒(CELLG3方法) K-CELLG3

使用高纯度β-葡萄糖苷酶和苯亚甲基阻断,2 – 氯-4 – 硝基苯基-β-Dcellotrioside(BClPNPβ-G3)。 β-葡萄糖苷酶的能力确保测定的可靠的最大的灵敏度。BClPNPβ-G3的水解为苯亚甲基,通过纤维素酶阻断纤维二糖和2-氯-4 -硝基苯基-β-D-葡萄糖, 2-Cl-4-硝基苯基-β-D-葡萄糖通过β-葡萄糖苷酶立即裂解为D-葡萄糖和游离的2 – 氯-4 – 硝基苯酚(ClPNP)。

反应时间:16min

适用样品:酶制品和发酵产品

优点:

价格低廉(每次检测成本)

特异性高

方法简单

包含标准品

 

The CELLG3 assay reagent for the measurement of endo-cellulase (endo-1,4-β-glucanase) contains two components;

1) 4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-D-cellotrioside (BCNPG3) and 2) thermostable β-glucosidase. The benzylidene blocking group prevents any hydrolytic action by the β-glucosidase on BCNPG3.  Incubation with an endo-cellulase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary β-glucosidase.  The rate of formation of 2-chloro-4-nitrophenol is therefore directly related to the hydrolysis of BCNPG3 by the endo-cellulase.  The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH 9.0).
Please note that a new assay kit (K-CELLG5) is now available for the measurement of endo-cellulase.  The CELLG5 reagent contains a cellopentaose core and exhibits vastly improved sensitivity for some cellulases.  In addition, the exchange of the benzylidene blocking group in CELLG3 for 3-keto-butylidene in CELLG5 improves the substrate’s water solubility significantly, allowing for a reduction in the concentration of DMSO required in the assay.  As DMSO is known to inhibit certain cellulases, this is another benefit in using CELLG5.  Megazyme now recommends the use of K-CELLG5 for all assays for the measurement of endo-cellulase.

Colourimetric method for the determination of 
endo
-1,4-β-glucanase (cellulase) in enzyme preparations and fermentationproducts

Principle:
(endo-1,4-β-glucanase)
(1) 3-Ketobutylidene-G5-β-PNP + H2O → Blocked-GX + G(5-X)-β-PNP

(thermostable β-glucosidase)
(2) G(5-X)-β-PNP + H2O → D-glucose + PNP

(alkaline solution)
(3) PNP → phenolate ion (yellow colour)
Note: PNP = 4-nitrophenol

Kit size:
K-CELLG5-4V 120 / 240 assays (manual) / 480 (auto-analyser)
or
K-CELLG5-2V 60 / 120 assays (manual) / 240 (auto-analyser)

Method:                         Spectrophotometric at 400 nm
Total assay time:           10 min
Detection limit:                3.5 x 10-4 U/mL
Application examples:
Fermentation broths, industrial enzyme preparations, biofuels research
Method recognition:     Novel method

 

一级氨基氮[NOPA]检测试剂盒

一级氨基氮[NOPA]检测试剂盒 Primary Amino Nitrogen Assay Kit (PANOPA)

名称:一级氨基氮[NOPA]检测试剂盒

英文名:Primary Amino Nitrogen(NOPA) Assay Kit

规格:100次

产地:爱尔兰

Primary Amino Nitrogen (PANOPA)

For the assay of primary amino nitrogen in grape juice/must and wine.

Catalogue Number: K-PANOPA
Content: 100 assays per kit

商品说明:

The Primary Amino Nitrogen (PANOPA) Assay Kit is suitable for the measurement and analysis of primary amino nitrogen in grape juice/must and wine.
Suitable for manual, auto-analyser and microplate formats.

