Anti-MGMT Polyclonal Antibody
品牌：solarbio | 货号：K006793P
| 英文名称 | Anti-MGMT Polyclonal Antibody |
|---|---|
| 宿主 | Rabbit |
| 别名 | 6-O-methylguanine-DNA methyltransferase |
| 应用 | WB |
| 稀释比例 | WB 1:200-1000. |
| 交叉反应 | Human |
| 蛋白分子量 | 22kDa |
| Gene ID | 4255 |
| 保存 | Store at -20°C. Avoid freeze / thaw cycles. |
| 储存液 | Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3. |
| 纯化方法 | Affinity purification |
| 亚型 | IgG |
| 免疫原 | Recombinant protein of human MGMT |
| 性状 | 液体 |
| Public Immunogen Range | Recombinant protein of human MGMT |
| Subcellular Locations | Nucleus |
| Swiss Prot | P16455 |
| 克隆类型 | Polyclonal Antibody |
| 背景资料 | MGMT (O6-methylguanine-DNA methyltransferase) is transcriptionally activated in response to DNA damage and functions to repair mutagenic and cytotoxic O6-alkylguanine lesions caused by carcinogens or cytostatic drugs. MGMT induction by ionising radiation does not occur in p53-deficient mice, suggesting that MGMT induction may require p53. Similarly, MGMT mRNA and protein were shown to be inducible by ionising radiation, only in cell lines that express functional p53, and not in cell lines that do not express wild type p53. In contrast, high MGMT activity was associated with the presence of mutant p53, in a study of oral cancer cell lines. Similarly, MGMT activity was significantly lower in ovarian tumors with wildtype p53 than in tumors with mutant p53, supporting the view that wildtype p53 down-regulates the basal MGMT promoter. |