禽类支原体疾病抗体检测试剂盒 货号:MGA-5P 品牌:


禽类支原体疾病抗体检测试剂盒

货号:MGA-5P

规格:640 test samples

品牌: Jinpan

报价:5400.00

商品描述

每个套件包含大约640个测试样品加控制,包含5个微量滴定板。
 

 

Mycoplasma gallisepticum is a cause of respiratory disease, egg-productions drops, and reduced feed conversion. Severe airsacculitis, coughing, rales and poor growth may be observed in broilers, with heavy condemnations at processing. MG is egg transmitted, and may be spread laterally by direct or indirect contact. Mycoplasma infections of poultry may be readily confused with other respiratory diseases and may be obvious only when present in conjunction with complicating respiratory infections such as Newcastle disease or infectious bronchitis1. Antibodies in serum can be detected by the Enzyme Linked Immunosorbant Assay (ELISA). However, other serologic testing methods such as serum plate agglutination and culturing techniques are recommended for confirmation of infection1. The levels of MG antibodies in serum, as well as the immune status of entire flocks can be monitored using ELISA.
鸡毒支原体是呼吸道疾病的病因,鸡蛋生产下降,并降低饲料转化。严重的气囊炎,咳嗽,罗音和穷人的经济增长可能会观察到在肉仔鸡,在处理重的谴责。镁是蛋传播,和可横向通过直接或间接接触传播。家禽支原体感染可以很容易与其他呼吸道疾病相混淆,可明显只有当存在与合并呼吸道感染等疾病或传染bronchitis1纽卡斯尔。血清中的抗体可通过酶联免疫吸附试验(ELISA)检测。然而,其他的血清学检测方法如血清平板凝集和培养技术推荐确认感染。血清镁的抗体水平,以及整个鸡群免疫状态可监测使用ELISA。
Inactivated antigen is bound to a microwell. Diluted sera is added to the microwell and incubated. Any anti-MG antibodies present in the serum will bind to the MG on the microwell forming an MG antigen-antibody complex. After washing away any unbound material, anti-Chicken IgG Alkaline Phosphatase conjugate is added to the microwell. The conjugate will bind to the MG antigen-antibody complex. After incubation any unbound conjugate is removed by washing. Enzyme substrate is then added to the microwell. The substrate reacts with any Alkaline Phosphatase present and forms a yellow product. In the final step the reaction is stopped, fixing the color. The intensity of the color is measured photometrically at 405 – 410 nm.
灭活抗原绑定到微孔。稀释的血清加入微孔培养。任何抗MG血清中的抗体将结合在微孔形成抗原抗体复合物的镁镁。洗去任何未结合的物质后,抗鸡IgG碱性磷酸酶缀合物加入到微孔。共轭将绑定到Mg抗原抗体复合物。孵育后洗涤除去任何未结合的。酶的底物,然后添加到微孔。基板有碱性磷酸酶存在反应并形成黄色产物。在最后一步反应停止,固定的颜色。颜色的强度是在405 – 410 nm光度测量。

普兰德/BRAND 混合量筒,BLAUBRAND?, A级,10:0.2 ml,Boro 3.3, NS10/19,含PP量筒塞 Mixing cylinder, BLAUBRAND?, cl.A, 10:0,2ml, Boro 3.3, NS10/19 PP-stopper 货号:BRAND32408 品牌:BRAND


普兰德/BRAND 混合量筒,BLAUBRAND?, A级,10:0.2 ml,Boro 3.3, NS10/19,含PP量筒塞

英文名:Mixing cylinder, BLAUBRAND?, cl.A, 10:0,2ml, Boro 3.3, NS10/19 PP-stopper

货号:BRAND32408

规格:2个

品牌: BRAND

报价:538.00

商品描述

普兰德/BRAND 混合量筒,BLAUBRAND?, A级,10:0.2 ml,Boro 3.3, NS10/19,含PP量筒塞
Mixing cylinder, BLAUBRAND?, cl.A, 10:0,2ml, Boro 3.3, NS10/19 PP-stopper

iFluor 647-刀豆蛋白A(ConA)缀合物 货号25595-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

iFluor 647-刀豆蛋白A(ConA)缀合物

iFluor 647-刀豆蛋白A(ConA)缀合物

iFluor 647-刀豆蛋白A(ConA)缀合物    货号25595-AAT Bioquest荧光染料 货号 25595 存储条件 在零下15度以下保存, 避免光照
规格 1 mg 价格 1200
Ex (nm) 656 Em (nm) 670
分子量 N/A 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号:25595

产品名称:iFluor 647-刀豆蛋白A(ConA)缀合物

规格:1mg

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

Ex(nm):656

Em(nm):670

量子产率(cm -1 M -1):250000

消光系数:0.25

 

适用仪器


荧光显微镜  
激发: Cy5滤波片
发射: Cy5滤波片
推荐孔板: 黑色透明

 

