Anti-Flag tag Monoclonal Antibody
品牌：solarbio | 货号：K200001M
| 英文名称 | Anti-Flag tag Monoclonal Antibody |
|---|---|
| 宿主 | Mouse |
| 别名 | DDDDK Tag;DYKDDDDK Tag |
| 应用 | WB IP IF |
| 稀释比例 | WB 1:10000-15000. IP 1:100-300. IF 1:500-3000. |
| 保存 | Store at -20°C. Avoid freeze / thaw cycles. |
| 储存液 | Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3. |
| 纯化方法 | Affinity purification |
| 亚型 | IgG1 |
| 免疫原 | A synthetic peptide coupled to KLH |
| 性状 | 液体 |
| Public Immunogen Range | A synthetic peptide coupled to KLH |
| 克隆号 | 2C5 |
| 克隆类型 | Monoclonal Antibody |
| 背景资料 | The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an .. IF there is no against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an against the Flag sequence. |