Anti-VSV-G-tag Monoclonal Antibody
品牌:solarbio | 货号:K200005M
英文名称 |
Anti-VSV-G-tag Monoclonal Antibody |
宿主 |
Mouse |
别名 |
VSV tag;VSVG tag |
应用 |
WB IP IF |
稀释比例 |
WB 1:10000-15000. IP 1:100-300. IF 1:500-2000. |
保存 |
Store at -20°C. Avoid freeze / thaw cycles. |
储存液 |
Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3. |
纯化方法 |
Affinity purification |
亚型 |
IgG1 |
免疫原 |
A synthetic peptide coupled to KLH |
性状 |
液体 |
Public Immunogen Range |
A synthetic peptide coupled to KLH |
克隆号 |
8D6 |
克隆类型 |
Monoclonal Antibody |
背景资料 |
Vesicular stomatitis virus (VSV), an enveloped RNA virus from the Rhabdoviridae family, is released from the plasma membrane of host cells by a process called budding. The fusiogenic envelope G glycoprotein of the vesicular stomatitis virus (VSV-G) that has been used to pseudotype retrovirus and lentivirus vectors can be used alone as an efficient vehicle for gene transfer. VSV-G protein is secreted into the culture medium as sendimentable vesicles from cells transfected with a VSV-G expression plasmid in the absence of other viral components. The VSV-G vesicles in the conditioned medium can be partially purified by pelleting through sucrose cushion ultracentrifugation. |