Magazyme试剂盒Cellulase Assay Kit CellG5 Method K-CellG5 Schemes

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Cellulase Assay Kit (CellG5 Method) Cellulase Assay Kit CellG5 Method K-CellG5 Schemes Cellulase Assay Kit CellG5 Method K-CellG5 Schemes

Product code: K-CellG5-4V

Content:

120 / 240 assays (manual) / 480 assays (auto-analyser) 60 / 120 assays (manual) / 240 assays (auto-analyser)

€351.00

120 / 240 assays (manual) / 480 assays (auto-analyser)

Prices exclude VAT

  • Assay Protocol
  • SDS
  • Mega-Calc
  • COAs
  • Content: (K-CellG5-4V)
    120 / 240 assays (manual) / 480 assays (auto-analyser) or 
    (K-CellG5-2V)
    60 / 120 assays (manual) / 240 assays (auto-analyser)
    Shipping Temperature: Ambient
    Storage Temperature: Short term stability: 2-8oC,
    Long term stability: See individual component labels
    Stability: > 2 years under recommended storage conditions
    Analyte: endo-Cellulase
    Assay Format: Spectrophotometer, Auto-analyser
    Detection Method: Absorbance
    Wavelength (nm): 400
    Signal Response: Increase
    Limit of Detection: 1.2 x 10-3 U/mL
    Reproducibility (%): ~ 3%
    Total Assay Time: 10 min
    Application examples: Fermentation broths, industrial enzyme preparations and biofuels research.
    Method recognition: Novel method

    The K-CellG5-2V pack size has been discontinued (read more).

    Cellulase Activity Assay Kit.

    The CellG5 assay reagent for the measurement of endo-cellulase (endo-1,4-β-glucanase) contains two components; 1) 4,6-O-(3-Ketobutylidene)-4-nitrophenyl-β-D-cellopentaoside (BPNPG5) and 2) thermostable β-glucosidase. The ketone blocking group prevents any hydrolytic action by the β-glucosidase on BPNPG5.  Incubation with an endo-cellulase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary β-glucosidase. The rate of formation of 4-nitrophenol is therefore directly related to the hydrolysis of BPNPG5 by the endo-cellulase. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH 9.0).

    The CellG5 assay represents a huge step forward in the methodology for the measurement of cellulase that traditionally relied on substrates such as CM-cellulose, Avicel, cellooligosaccharides, filter paper or dyed polysaccharides including CMC Congo red or cellulose azure.

    View Cellulase Activity Assay Protocol.

    View our complete list of assay kits for enzyme activities.

    Cellulase Assay Kit CellG5 Method K-CellG5 Schemes

    Advantages
    • Very cost effective 
    • All reagents stable for > 4 years 
    • Completely specific for cellulase (endo-1,4-glucanase) 
    • Generally applicable and highly sensitive 
    • Simple format. Well suited to automation 
    • Standard included

    Related products
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    T-CCZ-200T – Cellazyme C Tablets S-ACMCL – Azo-CM-Cellulose (Liquid)

    Related Carbohydrates
    P-BGBL – β-Glucan (Barley; Low Viscosity) P-BGBM – β-Glucan (Barley; Medium Viscosity) P-BGBH – β-Glucan (Barley; High Viscosity) P-BGOM – β-Glucan (Oat; Medium Viscosity) P-BGCFA – β-Glucan CFA Standard P-GLCML – Glucomannan (Konjac; Low Viscosity) P-XYGLN – Xyloglucan (Tamarind) O-CTR-50MG – Cellotriose O-CTE-50MG – Cellotetraose O-CPE-20MG – Cellopentaose O-CHE – Cellohexaose

    Associated Enzymes
    E-CELAN – Cellulase (endo-1,4-β-D-glucanase)
    (Aspergillus niger)

    Similar Products
    T-CCZ-200T – Cellazyme C Tablets S-ACMCL – Azo-CM-Cellulose (Liquid)
    Related Carbohydrates
    P-BGBL – β-Glucan (Barley; Low Viscosity) P-BGBM – β-Glucan (Barley; Medium Viscosity) P-BGBH – β-Glucan (Barley; High Viscosity) P-BGOM – β-Glucan (Oat; Medium Viscosity) P-BGCFA – β-Glucan CFA Standard P-GLCML – Glucomannan (Konjac; Low Viscosity) P-XYGLN – Xyloglucan (Tamarind) O-CTR-50MG – Cellotriose O-CTE-50MG – Cellotetraose O-CPE-20MG – Cellopentaose O-CHE – Cellohexaose
    Associated Enzymes
    E-CELAN – Cellulase (endo-1,4-β-D-glucanase)
    (Aspergillus niger)

    Cellulase Assay Kit CellG5 Method K-CellG5 Schemes