同仁化学Hampton蛋白结晶试剂盒Mounted CryoLoop™ – 20 micron/HR4-963/HR4-959/HR4-957| 日本DOJINDO

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Products > Cryocrystallography > CryoLoops > Mounted CryoLoop™ – 20 micron

Mounted CryoLoop™ – 20 micron

应用

  • Cryocrystallography
  • 低温晶体学

特征

  • Synthetic CryoLoop on stainless steel MicroTube
  • MicroTube specially engineered with EasySnap notches
  • Complete range of loop diameters from 0.025 – 1.0 mm
  • Compatible with all CrystalCap Systems
  • 不锈钢 MicroTube 上的合成 CryoLoop
    MicroTube 特别设计,带有 EasySnap 凹槽
    从 0.025 – 1.0 mm 的完整范围的环直径
    与所有 CrystalCap 系统兼容

描述

Note: If you are using the CrystalCap, CrystalCap ALS, CrystalCap ALS HT, or CrystalCap SPINE HT, save time and money by using the ready to mount 18 mm Mounted CryoLoop.

Mounted CryoLoops with 20 micron diameter nylon. These nylon loops are bonded to hollow, stainless steel MicroTubes™ that are used to mount, freeze, and secure the crystal during cryocrystallographic procedures and X-ray data collection. The MicroTube is 24 mm in length and is specially engineered with EasySnap™ notches at the 10, 12, 14, 18, and 21 mm measures. To obtain the desired length of MicroTube, simply snap the MicroTube at the desired length and secure to the CrystalCap using epoxy or similar adhesive. These nylon loops show minimal diffraction, are thin for fast freezing, strong, and aerodynamic..

Most data collection geometries prefer or require a 22 mm length between the base of the cap and the beam. For a 22 mm overall length using the CrystalCap, CrystalCap ALS, CrystalCap ALS HT and CrystalCap SPINE HT, snap the MicroTube at the second notch from the bottom, creating an 18 mm MicroTube lenghth and secure to the CrystalCap using epoxy or similar adhesive. For a 22 mm over length using the CrystalCap Copper, CrystalCap Copper HT, CrystalCap ALS Copper HT snap the MicroTube at the fifth notch from the bottom (notch closest to the CryoLoop) and secure to the CrystalCap using epoxy or similar adhesive.

注意:如果您使用的是 CrystalCap、CrystalCap ALS、CrystalCap ALS HT 或 CrystalCap SPINE HT,请使用可安装的 18 毫米已安装 CryoLoop 来节省时间和金钱。

安装有 20 微米直径尼龙的 CryoLoops。这些尼龙环与中空不锈钢 MicroTubes™ 粘合,用于在低温晶体学程序和 X 射线数据收集过程中安装、冷冻和固定晶体。 MicroTube 的长度为 24 毫米,专门设计了 10、12、14、18 和 21 毫米尺寸的 EasySnap™ 凹槽。要获得所需长度的 MicroTube,只需将 MicroTube 卡入所需长度并使用环氧树脂或类似粘合剂固定到 CrystalCap。这些尼龙环显示出最小的衍射,很薄,可以快速冷冻,坚固且符合空气动力学。

大多数数据收集几何结构更喜欢或要求帽底和梁之间有 22 毫米的长度。对于使用 CrystalCap、CrystalCap ALS、CrystalCap ALS HT 和 CrystalCap SPINE HT 的 22 mm 总长度,将 MicroTube 从底部的第二个凹口处卡入,形成 18 mm 的 MicroTube 长度并使用环氧树脂或类似粘合剂固定到 CrystalCap。对于 22 mm 以上的长度,使用 CrystalCap Copper、CrystalCap Copper HT、CrystalCap ALS Copper HT 将 MicroTube 卡在从底部算起的第五个槽口(最靠近 CryoLoop 的槽口),并使用环氧树脂或类似粘合剂固定到 CrystalCap。

