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Diaphorase (Escherichia coli) 
Product code: E-DIAEC
€0.00
1,000 Units
Prices exclude VAT
| Content: | 1,000 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | Minimum 1 year at 4oC. Check vial for details. |
| Enzyme Activity: | Other Activities |
| EC Number: | 1.8.1.4 |
| CAS Number: | 9001-18-7 |
| Synonyms: | dihydrolipoyl dehydrogenase; protein-N6 (dihydrolipoyl)lysine:NAD+ oxidoreductase |
| Source: | Escherichia coli |
| Molecular Weight: | 51,700 |
| Concentration: | Supplied at ~ 275 U/mL |
| Expression: | Recombinant from Escherichia coli |
| Specificity: | Catalyses the reaction: Tetrazolium compound + NAD+ → formazan compound + NADH + H+ |
| Specific Activity: | ~ 12 U/mg (25oC, pH 9.0) |
| Unit Definition: | One Unit of diaphorase activity is defined as the amount of enzyme required to oxidise one µmole of NADH per minute with associated reduction of INT in Tris.HCl buffer (32.3 mM), pH 9.0 at 25oC. |
| Temperature Optima: | 25oC |
| pH Optima: | 9 |
| Application examples: | Used widely in diagnostic and analytical applications. |
This product has been discontinued (read more).
High purity recombinant Diaphorase (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Looking for more enzyme products? See our complete list of analytical enzymes.