UV-method for the determination of Primary Amino Nitrogen in
grape juice, must, wine and other materials

Principle:
(room temperature)
(1) Amino nitrogen + N-acetyl-L-cysteine + o-phthaldialdehyde →
isoindole derivative

Kit size: 100 assays (manual) / 1000 (microplate)
/ 1100 (autoanalyser)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 15 min
Detection limit: 2.59 mg N/L
Application examples:
Grape juice, must, wine and other materials
Method recognition: Novel method

Advantages

  • Simple format (absorbances read at 340 nm)
  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Suitable for manual, microplate and auto-analyser formats

Beta葡聚糖酶[麦芽和微生物]检测试剂盒

Beta葡聚糖酶[麦芽和微生物]检测试剂盒  β-Glucanase Assay Kit (Malt and Microbial)

1) 英文:Beta-Glucanase (Malt & Microbial) Assay Kit;

2) 中文:Beta葡聚糖酶[麦芽和微生物]检测试剂盒;

3) 规格: 100; 4) 货号:K-MBGL

商品说明:

β-Glucanase (Malt and Microbial) Assay Kit is suitable for the measurement and analysis of malt and bacterial β-glucanase and endo-1,4-β-glucanase.

Colourimetric method for the determination of β-Glucanase
in malt, foodstuffs and fermentation products

Principle:
(β-glucanase)
(1) Azo-Barley β-glucan (polymer) → Azo-barley β-glucan
(fragments)

(2) Add alcohol; centrifuge to remove polymeric Azo-barley
β-glucan

(3) Measure the absorbance of the supernatant solution

Kit size: 100 assays
Method: Spectrophotometric at 590 nm
Reaction time: ~ 30 min
Detection limit: 100 U/kg of malt
Application examples:
Malt extracts, wort, beer and other materials
Method recognition:
RACI (Standard Method)

Advantages

  • Very cost effective
  • All reagents stable for > 2 years during use
  • Only kit available
  • Very specific
  • Simple format
  • Standard included

D-乳酸检测试剂盒,D-Lactic Acid Assay Kit

D-乳酸检测试剂盒,D-Lactic Acid Assay Kit

名称:D-乳酸检测试剂盒
英文名:D-Lactic Acid Assay Kit
规格:50次
产地:爱尔兰
外观 :五玻璃瓶。
比重 :不适用
在水中的溶解度: 易溶
pH值: 7.0
气味: 无
粉末和液体形式
稳定性: 稳定 5年或以上(冷冻)

成分:
名称 比例
甘氨酰甘氨酸缓冲液 1瓶(玻璃)
烟酰胺腺嘌呤二核苷酸 1瓶(玻璃)
D-谷氨酸丙酮酸转氨酶 1瓶(玻璃)
D-乳酸脱氢酶酶 1瓶(玻璃)
D-乳酸的标准品 1瓶(玻璃)

许多食品和饮料中都发现有D-乳酸.  由乳酸菌产生, D-乳酸存在于牛奶的发酵产品中比如酸奶,腌菜,腌肉和腌鱼中。牛奶、肉、和果汁的质量可以通过测定D-乳酸的含量来确定。在葡萄酒生产中,D-乳酸的产生标志着酒被乳酸菌***。在化学工业中,D-乳酸和L-乳酸都是生产过程中聚合物的原料,比如聚交酯和一些生物学上可降解的聚合物。同时也应用在化妆品和医药品中。

原理:

D-乳酸的定量分析需要两步酶化反应。  第一个反应由D-乳酸脱氢酶(D-LDH)催化,D-乳酸在烟酰腺嘌呤二核苷酸(NAD+)的催化下,被氧化成丙***酸

然而, 因为反应(1)的移动和D-乳酸和NAD+有着密切的关系,下一步反应需要消耗掉产生的丙***酸 在大量D-谷氨酸的存在下,通过在D-谷氨酸-丙***酸转氨酶(D-GPT)的作用下把丙***酸转化为D-丙氨酸和2-***戊二酸。(2).

以上两个反应中产生的 NADH 的量可以用来计量D-乳酸的量。  NADH的量通过340nm处增加的吸光率来测量。

特异性,敏感度, 线性和准确性:

此方法特异性检测D-乳酸.

