Anti-EGFR Monoclonal Antibody-一抗-抗体-蛋白与免疫

Anti-EGFR Monoclonal Antibody

品牌:solarbio | 货号:K200050M

英文名称 Anti-EGFR Monoclonal Antibody
宿主 Mouse
别名 Epidermal growth factor receptor;erbb 1;Receptor tyrosine protein kinase ErbB 1
应用 WB IF IHC
稀释比例 WB 1:500-2000. IF 1:100-200. IHC 1:200-500.
交叉反应 Human
蛋白分子量 170kDa
Gene ID 1956
保存 Store at -20°C. Avoid freeze / thaw cycles.
储存液 Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
纯化方法 Affinity purification
亚型 IgG1
免疫原 A synthetic peptide coupled to KLH
性状 液体
Public Immunogen Range A synthetic peptide coupled to KLH
Subcellular Locations Nucleus Endosome Plasma membrane Golgi apparatus Endoplasmic reticulum secreted
Swiss Prot P00533
克隆号 1B1
克隆类型 Monoclonal Antibody
背景资料 The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascadesβ-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against β-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Actin may not be stable in certain cells. For example, expression of β-Actin in adipose tissue is very low and therefore β-Actin should not be used as loading control for these tissues.