Magazyme试剂盒Xylanase Assay Kit XylX6 Method K-XylX6 XylX6 Structure

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Xylanase Assay Kit (XylX6 Method) Xylanase Assay Kit XylX6 Method K-XylX6 XylX6 Structure Xylanase Assay Kit XylX6 Method K-XylX6 XylX6 Structure Xylanase Assay Kit XylX6 Method K-XylX6 XylX6 Structure

Product code: K-XylX6-2V

Content:

200 assays (manual) / 400 assays (auto-analyser) 100 assays (manual) / 200 assays (auto-analyser)

€351.00

200 assays (manual) / 400 assays (auto-analyser)

Prices exclude VAT

  • Assay Protocol
  • SDS
  • Mega-Calc
  • COAs
  • FAQs
    • Content: (K-XylX6-1V)
    100 assays (manual) / 200 assays (auto-analyser)
    (K-XylX6-2V)
    200 assays (manual) / 400 assays (auto-analyser)
    Shipping Temperature: Ambient
    Storage Temperature: Short term stability: 2-8oC,
    Long term stability: See individual component labels
    Stability: > 2 years under recommended storage conditions
    Analyte: endo-1,4-β-Xylanase
    Assay Format: Spectrophotometer, Auto-analyser
    Detection Method: Absorbance
    Wavelength (nm): 400,
    405  
    Signal Response: Increase
    Limit of Detection: 5.3 x 10-4 U/mL
    Reproducibility (%): ~ 3%
    Total Assay Time: 10 min
    Application examples: Fermentation broths, industrial enzyme preparations, animal feed, biofuels research, barley malt analysis.
    Method recognition: Novel method

    The K-XYLX6-1V pack size has been discontinued (read more)

    The XylX6 assay reagent for the measurement of endo-xylanase (endo-1,4-β-xylanase) contains two components; 1) 4,6-O-(3-Ketobutylidene)-4-nitrophenyl-β-D-45-glucosyl-xylopentaoside and 2) β-xylosidase. The ketone blocking group prevents any hydrolytic action by the β-xylosidase or other exo-acting glycosidases on the XylX6 substrate. Incubation with an endo-xylanase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary β-xylosidase. The rate of formation of 4-nitrophenol is therefore directly related to the hydrolysis of XylX6 by the endo-xylanase. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH = 10.0).

    Need to measure other enzyme activities? View our complete list of assay kits for measurement of enzyme activity.

    Note that standard curves relating the absorbance obtained using the XylX6 assay to endo-xylanase activity on the native substrates, wheat arabinoxylan and beechwood xylan, are provided in the Supporting Information file under the Documents tab.

    Xylanase Assay Kit XylX6 Method K-XylX6 XylX6 Structure

    Advantages
    • Very cost effective 
    • All reagents stable for > 4 years 
    • Completely specific for endo-1,4-β-xylanase 
    • Generally applicable and highly sensitive 
    • Simple format. Well suited to automation 
    • Excellent reproducibility 
    • Standard included

    Related products
    Similar Products
    K-XYLS – Xylanase Assay Kit (Xylazyme AX)

    Related Carbohydrates
    P-XYLNBE-10G – Xylan (Beechwood) P-WAXYL – Arabinoxylan (Wheat Flour; Low Viscosity) P-WAXYM – Arabinoxylan (Wheat Flour; Medium Viscosity) P-WAXYH – Arabinoxylan (Wheat Flour; High Viscosity) P-WAXYRS – Arabinoxylan (Wheat Flour; for Reducing Sugar Assays) P-WAXYI – Arabinoxylan (Wheat Flour; Insoluble) P-RAXY – Arabinoxylan (Rye Flour) O-XBI – Xylobiose O-XTR – Xylotriose O-XTE – Xylotetraose O-XPE – Xylopentaose O-XHE – Xylohexaose O-A3X – 32-α-L-Arabinofuranosyl-xylobiose (A3X) O-A2XX – 23-α-L-Arabinofuranosyl-xylotriose (A2XX) O-A23XX-30MG – 23,33-di-α-L-Arabinofuranosyl-xylotriose (A2,3XX) O-XA3XX – 33-α-L-Arabinofuranosyl-xylotetraose (XA3XX)

    Colourimetric Substrates
    T-XYZ-200T – Xylazyme Tablets T-XAX-200T – Xylazyme AX Tablets S-AWAXL – Azo-Wheat Arabinoxylan (Liquid) S-AWAXP – Azo-Wheat Arabinoxylan (Powder) S-AXBL – Azo-Xylan (Birchwood) (Liquid) S-AXBP – Azo-Xylan (Birchwood) (Powder)

    Associated Enzymes
    E-BXSR-1KU – β-D-Xylosidase (Selenomonas ruminantium)

    Similar Products
    K-XYLS – Xylanase Assay Kit (Xylazyme AX)
    Related Carbohydrates
    P-XYLNBE-10G – Xylan (Beechwood) P-WAXYL – Arabinoxylan (Wheat Flour; Low Viscosity) P-WAXYM – Arabinoxylan (Wheat Flour; Medium Viscosity) P-WAXYH – Arabinoxylan (Wheat Flour; High Viscosity) P-WAXYRS – Arabinoxylan (Wheat Flour; for Reducing Sugar Assays) P-WAXYI – Arabinoxylan (Wheat Flour; Insoluble) P-RAXY – Arabinoxylan (Rye Flour) O-XBI – Xylobiose O-XTR – Xylotriose O-XTE – Xylotetraose O-XPE – Xylopentaose O-XHE – Xylohexaose O-A3X – 32-α-L-Arabinofuranosyl-xylobiose (A3X) O-A2XX – 23-α-L-Arabinofuranosyl-xylotriose (A2XX) O-A23XX-30MG – 23,33-di-α-L-Arabinofuranosyl-xylotriose (A2,3XX) O-XA3XX – 33-α-L-Arabinofuranosyl-xylotetraose (XA3XX)
    Colourimetric Substrates
    T-XYZ-200T – Xylazyme Tablets T-XAX-200T – Xylazyme AX Tablets S-AWAXL – Azo-Wheat Arabinoxylan (Liquid) S-AWAXP – Azo-Wheat Arabinoxylan (Powder) S-AXBL – Azo-Xylan (Birchwood) (Liquid) S-AXBP – Azo-Xylan (Birchwood) (Powder)
    Associated Enzymes
    E-BXSR-1KU – β-D-Xylosidase (Selenomonas ruminantium)

    Xylanase Assay Kit XylX6 Method K-XylX6 XylX6 Structure