PureCol in DMEM/F-12 Medium, 5 mg/ml (0.5%), 35 mL PureCol® EZ Gel, Bovine Collagen, DMEM/F-12 Solution 货号：BM-5074-35ML 品牌：Advanced Biomatrix

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PureCol in DMEM/F-12 Medium, 5 mg/ml (0.5%), 35 mL

英文名：PureCol® EZ Gel, Bovine Collagen, DMEM/F-12 Solution

货号：BM-5074-35ML

规格：35ml

品牌： Advanced Biomatrix

报价：￥4000.00

商品描述

PureCol® EZ Gel is a ready-to-use collagen solution that forms a firm gel by simply warming to 37°C in an incubator.  The product consists of purified Type I collagen at a concentration of 0.5%, (~5 mg/ml), DMEM/F-12 medium and a mixture of L-glutamine and dipeptide (L-alanine-L-glutamine) to provide a long-lasting L-glutamine source for cell culture.

PureCol® EZ Gel is designed to improve gel consistency by pre-formulation of the medium and adjustment of the product to a neutral pH. This product avoids the inconsistencies in the preparation of the gel that can arise through variables of reagent addition, pH adjustment and handling conditions.

PureCol® EZ Gel is ideal for providing a firm gel and can also be used in the preparation of a thin layer for culturing cells.  PureCol® EZ Gel collagen is provided in a user-friendly packaging for use and storage. This product is sterile and supplied as a ready to use solution.

PureCol EZ凝胶是一种随时可以使用的胶原蛋白溶液，只要放入37°C的孵育箱中培养，其形态就会变成坚固的凝胶。本产品由纯化的、浓度为0.5%, (~5 mg/ml)的I形胶原蛋白，DMEM/F-12 培养基和L -谷氨酰胺二肽混合物（L -丙氨酸和谷氨酰胺）组成，提供了长期维持谷氨酰胺源细胞的培养。PureCol EZ凝胶的设计目的是为了通过培养基的预实验提高凝胶稠度、以及调节产品的中性PH。本产品避免了在制备凝胶通过加入试剂，调整pH值和处理条件中可能出现不一致。PureCol EZ凝胶胶原蛋白溶液是理想的细胞培养凝胶，可用于准备培养的细胞或者固体凝胶。PureCol EZ凝胶胶原蛋白溶液的包装便于储存和使用，经过灭菌的溶液随时可以使用。 

 

 

产品信息
PureCol® EZ 凝胶胶原蛋白溶液
货号 # BM-5074-35ML
形态
溶液
包装尺寸
35 mL
储存温度
2-10 °C
有效期
详见产品包装与随货发出的COA
浓度（缩二脲蛋白测定）
˜ 5 mg/ml (0.5%)
pH
pH 约为6.9 至7.4
凝胶时间（凝胶时间测定法）
< 40 分钟
无菌的
没有细菌生长
内毒素 (LAL)
<1.0 EU/mL
细胞附着实验
通过
胶原蛋白来源
牛皮–胃蛋白酶提取
培养基的补充
DMEM/F-12 培养基
谷氨酰胺来源
L -谷氨酰胺和二肽混合物（L -丙氨酸- L -谷氨酰胺）

Preparation and Usage

3-D Preparation Procedure

  1. Remove PureCol® EZ Gel from 2–10°C storage. Important: To prevent gelation, maintain temperature of product at 2–10°C.
  2. Introduce PureCol® EZ Gel into cell culture system. Cells can be added to the PureCol® EZ Gel solution.
  3. To form gel, warm to 37°C. The beginning of gelation will occur within 40 minutes, but allow approximately 90 to minutes for firm gel formation.

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