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3-Hydroxybutyrate dehydrogenase (prokaryote)
Product code: E-HBDH
€0.00
200 Units
Prices exclude VAT
Content: | 200 Units |
Shipping Temperature: | Ambient |
Storage Temperature: | 2-8oC |
Formulation: | In 3.2 M ammonium sulphate |
Physical Form: | Suspension |
Stability: | Minimum 1 year at 4oC. Check vial for details. |
Enzyme Activity: | Dehydrogenase |
EC Number: | 1.1.1.30 |
CAS Number: | 9028-38-0 |
Synonyms: | 3-hydroxybutyrate dehydrogenase; (R)-3-hydroxybutanoate:NAD+ oxidoreductase |
Source: | Prokaryote |
Molecular Weight: | 29,177 |
Concentration: | Supplied at ~ 100 U/mL |
Expression: | Recombinant from a Prokaryotic source |
Specificity: | Catalyses the reaction: D-3-Hydroxybutyrate + NAD+ = acetoacetate + NADH + H+ |
Specific Activity: | ~ 77.4 U/mg (25oC, pH 8.0 on D-β-hydroxybutyric acid) |
Unit Definition: | One Unit of 3-hydroxybutyrate dehydrogenase is defined as the amount of enzyme required to oxidise one µmole of D-β-hydroxybutyric acid (28.3 mM) per minute in the presence of NAD+ in Tris-HCl buffer (143 mM), pH 8.0 at 25oC. |
Temperature Optima: | 25oC |
pH Optima: | 8 |
Application examples: | Applications for the measurement of D-3-Hydroxybutyrate in the food, fermentation, wine, beverage and dairy industries. |
This product has been discontinued (read more).
High purity recombinant 3-Hydroxybutyrate dehydrogenase (prokaryote) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
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Associated Enzymes
E-FDHCB – Formate dehydrogenase (Candida boidinii) E-GALMUT – Galactose dehydrogenase – Galactose mutarotase E-HKGDH – Hexokinase/Glucose-6-phosphate dehydrogenase