Anti-VSV-G-tag Monoclonal Antibody-一抗-抗体-蛋白与免疫

Anti-VSV-G-tag Monoclonal Antibody

品牌:solarbio | 货号:K200005M

英文名称 Anti-VSV-G-tag Monoclonal Antibody
宿主 Mouse
别名 VSV tag;VSVG tag
应用 WB IP IF
稀释比例 WB 1:10000-15000. IP 1:100-300. IF 1:500-2000.
保存 Store at -20°C. Avoid freeze / thaw cycles.
储存液 Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
纯化方法 Affinity purification
亚型 IgG1
免疫原 A synthetic peptide coupled to KLH
性状 液体
Public Immunogen Range A synthetic peptide coupled to KLH
克隆号 8D6
克隆类型 Monoclonal Antibody
背景资料 Vesicular stomatitis virus (VSV), an enveloped RNA virus from the Rhabdoviridae family, is released from the plasma membrane of host cells by a process called budding. The fusiogenic envelope G glycoprotein of the vesicular stomatitis virus (VSV-G) that has been used to pseudotype retrovirus and lentivirus vectors can be used alone as an efficient vehicle for gene transfer. VSV-G protein is secreted into the culture medium as sendimentable vesicles from cells transfected with a VSV-G expression plasmid in the absence of other viral components. The VSV-G vesicles in the conditioned medium can be partially purified by pelleting through sucrose cushion ultracentrifugation.