A modification of AOAC Method 996.11 AACC Method 76-13.01 RACI Standard Method for the measurement and analysis of total starch in cereal flours and food products. This kit contains an improved α-amylase that allows the amylase incubations to be performed at pH 5.0 (as well as pH 7.0). The method has been further modified by adjusting the D-glucose determination to a hexokinase/glucose-6-phosphate dehydrogenase/NADP+ based format.

UV-method for the determination of Total Starch in grains,
animal feeds, foodstuffs and other materials

Principle:
(α-amylase, 100°C + DMSO)
(1) Starch granules + H₂O → maltodextrins

(amyloglucosidase)
(2) Maltodextrins + H₂O → D-glucose

(hexokinase)
(3) D-Glucose + ATP → G-6-P + ADP

(glucose-6-phosphate dehydrogenase)
(4) G-6-P + NADP⁺ → gluconate-6-phosphate + NADPH + H⁺

Kit size:  100 assays
Method: Spectrophotometric at 340 nm
Total assay time:  ~ 90 min
Detection limit:  1-100% of sample weight
Application examples: 
Cereal flours, food products and other materials
Method recognition: 
AOAC (Method 996.11), AACC (Method 76-13.01), ICC (Standard
Method No. 168), and RACI (Standard Method)

Advantages

• Very competitive price (cost per test)
  
• All reagents stable for > 2 years after preparation
  
• Simple format
  
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  
• Standard included

Sample Pre-treatment Format:

Total Starch (AA/AMG) Assay Kit:

Substrate for the specific measurement of endo-1,4-ß-D-glucanase (cellulase).

葡聚糖酶检测底物

PDF Download

特点：

1) 体积较小，方便便携

2) 布质柔和的面料能合适地安置玻璃容器，减少了热击的可能性；

3) 适合于包括大容器在内的多种容器；

4) 可承受400℃的内部工作温度， 可提供精确的控制，范围为3 – 100% ；

种类：

1) O系列加热套

2) 微型加热套

3) 加热/隔热盖

CAS: 158962-91-5
Molecular Formula: C₂₀H₂₄O₁₁
Molecular Weight: 440.4
Purity: > 98%

High purity 4-Methylumbelliferyl-β-xylobioside for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

A fluorogenic substrate for assay of xylanase and xlyosidase activity. 

Data booklets for each pack size are located in the Technical Resources tab.

High purity dyed and crosslinked Cellazyme T tablets for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis. 

A unique substrate for the measurement of endo-cellulases active on highly branched and insoluble cellulosic substrates. Containing AZCL-Xyloglucan. Highly sensitive substrate.

PDF Download