此方法特异性检测D-乳酸和L-乳酸。在D-乳酸锂(MW=96.0)的检测中,大约可以预期结果为96%(W/W),L-乳酸锂可以得到98%(W/W)。

检测的灵敏度为0.005 个吸光度单位。相当于样品最大体积为1.50 mL 时,样品浓度为0.107 mg/L(或者 1.60 mg/L ,样品体积0.1 mL) 。检测限为 0.214 mg/L,这是在0.010的吸光度差异,样品最大体积为1.50 mL为得到的 。

D-乳酸检测的线性变化为0.5 到 30 μg 。在重复测定中,同一份样品溶液,可能产生0.005 到 0.010 个吸光光度值的差异。这就相当于样品1.50 mL,样品溶液中D-乳酸的浓度为0.107 到 0.214 mg/L 。如果样品在制备阶段被稀释过,结果要乘以稀释倍数F。如果样品制备阶段,称量过样品,比如10 g/L,可能会产生0.02 到 0.05 g/100 g 的差异。

干扰:

如果D-乳酸的转换在实验指定的时间内(大约5分钟)彻底完成,通常认为是没有发生任何干扰。不过,这个也可以进一步验证,通过在实验的完成时,向比色皿中加入D-乳酸混合物(大约每0.1mL15ug),吸光光度值有会明显增加。

样品中的干扰物质的分析可以通过加入一个内参来确定。需要进行一个标准品定量回收实验,通过这个回收实验(也就是在最初的提取步骤中向样品中加入D-乳酸)可以算出样品在处理和提取过程中的损失量。

D-Lactic Acid (D-Lactate) (Rapid)
For the rapid and specific assay of D-lactic acid in wine, beer, juice, milk, cheese, vinegar and meat products.

Catalogue Number: K-DATE
Content: 50 assays per kit

Appearance Five glass vials.
Specific Gravity not applicable
Solubility in Water Readily soluble.
pH Value 7.0
Odour none
Form powders and liquids
Stability stable in a freezer for five or more years
Ingredients Name CAS Proportion
Glycylglycine buffer 1 vial (glass)
Nicotinamide adenine dinucleotide 1 vial (glass)
D-Glutamate pyruvate transaminase enzyme 1 vial (glass)
D-Lactate Dehydrogenase enzyme 1 vial (glass)
D-Lactic acid standard solution 1 vial (glass)

K-DATE  D-乳酸快速检测试剂盒
乙醛检测试剂盒K-ACHYD13
乙酸 (ACS;分析仪格式)检测试剂盒K-ACETAF13
乙酸(ACS;手工格式)检测试剂盒K-ACET14
乙酸(AK;分析仪格式)检测试剂盒K-ACETAK14
乙酸(RM;快速手工格式)检测试剂盒K-ACETRM15
α-淀粉酶(“Ceralpha”方法)检测试剂盒K-CERA15
氨检测试剂盒K-AMIA16
氨(快速)检测试剂盒K-AMIAR16
直链淀粉 /支链淀粉检测试剂盒K-AMYL17
阿拉伯糖检测试剂盒K-ARAB17
L-阿拉伯糖/ D-半乳糖(快速)检测试剂盒K-ARGA18
阿拉伯木聚糖检测试剂盒K-ARAXYL18
L-精氨酸 /尿素 /氨(快速)检测试剂盒K-LARGE19
L-抗坏血酸检测试剂盒K-ASCO19
L-天冬酰胺酸/ L-谷氨酸酰胺/氨(快速)检测试剂盒K-ASNAM20
阿斯巴甜检测试剂盒K-ASPTM20
可用碳水化合物 /膳食纤维检测试剂盒K-ACHDF21
?-淀粉酶(“Betamyl”方法)检测试剂盒K-BETA21
β-(Mixedlinkage)检测试剂盒K-BGLU22
β-检测试剂盒K-YBGL22
β-葡聚糖酶(麦芽和微生物)检测试剂盒K-MBGL23
K-DATE  D-乳酸快速检测试剂盒
胆固醇检测试剂盒K-CHOL23
柠檬酸检测试剂盒K-CITR24
乙醇检测试剂盒K-ETOH24
蚁酸检测试剂盒K-FORM25
果糖(己糖激酶格式)检测试剂盒K-FRUCHK25
果糖(PAHBAH格式)检测试剂盒K-FRUC26
D-果糖 / D-葡萄糖检测试剂盒K-FRUGL26
D-果糖 / D-葡萄糖 (MegaQuant格式)检测试剂盒K-FRGLMQ27
半乳甘露聚糖检测试剂盒K-GALM27
葡甘露聚糖检测试剂盒K-GLUM28
D-葡萄糖酸 / D-葡萄糖酸内酯检测试剂盒K-GATE28
D-葡糖胺检测试剂盒K-GAME29
D-葡萄糖 (GOPOD格式)检测试剂盒K-GLUC29
D-葡萄糖(己糖激酶格式)检测试剂盒K-GLUHKR/L30
葡糖氧化酶检测试剂盒K-GLOX30
L-谷氨酰胺 /氨(快速)检测试剂盒K-GLNAM31

