- 详细信息
- 询价记录
- 相关实验
- 技术资料
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- 货号：
MBG-PMW20-5001
- 克隆性：
多克隆
可用于3D细胞培养和其他领域
PNIPAAm-PEG；聚N-异丙基丙烯酰an和聚乙二醇的嵌段共聚物
水凝胶具有聚合材料的多种特征，如网络结构和高含水量，目前他广泛的运用于医药生命科学等多种领域，但不仅限于3D培养、组织工程和药物传送这些。聚N-异丙基丙烯酰an和聚乙二醇的嵌段共聚物早在2000年初就已经商业化，研究证明，Mebiol® Gel的特性使其非常适合应用于细胞培养和组织工程。
◆原理
Mebiol Gel温敏性水凝胶与市面上其它水凝胶的不同之处在于它可以跟随温度变化进行可逆性溶胶-凝胶转换。当温度降低时，Mebiol Gel为溶胶状态（像液体），当温度较高时形成水凝胶。在实验操作中，该特性特别有利于进行细胞操作，比如可轻松向冷却的Mebiol Gel中加入培养基，可通过给培养瓶降温的方式进行细胞离心收集。在凝胶状态时，Mebiol Gel的高亲脂性为细胞增殖、细胞信号转导、气体质量交换以及细胞和组织对剪切力的防御等提供了非常有效的生长环境。
◆优点、特色
●易于操作
● 无毒性，生物相容性好
● 100％合成，无病原体
● 透明度高，利于细胞观察
● 性能完善
◆案例、应用
● 干细胞和多能干细胞培养，增殖和分化
● 3D细胞培养
● 细胞移植
● 器官和组织再生
● 药物传递
● 非细胞培养应用
1. Mebiol® Gel中培养原发性肿瘤细胞
选取人肿瘤组织中的原发性肿瘤细胞进行培养该技术能够鉴定来自患者的原发性肿瘤细胞的表征，因此可根据其主要细胞化学敏感性，恶性肿瘤，转移酶活性和其 它参数对患者的治疗进行评估。在胶原或者3D凝胶中培养原发性肿瘤细胞，纤维细胞的过度生长可能对其产生抑制作用。而成纤维细胞在Mebiol® Gel中不容易增殖，因此能选择性增殖原代肿瘤细胞，以便后续进一步分析鉴别。
图 1：人体癌变结肠组织Mebiol® Gel培养10天。
(提供者：Dr. S. Kubota, Dept. of General Surgery, St.Marianna University School of Medicine)
人结肠癌组织在Mebiol® Gel培养10天。只有原发性肿瘤细胞中Mebiol® Gel中增殖。
成纤维细胞在Mebiol® Gel中生长受到抑制，而在胶原蛋白和其它许多3D凝胶培养基质中，长满成纤维细胞，阻止癌细胞的增殖。
图 2：细胞生长曲线
2. 干细胞培养
猕猴胚胎干细胞用不含LIF的Mebiol® Gel培养（右图），和2D饲养层培养（左图）相比，发现形态学和碱性磷酸酶染色均未分化。
图 3
左图：2D 饲养层培养。
右图：不含LIF的3D Mebiol® Gel培养（7天）。
提供者：Dr. K. Hishikawa, Dept of Clinical Renal Regeneration, University of Tokyo.
