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β-Glucuronidase (Escherichia coli) 
Product code: E-BGLAEC
€222.00
500,000 Units
Prices exclude VAT
| Content: | 500,000 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In solution (Tris.HCl/NaCl/EDTA) |
| Physical Form: | Solution |
| Stability: | > 4 years at 4oC |
| Enzyme Activity: | β-Glucuronidase |
| EC Number: | 3.2.1.31 |
| CAZy Family: | GH2 |
| CAS Number: | 9001-45-0 |
| Synonyms: | β-D-glucuronoside glucuronosohydrolase; GUS |
| Source: | Escherichia coli |
| Molecular Weight: | 82,600 |
| Concentration: | Supplied at ~ 250 kU/mL |
| Expression: | Recombinant from Escherichia coli |
| Specificity: | Hydrolysis of non-reducing terminal β-D-glucuronic acid residues from glycoproteins and oligosaccharides of glycoconjugates. |
| Specific Activity: | ~ 15,000 U/mg (37oC, pH 6.8 on phenolphthalein-β-D-glucuronide); ~ 50 U/mg (37oC, pH 7.5 on pNP-β-D-glucuronide) |
| Unit Definition: | 30000 U/mg protein: One Unit of β-D-glucuronosidase activity is defined as the amount of enzyme required to release one µg of phenolphthalein per hour from phenolphthalein-β-D-glucuronide (0.5 mM) in sodium phosphate buffer (100 mM) at pH 6.8 and 37oC. 110 U/mg protein: One Unit of β-D-glucuronosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol per minute from pNP-β-D-glucuronide (1 mM) in Tris.HCl buffer (100 mM) pH 7.5 and 37oC., monitored at 410 nm. |
| Temperature Optima: | 37oC |
| pH Optima: | 6.8 |
High purity recombinant β-Glucuronidase (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Do Not Freeze/Thaw.
Looking for other enzymes? Browse our Carbohydrate Active enZYme and glycobiology enzyme products lists.
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