SureCoat, Bovine Collagen, Solution, 3 mg/mL

货号：BM-5057-100ML

品牌： Advanced Biomatrix

报价：￥3200.00

商品描述

SureCoat® collagen is provided for coating surfaces and is not intended for gel applications. SureCoat® is highly purified (>99.9% collagen content) and has excellent coating functionality and properties. SureCoat® is isolated from bovine hides sourced from the only controlled, closed herd in the United States. Advanced BioMatrix’s manufacturing processes comply with stringent quality standards that have proven to yield unsurpassed lot-to-lot consistency.

SureCoat® collagen is approximately 97% Type I collagen with the remainder being comprised of Type III collagen. It contains a high monomer content as measured by gel permeation chromatography.

SureCoat® collagen is supplied at approximately a 3 mg/ml concentration. The concentration for each specific lot is provided on a Certificate of Analysis that is available with the purchase of each product. SureCoat™ is soluble atelo-collagen in 0.01 N HCI, therefore, the pH is approximately 2.0.

SureCoat® collagen is ideal for coating of surfaces and providing a thin layer for culturing cells. SureCoat® is not intended to be used for the formation of a gel. SureCoat® collagen is provided in user-friendly packaging for use and storage. This product is sterile filtered and is supplied as a ready to use solution.

Parameter, Testing, and Method	*SureCoat® Collagen Solution* Catalog # 5057-100ML
Sterilization Method	Filtration
Form	Solution
Package Size	100 mL
Storage Temperature	2-10 °C
Expiration Date	Listed on product label and Certificate of Analysis
Concentration (Biuret Protein Determination)	2.9-3.2 mg/mL
Purity (collagen concentration)	> 99.9%
pH	Approx. pH 2, 0.01 N HCl
Polyacrylamide Gel Electrophoresis (SDS – PAGE)	> 85% collagen containing within alpha, beta, and gamma bands < 15% collagen contained with bands traveling faster than alpha
Sterility	No growth
Endotoxin (LAL)	< 1.0 EU/ml
Cell Attachment Assay	Pass
Source	Bovine Hide – Pepsin Extracted

Preparation and Usage

Coating Procedure

Note: Use these recommendations as guidelines to determine the optimal coating conditions for your culture system.

Transfer desired volume of collagen solution from the bottle to a dilution vessel as required. Dilute to desired concentration using sterile 0.01 N HCl solution. A typical working concentration may range from 10 to 100 ?g/ml.
Add appropriate amount of diluted SureCoat® material to the culture surface.
Incubate at room temperature or 37°C, covered, for 1-2 hours.
After incubation, aspirate any remaining material.
Rinse coated surfaces carefully with sterile medium or PBS, avoid scratching surfaces.
Coated surfaces are ready for use. They may also be stored at 2-8°C damp or air dried if sterility is maintained.

Cell Attachment Bioassay

To demonstrate cell attachment, cells were seeded onto surfaces coated with the matrix product and onto negative control surfaces without the matrix product. Cells were seeded onto surfaces using serum-free DMEM media. All surfaces were blocked with a solution containing 1% BSA. Cells were then allowed to attach for one (1) hour at 37°C. The culture surfaces were washed with DPBS. The results indicate significant cell attachment with surfaces coated using the matrix product.

Without UltraPure®	With UltraPure®

Human Umbilical Vein Endothelial Cells (HUVEC) 10X

VERO Epithelial Cells 10X

Human Dermal Fibroblast Cells 10X

MDCK Epithelial Cells 10X