CD274, Human, Recombinant, 0.1 mg
英文名:CD274, Human, Recombinant, 0.1 mg
货号:BM-5126-0.1MG
规格:0.1 mg
品牌: Advanced Biomatrix
报价:¥2050.00
商品描述
Human CD274 (programmed cell death 1 ligand 1) is a cell membrane protein which is involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production. Recent data indicated that cancer cells that express PDL1 promote tumor progression through inhibition of PD1-expressing immune effectors. In addition, PDL1 modulates cell-mediated immunity in the infectious disease setting.
Recombinant human CD274 extracellular domain cDNA (19-238 aa, derived from BC074984) was constructed with codon optimization and expressed with a small T7-His-TEV cleavage site Tag (29aa) fusion at its N-terminal and expressed in E. coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified.
Parameter, Testing, and Method |
CD274, Human, Recombinant |
Quantity |
0.1 mg (100 µg/vial) |
Volume |
0.2 mL |
Concentration |
0.5 mg/mL |
Purity |
>90% as measured by SDS PAGE |
Formulation |
Formulated in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, L-arginine, DTT and Glycerol |
Form |
Solution |
Production Type |
Recombinant – E. coli |
Storage Temperature |
Keep at -20°C for long term storage. Product is stable at 4 °C for at least 30 days |
Shelf Life |
12 months after receipt |
Sterilization Method |
Filtration |
Cell Attachment Activity |
Passes |
Sterility |
No growth |
Accession Number |
NP_054862.1 |
Recombinant Protein Sequence |
MASMTGGQQMGRGHHHHHHGNLYFQG^GEFFTVTVPKDLYV VEYGSNMTIECKFPVEKQLDLAALIVYWEMEDKNIIQFVHGEE DLKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRC MISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQA EGYPKAEVIWTSSDHQVLSGKTTTTNSKREEKLFNVTSTLRIN TTTNEIFYCTFRRLDPEENHTAELVIPELPLAHPPNER |
References Downloads