Anti-PIKFYVE Polyclonal Antibody
品牌：solarbio | 货号：K004715P
| 英文名称 | Anti-PIKFYVE Polyclonal Antibody |
|---|---|
| 宿主 | Rabbit |
| 别名 | CFD;FAB1;HEL37;PIP5K;PIP5K3;ZFYVE29 |
| 应用 | WB IHC IF |
| 稀释比例 | WB 1:500-2000. IHC 1:50-100. IF 1:10-100. |
| 交叉反应 | Human Mouse Rat |
| 蛋白分子量 | 237kDa |
| Gene ID | 200576 |
| 保存 | Store at -20°C. Avoid freeze / thaw cycles. |
| 储存液 | Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| 纯化方法 | Affinity purification |
| 亚型 | IgG |
| 免疫原 | Recombinant protein of human PIKFYVE |
| 性状 | 液体 |
| Public Immunogen Range | Recombinant protein of human PIKFYVE |
| Subcellular Locations | Endosome membrane |
| Swiss Prot | Q9Y2I7 |
| 克隆类型 | Polyclonal Antibody |
| 背景资料 | Phosphorylated derivatives of phosphatidylinositol (PtdIns) regulate cytoskeletal functions, membrane trafficking, and receptor signaling by recruiting protein complexes to cell- and endosomal-membranes. Humans have multiple PtdIns proteins that differ by the degree and position of phosphorylation of the inositol ring. This gene encodes an enzyme (PIKfyve; also known as phosphatidylinositol-3-phosphate 5-kinase type III or PIPKIII) that phosphorylates the D-5 position in PtdIns and phosphatidylinositol-3-phosphate (PtdIns3P) to make PtdIns5P and PtdIns(3,5)biphosphate. The D-5 position also can be phosphorylated by type I PtdIns4P-5-kinases (PIP5Ks) that are encoded by distinct genes and preferentially phosphorylate D-4 phosphorylated PtdIns. In contrast, PIKfyve preferentially phosphorylates D-3 phosphorylated PtdIns. In addition to being a lipid kinase, PIKfyve also has protein kinase activity. PIKfyve regulates endomembrane homeostasis and plays a role in the biogenesis of endosome carrier vesicles from early endosomes. Mutations in this gene cause corneal fleck dystrophy (CFD); an autosomal dominant disorder characterized by numerous small white flecks present in all layers of the corneal stroma. Histologically, these flecks appear to be keratocytes distended with lipid and mucopolysaccharide filled intracytoplasmic vacuoles. Alternative splicing results in multiple transcript variants encoding distinct isoforms. |