Anti-6×His Polyclonal Antibody
品牌:solarbio | 货号:K007439P
英文名称 |
Anti-6×His Polyclonal Antibody |
宿主 |
Rabbit |
别名 |
6×His |
应用 |
WB |
稀释比例 |
WB 1:5000-10000. |
交叉反应 |
Fusion proteins containing the HIS Tag |
蛋白分子量 |
49kDa/45 kDa |
Gene ID |
|
保存 |
Store at -20°C. Avoid freeze / thaw cycles. |
储存液 |
Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3. |
纯化方法 |
Affinity purification |
亚型 |
IgG |
免疫原 |
A synthetic peptide |
性状 |
液体 |
Public Immunogen Range |
A synthetic peptide |
克隆类型 |
Polyclonal Antibody |
背景资料 |
The 6 X histidine motif is used as a tag on many recombinant proteins to facilitate purification.It can then be purified using immobilized metal ions,traditionally nickel,in a procedure termed immobilized-metal affinity chromatography.Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting.The antibody recognizes the His-tag fused to targeted proteins in transfected or transformed cells. |