α淀粉酶SD检测试剂盒（高感光度法） α-Amylase SD Assay Kit (High Sensitivity Method) 货号：K-AMYLSD Megazyme中国代理商

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α淀粉酶SD检测试剂盒（高感光度法）

英文名：α-Amylase SD Assay Kit (High Sensitivity Method)

货号：K-AMYLSD

规格：160 / 320 assays (manual) / 640 assays (auto-analyser)

The Amylase SD Method: A highly sensitive colourimetric method for the determination of α-amylase in sprout damaged wheat grain (also known as pre-harvest sprouting or weather damaged wheat grain) and “late maturity α-amylase” wheat grain.

Advantages

  • Extremely high sensitivity – 2.4-fold increase over Ceralpha (K-CERA)
  • Very cost effective
  • All reagents stable for > 2 years after preparation
  • Very specific
  • Simple format
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included

  • Suitable for Maual and auto-analyser formats

Highly sensitive colourimetric method for the determination of α-Amylase in sprout damaged grain

Principle:
(α-amylase)
(1) Ethylidene-G7-α-PNP + H2O → Ethylidene-GX + G(7-X)-α-PNP

(thermostable α-glucosidase)
(2) G(7-X)-α-PNP + H2O → D-glucose + PNP

(alkaline solution)
(3) PNP → phenolate ion (yellow colour)
Note: PNP = 4-nitrophenol

Kit size:                           160 / 320 assays (manual) / 640 (auto-analyser)
Method:                           Spectrophotometric at 400 nm
Total assay time:             ~ 5 min
Detection limit:                0.05 U/mL
Application examples:     Sprout damaged wheat grain
Method recognition:        Novel method

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