Anti-6×His Polyclonal Antibody
品牌:solarbio | 货号:K007439P
| 英文名称 | Anti-6×His Polyclonal Antibody |
|---|---|
| 宿主 | Rabbit |
| 别名 | 6×His |
| 应用 | WB |
| 稀释比例 | WB 1:5000-10000. |
| 交叉反应 | Fusion proteins containing the HIS Tag |
| 蛋白分子量 | 49kDa/45 kDa |
| Gene ID | |
| 保存 | Store at -20°C. Avoid freeze / thaw cycles. |
| 储存液 | Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3. |
| 纯化方法 | Affinity purification |
| 亚型 | IgG |
| 免疫原 | A synthetic peptide |
| 性状 | 液体 |
| Public Immunogen Range | A synthetic peptide |
| 克隆类型 | Polyclonal Antibody |
| 背景资料 | The 6 X histidine motif is used as a tag on many recombinant proteins to facilitate purification.It can then be purified using immobilized metal ions,traditionally nickel,in a procedure termed immobilized-metal affinity chromatography.Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting.The antibody recognizes the His-tag fused to targeted proteins in transfected or transformed cells. |