VitroCol, Human Collagen, Solution, 3 mg/mL

英文名：VitroCol®, Human Collagen, Solution, 3 mg/mL, 20 mL

货号：BM-5007-20ML

品牌： Advanced Biomatrix

报价：￥4200.00

商品描述

VitroCol® collagen is the first widely available, naturally produced purified human collagen for research purposes. VitroCol® sets the standard for purity (>99.9% collagen content), functionality and represents the only native-like human collagen offered.

VitroCol® collagen is naturally secreted from human neo-natal fibroblast cells. The human fibroblasts are cultured in optimal conditions allowing the fibroblast to naturally and efficiently secret extracellular matrix. The extracellular matrix is then processed and purified to yield the naturally produced human collagen.

VitroCol® is approximately 97% Type I human collagen with the remainder being comprised of Type III collagen. It contains a high monomer content as measured by gel permeation chromatography.

VitroCol® is supplied at approximately 3 mg/ml concentration. The concentration for each specific lot is provided on a Certificate of Analysis that is available with the purchase of each product. VitroCol® is soluble atelo-collagen in 0.01 N HCI, therefore, the pH is approximately 2.0.

VitroCol® is especially ideal for human cell culture systems when coating of surfaces, providing preparations of thin layers of culturing cells, or use as a solid gel. VitroCol® human collagen is provided in user-friendly packaging for use and storage. VitroCol® is sterile filtered and is supplied as a ready to use solution.

Vitrocol胶原蛋白是第一个被广泛用来实验研究的天然纯化的人胶原蛋白。Vitrocol设置了标准纯度（＞99.9%胶原蛋白含量），其代表并提供的是原生胶原蛋白。Vitrocol胶原蛋白是自然分泌的人类新生儿的成纤维细胞。人成纤维细胞在最佳条件下使成纤维细胞的自然和有效的细胞外基质培养。然后经过细胞外基质进行纯化产生的胶原蛋白。Vitrocol胶原蛋白是由约97%的I型胶原蛋白，其余的为III型胶原蛋白组成的，它具有很高的单体含量可以用于测试凝胶渗透色谱。本产品的浓度约为3mg/ml，每个批次的具体浓度会写于产品的COA上随货发出。Vitrocol是溶于0.01N盐酸的端胶原蛋白，因此它的PH值约为2。Vitrocol胶原蛋白是理想的人类细胞培养系统涂层，可用于准备培养的细胞或者固体凝胶。Vitrocol胶原蛋白的包装便于储存和使用，经过灭菌的溶液随时可以使用。

Parameter, Testing, and Method	VitroCol® Collagen Solution Catalog #5007-20ML
Form	Solution
Package Size	20 mL
Sterilization Method	Filtration
Storage Temperature	2 to 10°C
Expiration Date	Listed on product label and Certificate of Analysis
Concentration (Biuret Protein Determination)	2.9-3.2 mg/ml
Purity (collagen concentration)	> 99.9%
pH	Approx. pH 2, 0.01 N HCl
Gel Time (Gel Time Assay)	< 90 minutes
Fibrillogenesis (Fibril Formation)	>0.5 Absorbance Units
Polyacrylamide Gel Electrophoresis (SDS – PAGE)	> 85% collagen containing within alpha, beta, and gamma bands < 15% collagen contained with bands traveling faster than alpha
Sterility	No growth
Endotoxin (LAL)	< 1.0 EU/ml
Cell Attachment Assay	Pass
Source	Human, Neo-Natal, Fibroblasts

Preparation and Usage

Coating Procedure

Note: Use these recommendations as guidelines to determine the optimal coating conditions for your culture system.

Transfer desired volume of collagen solution from the bottle to a dilution vessel as required. Dilute to desired concentration using sterile 0.01 N HCl solution. A typical working concentration may range from 10 to 100 ug/ml.
Add appropriate amount of diluted VitroCol® material to the culture surface.
Incubate at room temperature or 37°C, covered, for 1-2 hours.
After incubation, aspirate any remaining material.
Rinse coated surfaces carefully with sterile medium or PBS, avoid scratching surfaces.
Coated surfaces are ready for use. They may also be stored at 2-8°C damp or air dried if sterility is maintained.

3-D Gel Preparation Procedure

Slowly add 1 part of chilled 10X PBS of 10X culture media to 8 parts of chilled collagen solution with gentle swirling.
Adjust pH of mixture to 7.2-7.6 using sterile 0.1M NaOH. Monitor pH adjustment carefully (pH meter, phenol red, or pH paper)
Adjust final volume to a total of 10 parts with sterile water.
To prevent gelation, maintain temperature of mixture at 2 – 10°C.
To form gel, warm to 37°C. Allow approximately 90 to 120 minutes for gel formation.

Cell Attachment Bioassay

To demonstrate cell attachment, cells were seeded onto surfaces coated with the matrix product and onto negative control surfaces without the matrix product. Cells were seeded onto surfaces using serum-free DMEM media. All surfaces were blocked with a solution containing 1% BSA. Cells were then allowed to attach for one (1) hour at 37°C. The culture surfaces were washed with DPBS. The results indicate significant cell attachment with surfaces coated using the matrix product.

Without UltraPure®	With UltraPure®

Human Umbilical Vein Endothelial Cells (HUVEC) 10X

VERO Epithelial Cells 10X

Human Dermal Fibroblast Cells 10X

MDCK Epithelial Cells 10X