上海金畔生物科技有限公司代理Mezazyme酶试剂盒全线产品，欢迎访问Megazyme试剂盒官网了解更多信息
Fructanase Mixture
(Ultrapure, recombinant, powder) 
20,000 Units
Prices exclude VAT
| Content: | 20,000 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Below -10oC |
| Formulation: | Supplied as a freeze-dried powder |
| Physical Form: | Powder |
| Stability: | Minimum 1 year at < -10oC. Check vial for details. |
| Enzyme Activity: | Fructanase |
| EC Number: | endo-Inulinase: 3.2.1.7, exo-Inulinase: 3.2.1.80 |
| CAZy Family: | GH32 |
| CAS Number: | 9001-57-4, 9025-67-6, 37288-56-5 |
| Synonyms: | endo-inulinase: 1-beta-D-fructan fructanohydrolase exo-inulinase: fructan β-fructosidase |
| Source: | Aspergillus niger |
| Concentration: | endo-inulinase: Supplied at ~ 100 U/mL exo-inulinase: Supplied at ~ 2,000 U/mL |
| Expression: | Recombinant from Aspergillus niger |
| Specificity: | endo-Inulinase: endo-acting hydrolysis of (2,1)-β-D-fructosidic linkages in inulin. exo-Inulinase: Hydrolysis of terminal, non-reducing β-D-fructofuranoside residues in β-D-fructofuranosides. |
| Unit Definition: | endo-Inulinase: One Unit of endo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from inulin (20 mg/mL) in sodium acetate buffer (100 mM), pH 4.5. exo-Inulinase: One Unit of exo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from kestose (5 mg/mL) in sodium acetate buffer (100 mM), at pH 4.5 at 40oC. |
| Temperature Optima: | 60oC |
| pH Optima: | 4.5 |
Fructanase, Amyloglucosidase (E-AMGDF) and Isoamylase (E-ISAMY) are used in the enzyme hydrolysis step of two validated methods for the determination of polydextrose (a low molar mass dietary fiber) in foods: AOAC method 2000.11 and Chinese GB Standard 5009.245-2016.
Components: exo-Inulinase 20,000 U (on kestose at 40oC), endo-inulinase 1000 U (on fructan at 40oC).
Note: This product has been purified to remove α-galactosidase, β-glucanase and pectinase which interfere with the use of the preparation in the measurement of fructan (AOAC Method 997.08) and in the measurement of Polydextrose (AOAC Method 2000.11).
Here is our complete list of all our CAZyme products.
Validation of Methods