L-谷氨酸 (MSG)检测试剂盒K-GLUT31
甘油检测试剂盒K-GCROL32
D-3-羟丁酸检测试剂盒K-HDBA32
D-异柠檬酸检测试剂盒K-ISOC33
D-乳酸检测试剂盒K-DATE33
D-/L-乳酸检测试剂盒K-DLATE34
K-DATE  D-乳酸快速检测试剂盒
L-乳酸检测试剂盒K-LATE34
乳糖 / D-半乳糖(快速)检测试剂盒K-LACGAR35
乳糖/蔗糖 / D-半乳糖检测试剂盒K-LACSU35
乳果糖检测试剂盒K-LACTUL36
D-苹果酸检测试剂盒K-DMAL36
L-苹果酸检测试剂盒K-LMALR/L37
L-苹果酸 (Analyser格式)检测试剂盒K-LMALAF37
L-苹果酸(MegaQuant格式)检测试剂盒K-LMALMQ38
麦芽糖/蔗糖/ D-葡萄糖检测试剂盒K-MASUG38
D-甘露醇 / L-阿拉伯糖醇检测试剂盒K-MANOL39
D-甘露糖 / D-果糖 / D-葡萄糖检测试剂盒K-MANGL39
果胶鉴定检测试剂盒K-PECID40
植酸检测试剂盒K-PHYT40
Primary氨基氮 (NOPA)检测试剂盒K-PANOPA41
丙酮酸检测试剂盒K-PYRUV41
棉籽糖/ D-半乳糖检测试剂盒K-RAFGA42
棉籽糖 /蔗糖 / D-葡萄糖检测试剂盒K-RAFGL42
抗性淀粉检测试剂盒K-RSTAR43
D-山梨醇 /木糖醇检测试剂盒K-SORB43
破损淀粉检测试剂盒K-SDAM44
琥珀酸检测试剂盒K-SUCC44
蔗糖 / D-果糖 / D-葡萄糖检测试剂盒K-SUFRG45
K-DATE  D-乳酸快速检测试剂盒
蔗糖 / D-葡萄糖 (GOPOD格式)检测试剂盒K-SUCGL45
总膳食纤维检测试剂盒K-TDFR46
总淀粉 (GOPOD格式)检测试剂盒K-TSTA46
总淀粉(己糖激酶格式)检测试剂盒K-TSTAHK47
海藻糖检测试剂盒K-TREH47
尿素/氨(快速)检测试剂盒K-URAMR48
木聚糖酶 (Azo-Wax格式)检测试剂盒K-AZOWAX48
木聚糖酶(木聚糖酶 AX格式)检测试剂盒K-XYLS49
D-木糖检测试剂盒K-XYLOSE49