HR4-965 Mounted CryoLoop 0.7 – 1.0 mm

Hampton蛋白结晶试剂盒Mounted CryoLoop™ – 20 micron/HR4-963/HR4-959/HR4-957
Hampton蛋白结晶试剂盒Mounted CryoLoop™ – 20 micron/HR4-963/HR4-959/HR4-957
Hampton蛋白结晶试剂盒Mounted CryoLoop™ – 20 micron/HR4-963/HR4-959/HR4-957
Hampton蛋白结晶试剂盒Mounted CryoLoop™ – 20 micron/HR4-963/HR4-959/HR4-957
Hampton蛋白结晶试剂盒Mounted CryoLoop™ – 20 micron/HR4-963/HR4-959/HR4-957

CAT NO

HR4-313

NAME

Mounted CryoLoop – 20 micron

DESCRIPTION

0.025 – 0.05 mm – 25 pack

PRICE

$84.00

CAT NO

HR4-625

NAME

Mounted CryoLoop – 20 micron

DESCRIPTION

0.05 – 0.1 mm – 25 pack

PRICE

$84.00

CAT NO

HR4-955

NAME

Mounted CryoLoop – 20 micron

DESCRIPTION

0.1 – 0.2 mm – 25 pack

PRICE

$84.00CAT NO

HR4-957

NAME

Mounted CryoLoop – 20 micron

DESCRIPTION

0.2 – 0.3 mm – 25 pack

PRICE

$84.00

CAT NO

HR4-959

NAME

Mounted CryoLoop – 20 micron

DESCRIPTION

0.3 – 0.4 mm – 25 pack

PRICE

$84.00

CAT NO

HR4-961

NAME

Mounted CryoLoop – 20 micron

DESCRIPTION

0.4 – 0.5 mm – 25 pack

PRICE

$84.00

CAT NO

HR4-963

NAME

Mounted CryoLoop – 20 micron

DESCRIPTION

0.5 – 0.7 mm – 25 pack

PRICE

$84.00

CAT NO

HR4-965

NAME

Mounted CryoLoop – 20 micron

DESCRIPTION

0.7 – 1.0 mm – 25 pack

PRICE

$84.00

CAT NO

HR4-953

NAME

Mounted CryoLoop – 20 micron

DESCRIPTION

Sampler – 30 pack (5 of each diameter size 0.1 – 1.0 mm)

PRICE

$103.00

参考

1. FeeM, an N-acyl amino acid synthase from an uncultured soil microbe: Structure, mechanism, and acyl carrier protein binding. Ryan M. Van Wagoner and Jon Clardy. Structure, 2006, Volume 14, Number 9, 1425-1435.

1. FeeM,一种来自未培养土壤微生物的 N-酰基氨基酸合酶:结构、机制和酰基载体蛋白结合。 瑞安 M. 范瓦格纳和乔恩克拉迪。 结构,2006 年,第 14 卷,第 9 期,1425-1435。

Encapsula Immunosome-Cyanur (PEGylated) 货号:IMS-2005-5ML 品牌:Encapsula NanoSciences


Encapsula Immunosome-Cyanur (PEGylated)

货号:IMS-2005-5ML

规格:5ml

品牌: Encapsula NanoSciences

报价:13775.00

商品描述

In order to develop a rapid and straightforward coupling procedure at the PEG terminus, a method of direct coupling antibodies to the PEG terminus of liposomes was introduced by Bendas et al. [1]. In this methodology, antibodies are simply attached to the PEG terminus of liposomes, which had been end-group functionalized with cyanuric chloride, in mild basic conditions (pH 8.8) without prior antibody derivatizations. It has been shown that in order to obtain a stable attachment of proteins on liposome, the DSPE-PEG-cyanur was added into the liposomes to chemically conjugate with proteins to form a stable complex and minimize the denaturation of proteins. Proteins can be covalently coupled to the liposomes via amine-reactive cyanur-groups, either directly to the vesicle surface using cyanuric chloride-activated DSPE (cyanur-DSPE) or to the distal ends of PEG-spacers using activated cyanur-PEG-PE (ammonium salt). Cyanuric chloride at the PEG terminus functions to link peptides, antibodies and other amine-containing biomolecules or nanoparticles via a nucleophilic substitution reaction under basic conditions. Antibodies or other proteins can be conjugated without any previous derivatization.