Anti-Flag tag Monoclonal Antibody-一抗-抗体-蛋白与免疫

Anti-Flag tag Monoclonal Antibody

品牌:solarbio | 货号:K200001M

英文名称 Anti-Flag tag Monoclonal Antibody
宿主 Mouse
别名 DDDDK Tag;DYKDDDDK Tag
应用 WB IP IF
稀释比例 WB 1:10000-15000. IP 1:100-300. IF 1:500-3000.
保存 Store at -20°C. Avoid freeze / thaw cycles.
储存液 Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
纯化方法 Affinity purification
亚型 IgG1
免疫原 A synthetic peptide coupled to KLH
性状 液体
Public Immunogen Range A synthetic peptide coupled to KLH
克隆号 2C5
克隆类型 Monoclonal Antibody
背景资料 The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an .. IF there is no against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an against the Flag sequence.