猕猴（灵长类）胚胎干细胞在Mebiol Gel® 培养，碱性磷酸酶染色表现强阳性，表明未分化。
图 4
左图：2D 饲养层培养。
右图：不含LF的3D Mebiol® Gel培养（5天）。
3.球状体形成
Mebiol® Gel 可支持肿瘤细胞系和iPS细胞形成球体。
图 5：粘液表皮样癌（胆管癌）衍生细胞系在Mebiol® Gel中形成球状体。
(提供者： Dr. S. Kubota, Dept. of General Surgery, St. Marianna University School of Medicine)
4. 保持组织结构
Mebiol® Gel提供的环境，有利于维护组织结构在长期培养。
图 6
左图：正常结肠黏膜组织在Mebiol® Gel 中培养7天后。
右图：转移性肝癌组织在Mebiol® Gel 中培养21天后。
(提供者：Dr. S. Kubota, Dept. of General Surgery, St. Marianna University School of Medicine)
5. 体干细胞的选择性分离培养（小鼠胚胎皮肤源）
Epithelial Stem Cells from Dermis by a Three-dimensional Culture System” Journal of Cellular Biochemistry, 98 (1), 174-184 (2006)
6. 体外3-D软骨细胞培养再生软骨组织
Chondrocytes Containing Growth Factors in a Novel Thermoreversible Gelation Polymer Scaffold” Tissue Engineering, 12 (5), 1237-1245 (2006)
7. 体外3-D培养人细胞间质干细胞（hMSC）进行骨诱导
“Gene expression profile of human mesenchymal stem cells during osteogenesis in three-dimensional thermoreversible gelation polymer” Biochem. Biophys. Res. Commun., 317, 1103-1107 (2004).
8. 人类肝细胞3-D培养生产丙型肝炎病毒（HCV）
“Production of infectious hepatitis C virus particles in three-dimensional cultures of the cell line carrying the genome-length dicistronic viral RNA of genotype 1b“ Virology, 351 (2), 381-392 (2006)
9. 通过局部加热进行通道控制（芯片细胞分选系统）
“On-Chip Cell Sorting System Using Laser-Induced Heating of a Thermoreversible Gelation Polymer to Control Flow”, Y. Shirasaki, J. Tanaka, H. Makazu, K. Tashiro, S. Shoji, S. Tsukita, T. Funatsu,Anal. Chem., 78, 695-701 (2006)
参考文献：
细胞培养和组织再生
研究领域
Publication
Pubmed ID (PMID)
干细胞（人类多能干细胞 （hPSC）增殖和分化）
A fully defined and scalable 3D culture system for human pluripotent stem cell expansion and differentiation. Lei Y, Schaffer DV. Proc Natl Acad Sci U S A. 2013 Dec 24;110(52):E5039-48. doi: 10.1073/pnas.1309408110. Epub 2013 Nov 18.PMID: 24248365
24248365
干细胞（角膜缘），综述
Towards the use of hydrogels in the treatment of limbal stem cell deficiency Bernice Wright, Shengli Mi, Che J. Connon Drug Discovery Today, Volume 18, Issues 12, January 2013, Pages 79-86 a
22846850
细胞培养（肾囊肿的形成）
Mxi1 influences cyst formation in three-dimensional cell culture. YJ Yook, KH Yoo, SA Song, MJ Seo, JY Ko, BH Kim, EJ Lee, E Chang, YM Woo, and JH Park BMB Rep, Mar 2012; 45(3): 189-93.
22449707
基于细胞的ROS测定
Determination of Chronic Inflammatory States in Cancer Patients Using Assay of Reactive Oxygen Species Production by Neutrophils Yoko Suzuki, Satoshi Ohno, Ryuji Okuyama, Atsushi Aruga, Masakazu Yamamoto, Shigeki Miura, Hiroshi Yoshioka, Yuichi Mori, And Katsuhiko Suzuki Anticancer Res, Feb 2012; 32: 565 – 570.ROS Cell-Based Assay
22287746
基于细胞的ROS测定
Effect of Green Tea Extract on Reactive Oxygen Species Produced by Neutrophils from Cancer Patients Katsuhiko Suzuki, Satoshi Ohno, Yoko Suzuki, Yumiko Ohno, Ryuji Okuyama, Atsushi Aruga, Masakazu Yamamoto, Ken-O Ishihara, Tsutomu Nozaki, Shigeki Miura, Hiroshi Yoshioka, And Yuichi Mori Anticancer Res, Jun 2012; 32: 2369 – 2375.
22641677
干细胞培养，再生医学
Application of a Thermo-Reversible Gelation Polymer, Mebiol Gel, for Stem Cell Culture and Regenerative Medicine Kataoka K and Huh N*Journal of Stem Cell & Regenerative Medicine 2010 Vol. 6(1): p10-14 (2010)
link
器官培养
FGF signaling directs a center-to-pole expansion of tubulogenesis in mouse testis differentiation. Hiramatsu R, Harikae K, Tsunekawa N, Kurohmaru M, Matsuo I, Kanai Y. Development. 2010 Jan;137(2):303-12. doi: 10.1242/dev.040519.
