A thermostable β-galactosidase that hydrolyses terminal, non-reducing β-D-galactose residues in β-D-galactosides. From the thermophilic bacterium Thermus brockianus.

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Mode of action: The enzyme hydrolyses terminal, non-reducing b-D-galactose residues in b-D-galactosides.

Unit Definition: One unit (U) is defined as the amount of enzyme which  liberates 1 mmol of p-nitrophenol per min from pNP-b-galactoside, under  the given assay conditions.

Unit Assay Conditions:  The standard assay for cellulase activity was done by incubating the enzyme at 80°C for 10 min, with 1 % (w/v) pustulan, as a substrate in 0.1M sodium phosphate buffer, pH 5,5. The reducing sugars released were detected by the dinitrosalicylic acid method using glucose as standard.