Geneva Biotech MultiBacMam™
英文名：MultiBacMam
货号：MultiBacMam
规格：MultiBacMam™ Kit + Competent Cells
品牌： Geneva Biotech
报价：价格面议
商品描述
BacMam是一种越来越流行的系统，它促进异源dna向广泛的哺乳动物转导目标的传递。杆状病毒是以杆状病毒为载体，由于杆状病毒具有较大的异源DNA携带能力（>40kbp），广泛的哺乳动物细胞类型对杆状病毒的吸收，以及在过去20年发展到现在的复杂分子生物学技术，因此与其他哺乳动物的转染方法相比，杆状病毒具有明显的优势。支持杆状病毒表达载体的产生。
MULTIBACMAM™是第一个专门设计用于哺乳动物细胞中多蛋白共表达的模块化、基于DNA重组的多表达盒组装的BACMAM系统。MULTIBACMAM™使用与MULTIBACM™相同的DNA重组技术，但使用哺乳动物启动子和“哺乳动物化”杆状病毒-在杆状病毒表面显示一个VSV肽，可将病毒摄取量增加一个数量级，以及一个稳定整合的荧光蛋白，以简化对实验的监控。
MULTIBACMAM™转移载体是完全合成的，只包含功能性DNA，因此体积小且易于处理。MULTIBAMM™杆状病毒基因组（DH10EMBACVSV）正在不断开发，以去除有害/非必需基因（如v-cath和chia）和DNA重组热点，并包含一个集成的荧光蛋白表达盒，以便于监测病毒滴度。multiabacmam™病毒颗粒在其表面显示VSV肽，与以前的bacmam病毒系统相比，将进入哺乳动物细胞的效率提高约1个数量级。
Mansouri等人2016（Nature Communications 2016，见下文参考下载链接）开发了一种用于初级细胞的特定转移载体子集，称为“多质体”。
为了提高多倍技术，日内瓦生物技术公司和合作者正在开发一系列额外的初级细胞特异性转导系统，专门研究难转导的细胞类型。
载体与多原载体一起允许您在所需的细胞模型中运行所需的分析。这项技术是第一个允许多种DNA编码因子在一个载体上被轻易地传递到哺乳动物细胞、组织或有机体的基因传递系统。它是第一个允许在哺乳动物细胞组织或生物体中表达多组分途径（例如信号级联）或复杂靶点（例如用于药物发现的多组分离子通道），而不需要复杂的多载体共转染。
BacMam is an increasingly popular system that facilitates delivery of heterologous DNAs to broad range of mammalian transduction targets. BacMam uses baculovirus as a vector, and has distinct advantages over other mammalian transfection methods given the large heterologous DNA carrying capacity of baculovirus (>40kbp), the uptake of baculovirus by a broad range of mammalian cell types, and the sophisticated molecular biology technologies that have evolved over the last 20 years to support generation of baculovirus expression vectors.
MultiBacMam™ is the first BacMam system designed specifically to allow modular, DNA recombination based assembly of multiexpression cassettes for multiprotein co-expression in mammalian cells. MultiBacMam™ uses the same DNA recombination technologies as MultiBac™, but with mammalian promoters, and a "mammalianized" baculovirus- displaying a VSV peptide on the baculovirus surface that increases virus uptake by an order of magnitude, and a stably integrated fluorescent protein to simplify monitoring of experiments.
MultiBacMam™ transfer vectors are fully synthetic, comprising only functional DNA, and as a result are small and easy to handle. The MultiBacMam™ baculovirus genome (DH10EMBacVSV) is under continuous development to remove harmful/non-essential genes (e.g. v-cath and chiA) and DNA recombination hotspots, and contains an integrated fluorescent protein expression cassette to allow for ease of monitoring virus titer. The MultiBacMam™ virus particle displays the VSV peptide on its surface to increase efficiency of entry into mammalian cells by around 1 order of magnitude compared to prior BacMam virus systems.
A specific subset of transfer vectors for use in primary cells called “MutiPrime” were developed by Mansouri et al 2016 (Nature Communications 2016, see reference download link below).
To advance the MultiPrime technology, Geneva Biotech and collaborators are developing a series of additional primary cell-specific transduction systems which specialize in tough to transduce cell types.
MultiBacMam™ vectors together with the MultiPrime vectors allows you to run the assays you need in the cellular models you want. This technology is first gene delivery system that easily allows for multiple DNA-encoded factors to be delivered on one vector into mammalian cells, tissues, or organisms. It is the first to allow expression of multi-component pathways (e.g. signal cascades), or complex targets such as multicomponent ion channels for drug discovery in mammalian cells tissues or organisms, all without the need for complicated multi vector co-transfections.
1.设计并克隆你感兴趣的基因（gois）到multibacmam™受体和供体载体中。
2.匹配并混合您的GoI，然后（重新）将它们组合成一个结构
3.使用独特的抗生素标记组合选择您的结构
4.将整个GOIS组件转移到MultiBacmam™Bacmid中。
5.选择并扩增含有多bacmam™bacmid的基因。
6.用纯化的multibamm™bacmid导入昆虫细胞
7.在昆虫细胞中扩增杆状病毒并收集所需的7级病毒。
8.用杆状病毒导入哺乳动物细胞
9.药物发现、生物生产、干细胞分化等。
1.Design and clone your gene(s) of interest (GOIs) into MultiBacMam™ acceptor and donor vectors
2.Match and mix your GOIs and then (re)combine them into one construct
3.Select your construct using unique combination of antibiotic markers
4.Transfer the entire GOIs-assembly into the MultiBacMam™ bacmid
5.Select and amplify your gene-containing MultiBacMam™ bacmid
6.Transfect insect cells with purified MultiBacMam™ bacmid
Amplify baculovirus in insect cells and collect desired 7.scale of virus
8.Transfect mammalian cells with baculovirus
9.Drug discovery, bioproduction, stem cell differentiation etc.
在Mansouri-et-al-2016-Nature-Communications（链接下方）中描述了将多基因读数传递到不同哺乳动物和初级细胞系的第一种方法。
The first method for delivering a multigene readout into diverse mammalian and primary cells lines is described in Mansouri-et-al-2016-Nature-Communications (below link).
Kits
MultiBacMam™ Kit + Competent Cells
Competent Cells
DH10EMBacVSV (12 x 100µl)
ig™ 10B (12 X 100µl)
PirHC (12 x 100µl)
Plasmids
pACEMam1 (5µg)
pACEMam2 (5µg)
pMDC (5µg)
pMDK (5µg)
pMDS (5µg)