20040496
干细胞（角膜缘）
Ex vivo cultivation of corneal limbal epithelial cells in a thermoreversible polymer (Mebiol Gel) and their transplantation in rabbits: an animal model. G Sitalakshmi, B Sudha, HN Madhavan, S Vinay, S Krishnakumar, Y Mori, H Yoshioka, and S Abraham Tissue Eng Part A, Feb 2009; 15(2): 407-15.
18724830
干细胞（角膜缘）
Limbal Stem Cells: Application in Ocular Biomedicine Review Article Geeta K. Vemuganti, Anees Fatima, Soundarya Lakshmi Madhira, Surendra Basti, Virender S. Sangwan International Review of Cell and Molecular Biology, Volume 275, 2009, Pages 133-181
19491055
病毒感染/复制系统
3D cultured immortalized human hepatocytes useful to develop drugs for blood-borne HCV Hussein Hassan Aly, Kunitada Shimotohno, Makoto Hijikata Biochemical and Biophysical Research Communications, Volume379, Issue 2, 6 February 2009, Pages 330-334
19103167
胚胎移植培养
Antagonism between Smad1 and Smad2 signaling determines the site of distal visceral endoderm formation in the mouse embryo.Yamamoto M, Beppu H, Takaoka K, Meno C, Li E, Miyazono K, Hamada H. J Cell Biol. 2009 Jan 26;184(2):323-34. doi: 10.1083/jcb.200808044. Epub 2009 Jan 19.
19153222
肝细胞移植
Intraperitoneal Transplantation Of Hepatocytes Embedded In Thermoreversible Gelation Polymer (Mebiol Gel) In Acute Liver Failure Rat Model. N. Parveen, A.A. Khan, S. Baskar, M.A. Habeeb, P. Ravindra Babu, A. Samuel, Y. Hiroshi, M. Yuichi, C.M. Habibullah Hepatitis Monthly, Volume 275 8, Issue 4, Autumn, November 2008 Page S71
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干细胞（间充质）
Chrondrogenic differentiation of human mesenchymal stem cells from umbilical cord blood in chemicially synthesized thermoreversible polymer. Kao, I, et al. Chinese J. Physiology, 51(4), 252-258 (2008)
19112883
肝细胞培养
Serum-derived hepatitis C virus infectivity in interferon regulatory factor-7-suppressed human primary hepatocytes. Hussein H. Aly, Koichi Watashi, Makoto Hijikata, Hiroyasu Kaneko, Yasutugu Takada, Hiroto Egawa, Shinji Uemoto, Kunitada Shimotohno Journal of Hepatology, Volume 46, Issue 1, January 2007, Pages 26-36
17112629
干细胞（角膜缘）
Cultivation of human corneal limbal stem cells in Mebiol gel–A thermo-reversible gelation polymer. B Sudha, HN Madhavan, G Sitalakshmi, J Malathi, S Krishnakumar, Y Mori, H Yoshioka, and S Abraham Indian J Med Res, Dec 2006; 124(6): 655-64
17287553
组织工程（骨）
In vitro culture of chondrocytes in a novel thermoreversible gelation polymer scaffold containing growth factors. Yasuda A, Kojima K, Tinsley KW, Yoshioka H, Mori Y, Vacanti CA. Tissue Eng. 2006 May;12(5):1237-45.
16771637
干细胞（上皮）
Isolation of epithelial stem cells from dermis by a three-dimensional culture system. Medina RJ, Kataoka K, Takaishi M, Miyazaki M, Huh NH. J Cell Biochem. 2006 May 1;98(1):174-84.
