Anti-PPP1R12A Polyclonal Antibody
品牌：solarbio | 货号：K004724P
| 英文名称 | Anti-PPP1R12A Polyclonal Antibody |
|---|---|
| 宿主 | Rabbit |
| 别名 | M130;MBS;MYPT1 |
| 应用 | WB IHC |
| 稀释比例 | WB 1:500-2000. IHC 1:50-200. |
| 交叉反应 | Human Mouse Rat |
| 蛋白分子量 | 115kDa |
| Gene ID | 4659 |
| 保存 | Store at -20°C. Avoid freeze / thaw cycles. |
| 储存液 | Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| 纯化方法 | Affinity purification |
| 亚型 | IgG |
| 免疫原 | Recombinant protein of human PPP1R12A |
| 性状 | 液体 |
| Public Immunogen Range | Recombinant protein of human PPP1R12A |
| Subcellular Locations | Cytoplasm |
| Swiss Prot | O14974 |
| 克隆类型 | Polyclonal Antibody |
| 背景资料 | Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene. |