Anti-Flag tag Monoclonal Antibody
品牌:solarbio | 货号:K200001M
英文名称 |
Anti-Flag tag Monoclonal Antibody |
宿主 |
Mouse |
别名 |
DDDDK Tag;DYKDDDDK Tag |
应用 |
WB IP IF |
稀释比例 |
WB 1:10000-15000. IP 1:100-300. IF 1:500-3000. |
保存 |
Store at -20°C. Avoid freeze / thaw cycles. |
储存液 |
Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3. |
纯化方法 |
Affinity purification |
亚型 |
IgG1 |
免疫原 |
A synthetic peptide coupled to KLH |
性状 |
液体 |
Public Immunogen Range |
A synthetic peptide coupled to KLH |
克隆号 |
2C5 |
克隆类型 |
Monoclonal Antibody |
背景资料 |
The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an .. IF there is no against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an against the Flag sequence. |