16408300
干细胞（角膜缘）
Comparative Study on Growth Characteristics of Cadaveric Human Corneal Limbal Stem Cells in Mebiol Gel (a Synthetic Polymer) and on Human Amniotic Membrane. H.N. Madhavan, B. Sudha1, G. Sitalakshmi, S. KrishnaKumar, Y. Mori, H. Yoshioka and S. Abraham.Invest Ophthalmol Vis Sci 2006;47: E-Abstract 3033. 3033B186
组织再生（肝）
Thermoreversible gelation polymer induces the emergence of hepatic stem cells in the partially injured rat liver. Nagaya M, Kubota S, Suzuki N, Akashi K, Mitaka T.Hepatology. 2006 May;43(5):1053-62.
16628635
病毒增殖和药物筛选
Production of infectious hepatitis C virus particles in three-dimensional cultures of the cell line carrying the genome-length dicistronic viral RNA of genotype 1b.Murakami K, Ishii K, Ishihara Y, Yoshizaki S, Tanaka K, Gotoh Y, Aizaki H, Kohara M, Yoshioka H, Mori Y, Manabe N, Shoji I, Sata T, Bartenschlager R, Matsuura Y, Miyamura T, Suzuki T.Virology. 2006 Aug 1;351(2):381-92. Epub 2006 May 6.
16678876
胚胎培养
Canonical Wnt Signaling and Its Antagonist Regulate Anterior-Posterior Axis Polarization by Guiding Cell Migration in Mouse Visceral Endoderm. Chiharu Kimura-Yoshida, Hiroshi Nakano, Daiji Okamura, Kazuki Nakao, Shigenobu Yonemura, Jose A. Belo, Shinichi Aizawa, Yasuhisa Matsui, Isao Matsuo Developmental Cell, Volume 9, Issue 5, November 2005, Pages 639-650
16256739
细胞在Mebiol Gel中的生长评估
H.N. Madhavan, J. Malathi, Patricia Rinku Joseph, Mori Yuichi, Samuel JK Abraham and Hiroshi Yoshioka. A study on the growth of continuous culture cell lines embedded in Mebiol Gel., Current Science, 87(9), 1275~77(2004).
干细胞培养和分化
Gene expression profile of human mesenchymal stem cells during osteogenesis in three-dimensional thermoreversible gelation polymer. Hishikawa K, Miura S, Marumo T, Yoshioka H, Mori Y, Takato T, Fujita T. Biochem Biophys Res Commun. 2004 May 14;317(4):1103-7.
15094382
肝再生
Evaluation of Thermoreversible gelation polymer for Regeneration of Focal Liver Injury. M. Nagaya, S. Kubota, N. Suzuki, M. Tadakoro, K. Akashi. Eur Surg Res, 36:95-103 (2004).
15007262
球状培养（肿瘤）
S. Tsukikawa, H. Matsuoka, Y. Kurahashi, Y. Konno, K. Satoh, R. Satoh, A. Isogai, K. Kimura, Y. Watanabe, S. Nakano, J. Hayashi, and S. Kubota. A new method to prepare multicellular spheroids in cancer cell lines using a thermo-reversible gelation polymer, Artifcial Organs, 27(7), 598 -604(2003).
12823414
伤口康复
Wound Dressing of Newly Developed Thermo gelling Thermo reversible Hydro gel. H. Yoshioka, Y. Mori, S. Kubota , Jpn J Artif Organs, 27(2), 503 -506 (1998).(Japanese Publication- Abstract in English)
胰岛移植
In Vitro Studies on a New Method for Islet Micro encapsulation Using a Thermo reversible Gelation Polymer, N-Isopropylacrylamide-Based Copolymer. S. Shimizu, M. Yamazaki, S. Kubota, T. Ozasa, H. Moriya, K. Kobayashi, M. Mikami, Y. Mori and S. Yamaguchi. Artif Organs, Vol. 20, No.11 (1996).
8908335
Mebiol Gel应用——非细胞培养
研究领域
Publication
Pubmed ID (PMID)
蛋白质结晶支架
A Novel Approach for Protein Crystallization by a Synthetic Hydrogel with Thermoreversible Gelation Polymer. Sugiyama, et al., Cryst. Growth Des., 2013, 13(5), pp 1899-1904
link
DNA电泳和修复支架
Separation and recovery of DNA fragments by electrophoresis through a thermoreversible hydrogel composed of poly (ethylene oxide) and poly (propylene oxide). Yoshioka H, Mori Y, Shimizu M. Anal Biochem. 2003 Dec 15;323(2):218-23.
