In most applications involving immobilization of living cells or other biological materials, the bead size needs to be small (<1 mm) and carefully controlled because of diffusion limitations of nutrients within hydrogel beads. An excellent method of production for small alginate beads in a controllable manner is the use of an electrostatic bead generator. The basic principle of the instrument is the use of an electrostatic force to pull droplets from a needle tip into a gelling bath. The Nisco Instrument is designed for research and production of small quantities of spherical alginate beads ranging in size from 200 to 2400 μm.

The Bead Generator consists of a Power Unit of 0-10 kV, with a switch for fine-tuning the voltage magnitude, an autoclavable needle holder and an electrical safety cage. The alginate (or other) solution is fed into the instrument with a syringe pump. A magnetic stirrer is placed underneath the gelling bath to keep the beads separated during gelling via a magnetic stir bar. Needles used in the instrument are made of stainless steel, and are available in different diameters. The instrument is shipped with needles of 0.17 mm and 0.40 mm inner diameter. The instrument is designed to produce up to 2’400 capsules/minute with a Single Nozzle and approximately 24’000 capsules/minute with a Multiple 10 Nozzle configuration with the production rate depending on the matrix and other physical parameters. On average, the standard deviation of the bead diameters is 3-6%. The bead generator has been used successfully in several studies involving immobilization of living cells.

Principle of electrostatic bead generator:

Bead size will be dependent on the following parameters: voltage, distance between the needle tip and gelling bath, solution viscosity, flow rate of the solution and needle diameter. The average distance between the needle tip and the gelling bath for this instrument is 10 mm and the bead size is determined by adjusting the voltage. The bead diameter will decrease with smaller needle diameter and increasing voltage.

To complete the System, a syringe and syringe pump (or equivalent) is required (not included).

Included with the System are:

2 nozzles (Ø 0.17 / 0.40mm)

Beakers

Magnetic stirrer

Silicon Tubing

Available Options:

Syringe Pump

Peristaltic Pump

 

微囊制备系统：科技新探索

应用：

组织工程和再生医学（同种异体和异种的组织移植、肝细胞的去毒的免疫隔离)

纳米材料

动物细胞、生物技术、食品技术和滋补药 (其生产过程中的酶、催化剂和微生物固定，例如生物转化、蛋白生产)

环境应用（废水和土壤用固定化微生物进行处理）

制药（药物的缓释、接种疫苗和益生菌运输）

化妆品和卫生（香精和香水的包囊化）

植物细胞、农业（人工种子生产、低温保藏、生物农药生产）

印刷、染料纺织、等离子喷镀等更多的领域。

性能指标：

1．单分散微珠 2. 产量：从克至公斤级 3.微珠直径：20um至2 mm  4.执行标准：cGMP和FDA

系统按工作原理分类：

薄层状间断喷射/电磁驱动ED-continuous		静电/高压驱动EV

 

同轴气流驱动EJS	空气动力驱动EJA	重力驱动EW

 

系统按单个分散珠子大小分类：

 

单个分散珠子大小μm	生产批量能力	型号	型号	工作原理	喷头	工作模式	人民币底价（万）
<10	20 ml	EVAR-J30	EJA	同轴气流驱动	单喷头	开放型	25
>200	20 ml	EVAR-V1	EV1	静电/高压驱动	单喷头	开放型	48
>200	20至60 ml	EVAR-D	ED	薄层状间断喷射/电磁驱动	单喷头	开放型	60
>200	280 ml	EVAR-B	EB	薄层状间断喷射/电磁驱动	单喷头	封闭型	60
>200	250 ml	EVAR-D-thermal	ED-thermal	薄层状间断喷射/电磁驱动	单喷头	开放型	70
>200	1至2L	EVAR-E	EE	薄层状间断喷射/电磁驱动	多喷头	开放型	90
>200	1至2L	EVAR-C	EC	薄层状间断喷射/电磁驱动	多喷头	封闭型	90
>200	2 L	EVAR-E-thermal	EE-thermal	薄层状间断喷射/电磁驱动	多喷头	开放型	90
>200	连续	EVAR-D-continuous	ED-continuous	薄层状间断喷射/电磁驱动	单喷头	开放型	60
400-500	20ml	EVAR-J1	EJS	同轴气流驱动	单喷头	开放型	5
>1500	1至2L	EVAR-W10	EW	重力驱动	多喷头	开放型	30