Hampton MembFac • Crystal Screen Lite • MembFac HT
Primary sparse matrix crystallization screen for membrane proteins and samples with limited solubility 膜蛋白和样品溶解度有限样品
Membrane protein sparse matrix screens
Sparse matrix formula efficiently samples salts, polymers, organics and pH
pH range 4.6 – 8.5
Formulated for use with detergents
Tube or Deep Well block format
Crystal Screen Lite features a lower ionic strength than the original Crystal Screen
MembFac is a highly effective sparse matrix screen specifically designed as a preliminary screen for the crystallization of membrane proteins. MembFac is based upon the highly effective biased sparse matrix methodology. The MembFac screen matrix is optimized for the crystallization of membrane proteins.
MembFac contains 48 unique reagent formulations. With this set of 48 conditions, numerous chemicals and chemical combinations are utilized, allowing one to evaluate a large variety of potential crystallization conditions. MembFac covers a broad pH range between 4 and 9.
The basic rationale of MembFac is to perform a sparse matrix screen while changing the detergent dimension because detergents play an important factor in the crystallization of membrane proteins. A MembFac screen is to be completed for each detergent. Detergent screens are available separately.
The membrane protein of interest is first isolated in the detergent which gives the highest stability and activity. The protein concentration should be approximately 10 to 20 mg/ml and the detergent concentration should be only slightly above the CMC. The sample is then set against MembFac, using all 48 solutions plus the screening detergent(s) of choice. The screening detergent concentration should be approximately one to three times the CMC. Hence, for each detergent screened, one would utilize approximately 1 to 2 mg of sample.
The MembFac protocol was used to crystallize membrane proteins including SQR, fumerate: quinone oxireductase, LHII, and LHCII.
Crystal Screen Lite is a sparse matrix of trial crystallization reagent conditions based upon the Crystal Screen™.3 The primary screen variables are salt, pH, and precipitant (salts, polymers, volatile organics, and non-volatile organics).2Crystal Screen Lite differs from the original Crystal Screen kit in that the primary precipitant reagents are one-half the concentration of that used in the original Crystal Screen formulation. The secondary salts, ions, and buffers remain at the original Crystal Screen concentration. Reducing the primary concentration of the primary precipitant results in a screen which is “more gentle” on the sample and typically produces much less precipitate conditions than the original Crystal Screen.
MembFac contains 48 unique reagents, 10 ml each.
MembFac HT contains 1 ml each of all 48 reagents from MembFac and reagents 1-48 from Crystal Screen Lite in a single Deep Well block format.
Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.
|HR2-114||MembFac||10 ml, tube format|
|HR2-128||Crystal Screen Lite||10 ml, tube format|
|HR2-137||MembFac HT||1 ml, Deep Well block format|
Hampton AlumaSeal™ II Sealing Film and Applicator
Sealing film used to reseal HT format screen kits in polypropylene blocks and plates
|Film conforms to raised chimney wells|
|Easily pierceable with single or multichannel pipettors and robotic probes|
|Heat & cold resistant, recommended for temperatures from -80 °C to +120 °C|
|Certified DNase-, RNase-, and nucleic-acid-free|
|Less evaporation than clear films|
|Excellent barrier properties, virtually no reagent evaporation or drying|
A 38 µm soft non-permeable aluminum foil sealing film with strong medical-grade adhesive, AlumaSeal II sealing films eliminate the need for heat-sealing devices or mats during the resealing of reagents in polypropylene deep well blocks. Each sealing film measures 82.6 x 142.9 mm and offers sufficient sealing area for 96 deep well blocks. Length between the perforations with end tabs removed is 125.4 mm. Compared to other aluminum foils, AlumaSeal II has less tendency to roll back on itself when removing the backing paper and it conforms well to the plate during application.AlumaSeal II is a soft, pierceable adhesive film designed for the convenient and rapid sealing of polypropylene deep well blocks. A multiple split backing with two end tabs allows for easy, accurate positioning and secure sealing. The use of an adhesive sealing film minimizes evaporation and helps to prevent well-to-well cross contamination in reagent blocks. AlumaSeal II films are easily pierced by pipettte tips or robotic probes or piercing tools for direct reagent recovery without significant gumming by adhesive.