14656528
细胞分选
On-chip cell sorting system using laser-induced heating of a thermoreversible gelation polymer to control flow. Shirasaki Y, Tanaka J, Makazu H, Tashiro K, Shoji S, Tsukita S, Funatsu T. Anal Chem. 2006 Feb 1;78(3):695-701.
16448041
细胞分选
Microfluidic cell sorter with flow switching triggered by a solgel transition of a thermo-reversible gelation polymer.Kazuto Ozaki, Hirokazu Sugino, Yoshitaka Shirasaki, Tokihiko Aoki, Takahiro Arakawa, Takashi Funatsu, Shuichi Shoji Sensors and Actuators B: Chemical, Volume 150, Issue 1, 21 September 2010, Pages 449-455
link
DNA分子分选
Microfluidic active sorting of DNA molecules labeled with single quantum dots using flow switching by a hydrogel solgel transition. Mai Haneoka, Yoshitaka Shirasaki, Hirokazu Sugino, Tokihiko Aoki, Takahiro Arakawa, Kazuto Ozaki, Dong Hyun Yoon, Noriyuki Ishii, Ryo Iizuka, Shuichi Shoji, Takashi FunatsuSensors and Actuators B: Chemical, Volume 159, Issue 1, 28 November 2011, Pages 314-320
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药物输送
Novel drug delivery system using thermoreversible gelation polymer for malignant glioma.Arai T, Joki T, Akiyama M, Agawa M, Mori Y, Yoshioka H, Abe T. J Neurooncol. 2006 Mar;77(1):9-15.
16292493
药物输送
Novel local drug delivery system using thermoreversible gel in combination with polymeric microspheres or liposomes.Arai T, Benny O, Joki T, Menon LG, Machluf M, Abe T, Carroll RS, Black PM.Anticancer Res. 2010 Apr;30(4):1057-64.
20530409
细胞培养药物筛选
Alternatives to Animal Testing and Experimentation Wakui et al. in vitro Thermoreversible Gel Disc Quantitative Assay of Rat Angiogenesis. AATEX16(2), 59-65, 2011
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Mebiol Gel 物理特性
研究领域
Publication
Pubmed ID (PMID)
物理特性
A synthetic hydrogel with thermoreversible gelation. II. : Effect of added salts. H. Yoshioka, M. Mikami, Y. Mori, and E. Tsuchida. J. Macromol. Sci., A31(1), 121-125 (1994).
link
物理特性
Thermoreversible gelation on heating and on cooling of an aqueous gelatin-poly(N-isopropylacrylamide) conjugate. , H. Yoshioka, Y. Mori, S. Tsukikawa, and S. Kubota, Polym. Adv. Tech., 9, 155-158 (1998).
link
物理特性
A Synthetic hydrogel with thermoreversible gelation. I. Preparation and rheological properties , H. Yoshioka, M. Mikami and Y. Mori. J.M.S- Pure Appl. Chem., A31(1), pp. 113-120 (1994).
link
物理特性
Preparation of Poly (N-Isopropylacrylamide)-b-Poly(Ethylene Glycol) and Calorimetric Analysis of its Aqueous Solution , H. Yoshioka, M. Mikami and Y. Mori,J.M.S- Pure Appl. Chem., A31(1), pp. 109-112 (1994).
link
物理特性
A synthetic hydrogel with thermoreversible gelation. III. : An NMR study of the Sol-Gel transition. H. Yoshioka, Y. Mori and James A. Cushman. Polym. Adv. Tech., 5, pp. 122-127 (1994).
link
产品编号 产品名称 产品规格 MBG-PMW20-1001 Mebiol ® Gel
温敏性水溶胶1*10mL MBG-PMW20-1005 Mebiol ® Gel
温敏性水溶胶5*10mL MBG-PMW20-5001 Mebiol ® Gel
温敏性水溶胶1*50mL MBG-PMW20-5005 Mebiol ® Gel
温敏性水溶胶5*50mL MBG-PMW20-5020 Mebiol ® Gel
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