产品介绍

刀豆蛋白A(ConA)是一种凝集素,它与各种糖、糖蛋白和糖脂中的某些结构特异结合。Con A是一种著名的T细胞有丝分裂原,它能激活免疫系统,募集淋巴细胞并诱导细胞因子的产生。除了有丝分裂活性外,ConA还可以通过线粒体介导的细胞凋亡和自噬诱导细胞程序性死亡。ConA还被报道激活NFAT(活化T细胞的核因子),NFAT是一个转录因子家族,在免疫系统的发育和功能中起重要作用,包括T细胞受体(TCR)的参与。ConA广泛应用于生物学和生物化学中,用来表征细胞表面的糖蛋白和其他含糖物质。还可用于凝集素亲和层析纯化糖基化大分子,以及研究各种免疫细胞的免疫调节作用。ConA在B-聚糖分支结构的末端位置特异性地结合α-D-甘露糖基和α-D-葡萄糖基残基(仅在碳2上的醇中不同的两个己糖)。它有4个结合位点,对应于4个亚单位。刀豆蛋白A(conA)是细胞生物学中应用最广泛的凝集素之一。iFluor 647-刀豆蛋白A(ConA)缀合物选择性地结合到A-甘露吡喃糖基和A-葡萄糖吡喃糖基残基上,发出明亮的红色荧光。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的iFluor 647-刀豆蛋白A(ConA)缀合物。

点击查看光谱

 

参考文献

A Rabbit Model of Aqueous-Deficient Dry Eye Disease Induced by Concanavalin A Injection into the Lacrimal Glands: Application to Drug Efficacy Studies.
Authors: Honkanen, Robert A and Huang, Liqun and Rigas, Basil
Journal: Journal of visualized experiments : JoVE (2020)

Acupuncture stimulation attenuates TNF-α production via vagal modulation in the concanavalin A model of hepatitis.
Authors: Lim, Hee-Don and Kim, Ki-Joong and Jo, Byung Gon and Park, Ji-Yeun and Namgung, Uk
Journal: Acupuncture in medicine : journal of the British Medical Acupuncture Society (2020): 964528420907338

Antioxidant Capacity and Hepatoprotective Role of Chitosan-Stabilized Selenium Nanoparticles in Concanavalin A-Induced Liver Injury in Mice.
Authors: Bai, Kaikai and Hong, Bihong and He, Jianlin and Huang, Wenwen
Journal: Nutrients (2020)

Concanavalin A Enhanced Proliferation and Osteogenic Differentiation of Dental Pulp Stem Cells.
Authors: Suardita, Ketut and Arundina, Ira and Tedjosasongko, Udijanto and Yuliati, Anita and Peeters, Harry Huiz and Wijaksana, I Komang Evan and Surboyo, Meircurius Dwi Condro
Journal: European journal of dentistry (2020): 123-127

Concanavalin A Toxicity Towards Potato Psyllid and Apoptosis Induction in Midgut Cells.
Authors: Tang, Xiao-Tian and Ibanez, Freddy and Tamborindeguy, Cecilia
Journal: Insects (2020)

Concanavalin A-Rose Bengal bioconjugate for targeted Gram-negative antimicrobial photodynamic therapy.
Authors: Cantelli, Andrea and Piro, Francesca and Pecchini, Pietro and Di Giosia, Matteo and Danielli, Alberto and Calvaresi, Matteo
Journal: Journal of photochemistry and photobiology. B, Biology (2020): 111852

Deficiency of O-linked-glycosylation regulates activation of T cells and aggravates Concanavalin A-induced liver injury.
Authors: Hao, Xiaohua and Gao, Meixin and He, Lingling and Ye, Xiaohui and Yang, Junru and Zhang, Fuyang and Liu, Ran and Wei, Hongshan
Journal: Toxicology (2020): 152411

Demethyleneberberine attenuates concanavalin A-induced autoimmune hepatitis in mice through inhibition of NF-κB and MAPK signaling.
Authors: Zhang, Miao and Li, Qingxia and Zhou, Cuisong and Zhao, Yaxing and Li, Ruiyan and Zhang, Yubin
Journal: International immunopharmacology (2020): 106137

Developmental exposure to low doses of dichlorodiphenyltrichloroethane impairs proliferative response of thymic lymphocytes to Concanavalin A in rats.
Authors: Yaglova, Nataliya V and Tsomartova, Elina S and Obernikhin, Sergey S and Ivanova, Marina Y and Chereshneva, Elizaveta V and Muhamedova, Svetlana G and Lomanovskaya, Tatiana A and Yaglov, Valentin V
Journal: Heliyon (2020): e03608

ISFET and Dex-AgNPs based portable sensor for reusable and real-time determinations of concanavalin A and glucose on smartphone.
Authors: Zhao, Shuang and Shi, Cong and Hu, Hongyang and Li, Zhengping and Xiao, Gang and Yang, Qiaochun and Sun, Peng and Cheng, Linyang and Niu, Wencheng and Bi, Jinshun and Yue, Zhao
Journal: Biosensors & bioelectronics (2020): 111962

说明书
iFluor 647-刀豆蛋白A(ConA)缀合物.pdf