Phosphorylation plays a critical role in the regulation of many cellular functions. Common phosphorylation detection methods include expensive and often unavailable phospho-antibodies or unsafe radioactive ATP labeling. We offer pIMAGO – a novel, non-antibody, highly effective and safe product for unbiased universal detection of any phosphoprotein. Unlike antibodies, pIMAGO is capable of selective binding to a phosphorylated residue independent of amino acid sequence. This pIMAGO kit enables the detection of phosphoproteins in Western Blot format (on-membrane) using chemiluminescence (HRP/peroxidase). The kit includes pIMAGO-biotin reagent, avidin-HRP (avidin-peroxidase) detection reagent, blocking buffer, pIMAGO buffer, washing buffer and a control phosphoprotein. ECL substrate is not included.