|HR8-069||AlumaSeal II Sealing Film||100 pack|
|HR4-413||Film Sealing Paddle||5 pack|
Hampton Research是 美国最权威的蛋白结晶试剂盒提供商，产品齐全，性价比高。
美国Hampton Research公司位于加利福尼亚州，是一家专门从事蛋白质晶体研究的生产商。该公司向全球科研人员提供全面的晶体研究试剂和相关实验耗材，同时提供晶体设计和合成方面的定制服务。Hampton Research公司以其先进的生物大分子结晶技术和广泛实用的产品线，不仅为全世界晶体研究人员的工作带来了便捷，而且也受益于科研人员的回馈不断发展自己，目前已成为晶体研究领域最受信赖的品牌之一。
Crystallization Screens 用于筛选和优化蛋白质/多肽/核酸结晶的一系列经验证的高精配方型溶液
Optimize Reagents 结晶级别盐类，聚合物，有机溶液或缓冲液
Crystallization Plates, Hardware & Accessories 结晶板（悬滴法和坐滴法，微量透析，微配液，自由界面扩散，凝胶以及无容器悬浮法）及其他配件
Cryocrystallography 低温晶体学耗材如 Crystalcap system,cryoloops
Goniometer Heads&Supplies 测角器头和配件
Protein Crystallization Standards 结晶级别标准蛋白
Custom Shop Crystallization Reagents 定制试剂盒和结晶溶液内的单一组分
StockOptions Kits 内含盐类，缓冲液和冷冻液的简便型试剂盒
Capillary Mounts & Supplies 毛细管,玻璃纤维, 蜡,黏合剂和密封剂
- StockOptions pH Buffer Kit HR2-241
Crystallization grade buffer stocks for crystal screening, optimization, & production
Crystal Screen Cryo HR2-122
Primary screen with cryo for proteins, soluble peptides, nucleic acids, & water soluble small molecules
- Natrix HR2-116
Primary biased sparse matrix crystallization screen for nucleic acids & protein/nucleic acid complexes
- MembFac HR2-114
Primary sparse matrix crystallization screen for membrane proteins and samples with limited solubility
- SaltRx HR2-136
Primary or secondary, salt and pH matrix crystallization screen for biological macromolecules
- Grid Screens HR2-248
Primary or secondary, salt, polymer, organic and pH grid crystallization screen for biological macromolecules
- PEG/Ion HR2-126
Primary or secondary, polymer, salt and pH matrix crystallization screen for biological macromolecules
- PCT™ Pre-Crystallization Test HR2-140
Determine the appropriate sample concentration for crystallization screening
- Index HR2-144
Primary, diverse reagent system crystallization screen for proteins, complexes, peptides, nucleic acids, & water soluble small molecules
- Crystal Screen HR2-110
Primary screen for proteins, soluble peptides, nucleic acids, & water soluble small molecules;Sparse matrix additive screen
- PEGRx HR2-082
Primary and secondary, polymer and pH based crystallization screen for biological macromolecules
- Additive Screen HR2-428
Manipulate sample-sample & sample-solvent interactions to improve crystals or alter sample solubility
- Detergent Screen HR2-408
Prevent and manipulate non-specific aggregation due to hydrophobic interactions
- CryoPro HR2-073
Water soluble cryoprotectant reagent set designed for the cryopreservation of biological macromolecular crystals
- StockOptions Salt HR2-245
Crystallization grade salt reagent stock solutions for screen formulation and optimization
- Siliconized Glass Cover Slides HR3-217
Hanging, sitting or sandwich drop crystallization
- Intelli-Plate 96 HR3-185
- CrystalCap HR4-733
- CryoTong™ – Long HR5-104
Crystal transfer under cryo temperature
- CrystalWand™ Magnetic HR4-729
CrystalCap Magnetic & CrystalCap Copper Magnetic handling tool
- Vial Clamp™ – Straight HR4-670
Vial support and manipulation
- CrystalCap™ Holder HR4-707
Organized storage of CrystalCaps
- CryoTong™ – Long HR5-104
|Sitting drop crystallization|
|One drop per reservoir|
|48 well plate|
|9 mm standard distance between wells|
|Drop volume: Up to 10 µl|
|Reservoir volume: 50 to 200 µl|
|Micro-numbering alongside drop volumes|
|Rigid plate structure|
|Wide partition walls between wells improve sealing|
|Developed in conjunction with the MRC Laboratory of Molecular Biology in Cambridge, United Kingdom|
|UV compatible (UVP)|
MRC Maxi 48-Well Crystallization Plate for Automated Optimization
The MRC Maxi optimization plate is a breakthrough for macromolecular crystallization presented in a 48 well format. Offering easy to automate crystallization optimization with large sitting-drops, the new MRC Maxi Crystallization plate is the perfect solution. Manufactured by Swissci AG, the plate offers an SBS format while providing 48 wells. MRC Maxi is intended for large drops and is compatible both with standard robotic systems as well as manual pipetting.
The plate was developed at the MRC Laboratory of Molecular Biology (Cambridge, UK) in collaboration with Jan Löwe and Fabrice Gorrec. It is a result of many years of experience in successful robotic high-throughput crystallization and complements the original MRC crystallization plate, which is intended for smaller drop volumes and higher throughput during screening.
Drop volumes of up to 10 µl are possible. The 9 mm standard distance between wells is preserved, enabling the use of multi-channel manual pipettes and robotic liquid handlers, making MRC Maxi one of the most automation-friendly optimization plates on the market.
MRC Maxi is covered by global intellectual property and design registration as are the Swissci AG MRC 2 lens 96 well plates. Several breakthrough features of the original MRC plate have been maintained. Wells are labeled individually. Drops are raised for easy access during crystal retrieval. MRC Maxi uses the same proprietary polymer specially selected for the purpose of UV light visualization and the material used keeps through-plastic evaporation to a minimum. Well shapes are spherical but shallow.
The MRC Maxi Crystallization plate offers unique properties that make it the ideal choice for microliter-sized optimization experiments and is made from UV compatible UVP.
The advantages of the MRC Maxi Crystallization plate – in brief
Easy Crystal Retrieval
Very Rigid, Automation-Friendly Plate Design
Unique Polymer (UVP)
|HR3-179||MRC Maxi 48-Well Crystallization Plate||10 plate case|
|HR3-180||MRC Maxi 48-Well Crystallization Plate||40 plate case|