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α-L-Arabinofuranosidase (Aspergillus niger) 
Product code: E-AFASE
€174.00
480 Units on pNP-α-Arabf at 40oC
Prices exclude VAT
| Content: | 480 Units on pNP-α-Arabf at 40oC |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | > 4 years at 4oC |
| Enzyme Activity: | α-Arabinofuranosidase |
| EC Number: | 3.2.1.55 |
| CAZy Family: | GH51 |
| CAS Number: | 9067-74-7 |
| Synonyms: | non-reducing end alpha-L-arabinofuranosidase; alpha-L-arabinofuranoside non-reducing end alpha-L-arabinofuranosidase |
| Source: | Aspergillus niger |
| Molecular Weight: | 62,000 |
| Concentration: | Supplied at ~ 300 U/mL |
| Expression: | From Aspergillus niger |
| Specificity: | Hydrolysis of α-1,2- and α-1,3-linked L-arabinofuranose residues from arabinoxylans and branched arabinans. Hydrolyses α-1,5-linked arabino-oligosaccharides at a much lower rate. |
| Specific Activity: | ~ 32 U/mg (40oC, pH 5.5 on p-nitrophenyl-α-L-arabinofuranoside); ~ 12.6 U/mg (on sugar-beet arabinan); ~ 1.2 U/mg (on wheat arabinoxylan) |
| Unit Definition: | One Unit of α-L-arabinofuranosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-α-L-arabinofuranoside (5 mM) in sodium acetate buffer (100 mM), pH 4.0 at 40oC. |
| Temperature Optima: | 40oC |
| pH Optima: | 4 |
| Application examples: | Applications in carbohydrate and biofuels research. |
High purity α-L-Arabinofuranosidase (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
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Related Carbohydrates
O-A3X – 32-α-L-Arabinofuranosyl-xylobiose (A3X) O-A2XX – 23-α-L-Arabinofuranosyl-xylotriose (A2XX) O-A23XX-30MG – 23,33-di-α-L-Arabinofuranosyl-xylotriose (A2,3XX) O-XA3XX – 33-α-L-Arabinofuranosyl-xylotetraose (XA3XX) P-WAXYL – Arabinoxylan (Wheat Flour; Low Viscosity) P-WAXYM – Arabinoxylan (Wheat Flour; Medium Viscosity) P-WAXYH – Arabinoxylan (Wheat Flour; High Viscosity) P-WAXYRS – Arabinoxylan (Wheat Flour; for Reducing Sugar Assays) P-WAXYI – Arabinoxylan (Wheat Flour; Insoluble) P-RAXY – Arabinoxylan (Rye Flour) O-ABI – Arabinobiose O-ATR – Arabinotriose O-ATE – Arabinotetraose O-APE – Arabinopentaose O-AHE – Arabinohexaose
Colourimetric Substrates
O-PNPAF – 4-Nitrophenyl-α-L-arabinofuranoside
Associated Enzymes
E-XYLNP – endo-1,4-β-Xylanase (Neocallimastix patriciarum)
Related Carbohydrates
O-A3X – 32-α-L-Arabinofuranosyl-xylobiose (A3X) O-A2XX – 23-α-L-Arabinofuranosyl-xylotriose (A2XX) O-A23XX-30MG – 23,33-di-α-L-Arabinofuranosyl-xylotriose (A2,3XX) O-XA3XX – 33-α-L-Arabinofuranosyl-xylotetraose (XA3XX) P-WAXYL – Arabinoxylan (Wheat Flour; Low Viscosity) P-WAXYM – Arabinoxylan (Wheat Flour; Medium Viscosity) P-WAXYH – Arabinoxylan (Wheat Flour; High Viscosity) P-WAXYRS – Arabinoxylan (Wheat Flour; for Reducing Sugar Assays) P-WAXYI – Arabinoxylan (Wheat Flour; Insoluble) P-RAXY – Arabinoxylan (Rye Flour) O-ABI – Arabinobiose O-ATR – Arabinotriose O-ATE – Arabinotetraose O-APE – Arabinopentaose O-AHE – Arabinohexaose
Colourimetric Substrates
O-PNPAF – 4-Nitrophenyl-α-L-arabinofuranoside
Associated Enzymes
E-XYLNP – endo-1,4-β-Xylanase (Neocallimastix patriciarum)
