Anti-6×His Polyclonal Antibody-一抗-抗体-蛋白与免疫

Anti-6×His Polyclonal Antibody

品牌:solarbio | 货号:K007439P

英文名称 Anti-6×His Polyclonal Antibody
宿主 Rabbit
别名 6×His
应用 WB
稀释比例 WB 1:5000-10000.
交叉反应 Fusion proteins containing the HIS Tag
蛋白分子量 49kDa/45 kDa
Gene ID
保存 Store at -20°C. Avoid freeze / thaw cycles.
储存液 Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
纯化方法 Affinity purification
亚型 IgG
免疫原 A synthetic peptide
性状 液体
Public Immunogen Range A synthetic peptide
克隆类型 Polyclonal Antibody
背景资料 The 6 X histidine motif is used as a tag on many recombinant proteins to facilitate purification.It can then be purified using immobilized metal ions,traditionally nickel,in a procedure termed immobilized-metal affinity chromatography.Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting.The antibody recognizes the His-tag fused to targeted proteins in transfected or transformed cells.